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Protein

5-demethoxyubiquinone hydroxylase, mitochondrial

Gene

CAT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of 2-hexaprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis (PubMed:8621692, PubMed:9452453, PubMed:16624818). Has also a structural role in the COQ enzyme complex, stabilizing COQ3 and COQ4 polypeptides (PubMed:16624818).UniRule annotation3 Publications

Caution

Was originally thought to be involved in carbon catabolite repression (PubMed:8557031). It has later been demonstrated that the catabolite-regulation defect in COQ7 mutants was a secondary effect of the respiration deficieny in ubiquinone-deficient mutants and could be rescued by the addition of exogenous ubiquinone (PubMed:9452453).2 Publications

Cofactori

Fe cationUniRule annotationNote: Binds 2 iron ions per subunit.UniRule annotation

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Iron 1UniRule annotation1
Metal bindingi95Iron 1UniRule annotation1
Metal bindingi95Iron 2UniRule annotation1
Metal bindingi98Iron 1UniRule annotation1
Metal bindingi147Iron 2UniRule annotation1
Metal bindingi194Iron 1UniRule annotation1
Metal bindingi194Iron 2UniRule annotation1
Metal bindingi197Iron 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ubiquinone biosynthetic process Source: SGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processUbiquinone biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-164
YEAST:MONOMER3O-164
UniPathwayiUPA00232

Names & Taxonomyi

Protein namesi
Recommended name:
5-demethoxyubiquinone hydroxylase, mitochondrialUniRule annotation1 Publication (EC:1.14.13.-UniRule annotation1 Publication)
Short name:
DMQ hydroxylaseUniRule annotation
Alternative name(s):
Catabolite repression protein 51 Publication
Ubiquinone biosynthesis monooxygenase COQ7UniRule annotation1 Publication
Gene namesi
Name:CAT51 Publication
Synonyms:COQ71 PublicationUniRule annotation
Ordered Locus Names:YOR125CImported
ORF Names:O3284, YOR3284C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR125C
SGDiS000005651 CAT5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194E → K: Lacks ubiquinone and accumulates the intermediate DMQH2, causing respiratory deficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 15MitochondrionUniRule annotationAdd BLAST15
ChainiPRO_000008933016 – 2335-demethoxyubiquinone hydroxylase, mitochondrialAdd BLAST218

Proteomic databases

MaxQBiP41735
PaxDbiP41735
PRIDEiP41735

Interactioni

Subunit structurei

Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi34520390 interactors.
DIPiDIP-6435N
IntActiP41735 14 interactors.
MINTiP41735
STRINGi4932.YOR125C

Structurei

3D structure databases

ProteinModelPortaliP41735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the COQ7 family.UniRule annotationCurated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000014520
HOGENOMiHOG000184972
InParanoidiP41735
KOiK06134
OMAiTIGHMWE
OrthoDBiEOG092C4BLJ

Family and domain databases

CDDicd01042 DMQH, 1 hit
HAMAPiMF_01658 COQ7, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR011566 Ubq_synth_Coq7
PANTHERiPTHR11237 PTHR11237, 1 hit
PfamiView protein in Pfam
PF03232 COQ7, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRVSVFKP ASRGFSVLSS LKITEHTSAK HTEKPEHAPK CQNLSDAQAA
60 70 80 90 100
FLDRVIRVDQ AGELGADYIY AGQYFVLAHR YPHLKPVLKH IWDQEIHHHN
110 120 130 140 150
TFNNLQLKRR VRPSLLTPLW KAGAFAMGAG TALISPEAAM ACTEAVETVI
160 170 180 190 200
GGHYNGQLRN LANQFNLERT DGTKGPSEEI KSLTSTIQQF RDDELEHLDT
210 220 230
AIKHDSYMAV PYTVITEGIK TICRVAIWSA ERI
Length:233
Mass (Da):26,060
Last modified:November 22, 2005 - v2
Checksum:i2686F44C5F17EFE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82930 Genomic DNA Translation: CAA58105.1
S81938 mRNA Translation: AAB36435.1
X90518 Genomic DNA Translation: CAA62119.1
X94335 Genomic DNA Translation: CAA64044.1
Z75033 Genomic DNA Translation: CAA99324.1
BK006948 Genomic DNA Translation: DAA10899.1
PIRiS49912
RefSeqiNP_014768.2, NM_001183544.1

Genome annotation databases

EnsemblFungiiYOR125C; YOR125C; YOR125C
GeneIDi854292
KEGGisce:YOR125C

Similar proteinsi

Entry informationi

Entry nameiCOQ7_YEAST
AccessioniPrimary (citable) accession number: P41735
Secondary accession number(s): D6W2I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 22, 2005
Last modified: February 28, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome