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P41695 (BUB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Checkpoint serine/threonine-protein kinase BUB1

EC=2.7.11.1
Gene names
Name:BUB1
Ordered Locus Names:YGR188C
ORF Names:G7542
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1021 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Part of complex consisting of MAD1, BUB1 and BUB3 after activation of spindle checkpoint. Ref.5

Subcellular location

Nucleus.

Post-translational modification

Autophosphorylated.

Miscellaneous

Present with 414 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

Contains 1 BUB1 N-terminal domain.

Contains 1 protein kinase domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10211021Checkpoint serine/threonine-protein kinase BUB1
PRO_0000085676

Regions

Domain47 – 212166BUB1 N-terminal
Domain705 – 1021317Protein kinase
Nucleotide binding711 – 7199ATP By similarity

Sites

Active site8331Proton acceptor By similarity
Binding site7331ATP

Experimental info

Mutagenesis7331K → R: Loss of activity.
Sequence conflict5311D → V in AAA64894. Ref.1

Secondary structure

........... 1021
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P41695 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 6D76FC980775D3F9

FASTA1,021117,868
        10         20         30         40         50         60 
MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND LEDMDDPLDL 

        70         80         90        100        110        120 
FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET YRNDPRFLKI WIWYINLFLS 

       130        140        150        160        170        180 
NNFHESENTF KYMFNKGIGT KLSLFYEEFS KLLENAQFFL EAKVLLELGA ENNCRPYNRL 

       190        200        210        220        230        240 
LRSLSNYEDR LREMNIVENQ NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA 

       250        260        270        280        290        300 
NLNSNVGVGK SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ 

       310        320        330        340        350        360 
SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ RRGKKHTEDY 

       370        380        390        400        410        420 
TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD DDNPSQSTHH KNTQVQVQTT 

       430        440        450        460        470        480 
TSILPLKPVV DGNLAHETPV KPSLTSNASR SPTVTAFSKD AINEVFSMFN QHYSTPGALL 

       490        500        510        520        530        540 
DGDDTTTSKF NVFENFTQEF TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS 

       550        560        570        580        590        600 
TRPEKADYMT PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT 

       610        620        630        640        650        660 
QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR AKFLSEISPP 

       670        680        690        700        710        720 
LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK TGDLYCIRGE LGEGGYATVY 

       730        740        750        760        770        780 
LAESSQGHLR ALKVEKPASV WEYYIMSQVE FRLRKSTILK SIINASALHL FLDESYLVLN 

       790        800        810        820        830        840 
YASQGTVLDL INLQREKAID GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM 

       850        860        870        880        890        900 
IRLEKPGEPL GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE 

       910        920        930        940        950        960 
MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK KEIWGVIFDL 

       970        980        990       1000       1010       1020 
LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV ILSIEEELSH FQYKGKPSRR 


F 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase."
Roberts B.T., Farr K.A., Hoyt M.A.
Mol. Cell. Biol. 14:8282-8291(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function."
Brady D.M., Hardwick K.G.
Curr. Biol. 10:675-678(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH MAD1 AND BUB3.
[6]"Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint."
Kitagawa K., Abdulle R., Bansal P.K., Cagney G., Fields S., Hieter P.
Mol. Cell 11:1201-1213(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SKP1.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L32027 Genomic DNA. Translation: AAA64894.1.
Z72973 Genomic DNA. Translation: CAA97214.1.
X99074 Genomic DNA. Translation: CAA67524.1.
BK006941 Genomic DNA. Translation: DAA08282.1.
PIRS64506.
RefSeqNP_011704.3. NM_001181317.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2I3SX-ray1.90B/D/F315-350[»]
3ESLX-ray1.74A/B29-230[»]
4BL0X-ray1.95B/E289-359[»]
ProteinModelPortalP41695.
SMRP41695. Positions 29-230, 303-347, 639-1000.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33440. 471 interactions.
DIPDIP-2236N.
IntActP41695. 10 interactions.
MINTMINT-503980.
STRING4932.YGR188C.

Proteomic databases

PaxDbP41695.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR188C; YGR188C; YGR188C.
GeneID853100.
KEGGsce:YGR188C.

Organism-specific databases

CYGDYGR188c.
SGDS000003420. BUB1.

Phylogenomic databases

eggNOGNOG317001.
GeneTreeENSGT00520000055622.
HOGENOMHOG000248151.
KOK02178.
OMAWSYEADY.
OrthoDBEOG79KPPQ.

Enzyme and pathway databases

BioCycYEAST:G3O-30878-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP41695.

Family and domain databases

InterProIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR012572. Mad3-like.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR14030. PTHR14030. 1 hit.
PfamPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP41695.
NextBio973099.
PROP41695.

Entry information

Entry nameBUB1_YEAST
AccessionPrimary (citable) accession number: P41695
Secondary accession number(s): D6VUX1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references