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Protein

Aryl hydrocarbon receptor

Gene

Ahr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.1 Publication

GO - Molecular functioni

  • aryl hydrocarbon receptor activity Source: RGD
  • DNA binding Source: RGD
  • E-box binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell cycle Source: UniProtKB
  • cellular response to glucocorticoid stimulus Source: RGD
  • circadian regulation of gene expression Source: UniProtKB
  • intracellular receptor signaling pathway Source: GOC
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • ovarian follicle development Source: RGD
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • response to estradiol Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to stress Source: UniProtKB
  • response to xenobiotic stimulus Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor, Repressor

Keywords - Biological processi

Biological rhythms, Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl hydrocarbon receptor
Short name:
Ah receptor
Short name:
AhR
Gene namesi
Name:Ahr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2074. Ahr.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5400.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 99By similarityPRO_0000013464
Chaini10 – 853844Aryl hydrocarbon receptorPRO_0000013465Add
BLAST

Proteomic databases

PaxDbiP41738.
PRIDEiP41738.

PTM databases

iPTMnetiP41738.
PhosphoSiteiP41738.

Expressioni

Tissue specificityi

Expressed in all tissues tested including brain, heart, kidney, liver, lung, spleen, skeletal muscle and thymus.2 Publications

Inductioni

Induced or repressed by TGF-beta in a cell-specific fashion. Repressed by dioxin, retinoic acid, and TPA.1 Publication

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. In the nucleus, heterodimer of AHR and ARNT. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with MYBBP1A, NEDD8 and IVNS1ABP. Interacts with ARNTL/BMAL1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ArntP417392EBI-1162880,EBI-1162920

Protein-protein interaction databases

BioGridi247719. 4 interactions.
IntActiP41738. 2 interactions.
STRINGi10116.ENSRNOP00000006618.

Chemistry

BindingDBiP41738.

Structurei

3D structure databases

ProteinModelPortaliP41738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 7954bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini116 – 17964PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 34068PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini346 – 38439PACAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi596 – 63742Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3560. Eukaryota.
ENOG410XRZH. LUCA.
HOGENOMiHOG000252935.
HOVERGENiHBG007313.
InParanoidiP41738.
PhylomeDBiP41738.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR033348. AHR.
IPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
[Graphical view]
PANTHERiPTHR10649:SF9. PTHR10649:SF9. 1 hit.
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P41738-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGANITYA SRKRRKPVQK TVKPVPAEGI KSNPSKRHRD RLNTELDRLA
60 70 80 90 100
SLLPFPQDVI NKLDKLSVLR LSVSYLRAKS FFDVALKSTP ADRSRGQDQC
110 120 130 140 150
RAQVRDWQDL QEGEFLLQAL NGFVLVVTAD ALVFYASSTI QDYLGFQQSD
160 170 180 190 200
VIHQSVYELI HTEDRAEFQR QLHWALNPSQ CTDSAQGVDE THGLPQPAVY
210 220 230 240 250
YTPDQLPPEN TAFMERCFRC RLRCLLDNSS GFLAMNFQGR LKYLHGQNKK
260 270 280 290 300
GKDGALLPPQ LALFAIATPL QPPSILEIRT KNFIFRTKHK LDFTPIGCDA
310 320 330 340 350
KGQLILGYTE VELCNKGSGY QFIHAADMLH CAESHIRMIK TGESGMTVFR
360 370 380 390 400
LLAKHSRWRW VQSNARLIYR NGRPDYIIAT QRPLTDEEGR EHLQKRSMTL
410 420 430 440 450
PFMFATGEAV LYEISSPFSP IMDPLPIRTK SNTSRKDWAP QSTPSKDSFH
460 470 480 490 500
PNSLMSALIQ QDESIYLCPP SSPAPLDSHF LMDSMSECGS WQGSFAVASN
510 520 530 540 550
EALLKHEEIR HTQDVNLTLS GGPSELFPDN KNNDLYSIMR NLGIDFEDIR
560 570 580 590 600
SMQNEEFFRT DSSGEVDFKD IDITDEILTY VQDSLNNSTL LNSACQQQPV
610 620 630 640 650
SQHLSCMLQE RLQLEQQQQL QQQHPTQTLE PQRQLCQVEV PQHELGQKTK
660 670 680 690 700
HMQVNGMFAS WNPAPPVSFS CPQQERKHYS LFSGLQGTAQ EFPYKSEVDS
710 720 730 740 750
MPYTQNFAPC NQSLLPEHSK GTQLDFPGRD FERSLHPNAS NLEDFVSCLQ
760 770 780 790 800
VPENQRHGIN SQSAMVSPQA YYAGAMSMYQ CQAGPQHTPV DQMQYSPEIP
810 820 830 840 850
GSQAFLSKFQ SPSILNEAYS ADLSSIGHLQ TAAHLPRLAE AQPLPDITPS

GFL
Length:853
Mass (Da):96,227
Last modified:March 25, 2003 - v2
Checksum:i4EED0EE795FF1327
GO
Isoform 2 (identifier: P41738-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     142-376: Missing.

