P41749 (PGLRA_ASPFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endopolygalacturonase A
EC=3.2.1.15

Alternative name(s):
P2C
PGL
Pectinase A
Polygalacturonase A

Gene names

Name:	pgaA
Synonyms:	pecA

Organism

Aspergillus flavus

Taxonomic identifier

5059 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Induction	Expressed in both pectin- and glucose-grown mycelia. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 28	8	Potential		PRO_0000024764
	Chain	29 – 363	335	Endopolygalacturonase A		PRO_0000024765
Regions
	Repeat	158 – 187	30	PbH1 1
	Repeat	188 – 209	22	PbH1 2
	Repeat	210 – 230	21	PbH1 3
	Repeat	239 – 260	22	PbH1 4
	Repeat	268 – 290	23	PbH1 5
	Repeat	302 – 347	46	PbH1 6
Sites
	Active site	202	1	Proton donor By similarity
	Active site	224	1	By similarity
Amino acid modifications
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 46		By similarity
	Disulfide bond	204 ↔ 220		By similarity
	Disulfide bond	330 ↔ 335		By similarity
	Disulfide bond	354 ↔ 363		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41749 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A6D44A870FD7B30F	FASTA	363	37,646
	10 20 30 40 50 60 MQLLQSSVIA ATVGAALVAA VPVELEARDS CTFTSAADAK SGKTSCSTIT LSNIEVPAGE 70 80 90 100 110 120 TLDLTGLNDG TTVIFSGETT FGYKEWEGPL ISVSGTNIKV QQASGAKIDG DGSRWWDGKG 130 140 150 160 170 180 GNGGKTKPKF CYVHKLDSSS ITGLQIYNTP VQGFSIQSDN LNITDVTIDN SAGTAEGHNT 190 200 210 220 230 240 DAFDVGSSTY INIDGATVYN QDDCLAINSG SHITFTNGYC DGGHGLSIGS VGGRSDNTVE 250 260 270 280 290 300 DVTISNSKVV NSQNGVRIKT VYDATGTVSN VKFEDITLSG ITKYGLIVEQ DYENGSPTGT 310 320 330 340 350 360 PTNGIKVSDI TFDKVTGTVE SDATDIYILC GSGSCTDWTW SGVSITGGKT SSKCENVPTG ASC

References

[1]	"Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus." Whitehead M.P., Shieh M.T., Cleveland T.E., Cary J.W., Dean R.A. Appl. Environ. Microbiol. 61:3316-3322(1995) [PubMed: 7574642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: 70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05015 mRNA. Translation: AAA85279.1.
3D structure databases
ProteinModelPortal	P41749.
SMR	P41749. Positions 30-361.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLRA_ASPFL

Accession

Primary (citable) accession number: P41749

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 31, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program