Reviewed, UniProtKB/Swiss-Prot P41636 (4CL_PINTA)

Names and origin

Protein names

Recommended name:
    4-coumarate--CoA ligase
      Short name=4CL
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase

Gene names

Name:

4CL

Organism

Pinus taeda (Loblolly pine)

Taxonomic identifier

3352 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Pinus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 537	537	4-coumarate--CoA ligase		PRO_0000193035
Natural variations
	Natural variant	422	1	E → G

Sequences

	Sequence		Length	Mass (Da)
	P41636-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6686EB2E84C7DC43	FASTA	537	58,591
	10 20 30 40 50 60 MANGIKKVEH LYRSKLPDIE ISDHLPLHSY CFERVAEFAD RPCLIDGATD RTYCFSEVEL 70 80 90 100 110 120 ISRKVAAGLA KLGLQQGQVV MLLLPNCIEF AFVFMGASVR GAIVTTANPF YKPGEIAKQA 130 140 150 160 170 180 KAAGARIIVT LAAYVEKLAD LQSHDVLVIT IDDAPKEGCQ HISVLTEADE TQCPAVKIHP 190 200 210 220 230 240 DDVVALPYSS GTTGLPKGVM LTHKGLVSSV AQQVDGENPN LYFHSDDVIL CVLPLFHIYS 250 260 270 280 290 300 LNSVLLCALR AGAATLIMQK FNLTTCLELI QKYKVTVAPI VPPIVLDITK SPIVSQYDVS 310 320 330 340 350 360 SVRIIMSGAA PLGKELEDAL RERFPKAIFG QGYGMTEAGP VLAMNLAFAK NPFPVKSGSC 370 380 390 400 410 420 GTVVRNAQIK ILDTETGESL PHNQAGEICI RGPEIMKGYI NDPESTAATI DEEGWLHTGD 430 440 450 460 470 480 VEYIDDDEEI FIVDRVKEII KYKGFQVAPA ELEALLVAHP SIADAAVVPQ KHEEAGEVPV 490 500 510 520 530 AFVVKSSEIS EQEIKEFVAK QVIFYKKIHR VYFVDAIPKS PSGKILRKDL RSRLAAK

References

[1]	Voo K.S. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: L.. Tissue: Xylem.
[2]	"Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood." Zhang X.H., Chiang V.L. Plant Physiol. 113:65-74(1997) [PubMed: 9008388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Needle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12012 mRNA. Translation: AAA92668.1. U12013 mRNA. Translation: AAA92669.1. U39404 Genomic DNA. Translation: AAB42382.1. U39405 Genomic DNA. Translation: AAB42383.1.
PIR	T09710. T09755.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.2.1.12. 297.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL_PINTA

Accession

Primary (citable) accession number: P41636

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)