Show »
Length:618
Mass (Da):69,332
Checksum:i2BA470B081D008B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741S → T in AAA56897 (PubMed:8065918).Curated
Sequence conflicti659 – 6591A → V in AAA56897 (PubMed:8065918).Curated
Sequence conflicti679 – 6824YSLF → ICLL in AAA56897 (PubMed:8065918).Curated
Sequence conflicti739 – 7391A → V in AAA56897 (PubMed:8065918).Curated
Sequence conflicti744 – 7441D → E in AAA56897 (PubMed:8065918).Curated
Sequence conflicti770 – 7701A → G in AAA56897 (PubMed:8065918).Curated
Sequence conflicti787 – 7871H → D in AAA56897 (PubMed:8065918).Curated
Sequence conflicti794 – 7941Q → H in AAA19451 (PubMed:7812217).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti497 – 4971V → A in strain: Han/Wistar, Han/Wistar-dv, Han/Wistar-siv and Han/Wistar-liv. 1 Publication
Natural varianti766 – 80843Missing in strain: Han/Wistar-dv.
Add
BLAST
Natural varianti809 – 85345FQSPS…PSGFL → IRAFYRE in strain: Han/Wistar-siv and Han/Wistar-liv.
Add
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei142 – 376235Missing in isoform 2. 1 PublicationVSP_002091Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09000 mRNA. Translation: AAA56897.1.
U04860 mRNA. Translation: AAA19451.1.
AF082124 mRNA. Translation: AAC35168.1.
AF082125 mRNA. Translation: AAC35169.1.
AF082126 mRNA. Translation: AAC35170.1.
PIRiS58375.
UniGeneiRn.91370.

Genome annotation databases

UCSCiRGD:2074. rat. [P41738-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09000 mRNA. Translation: AAA56897.1.
U04860 mRNA. Translation: AAA19451.1.
AF082124 mRNA. Translation: AAC35168.1.
AF082125 mRNA. Translation: AAC35169.1.
AF082126 mRNA. Translation: AAC35170.1.
PIRiS58375.
UniGeneiRn.91370.

3D structure databases

ProteinModelPortaliP41738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247719. 4 interactions.
IntActiP41738. 2 interactions.
STRINGi10116.ENSRNOP00000006618.

Chemistry

BindingDBiP41738.
ChEMBLiCHEMBL5400.

PTM databases

iPTMnetiP41738.
PhosphoSiteiP41738.

Proteomic databases

PaxDbiP41738.
PRIDEiP41738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2074. rat. [P41738-1]

Organism-specific databases

RGDi2074. Ahr.

Phylogenomic databases

eggNOGiKOG3560. Eukaryota.
ENOG410XRZH. LUCA.
HOGENOMiHOG000252935.
HOVERGENiHBG007313.
InParanoidiP41738.
PhylomeDBiP41738.

Miscellaneous databases

NextBioi607689.
PROiP41738.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR033348. AHR.
IPR011598. bHLH_dom.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR013655. PAS_fold_3.
[Graphical view]
PANTHERiPTHR10649:SF9. PTHR10649:SF9. 1 hit.
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
PF08447. PAS_3. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Tissue specific expression of the rat Ah-receptor and ARNT mRNAs."
    Carver L.A., Hogenesch J.B., Bradfield C.A.
    Nucleic Acids Res. 22:3038-3044(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: Fischer 344 and Sprague-Dawley.
    Tissue: Liver and Placenta.
  2. "Dioxin-dependent, DNA sequence-specific binding of a multiprotein complex containing the Ah receptor."
    Elferink C.J., Whitlock J.P. Jr.
    Receptor 4:157-173(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 236-251 AND 702-708, FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "Point mutation in intron sequence causes altered carboxyl-terminal structure in the aryl hydrocarbon receptor of the most 2,3,7,8-tetrachlorodibenzo-p-dioxin-resistant rat strain."
    Pohjanvirta R., Wong J.M.Y., Li W., Harper P.A., Tuomisto J., Okey A.B.
    Mol. Pharmacol. 54:86-93(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS HAN/WISTAR ALA-497; HAN/WISTAR-DV; HAN/WISTAR-SIV AND HAN/WISTAR-LIV.
    Strain: Han/Wistar, Long Evans and Sprague-Dawley.
  4. "In vivo up-regulation of aryl hydrocarbon receptor expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a dioxin-resistant rat model."
    Franc M.A., Pohjanvirta R., Tuomisto J., Okey A.B.
    Biochem. Pharmacol. 62:1565-1578(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Role of the aryl hydrocarbon receptor in cell cycle regulation."
    Puga A., Xia Y., Elferink C.
    Chem. Biol. Interact. 141:117-130(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON ROLE IN CELL CYCLE.

Entry informationi

Entry nameiAHR_RAT
AccessioniPrimary (citable) accession number: P41738
Secondary accession number(s): O88930, O89105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: March 25, 2003
Last modified: May 11, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.