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Protein

Aspergillopepsin-1

Gene

pepA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A (By similarity). Can catalyze hydrolysis of the major structural proteins of basement membrane, elastin, collagen, and laminin. Thought to play a significant role in virulence (PubMed:7558282).By similarity1 Publication

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei284PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: AspGD

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • proteolysis Source: AspGD

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processVirulence

Protein family/group databases

MEROPSiA01.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspergillopepsin-1Curated (EC:3.4.23.181 Publication)
Alternative name(s):
Aspartic protease pepA
Aspergillopepsin F1 Publication
Aspergillopepsin I
Aspergillopeptidase A
Gene namesi
Name:pepA
ORF Names:AFUA_5G13300
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:Afu5g13300.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: AspGD

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3108. Asp f 10.0101.
63. Asp f 10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002592630 – 70Activation peptideBy similarityAdd BLAST41
ChainiPRO_000002592771 – 395Aspergillopepsin-1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi320 ↔ 355PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP41748.
SMRiP41748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 392Peptidase A1PROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484.
InParanoidiP41748.
KOiK06004.
OMAiINYGPVT.
OrthoDBiEOG092C49YE.

Family and domain databases

CDDicd06097. Aspergillopepsin_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR034163. Aspergillopepsin-like_cat_dom.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKVTAV VVGLSTIVSA VPVVQPRKGF TINQVARPVT NKKTVNLPAV
60 70 80 90 100
YANALTKYGG TVPDSVKAAA SSGSAVTTPE QYDSEYLTPV KVGGTTLNLD
110 120 130 140 150
FDTGSADLWV FSSELSASQS SGHAIYKPSA NAQKLNGYTW KIQYGDGSSA
160 170 180 190 200
SGDVYKDTVT VGGVTAQSQA VEAASHISSQ FVQDKDNDGL LGLAFSSINT
210 220 230 240 250
VSPRPQTTFF DTVKSQLDSP LFAVTLKYHA PGTYDFGYID NSKFQGELTY
260 270 280 290 300
TDVDSSQGFW MFTADGYGVG NGAPNSNSIS GIADTGTTLL LLDDSVVADY
310 320 330 340 350
YRQVSGAKNS NQYGGYVFPC STKLPSFTTV IGGYNAVVPG EYINYAPVTD
360 370 380 390
GSSTCYGGIQ SNSGLGFSIF GDIFLKSQYV VFDSQGPRLG FAPQA
Length:395
Mass (Da):41,613
Last modified:January 10, 2006 - v2
Checksum:iF3D1CCC0C6814BA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 27SAVPVVQPR → MLSLWSSRA in AAB07619 (PubMed:7558282).Curated9
Sequence conflicti33 – 34NQ → FL in AAB07619 (PubMed:7558282).Curated2
Sequence conflicti154V → G in AAB07619 (PubMed:7558282).Curated1
Sequence conflicti204 – 211RPQTTFFD → SDYFLY in AAB07619 (PubMed:7558282).Curated8
Sequence conflicti276S → T in AAB07619 (PubMed:7558282).Curated1
Sequence conflicti348 – 359VTDGS…CYGGI → SLTQLYLLRRH in AAB07619 (PubMed:7558282).CuratedAdd BLAST12
Sequence conflicti393 – 395PQA → LRH (PubMed:7558282).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31490 Genomic DNA. Translation: AAB07619.1.
X85092 Genomic DNA. Translation: CAA59419.1.
AAHF01000003 Genomic DNA. Translation: EAL91286.1.
RefSeqiXP_753324.1. XM_748231.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00006142; CADAFUAP00006142; CADAFUAG00006142.
GeneIDi3510846.
KEGGiafm:AFUA_5G13300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31490 Genomic DNA. Translation: AAB07619.1.
X85092 Genomic DNA. Translation: CAA59419.1.
AAHF01000003 Genomic DNA. Translation: EAL91286.1.
RefSeqiXP_753324.1. XM_748231.1.

3D structure databases

ProteinModelPortaliP41748.
SMRiP41748.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3108. Asp f 10.0101.
63. Asp f 10.
MEROPSiA01.026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00006142; CADAFUAP00006142; CADAFUAG00006142.
GeneIDi3510846.
KEGGiafm:AFUA_5G13300.

Organism-specific databases

EuPathDBiFungiDB:Afu5g13300.

Phylogenomic databases

HOGENOMiHOG000167484.
InParanoidiP41748.
KOiK06004.
OMAiINYGPVT.
OrthoDBiEOG092C49YE.

Family and domain databases

CDDicd06097. Aspergillopepsin_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR034163. Aspergillopepsin-like_cat_dom.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA_ASPFU
AccessioniPrimary (citable) accession number: P41748
Secondary accession number(s): Q12547, Q4WVU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2006
Last modified: June 7, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.