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P41731

- CD63_MOUSE

UniProt

P41731 - CD63_MOUSE

Protein

CD63 antigen

Gene

Cd63

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.5 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. cell-matrix adhesion Source: UniProtKB
    2. cell migration Source: UniProtKB
    3. cellular protein localization Source: MGI
    4. endosome to melanosome transport Source: UniProtKB
    5. pigmentation Source: UniProtKB
    6. pigment cell differentiation Source: UniProtKB
    7. pigment granule maturation Source: UniProtKB
    8. positive regulation of integrin-mediated signaling pathway Source: UniProtKB
    9. positive regulation of receptor internalization Source: UniProtKB
    10. protein transport Source: UniProtKB-KW
    11. regulation of rubidium ion transport Source: MGI
    12. regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD63 antigen
    Alternative name(s):
    CD_antigen: CD63
    Gene namesi
    Name:Cd63
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:99529. Cd63.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosomemultivesicular body By similarity. Melanosome By similarity
    Note: Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies By similarity.By similarity

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. endosome membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. intrinsic component of plasma membrane Source: UniProtKB
    5. late endosome Source: MGI
    6. late endosome membrane Source: UniProtKB
    7. lysosomal membrane Source: UniProtKB
    8. lysosome Source: MGI
    9. melanosome Source: UniProtKB-SubCell
    10. multivesicular body, internal vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Lysosome, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Mutant mice are viable and fertile, but display impaired water homeostasis, with increased urinary flow, increased water intake, reduced urine osmolality and increased fecal water content. Mice display inclusions in the principal cells in the renal collecting duct (PubMed:19075008). Mutant mice display variable graying of their coat color and a dramatic reduction in the number of melanosomes in the retinal pigment epithelium (PubMed:21962903). According to PubMed:21803846, mutant mice display a defect in the Selp-dependent attachment of leukocytes to endothelial cells. According to PubMed:23945142, mast cells from mutant mice show decreased degranulation and decreased release of TNF in response to Ms4a2/FceRI stimulation, but no difference in mast cell degranulation in response to other stimuli and no change in the release of IL6 and leukotriene C4.5 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 238237CD63 antigenPRO_0000219217Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi130 – 1301N-linked (GlcNAc...)1 Publication
    Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP41731.
    PaxDbiP41731.
    PRIDEiP41731.

    PTM databases

    PhosphoSiteiP41731.

    Expressioni

    Tissue specificityi

    Ubiquitous. Strongly expressed in kidney. Detected in spleen, bone marrow, peripheral blood mononuclear cells and macrophages.

    Gene expression databases

    ArrayExpressiP41731.
    BgeeiP41731.
    CleanExiMM_CD63.
    GenevestigatoriP41731.

    Interactioni

    Subunit structurei

    Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes By similarity.By similarity

    Protein-protein interaction databases

    IntActiP41731. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP41731.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1110CytoplasmicSequence Analysis
    Topological domaini33 – 5119ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini73 – 819CytoplasmicSequence Analysis
    Topological domaini103 – 203101ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini225 – 23814CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei52 – 7221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei204 – 22421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tetraspanin (TM4SF) family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268658.
    HOVERGENiHBG107306.
    InParanoidiP41731.
    KOiK06497.
    OMAiVTQGCGI.
    OrthoDBiEOG73BVDM.
    TreeFamiTF316345.

    Family and domain databases

    InterProiIPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view]
    PfamiPF00335. Tetraspannin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002419. Tetraspanin. 1 hit.
    PRINTSiPR00259. TMFOUR.
    SUPFAMiSSF48652. SSF48652. 1 hit.
    PROSITEiPS00421. TM4_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41731-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVEGGMKCV KFLLYVLLLA FCACAVGLIA IGVAVQVVLK QAITHETTAG    50
    SLLPVVIIAV GAFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAV 100
    AIAGYVFRDQ VKSEFNKSFQ QQMQNYLKDN KTATILDKLQ KENNCCGASN 150
    YTDWENIPGM AKDRVPDSCC INITVGCGND FKESTIHTQG CVETIAIWLR 200
    KNILLVAAAA LGIAFVEVLG IIFSCCLVKS IRSGYEVM 238
    Length:238
    Mass (Da):25,767
    Last modified:January 23, 2007 - v2
    Checksum:iB57E47B31E13EED8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16432 mRNA. Translation: BAA03904.1.
    BC008108 mRNA. Translation: AAH08108.1.
    BC012212 mRNA. Translation: AAH12212.1.
    BC083161 mRNA. Translation: AAH83161.1.
    CCDSiCCDS24297.1.
    PIRiS43511.
    RefSeqiNP_001036045.1. NM_001042580.1.
    NP_001269895.1. NM_001282966.1.
    NP_031679.1. NM_007653.3.
    UniGeneiMm.371552.
    Mm.416079.

    Genome annotation databases

    EnsembliENSMUST00000026407; ENSMUSP00000026407; ENSMUSG00000025351.
    ENSMUST00000105229; ENSMUSP00000100862; ENSMUSG00000025351.
    GeneIDi12512.
    KEGGimmu:12512.
    UCSCiuc007hou.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16432 mRNA. Translation: BAA03904.1 .
    BC008108 mRNA. Translation: AAH08108.1 .
    BC012212 mRNA. Translation: AAH12212.1 .
    BC083161 mRNA. Translation: AAH83161.1 .
    CCDSi CCDS24297.1.
    PIRi S43511.
    RefSeqi NP_001036045.1. NM_001042580.1.
    NP_001269895.1. NM_001282966.1.
    NP_031679.1. NM_007653.3.
    UniGenei Mm.371552.
    Mm.416079.

    3D structure databases

    ProteinModelPortali P41731.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P41731. 2 interactions.

    PTM databases

    PhosphoSitei P41731.

    Proteomic databases

    MaxQBi P41731.
    PaxDbi P41731.
    PRIDEi P41731.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026407 ; ENSMUSP00000026407 ; ENSMUSG00000025351 .
    ENSMUST00000105229 ; ENSMUSP00000100862 ; ENSMUSG00000025351 .
    GeneIDi 12512.
    KEGGi mmu:12512.
    UCSCi uc007hou.1. mouse.

    Organism-specific databases

    CTDi 967.
    MGIi MGI:99529. Cd63.

    Phylogenomic databases

    eggNOGi NOG268658.
    HOVERGENi HBG107306.
    InParanoidi P41731.
    KOi K06497.
    OMAi VTQGCGI.
    OrthoDBi EOG73BVDM.
    TreeFami TF316345.

    Miscellaneous databases

    NextBioi 281496.
    PROi P41731.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P41731.
    Bgeei P41731.
    CleanExi MM_CD63.
    Genevestigatori P41731.

    Family and domain databases

    InterProi IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view ]
    Pfami PF00335. Tetraspannin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002419. Tetraspanin. 1 hit.
    PRINTSi PR00259. TMFOUR.
    SUPFAMi SSF48652. SSF48652. 1 hit.
    PROSITEi PS00421. TM4_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages."
      Miyamoto M., Homma M., Hotta H.
      Biochim. Biophys. Acta 1217:312-316(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary gland.
    3. "Deficiency of the tetraspanin CD63 associated with kidney pathology but normal lysosomal function."
      Schroder J., Lullmann-Rauch R., Himmerkus N., Pleines I., Nieswandt B., Orinska Z., Koch-Nolte F., Schroder B., Bleich M., Saftig P.
      Mol. Cell. Biol. 29:1083-1094(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
    4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-130.
    5. "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin."
      Doyle E.L., Ridger V., Ferraro F., Turmaine M., Saftig P., Cutler D.F.
      Blood 118:4265-4273(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    6. "The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis."
      van Niel G., Charrin S., Simoes S., Romao M., Rochin L., Saftig P., Marks M.S., Rubinstein E., Raposo G.
      Dev. Cell 21:708-721(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MELANOCYTE DEVELOPMENT, DISRUPTION PHENOTYPE.
    7. "Tetraspanin CD63 promotes vascular endothelial growth factor receptor 2-beta1 integrin complex formation, thereby regulating activation and downstream signaling in endothelial cells in vitro and in vivo."
      Tugues S., Honjo S., Konig C., Padhan N., Kroon J., Gualandi L., Li X., Barkefors I., Thijssen V.L., Griffioen A.W., Claesson-Welsh L.
      J. Biol. Chem. 288:19060-19071(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    8. "The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis."
      Kraft S., Jouvin M.H., Kulkarni N., Kissing S., Morgan E.S., Dvorak A.M., Schroder B., Saftig P., Kinet J.P.
      J. Immunol. 191:2871-2878(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCD63_MOUSE
    AccessioniPrimary (citable) accession number: P41731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3