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P41695

- BUB1_YEAST

UniProt

P41695 - BUB1_YEAST

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Protein

Checkpoint serine/threonine-protein kinase BUB1

Gene
BUB1, YGR188C, G7542
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei733 – 7331ATP
Active sitei833 – 8331Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi711 – 7199ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein kinase activity Source: SGD
  4. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. centromere complex assembly Source: SGD
  2. mitotic spindle assembly checkpoint Source: SGD
  3. protein localization to kinetochore Source: SGD
  4. protein phosphorylation Source: SGD
  5. sister chromatid biorientation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30878-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Checkpoint serine/threonine-protein kinase BUB1 (EC:2.7.11.1)
Gene namesi
Name:BUB1
Ordered Locus Names:YGR188C
ORF Names:G7542
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR188c.
SGDiS000003420. BUB1.

Subcellular locationi

GO - Cellular componenti

  1. condensed nuclear chromosome, centromeric region Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
  3. kinetochore Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi733 – 7331K → R: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10211021Checkpoint serine/threonine-protein kinase BUB1PRO_0000085676Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41695.
PaxDbiP41695.

Expressioni

Gene expression databases

GenevestigatoriP41695.

Interactioni

Subunit structurei

Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB3P2644911EBI-3816,EBI-3830
CDC20P263092EBI-3816,EBI-4212
MAD1P409575EBI-3816,EBI-10354

Protein-protein interaction databases

BioGridi33440. 473 interactions.
DIPiDIP-2236N.
IntActiP41695. 10 interactions.
MINTiMINT-503980.
STRINGi4932.YGR188C.

Structurei

Secondary structure

1
1021
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi307 – 3093
Beta strandi317 – 3215
Helixi323 – 3264
Beta strandi330 – 3323
Helixi336 – 3438

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I3SX-ray1.90B/D/F315-350[»]
3ESLX-ray1.74A/B29-230[»]
4BL0X-ray1.95B/E289-359[»]
ProteinModelPortaliP41695.
SMRiP41695. Positions 29-230, 303-347, 711-914.

Miscellaneous databases

EvolutionaryTraceiP41695.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 212166BUB1 N-terminalAdd
BLAST
Domaini705 – 1021317Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG317001.
GeneTreeiENSGT00520000055622.
HOGENOMiHOG000248151.
KOiK02178.
OMAiWSYEADY.
OrthoDBiEOG79KPPQ.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR012572. Mad3-like.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41695-1 [UniParc]FASTAAdd to Basket

« Hide

MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND     50
LEDMDDPLDL FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET 100
YRNDPRFLKI WIWYINLFLS NNFHESENTF KYMFNKGIGT KLSLFYEEFS 150
KLLENAQFFL EAKVLLELGA ENNCRPYNRL LRSLSNYEDR LREMNIVENQ 200
NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA NLNSNVGVGK 250
SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ 300
SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ 350
RRGKKHTEDY TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD 400
DDNPSQSTHH KNTQVQVQTT TSILPLKPVV DGNLAHETPV KPSLTSNASR 450
SPTVTAFSKD AINEVFSMFN QHYSTPGALL DGDDTTTSKF NVFENFTQEF 500
TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS TRPEKADYMT 550
PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT 600
QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR 650
AKFLSEISPP LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK 700
TGDLYCIRGE LGEGGYATVY LAESSQGHLR ALKVEKPASV WEYYIMSQVE 750
FRLRKSTILK SIINASALHL FLDESYLVLN YASQGTVLDL INLQREKAID 800
GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM IRLEKPGEPL 850
GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE 900
MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK 950
KEIWGVIFDL LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV 1000
ILSIEEELSH FQYKGKPSRR F 1021
Length:1,021
Mass (Da):117,868
Last modified:October 1, 1996 - v2
Checksum:i6D76FC980775D3F9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti531 – 5311D → V in AAA64894. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L32027 Genomic DNA. Translation: AAA64894.1.
Z72973 Genomic DNA. Translation: CAA97214.1.
X99074 Genomic DNA. Translation: CAA67524.1.
BK006941 Genomic DNA. Translation: DAA08282.1.
PIRiS64506.
RefSeqiNP_011704.3. NM_001181317.3.

Genome annotation databases

EnsemblFungiiYGR188C; YGR188C; YGR188C.
GeneIDi853100.
KEGGisce:YGR188C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L32027 Genomic DNA. Translation: AAA64894.1 .
Z72973 Genomic DNA. Translation: CAA97214.1 .
X99074 Genomic DNA. Translation: CAA67524.1 .
BK006941 Genomic DNA. Translation: DAA08282.1 .
PIRi S64506.
RefSeqi NP_011704.3. NM_001181317.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I3S X-ray 1.90 B/D/F 315-350 [» ]
3ESL X-ray 1.74 A/B 29-230 [» ]
4BL0 X-ray 1.95 B/E 289-359 [» ]
ProteinModelPortali P41695.
SMRi P41695. Positions 29-230, 303-347, 711-914.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33440. 473 interactions.
DIPi DIP-2236N.
IntActi P41695. 10 interactions.
MINTi MINT-503980.
STRINGi 4932.YGR188C.

Proteomic databases

MaxQBi P41695.
PaxDbi P41695.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR188C ; YGR188C ; YGR188C .
GeneIDi 853100.
KEGGi sce:YGR188C.

Organism-specific databases

CYGDi YGR188c.
SGDi S000003420. BUB1.

Phylogenomic databases

eggNOGi NOG317001.
GeneTreei ENSGT00520000055622.
HOGENOMi HOG000248151.
KOi K02178.
OMAi WSYEADY.
OrthoDBi EOG79KPPQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30878-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

EvolutionaryTracei P41695.
NextBioi 973099.
PROi P41695.

Gene expression databases

Genevestigatori P41695.

Family and domain databases

InterProi IPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR012572. Mad3-like.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR14030. PTHR14030. 1 hit.
Pfami PF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00777. Mad3_BUB1_I. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase."
    Roberts B.T., Farr K.A., Hoyt M.A.
    Mol. Cell. Biol. 14:8282-8291(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
    Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
    Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function."
    Brady D.M., Hardwick K.G.
    Curr. Biol. 10:675-678(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH MAD1 AND BUB3.
  6. "Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint."
    Kitagawa K., Abdulle R., Bansal P.K., Cagney G., Fields S., Hieter P.
    Mol. Cell 11:1201-1213(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SKP1.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBUB1_YEAST
AccessioniPrimary (citable) accession number: P41695
Secondary accession number(s): D6VUX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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