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P41695

- BUB1_YEAST

UniProt

P41695 - BUB1_YEAST

Protein

Checkpoint serine/threonine-protein kinase BUB1

Gene

BUB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei733 – 7331ATP
    Active sitei833 – 8331Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi711 – 7199ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein kinase activity Source: SGD
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. centromere complex assembly Source: SGD
    2. mitotic spindle assembly checkpoint Source: SGD
    3. protein localization to kinetochore Source: SGD
    4. protein phosphorylation Source: SGD
    5. sister chromatid biorientation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30878-MONOMER.
    BRENDAi2.7.11.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Checkpoint serine/threonine-protein kinase BUB1 (EC:2.7.11.1)
    Gene namesi
    Name:BUB1
    Ordered Locus Names:YGR188C
    ORF Names:G7542
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR188c.
    SGDiS000003420. BUB1.

    Subcellular locationi

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. kinetochore Source: SGD
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi733 – 7331K → R: Loss of activity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10211021Checkpoint serine/threonine-protein kinase BUB1PRO_0000085676Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP41695.
    PaxDbiP41695.

    Expressioni

    Gene expression databases

    GenevestigatoriP41695.

    Interactioni

    Subunit structurei

    Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUB3P2644911EBI-3816,EBI-3830
    CDC20P263092EBI-3816,EBI-4212
    MAD1P409575EBI-3816,EBI-10354

    Protein-protein interaction databases

    BioGridi33440. 473 interactions.
    DIPiDIP-2236N.
    IntActiP41695. 10 interactions.
    MINTiMINT-503980.
    STRINGi4932.YGR188C.

    Structurei

    Secondary structure

    1
    1021
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi307 – 3093
    Beta strandi317 – 3215
    Helixi323 – 3264
    Beta strandi330 – 3323
    Helixi336 – 3438

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2I3SX-ray1.90B/D/F315-350[»]
    3ESLX-ray1.74A/B29-230[»]
    4BL0X-ray1.95B/E289-359[»]
    ProteinModelPortaliP41695.
    SMRiP41695. Positions 29-230, 303-347, 711-914.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP41695.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 212166BUB1 N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini705 – 1021317Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.PROSITE-ProRule annotation
    Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG317001.
    GeneTreeiENSGT00520000055622.
    HOGENOMiHOG000248151.
    KOiK02178.
    OMAiWSYEADY.
    OrthoDBiEOG79KPPQ.

    Family and domain databases

    InterProiIPR015661. Bub1/Mad3.
    IPR011009. Kinase-like_dom.
    IPR012572. Mad3-like.
    IPR013212. Mad3_BUB1_I.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR14030. PTHR14030. 1 hit.
    PfamiPF08311. Mad3_BUB1_I. 1 hit.
    PF08171. Mad3_BUB1_II. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00777. Mad3_BUB1_I. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51489. BUB1_N. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41695-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND     50
    LEDMDDPLDL FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET 100
    YRNDPRFLKI WIWYINLFLS NNFHESENTF KYMFNKGIGT KLSLFYEEFS 150
    KLLENAQFFL EAKVLLELGA ENNCRPYNRL LRSLSNYEDR LREMNIVENQ 200
    NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA NLNSNVGVGK 250
    SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ 300
    SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ 350
    RRGKKHTEDY TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD 400
    DDNPSQSTHH KNTQVQVQTT TSILPLKPVV DGNLAHETPV KPSLTSNASR 450
    SPTVTAFSKD AINEVFSMFN QHYSTPGALL DGDDTTTSKF NVFENFTQEF 500
    TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS TRPEKADYMT 550
    PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT 600
    QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR 650
    AKFLSEISPP LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK 700
    TGDLYCIRGE LGEGGYATVY LAESSQGHLR ALKVEKPASV WEYYIMSQVE 750
    FRLRKSTILK SIINASALHL FLDESYLVLN YASQGTVLDL INLQREKAID 800
    GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM IRLEKPGEPL 850
    GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE 900
    MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK 950
    KEIWGVIFDL LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV 1000
    ILSIEEELSH FQYKGKPSRR F 1021
    Length:1,021
    Mass (Da):117,868
    Last modified:October 1, 1996 - v2
    Checksum:i6D76FC980775D3F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti531 – 5311D → V in AAA64894. (PubMed:7969164)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32027 Genomic DNA. Translation: AAA64894.1.
    Z72973 Genomic DNA. Translation: CAA97214.1.
    X99074 Genomic DNA. Translation: CAA67524.1.
    BK006941 Genomic DNA. Translation: DAA08282.1.
    PIRiS64506.
    RefSeqiNP_011704.3. NM_001181317.3.

    Genome annotation databases

    EnsemblFungiiYGR188C; YGR188C; YGR188C.
    GeneIDi853100.
    KEGGisce:YGR188C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32027 Genomic DNA. Translation: AAA64894.1 .
    Z72973 Genomic DNA. Translation: CAA97214.1 .
    X99074 Genomic DNA. Translation: CAA67524.1 .
    BK006941 Genomic DNA. Translation: DAA08282.1 .
    PIRi S64506.
    RefSeqi NP_011704.3. NM_001181317.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2I3S X-ray 1.90 B/D/F 315-350 [» ]
    3ESL X-ray 1.74 A/B 29-230 [» ]
    4BL0 X-ray 1.95 B/E 289-359 [» ]
    ProteinModelPortali P41695.
    SMRi P41695. Positions 29-230, 303-347, 711-914.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33440. 473 interactions.
    DIPi DIP-2236N.
    IntActi P41695. 10 interactions.
    MINTi MINT-503980.
    STRINGi 4932.YGR188C.

    Proteomic databases

    MaxQBi P41695.
    PaxDbi P41695.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR188C ; YGR188C ; YGR188C .
    GeneIDi 853100.
    KEGGi sce:YGR188C.

    Organism-specific databases

    CYGDi YGR188c.
    SGDi S000003420. BUB1.

    Phylogenomic databases

    eggNOGi NOG317001.
    GeneTreei ENSGT00520000055622.
    HOGENOMi HOG000248151.
    KOi K02178.
    OMAi WSYEADY.
    OrthoDBi EOG79KPPQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30878-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    EvolutionaryTracei P41695.
    NextBioi 973099.
    PROi P41695.

    Gene expression databases

    Genevestigatori P41695.

    Family and domain databases

    InterProi IPR015661. Bub1/Mad3.
    IPR011009. Kinase-like_dom.
    IPR012572. Mad3-like.
    IPR013212. Mad3_BUB1_I.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR14030. PTHR14030. 1 hit.
    Pfami PF08311. Mad3_BUB1_I. 1 hit.
    PF08171. Mad3_BUB1_II. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00777. Mad3_BUB1_I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51489. BUB1_N. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase."
      Roberts B.T., Farr K.A., Hoyt M.A.
      Mol. Cell. Biol. 14:8282-8291(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
      Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
      Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function."
      Brady D.M., Hardwick K.G.
      Curr. Biol. 10:675-678(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH MAD1 AND BUB3.
    6. "Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint."
      Kitagawa K., Abdulle R., Bansal P.K., Cagney G., Fields S., Hieter P.
      Mol. Cell 11:1201-1213(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SKP1.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUB1_YEAST
    AccessioniPrimary (citable) accession number: P41695
    Secondary accession number(s): D6VUX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 414 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3