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Protein

Checkpoint serine/threonine-protein kinase BUB1

Gene

BUB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei733ATP1
Active sitei833Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi711 – 719ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • centromere complex assembly Source: SGD
  • macroautophagy Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • protein localization to kinetochore Source: SGD
  • protein phosphorylation Source: SGD
  • sister chromatid biorientation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30878-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Checkpoint serine/threonine-protein kinase BUB1 (EC:2.7.11.1)
Gene namesi
Name:BUB1
Ordered Locus Names:YGR188C
ORF Names:G7542
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR188C.
SGDiS000003420. BUB1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
  • kinetochore Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi733K → R: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856761 – 1021Checkpoint serine/threonine-protein kinase BUB1Add BLAST1021

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41695.
PRIDEiP41695.

PTM databases

iPTMnetiP41695.

Interactioni

Subunit structurei

Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB3P2644911EBI-3816,EBI-3830
CDC20P263092EBI-3816,EBI-4212
MAD1P409575EBI-3816,EBI-10354

Protein-protein interaction databases

BioGridi33440. 474 interactors.
DIPiDIP-2236N.
IntActiP41695. 10 interactors.
MINTiMINT-503980.

Structurei

Secondary structure

11021
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi307 – 309Combined sources3
Beta strandi317 – 321Combined sources5
Helixi323 – 326Combined sources4
Beta strandi330 – 332Combined sources3
Helixi336 – 343Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I3SX-ray1.90B/D/F315-350[»]
3ESLX-ray1.74A/B29-230[»]
4BL0X-ray1.95B/E289-359[»]
ProteinModelPortaliP41695.
SMRiP41695.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41695.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 212BUB1 N-terminalPROSITE-ProRule annotationAdd BLAST166
Domaini705 – 1021Protein kinasePROSITE-ProRule annotationAdd BLAST317

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.PROSITE-ProRule annotation
Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00520000055622.
HOGENOMiHOG000248151.
InParanoidiP41695.
KOiK02178.
OMAiWSYEADY.
OrthoDBiEOG092C0DAL.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3/Bub1_I.
IPR012572. Mad3/Bub1_II.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41695-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND
60 70 80 90 100
LEDMDDPLDL FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET
110 120 130 140 150
YRNDPRFLKI WIWYINLFLS NNFHESENTF KYMFNKGIGT KLSLFYEEFS
160 170 180 190 200
KLLENAQFFL EAKVLLELGA ENNCRPYNRL LRSLSNYEDR LREMNIVENQ
210 220 230 240 250
NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA NLNSNVGVGK
260 270 280 290 300
SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ
310 320 330 340 350
SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ
360 370 380 390 400
RRGKKHTEDY TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD
410 420 430 440 450
DDNPSQSTHH KNTQVQVQTT TSILPLKPVV DGNLAHETPV KPSLTSNASR
460 470 480 490 500
SPTVTAFSKD AINEVFSMFN QHYSTPGALL DGDDTTTSKF NVFENFTQEF
510 520 530 540 550
TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS TRPEKADYMT
560 570 580 590 600
PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT
610 620 630 640 650
QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR
660 670 680 690 700
AKFLSEISPP LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK
710 720 730 740 750
TGDLYCIRGE LGEGGYATVY LAESSQGHLR ALKVEKPASV WEYYIMSQVE
760 770 780 790 800
FRLRKSTILK SIINASALHL FLDESYLVLN YASQGTVLDL INLQREKAID
810 820 830 840 850
GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM IRLEKPGEPL
860 870 880 890 900
GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE
910 920 930 940 950
MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK
960 970 980 990 1000
KEIWGVIFDL LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV
1010 1020
ILSIEEELSH FQYKGKPSRR F
Length:1,021
Mass (Da):117,868
Last modified:October 1, 1996 - v2
Checksum:i6D76FC980775D3F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531D → V in AAA64894 (PubMed:7969164).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32027 Genomic DNA. Translation: AAA64894.1.
Z72973 Genomic DNA. Translation: CAA97214.1.
X99074 Genomic DNA. Translation: CAA67524.1.
BK006941 Genomic DNA. Translation: DAA08282.1.
PIRiS64506.
RefSeqiNP_011704.3. NM_001181317.3.

Genome annotation databases

EnsemblFungiiYGR188C; YGR188C; YGR188C.
GeneIDi853100.
KEGGisce:YGR188C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32027 Genomic DNA. Translation: AAA64894.1.
Z72973 Genomic DNA. Translation: CAA97214.1.
X99074 Genomic DNA. Translation: CAA67524.1.
BK006941 Genomic DNA. Translation: DAA08282.1.
PIRiS64506.
RefSeqiNP_011704.3. NM_001181317.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I3SX-ray1.90B/D/F315-350[»]
3ESLX-ray1.74A/B29-230[»]
4BL0X-ray1.95B/E289-359[»]
ProteinModelPortaliP41695.
SMRiP41695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33440. 474 interactors.
DIPiDIP-2236N.
IntActiP41695. 10 interactors.
MINTiMINT-503980.

PTM databases

iPTMnetiP41695.

Proteomic databases

MaxQBiP41695.
PRIDEiP41695.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR188C; YGR188C; YGR188C.
GeneIDi853100.
KEGGisce:YGR188C.

Organism-specific databases

EuPathDBiFungiDB:YGR188C.
SGDiS000003420. BUB1.

Phylogenomic databases

GeneTreeiENSGT00520000055622.
HOGENOMiHOG000248151.
InParanoidiP41695.
KOiK02178.
OMAiWSYEADY.
OrthoDBiEOG092C0DAL.

Enzyme and pathway databases

BioCyciYEAST:G3O-30878-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Miscellaneous databases

EvolutionaryTraceiP41695.
PROiP41695.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3/Bub1_I.
IPR012572. Mad3/Bub1_II.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUB1_YEAST
AccessioniPrimary (citable) accession number: P41695
Secondary accession number(s): D6VUX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.