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P41731

- CD63_MOUSE

UniProt

P41731 - CD63_MOUSE

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Protein

CD63 antigen

Gene
Cd63
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.5 Publications

GO - Molecular functioni

  1. protein binding Source: MGI

GO - Biological processi

  1. cell-matrix adhesion Source: UniProtKB
  2. cell migration Source: UniProtKB
  3. cellular protein localization Source: MGI
  4. endosome to melanosome transport Source: UniProtKB
  5. pigmentation Source: UniProtKB
  6. pigment cell differentiation Source: UniProtKB
  7. pigment granule maturation Source: UniProtKB
  8. positive regulation of integrin-mediated signaling pathway Source: UniProtKB
  9. positive regulation of receptor internalization Source: UniProtKB
  10. protein transport Source: UniProtKB-KW
  11. regulation of rubidium ion transport Source: MGI
  12. regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
CD63 antigen
Alternative name(s):
CD_antigen: CD63
Gene namesi
Name:Cd63
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:99529. Cd63.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosomemultivesicular body By similarity. Melanosome By similarity
Note: Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies By similarity.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1110Cytoplasmic Reviewed prediction
Transmembranei12 – 3221Helical; Reviewed predictionAdd
BLAST
Topological domaini33 – 5119Extracellular Reviewed predictionAdd
BLAST
Transmembranei52 – 7221Helical; Reviewed predictionAdd
BLAST
Topological domaini73 – 819Cytoplasmic Reviewed prediction
Transmembranei82 – 10221Helical; Reviewed predictionAdd
BLAST
Topological domaini103 – 203101Extracellular Reviewed predictionAdd
BLAST
Transmembranei204 – 22421Helical; Reviewed predictionAdd
BLAST
Topological domaini225 – 23814Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. endosome membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. intrinsic component of plasma membrane Source: UniProtKB
  5. late endosome Source: MGI
  6. late endosome membrane Source: UniProtKB
  7. lysosomal membrane Source: UniProtKB
  8. lysosome Source: MGI
  9. melanosome Source: UniProtKB-SubCell
  10. multivesicular body, internal vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mutant mice are viable and fertile, but display impaired water homeostasis, with increased urinary flow, increased water intake, reduced urine osmolality and increased fecal water content. Mice display inclusions in the principal cells in the renal collecting duct (1 Publication). Mutant mice display variable graying of their coat color and a dramatic reduction in the number of melanosomes in the retinal pigment epithelium (1 Publication). According to 1 Publication, mutant mice display a defect in the Selp-dependent attachment of leukocytes to endothelial cells. According to 1 Publication, mast cells from mutant mice show decreased degranulation and decreased release of TNF in response to Ms4a2/FceRI stimulation, but no difference in mast cell degranulation in response to other stimuli and no change in the release of IL6 and leukotriene C4.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 238237CD63 antigenPRO_0000219217Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi130 – 1301N-linked (GlcNAc...)1 Publication
Glycosylationi150 – 1501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi172 – 1721N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP41731.
PaxDbiP41731.
PRIDEiP41731.

PTM databases

PhosphoSiteiP41731.

Expressioni

Tissue specificityi

Ubiquitous. Strongly expressed in kidney. Detected in spleen, bone marrow, peripheral blood mononuclear cells and macrophages.

Gene expression databases

ArrayExpressiP41731.
BgeeiP41731.
CleanExiMM_CD63.
GenevestigatoriP41731.

Interactioni

Subunit structurei

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes By similarity.

Protein-protein interaction databases

IntActiP41731. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP41731.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268658.
HOVERGENiHBG107306.
InParanoidiP41731.
KOiK06497.
OMAiVTQGCGI.
OrthoDBiEOG73BVDM.
TreeFamiTF316345.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41731-1 [UniParc]FASTAAdd to Basket

« Hide

MAVEGGMKCV KFLLYVLLLA FCACAVGLIA IGVAVQVVLK QAITHETTAG    50
SLLPVVIIAV GAFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAV 100
AIAGYVFRDQ VKSEFNKSFQ QQMQNYLKDN KTATILDKLQ KENNCCGASN 150
YTDWENIPGM AKDRVPDSCC INITVGCGND FKESTIHTQG CVETIAIWLR 200
KNILLVAAAA LGIAFVEVLG IIFSCCLVKS IRSGYEVM 238
Length:238
Mass (Da):25,767
Last modified:January 23, 2007 - v2
Checksum:iB57E47B31E13EED8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16432 mRNA. Translation: BAA03904.1.
BC008108 mRNA. Translation: AAH08108.1.
BC012212 mRNA. Translation: AAH12212.1.
BC083161 mRNA. Translation: AAH83161.1.
CCDSiCCDS24297.1.
PIRiS43511.
RefSeqiNP_001036045.1. NM_001042580.1.
NP_001269895.1. NM_001282966.1.
NP_031679.1. NM_007653.3.
UniGeneiMm.371552.
Mm.416079.

