Reviewed, UniProtKB/Swiss-Prot P41682 (ADH7_RAT)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase class 4 mu/sigma chain
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase class IV mu/sigma chain

Gene names

Name:

Adh7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Preferentially expressed in stomach.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW retinol metabolic process Inferred from direct assay. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NAD or NADH binding Inferred from direct assay. Source: RGD alcohol dehydrogenase (NAD) activity Inferred from direct assay. Source: RGD ethanol binding Inferred from direct assay. Source: RGD protein homodimerization activity Inferred from direct assay. Source: RGD retinol binding Inferred from direct assay. Source: RGD zinc ion binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Alcohol dehydrogenase class 4 mu/sigma chain		PRO_0000160696
Regions
	Nucleotide binding	199 – 204	6	NAD By similarity
	Nucleotide binding	292 – 294	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic By similarity
	Metal binding	68	1	Zinc 1; catalytic By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	104	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 1; catalytic By similarity
	Binding site	223	1	NAD By similarity
	Binding site	228	1	NAD By similarity
	Binding site	369	1	NAD By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Probable
Experimental info
	Sequence conflict	1 – 4	4	MDTA → SNRV AA sequence Ref.1
	Sequence conflict	109	1	G → E in CAA67297. Ref.1
	Sequence conflict	208	1	V → I AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41682-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: DD745E7FCB01E452	FASTA	374	40,105
	10 20 30 40 50 60 MDTAGKVIKC KAAVLWGTNQ PFSIEDIEVA PPKAKEVRVK ILATGICGTD DHVIKGTMVS 70 80 90 100 110 120 KFPVIVGHEA VGIVESVGEE VTTVRPGDKV IPLFLPQCRE CNPCRNPEGN LCIRSDLTGR 130 140 150 160 170 180 GVLADGTTRF TCKGKPVQHF MNTSTFTEYT VLDESSVAKI DAEAPPEKAC LIGCGFSTGY 190 200 210 220 230 240 GAAVKTAKVS PGSTCAVFGL GGVGLSVVMG CKAAGASRII GIDINKDKFQ KALDVGATEC 250 260 270 280 290 300 INPRDFTKPI SEVLSDMTGN TVQYTFEVIG RLETMVDALS SCHMNYGTSV VVGAPPSAKM 310 320 330 340 350 360 LSYDPMLLFT GRTWKGCVFG GWKSRDDVPK LVTEFLEKKF DLGQLITHTL PFHNISEGFE 370 LLYSGQSIRT VLTF

References

[1]	"Mammalian class IV alcohol dehydrogenase (stomach alcohol dehydrogenase): structure, origin, and correlation with enzymology." Pares X., Cederlund E., Moreno A., Hjelmqvist L., Farres J., Joernvall H. Proc. Natl. Acad. Sci. U.S.A. 91:1893-1897(1994) [PubMed: 8127901] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Stomach.
[2]	Crosas-Navarro B., Farres J., Pares X. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98746 mRNA. Translation: CAA67297.1.
IPI	IPI00324743.
PIR	A53142.
RefSeq	NP_599156.1.
UniGene	Rn.42935
3D structure databases
SMR	P41682. Positions 3-374.
ModBase	Search...
Protein-protein interaction databases
STRING	P41682.
Genome annotation databases
Ensembl	ENSRNOT00000015870; ENSRNOP00000015870; ENSRNOG00000032959; Rattus norvegicus. [Genome view]
GeneID	171178.
KEGG	rno:171178.
UCSC	NM_134329. rat.
Organism-specific databases
CTD	171178.
RGD	621638. Adh7.
Phylogenomic databases
eggNOG	roNOG10188.
HOVERGEN	P41682.
InParanoid	P41682.
PhylomeDB	P41682.
Enzyme and pathway databases
BRENDA	1.1.1.1. 248.
Gene expression databases
ArrayExpress	P41682.
Genevestigator	P41682.
GermOnline	ENSRNOG00000032959. Rattus norvegicus.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622023.

Entry information

Entry name

ADH7_RAT

Accession

Primary (citable) accession number: P41682
Secondary accession number(s): O55146

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1999
Last modified:	January 19, 2010
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)