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Protein

Isoamyl acetate-hydrolyzing esterase

Gene

IAH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a crucial role in the hydrolysis of isoamyl acetate in sake mash.

Kineticsi

  1. KM=40.3 mM for isoamyl acetate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei12 – 121NucleophileBy similarity
    Binding sitei53 – 531Substrate; via amide nitrogenBy similarity
    Binding sitei83 – 831SubstrateBy similarity
    Active sitei187 – 1871By similarity
    Active sitei190 – 1901By similarity

    GO - Molecular functioni

    • hydrolase activity, acting on ester bonds Source: SGD

    GO - Biological processi

    • acetate metabolic process Source: SGD
    • lipid catabolic process Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YOR126C-MONOMER.
    SABIO-RKP41734.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoamyl acetate-hydrolyzing esterase (EC:3.1.-.-)
    Gene namesi
    Name:IAH1
    Synonyms:EST2
    Ordered Locus Names:YOR126C
    ORF Names:O3287, YOR3287C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR126C.
    SGDiS000005652. IAH1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Isoamyl acetate-hydrolyzing esterasePRO_0000084126Add
    BLAST

    Proteomic databases

    MaxQBiP41734.

    Interactioni

    Protein-protein interaction databases

    BioGridi34521. 46 interactions.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 118Combined sources
    Helixi12 – 154Combined sources
    Turni16 – 183Combined sources
    Helixi32 – 398Combined sources
    Turni40 – 434Combined sources
    Beta strandi44 – 507Combined sources
    Helixi56 – 6914Combined sources
    Beta strandi73 – 797Combined sources
    Turni82 – 854Combined sources
    Beta strandi86 – 894Combined sources
    Helixi95 – 11117Combined sources
    Beta strandi115 – 1195Combined sources
    Helixi126 – 1327Combined sources
    Helixi134 – 1385Combined sources
    Helixi145 – 16117Combined sources
    Helixi169 – 1779Combined sources
    Helixi178 – 1847Combined sources
    Beta strandi185 – 1917Combined sources
    Helixi193 – 21018Combined sources
    Helixi212 – 2143Combined sources
    Helixi216 – 2183Combined sources
    Helixi226 – 2283Combined sources
    Turni234 – 2385Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MILX-ray1.60A/B1-238[»]
    ProteinModelPortaliP41734.
    SMRiP41734. Positions 1-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP41734.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000008069.
    HOGENOMiHOG000214294.
    InParanoidiP41734.
    OMAiSGYNSTW.
    OrthoDBiEOG092C4VGR.

    Family and domain databases

    Gene3Di3.40.50.1110. 1 hit.
    InterProiIPR013830. SGNH_hydro.
    [Graphical view]
    PfamiPF13472. Lipase_GDSL_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52266. SSF52266. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P41734-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDYEKFLLFG DSITEFAFNT RPIEDGKDQY ALGAALVNEY TRKMDILQRG
    60 70 80 90 100
    FKGYTSRWAL KILPEILKHE SNIVMATIFL GANDACSAGP QSVPLPEFID
    110 120 130 140 150
    NIRQMVSLMK SYHIRPIIIG PGLVDREKWE KEKSEEIALG YFRTNENFAI
    160 170 180 190 200
    YSDALAKLAN EEKVPFVALN KAFQQEGGDA WQQLLTDGLH FSGKGYKIFH
    210 220 230
    DELLKVIETF YPQYHPKNMQ YKLKDWRDVL DDGSNIMS
    Length:238
    Mass (Da):27,346
    Last modified:November 1, 1995 - v1
    Checksum:i8C86C0B4CF49C50B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X82930 Genomic DNA. Translation: CAA58104.1.
    X94335 Genomic DNA. Translation: CAA64045.1.
    X92662 Genomic DNA. Translation: CAA63350.1.
    X90518 Genomic DNA. Translation: CAA62126.1.
    Z75034 Genomic DNA. Translation: CAA99325.1.
    AY557754 Genomic DNA. Translation: AAS56080.1.
    BK006948 Genomic DNA. Translation: DAA10900.1.
    PIRiS49911.
    RefSeqiNP_014769.3. NM_001183545.3.

    Genome annotation databases

    EnsemblFungiiYOR126C; YOR126C; YOR126C.
    GeneIDi854293.
    KEGGisce:YOR126C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X82930 Genomic DNA. Translation: CAA58104.1.
    X94335 Genomic DNA. Translation: CAA64045.1.
    X92662 Genomic DNA. Translation: CAA63350.1.
    X90518 Genomic DNA. Translation: CAA62126.1.
    Z75034 Genomic DNA. Translation: CAA99325.1.
    AY557754 Genomic DNA. Translation: AAS56080.1.
    BK006948 Genomic DNA. Translation: DAA10900.1.
    PIRiS49911.
    RefSeqiNP_014769.3. NM_001183545.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MILX-ray1.60A/B1-238[»]
    ProteinModelPortaliP41734.
    SMRiP41734. Positions 1-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi34521. 46 interactions.

    Proteomic databases

    MaxQBiP41734.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYOR126C; YOR126C; YOR126C.
    GeneIDi854293.
    KEGGisce:YOR126C.

    Organism-specific databases

    EuPathDBiFungiDB:YOR126C.
    SGDiS000005652. IAH1.

    Phylogenomic databases

    GeneTreeiENSGT00390000008069.
    HOGENOMiHOG000214294.
    InParanoidiP41734.
    OMAiSGYNSTW.
    OrthoDBiEOG092C4VGR.

    Enzyme and pathway databases

    BioCyciYEAST:YOR126C-MONOMER.
    SABIO-RKP41734.

    Miscellaneous databases

    EvolutionaryTraceiP41734.
    PROiP41734.

    Family and domain databases

    Gene3Di3.40.50.1110. 1 hit.
    InterProiIPR013830. SGNH_hydro.
    [Graphical view]
    PfamiPF13472. Lipase_GDSL_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52266. SSF52266. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiIAH1_YEAST
    AccessioniPrimary (citable) accession number: P41734
    Secondary accession number(s): D6W2I4, Q92313
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: September 7, 2016
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.