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Protein

Bud site selection protein 6

Gene

BUD6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Not essential for mitotic growth but is necessary for normal morphogenesis. Involved in the organization and/or function of the actin cytoskeleton.

GO - Molecular functioni

  • actin binding Source: SGD
  • cytoskeletal regulatory protein binding Source: SGD
  • enzyme activator activity Source: SGD

GO - Biological processi

  • actin filament bundle assembly Source: SGD
  • astral microtubule organization Source: SGD
  • bipolar cellular bud site selection Source: SGD
  • budding cell apical bud growth Source: SGD
  • establishment of cell polarity Source: SGD
  • extrachromosomal circular DNA localization involved in cell aging Source: SGD
  • positive regulation of actin cytoskeleton reorganization Source: SGD
  • positive regulation of formin-nucleated actin cable assembly Source: SGD
  • pseudohyphal growth Source: SGD
  • regulation of protein localization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32403-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 6
Alternative name(s):
Actin-interacting protein 3
Gene namesi
Name:BUD6
Synonyms:AIP3
Ordered Locus Names:YLR319C
ORF Names:L8543.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR319C.
SGDiS000004311. BUD6.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
  • polarisome Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Bud site selection protein 6PRO_0000065018Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphothreonineCombined sources
Modified residuei233 – 2331PhosphoserineCombined sources
Modified residuei327 – 3271PhosphoserineCombined sources
Modified residuei342 – 3421PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41697.
PRIDEiP41697.

PTM databases

iPTMnetiP41697.

Interactioni

GO - Molecular functioni

  • actin binding Source: SGD
  • cytoskeletal regulatory protein binding Source: SGD

Protein-protein interaction databases

BioGridi31583. 90 interactions.
DIPiDIP-880N.
IntActiP41697. 5 interactions.
MINTiMINT-408530.

Structurei

Secondary structure

1
788
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi555 – 59440Combined sources
Helixi600 – 67677Combined sources
Helixi701 – 71313Combined sources
Helixi719 – 73315Combined sources
Turni734 – 7374Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OKQX-ray2.04A549-688[»]
3ONXX-ray2.90A/B549-688[»]
4WYBX-ray3.49B/D/F/H/J/L/N/P/R/T/V/Y699-788[»]
ProteinModelPortaliP41697.
SMRiP41697. Positions 555-679, 700-739.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41697.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili573 – 62856Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000001134.
InParanoidiP41697.
OMAiCEERDML.
OrthoDBiEOG092C0X7Y.

Family and domain databases

InterProiIPR005613. AIP3.
IPR022782. AIP3_C.
[Graphical view]
PfamiPF03915. AIP3. 1 hit.
[Graphical view]
SMARTiSM00806. AIP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMAVDDPTY GTPKIKRTAS SSSSIETTVT KLLMSTKHLL QVLTQWSKGT
60 70 80 90 100
TSGRLVSDAY VQLGNDFKVV SKFFMHAKVD MSDVGDVPMA LRRVLEVTLR
110 120 130 140 150
EPPSDETLNK HLPKIREIIV TLLDKLKVKQ AILKNMQQEH RISVKSHHQQ
160 170 180 190 200
NPSFTSNLSL GSEGTREGTP LSSRKSSIVR DQRQSDSVEN SYGEKVNSTS
210 220 230 240 250
TGTPSAQSAE ATLTKPRTNI KQNLKSNNAP NASDDDDALS QLKKGTNLQR
260 270 280 290 300
RASKRYSAYH MAKLTNQSTT EAAAAAGLMT TPSPSMLHLE ETVRKSKLYG
310 320 330 340 350
NNNNDDDRNI NSAENKGKSI DDVSKASPLA KTPLPIENVR ASPRRLSSVV
360 370 380 390 400
TTSPDKAMNG TCPVFLRIGD KTKKCHVQLP TTKNALRLLF IERFAYSPGA
410 420 430 440 450
NSFPDIYIMD PQYGVFYELE ELNLLDIKEG FVIELKLEEN PNNTIKEFID
460 470 480 490 500
TVKMEISNSQ NDIIRHLKEM SFGSAISGKQ TEVLPQPGLE ANKHDLVGQN
510 520 530 540 550
KKDDDKTIKD IQYELGKIKQ VHNINRSNIN ETIFNILRKV DNFKSLSFSA
560 570 580 590 600
KNSSNRMYME KSQTELGDLS DTLLSKVDDL QDVIEIMRKD VAERRSQPAK
610 620 630 640 650
KKLETVSKDL ENAQADVLKL QEFIDTEKPH WKKTWEAELD KVCEEQQFLT
660 670 680 690 700
LQEELILDLK EDLGKALETF DLIKLCCEEQ EKNPSRSKSN PILPIMRPGT
710 720 730 740 750
FNQVREQVMV AVQSLNPDHD SRVEAIDKAE KMWEMERKLK ASNEFDDELE
760 770 780
NFVGNSNLKK SGGFEEVERI RKQKDEANLR AYFGPGFT
Length:788
Mass (Da):88,817
Last modified:November 1, 1995 - v1
Checksum:i4BAE8F541B8BDF1F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti376 – 3761Missing in AAB04948 (PubMed:9247651).Curated
Sequence conflicti384 – 3852NA → KP in AAB04948 (PubMed:9247651).Curated
Sequence conflicti434 – 4341E → A in AAB04948 (PubMed:9247651).Curated
Sequence conflicti441 – 4411P → S in AAB04948 (PubMed:9247651).Curated
Sequence conflicti454 – 4541M → I in AAB04948 (PubMed:9247651).Curated
Sequence conflicti565 – 5684ELGD → SH in AAB04948 (PubMed:9247651).Curated
Sequence conflicti742 – 7421S → T in AAB04948 (PubMed:9247651).Curated
Sequence conflicti764 – 78825FEEVE…GPGFT → LKK in AAB04948 (PubMed:9247651).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38903 Genomic DNA. Translation: AAB04948.1.
U35668 Genomic DNA. Translation: AAA79143.1.
U20618 Genomic DNA. Translation: AAB64514.1.
BK006945 Genomic DNA. Translation: DAA09628.1.
PIRiS53397.
RefSeqiNP_013423.1. NM_001182208.1.

Genome annotation databases

EnsemblFungiiYLR319C; YLR319C; YLR319C.
GeneIDi851029.
KEGGisce:YLR319C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38903 Genomic DNA. Translation: AAB04948.1.
U35668 Genomic DNA. Translation: AAA79143.1.
U20618 Genomic DNA. Translation: AAB64514.1.
BK006945 Genomic DNA. Translation: DAA09628.1.
PIRiS53397.
RefSeqiNP_013423.1. NM_001182208.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OKQX-ray2.04A549-688[»]
3ONXX-ray2.90A/B549-688[»]
4WYBX-ray3.49B/D/F/H/J/L/N/P/R/T/V/Y699-788[»]
ProteinModelPortaliP41697.
SMRiP41697. Positions 555-679, 700-739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31583. 90 interactions.
DIPiDIP-880N.
IntActiP41697. 5 interactions.
MINTiMINT-408530.

PTM databases

iPTMnetiP41697.

Proteomic databases

MaxQBiP41697.
PRIDEiP41697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR319C; YLR319C; YLR319C.
GeneIDi851029.
KEGGisce:YLR319C.

Organism-specific databases

EuPathDBiFungiDB:YLR319C.
SGDiS000004311. BUD6.

Phylogenomic databases

HOGENOMiHOG000001134.
InParanoidiP41697.
OMAiCEERDML.
OrthoDBiEOG092C0X7Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-32403-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP41697.
PROiP41697.

Family and domain databases

InterProiIPR005613. AIP3.
IPR022782. AIP3_C.
[Graphical view]
PfamiPF03915. AIP3. 1 hit.
[Graphical view]
SMARTiSM00806. AIP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUD6_YEAST
AccessioniPrimary (citable) accession number: P41697
Secondary accession number(s): D6VYW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.