Genome annotation databases

EnsembliENSMUST00000026407; ENSMUSP00000026407; ENSMUSG00000025351.
ENSMUST00000105229; ENSMUSP00000100862; ENSMUSG00000025351.
GeneIDi12512.
KEGGimmu:12512.
UCSCiuc007hou.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16432 mRNA. Translation: BAA03904.1 .
BC008108 mRNA. Translation: AAH08108.1 .
BC012212 mRNA. Translation: AAH12212.1 .
BC083161 mRNA. Translation: AAH83161.1 .
CCDSi CCDS24297.1.
PIRi S43511.
RefSeqi NP_001036045.1. NM_001042580.1.
NP_001269895.1. NM_001282966.1.
NP_031679.1. NM_007653.3.
UniGenei Mm.371552.
Mm.416079.

3D structure databases

ProteinModelPortali P41731.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P41731. 2 interactions.

PTM databases

PhosphoSitei P41731.

Proteomic databases

MaxQBi P41731.
PaxDbi P41731.
PRIDEi P41731.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026407 ; ENSMUSP00000026407 ; ENSMUSG00000025351 .
ENSMUST00000105229 ; ENSMUSP00000100862 ; ENSMUSG00000025351 .
GeneIDi 12512.
KEGGi mmu:12512.
UCSCi uc007hou.1. mouse.

Organism-specific databases

CTDi 967.
MGIi MGI:99529. Cd63.

Phylogenomic databases

eggNOGi NOG268658.
HOVERGENi HBG107306.
InParanoidi P41731.
KOi K06497.
OMAi VTQGCGI.
OrthoDBi EOG73BVDM.
TreeFami TF316345.

Miscellaneous databases

NextBioi 281496.
PROi P41731.
SOURCEi Search...

Gene expression databases

ArrayExpressi P41731.
Bgeei P41731.
CleanExi MM_CD63.
Genevestigatori P41731.

Family and domain databases

InterProi IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view ]
Pfami PF00335. Tetraspannin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002419. Tetraspanin. 1 hit.
PRINTSi PR00259. TMFOUR.
SUPFAMi SSF48652. SSF48652. 1 hit.
PROSITEi PS00421. TM4_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages."
    Miyamoto M., Homma M., Hotta H.
    Biochim. Biophys. Acta 1217:312-316(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary gland.
  3. "Deficiency of the tetraspanin CD63 associated with kidney pathology but normal lysosomal function."
    Schroder J., Lullmann-Rauch R., Himmerkus N., Pleines I., Nieswandt B., Orinska Z., Koch-Nolte F., Schroder B., Bleich M., Saftig P.
    Mol. Cell. Biol. 29:1083-1094(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-130.
  5. "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin."
    Doyle E.L., Ridger V., Ferraro F., Turmaine M., Saftig P., Cutler D.F.
    Blood 118:4265-4273(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis."
    van Niel G., Charrin S., Simoes S., Romao M., Rochin L., Saftig P., Marks M.S., Rubinstein E., Raposo G.
    Dev. Cell 21:708-721(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MELANOCYTE DEVELOPMENT, DISRUPTION PHENOTYPE.
  7. "Tetraspanin CD63 promotes vascular endothelial growth factor receptor 2-beta1 integrin complex formation, thereby regulating activation and downstream signaling in endothelial cells in vitro and in vivo."
    Tugues S., Honjo S., Konig C., Padhan N., Kroon J., Gualandi L., Li X., Barkefors I., Thijssen V.L., Griffioen A.W., Claesson-Welsh L.
    J. Biol. Chem. 288:19060-19071(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis."
    Kraft S., Jouvin M.H., Kulkarni N., Kissing S., Morgan E.S., Dvorak A.M., Schroder B., Saftig P., Kinet J.P.
    J. Immunol. 191:2871-2878(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCD63_MOUSE
AccessioniPrimary (citable) accession number: P41731
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi