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Protein

Aspergillopepsin-1

Gene

pepA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A (By similarity). Can catalyze hydrolysis of the major structural proteins of basement membrane, elastin, collagen, and laminin. Thought to play a significant role in virulence (PubMed:7558282).By similarity1 Publication

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei284PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: AspGD

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • proteolysis Source: AspGD

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processVirulence

Protein family/group databases

MEROPSiA01.026

Names & Taxonomyi

Protein namesi
Recommended name:
Aspergillopepsin-1Curated (EC:3.4.23.181 Publication)
Alternative name(s):
Aspartic protease pepA
Aspergillopepsin F1 Publication
Aspergillopepsin I
Aspergillopeptidase A
Gene namesi
Name:pepA
ORF Names:AFUA_5G13300
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu5g13300

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3108 Asp f 10.0101
63 Asp f 10

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002592630 – 70Activation peptideBy similarityAdd BLAST41
ChainiPRO_000002592771 – 395Aspergillopepsin-1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi320 ↔ 355PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00005960

Structurei

3D structure databases

ProteinModelPortaliP41748
SMRiP41748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 392Peptidase A1PROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484
InParanoidiP41748
KOiK06004
OMAiINYGPVT
OrthoDBiEOG092C49YE

Family and domain databases

CDDicd06097 Aspergillopepsin_like, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR034163 Aspergillopepsin-like_cat_dom
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKVTAV VVGLSTIVSA VPVVQPRKGF TINQVARPVT NKKTVNLPAV
60 70 80 90 100
YANALTKYGG TVPDSVKAAA SSGSAVTTPE QYDSEYLTPV KVGGTTLNLD
110 120 130 140 150
FDTGSADLWV FSSELSASQS SGHAIYKPSA NAQKLNGYTW KIQYGDGSSA
160 170 180 190 200
SGDVYKDTVT VGGVTAQSQA VEAASHISSQ FVQDKDNDGL LGLAFSSINT
210 220 230 240 250
VSPRPQTTFF DTVKSQLDSP LFAVTLKYHA PGTYDFGYID NSKFQGELTY
260 270 280 290 300
TDVDSSQGFW MFTADGYGVG NGAPNSNSIS GIADTGTTLL LLDDSVVADY
310 320 330 340 350
YRQVSGAKNS NQYGGYVFPC STKLPSFTTV IGGYNAVVPG EYINYAPVTD
360 370 380 390
GSSTCYGGIQ SNSGLGFSIF GDIFLKSQYV VFDSQGPRLG FAPQA
Length:395
Mass (Da):41,613
Last modified:January 10, 2006 - v2
Checksum:iF3D1CCC0C6814BA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 27SAVPVVQPR → MLSLWSSRA in AAB07619 (PubMed:7558282).Curated9
Sequence conflicti33 – 34NQ → FL in AAB07619 (PubMed:7558282).Curated2
Sequence conflicti154V → G in AAB07619 (PubMed:7558282).Curated1
Sequence conflicti204 – 211RPQTTFFD → SDYFLY in AAB07619 (PubMed:7558282).Curated8
Sequence conflicti276S → T in AAB07619 (PubMed:7558282).Curated1
Sequence conflicti348 – 359VTDGS…CYGGI → SLTQLYLLRRH in AAB07619 (PubMed:7558282).CuratedAdd BLAST12
Sequence conflicti393 – 395PQA → LRH (PubMed:7558282).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31490 Genomic DNA Translation: AAB07619.1
X85092 Genomic DNA Translation: CAA59419.1
AAHF01000003 Genomic DNA Translation: EAL91286.1
RefSeqiXP_753324.1, XM_748231.1

Genome annotation databases

EnsemblFungiiCADAFUAT00006142; CADAFUAP00006142; CADAFUAG00006142
GeneIDi3510846
KEGGiafm:AFUA_5G13300

Similar proteinsi

Entry informationi

Entry nameiPEPA_ASPFU
AccessioniPrimary (citable) accession number: P41748
Secondary accession number(s): Q12547, Q4WVU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2006
Last modified: April 25, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health