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Protein

Chitinase

Gene

CHIA

Organism
Autographa californica nuclear polyhedrosis virus (AcMNPV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in host liquefaction to facilitate horizontal transmission of the virus by hydrolyzing beta-chitin and by regulating the cysteine protease VCATH. Localized in the host reticulum endoplasmic via its KDEL motif, interacts with and thus prevents VCATH secretion before host cell lysis occurs.3 Publications

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei305Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase (EC:3.2.1.14)
Gene namesi
Name:CHIA
ORF Names:ORF126
OrganismiAutographa californica nuclear polyhedrosis virus (AcMNPV)
Taxonomic identifieri46015 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageBaculoviridaeAlphabaculovirus
Virus hostiLepidoptera (butterflies and moths) [TaxID: 7088]
Proteomesi
  • UP000008292 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001195018 – 551ChitinaseAdd BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi173N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi444N-linked (GlcNAc...); by hostSequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Interacts with host VCATH.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP41684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149Chitin binding domain (CBD)1 PublicationAdd BLAST149

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi548 – 551Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01183.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
[Graphical view]
PfamiPF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYKLLNVLW LVAVSNAIPG TPVIDWADRN YALVEINYEA TAYENLIKPK
60 70 80 90 100
EQVDVQVSWN VWNGDIGDIA YVLFDEQQVW KGDAESKRAT IKVLVSGQFN
110 120 130 140 150
MRVKLCNEDG CSVSDPVLVK VADTDGGHLA PLEYTWLENN KPGRREDKIV
160 170 180 190 200
AAYFVEWGVY GRNFPVDKVP LPNLSHLLYG FIPICGGDGI NDALKTIPGS
210 220 230 240 250
FESLQRSCKG REDFKVAIHD PWAAVQKPQK GVSAWNEPYK GNFGQLMAAK
260 270 280 290 300
LANPHLKILP SIGGWTLSDP FYFMHDVEKR NVFVDSVKEF LQVWKFFDGV
310 320 330 340 350
DIDWEFPGGK GANPSLGDAD GDAKTYILLL EELRAMLDDL EAQTGRVYEL
360 370 380 390 400
TSAISAGYDK IAVVNYAEAQ KSLGKIFLMS YDFKGAWSNT DLGYQTTVYA
410 420 430 440 450
PSWNSEELYT THYAVDALLK QGVDPNKIIV GVAMYGRGWT GVTNYTNDNY
460 470 480 490 500
FSGTGNGPGS GTWEDGVVDY RQIQKDLNNY VYTFDSAAQA SYVFDKSKGD
510 520 530 540 550
LISFDSVDSV LGKVKYVDRN KLGGLFAWEI DADNGDLLNA INAQFKPKDE

L
Length:551
Mass (Da):61,368
Last modified:November 1, 1995 - v1
Checksum:i4DDAAD187873BBA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22858 Genomic DNA. Translation: AAA66756.1.
PIRiG72865.
RefSeqiNP_054156.1. NC_001623.1.

Genome annotation databases

GeneIDi1403959.
KEGGivg:1403959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22858 Genomic DNA. Translation: AAA66756.1.
PIRiG72865.
RefSeqiNP_054156.1. NC_001623.1.

3D structure databases

ProteinModelPortaliP41684.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1403959.
KEGGivg:1403959.

Phylogenomic databases

KOiK01183.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
[Graphical view]
PfamiPF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIT_NPVAC
AccessioniPrimary (citable) accession number: P41684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.