Reviewed, UniProtKB/Swiss-Prot P41750 (PGLRB_ASPFL)

Names and origin

Protein names

Recommended name:
    Polygalacturonase B
    EC=3.2.1.15
Alternative name(s):
    Pectinase
    PGL
    P1/P3

Gene names

Name:

pecB

Organism

Aspergillus flavus

Taxonomic identifier

5059 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 30	13	Potential		PRO_0000024766
	Chain	31 – 366	336	Polygalacturonase B		PRO_0000024767
Sites
	Active site	205	1	Proton donor By similarity
	Active site	227	1	By similarity
Amino acid modifications
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
	Disulfide bond	34 ↔ 49		By similarity
	Disulfide bond	207 ↔ 223		By similarity
	Disulfide bond	333 ↔ 338		By similarity
	Disulfide bond	357 ↔ 366		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41750-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C8144083E1C4FB36	FASTA	366	37,877
	10 20 30 40 50 60 MHFQLLVLAP LALSSAAAPA PSRTSELVER GSSCTFTSAA QASASAKSCS NIVLKNIAVP 70 80 90 100 110 120 AGETLDLSKA KDGATITFEG TTTFGYKEWK GPLIRFGGNK ITVTQAAAVI DVQGSRWWDG 130 140 150 160 170 180 KGPNGGKTKP KFIQYPQLES PTITGLHVKN SPVQVFSVQG NDVHLTDITI DNSDGDNNGG 190 200 210 220 230 240 HNTDAFDVSE SNGVYITGAN VKNQDDCLAI NSGENIEFTG ATCSGGHGIS IGSIGNRDTN 250 260 270 280 290 300 TVKNVKVADS TVVDSDNGIR IKTISGATGS VSGVTYENIT LKNIKKNGIV IELDYKNGGP 310 320 330 340 350 360 TGKPTTGVPI TDLTVNGVTG SVASKATPVY ILCGKGSCSD WTWKGVSISG GKKSDKCQNI PSGASC

References

[1]	"Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus." Whitehead M.P., Shieh M.T., Cleveland T.E., Cary J.W., Dean R.A. Appl. Environ. Microbiol. 61:3316-3322(1995) [PubMed: 7574642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05020 Unassigned DNA. Translation: AAA85280.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 2238.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLRB_ASPFL

Accession

Primary (citable) accession number: P41750

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)