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Protein

Aspartic protease pep1

Gene

pep1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. Can catalyze hydrolysis of the major structural proteins of basement membrane, elastin, collagen, and laminin. Thought to play a significant role in virulence (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.

pH dependencei

Optimum pH is 5.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021PROSITE-ProRule annotation
Active sitei284 – 2841PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • endopeptidase activity Source: ASPGD

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • proteolysis Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiA01.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic protease pep1 (EC:3.4.23.18)
Alternative name(s):
Aspergillopepsin A
Aspergillopepsin I
Gene namesi
Name:pep1
Synonyms:pepA
ORF Names:AFUA_5G13300
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:Afu5g13300.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei3108. Asp f 10.0101.
63. Asp f 10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Propeptidei30 – 7041Activation peptideBy similarityPRO_0000025926Add
BLAST
Chaini71 – 395325Aspartic protease pep1PRO_0000025927Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi320 ↔ 355By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP41748.
SMRiP41748. Positions 71-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 392307Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484.
InParanoidiP41748.
KOiK06004.
OMAiFTINQVA.
OrthoDBiEOG092C49YE.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKVTAV VVGLSTIVSA VPVVQPRKGF TINQVARPVT NKKTVNLPAV
60 70 80 90 100
YANALTKYGG TVPDSVKAAA SSGSAVTTPE QYDSEYLTPV KVGGTTLNLD
110 120 130 140 150
FDTGSADLWV FSSELSASQS SGHAIYKPSA NAQKLNGYTW KIQYGDGSSA
160 170 180 190 200
SGDVYKDTVT VGGVTAQSQA VEAASHISSQ FVQDKDNDGL LGLAFSSINT
210 220 230 240 250
VSPRPQTTFF DTVKSQLDSP LFAVTLKYHA PGTYDFGYID NSKFQGELTY
260 270 280 290 300
TDVDSSQGFW MFTADGYGVG NGAPNSNSIS GIADTGTTLL LLDDSVVADY
310 320 330 340 350
YRQVSGAKNS NQYGGYVFPC STKLPSFTTV IGGYNAVVPG EYINYAPVTD
360 370 380 390
GSSTCYGGIQ SNSGLGFSIF GDIFLKSQYV VFDSQGPRLG FAPQA
Length:395
Mass (Da):41,613
Last modified:January 10, 2006 - v2
Checksum:iF3D1CCC0C6814BA9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 279SAVPVVQPR → MLSLWSSRA in AAB07619 (PubMed:7558282).Curated
Sequence conflicti33 – 342NQ → FL in AAB07619 (PubMed:7558282).Curated
Sequence conflicti154 – 1541V → G in AAB07619 (PubMed:7558282).Curated
Sequence conflicti204 – 2118RPQTTFFD → SDYFLY in AAB07619 (PubMed:7558282).Curated
Sequence conflicti276 – 2761S → T in AAB07619 (PubMed:7558282).Curated
Sequence conflicti348 – 35912VTDGS…CYGGI → SLTQLYLLRRH in AAB07619 (PubMed:7558282).CuratedAdd
BLAST
Sequence conflicti393 – 3953PQA → LRH (PubMed:7558282).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31490 Genomic DNA. Translation: AAB07619.1.
X85092 Genomic DNA. Translation: CAA59419.1.
AAHF01000003 Genomic DNA. Translation: EAL91286.1.
RefSeqiXP_753324.1. XM_748231.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00006142; CADAFUAP00006142; CADAFUAG00006142.
GeneIDi3510846.
KEGGiafm:AFUA_5G13300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31490 Genomic DNA. Translation: AAB07619.1.
X85092 Genomic DNA. Translation: CAA59419.1.
AAHF01000003 Genomic DNA. Translation: EAL91286.1.
RefSeqiXP_753324.1. XM_748231.1.

3D structure databases

ProteinModelPortaliP41748.
SMRiP41748. Positions 71-395.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3108. Asp f 10.0101.
63. Asp f 10.
MEROPSiA01.026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00006142; CADAFUAP00006142; CADAFUAG00006142.
GeneIDi3510846.
KEGGiafm:AFUA_5G13300.

Organism-specific databases

EuPathDBiFungiDB:Afu5g13300.

Phylogenomic databases

HOGENOMiHOG000167484.
InParanoidiP41748.
KOiK06004.
OMAiFTINQVA.
OrthoDBiEOG092C49YE.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA_ASPFU
AccessioniPrimary (citable) accession number: P41748
Secondary accession number(s): Q12547, Q4WVU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2006
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.