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Protein

Dopamine receptor 1

Gene

Dop1R1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Might be involved in the processing of visual information and/or visual learning. Important for Pavlovian conditioning: required in the mushroom body as a receptor conveying unconditional stimuli information, has a role in memory formation for aversive and appetitive learning. Sleep-deprivation-induced impairments in learning can be partially explained through alterations in dopamine signaling, Dop1R1 expression levels are reduced; sleep may have a role in restoring dopamine homeostasis.5 Publications

Miscellaneous

Potency of neurotransmitter agonists in stimulating cAMP production and the lack of stimulation by other transmitters and metabolites suggests this is a D1-like receptor. Low homology to vertebrate D1 receptors suggests this may be a progenitor of the D1 receptor subfamily.

GO - Molecular functioni

  • dopamine neurotransmitter receptor activity Source: FlyBase
  • dopamine neurotransmitter receptor activity, coupled via Gs Source: FlyBase
  • G-protein coupled receptor activity Source: FlyBase
  • histamine receptor activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-activating dopamine receptor signaling pathway Source: FlyBase
  • associative learning Source: UniProtKB
  • cellular response to sucrose stimulus Source: FlyBase
  • dopamine receptor signaling pathway Source: FlyBase
  • learning Source: FlyBase
  • memory Source: UniProtKB
  • olfactory learning Source: FlyBase
  • regulation of olfactory learning Source: FlyBase
  • regulation of presynaptic cytosolic calcium ion concentration Source: FlyBase
  • response to odorant Source: FlyBase
  • response to starvation Source: FlyBase
  • response to sucrose Source: FlyBase
  • thermosensory behavior Source: FlyBase
  • thermotaxis Source: FlyBase
  • visual learning Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-DME-390651. Dopamine receptors.
R-DME-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Dopamine receptor 1
Short name:
D-DOP1
Short name:
DmDop1
Short name:
dDA1
Alternative name(s):
Dopamine 1-like receptor 1
Gene namesi
Name:Dop1R1
Synonyms:DopR, DopR1, DopR35EF
ORF Names:CG9652
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0011582. Dop1R1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 142ExtracellularSequence analysisAdd BLAST123
Transmembranei143 – 169Helical; Name=1Sequence analysisAdd BLAST27
Topological domaini170 – 179CytoplasmicSequence analysis10
Transmembranei180 – 206Helical; Name=2Sequence analysisAdd BLAST27
Topological domaini207 – 216ExtracellularSequence analysis10
Transmembranei217 – 239Helical; Name=3Sequence analysisAdd BLAST23
Topological domaini240 – 258CytoplasmicSequence analysisAdd BLAST19
Transmembranei259 – 279Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini280 – 310ExtracellularSequence analysisAdd BLAST31
Transmembranei311 – 331Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini332 – 391CytoplasmicSequence analysisAdd BLAST60
Transmembranei392 – 412Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini413 – 427ExtracellularSequence analysisAdd BLAST15
Transmembranei428 – 450Helical; Name=7Sequence analysisAdd BLAST23
Topological domaini451 – 511CytoplasmicSequence analysisAdd BLAST61

GO - Cellular componenti

  • integral component of membrane Source: FlyBase
  • integral component of plasma membrane Source: FlyBase
  • presynapse Source: GOC

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001271820 – 511Dopamine receptor 1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi216 ↔ 302PROSITE-ProRule annotation
Lipidationi468S-palmitoyl cysteineSequence analysis1
Lipidationi469S-palmitoyl cysteineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP41596.
PRIDEiP41596.

Expressioni

Tissue specificityi

Expressed in the larval and adult CNS in structures that mediate higher-order brain functions such as learning, memory and motor control: in the mushroom body neuropil and four unpaired neurons in each thoracic segment. The adult CNS has intense expression in the central complex, moderate expression in several neurosecretory cells, and weak expression in two unpaired neurons in the mesothoracic neuromere. Also seen in the somata of the optic lobes.3 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0011582.
ExpressionAtlasiP41596. differential.
GenevisibleiP41596. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0303554.

Structurei

3D structure databases

ProteinModelPortaliP41596.
SMRiP41596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00890000139331.
InParanoidiP41596.
KOiK04148.
OrthoDBiEOG091G06VI.
PhylomeDBiP41596.

Family and domain databases

InterProiView protein in InterPro
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P41596-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYTPHPFGFL IILVPMTNAM RAIAAIAAGV GSVAATVATS TTSSISSSTT
60 70 80 90 100
IINTSSATTI GGNHTSGSTG FSTNSTLLDA DHLPLQLTTA KVDLDIEIDI
110 120 130 140 150
QLLTNGYDGT TLTSFYNESS WTNASEMDTI VGEEPEPLSL VSIVVVGIFL
160 170 180 190 200
SVLIFLSVAG NILVCLAIYT ERSLRRIGNL FLASLAIADL FVASLVMTFA
210 220 230 240 250
GVNDLLGYWI FGAQFCDTWV AFDVMCSTAS ILNLCAISMD RYIHIKDPLR
260 270 280 290 300
YGRWVTRRVA VITIAAIWLL AAFVSFVPIS LGIHRPDQPL IFEDNGKKYP
310 320 330 340 350
TCALDLTPTY AVVSSCISFY FPCVVMIGIY CRLYCYAQKH VKSIKAVTRP
360 370 380 390 400
GEVAEKQRYK SIRRPKNQPK KFKVRNLHTH SSPYHVSDHK AAVTVGVIMG
410 420 430 440 450
VFLICWVPFF CVNITAAFCK TCIGGQTFKI LTWLGYSNSA FNPIIYSIFN
460 470 480 490 500
KEFRDAFKRI LTMRNPWCCA QDVGNIHPRN SDRFITDYAA KNVVVMNSGR
510
SSAELEQVSA I
Length:511
Mass (Da):56,170
Last modified:November 23, 2004 - v2
Checksum:iF044C00EDBF14749
GO
Isoform B (identifier: P41596-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     133-147: Missing.

Note: No experimental confirmation available.
Show »
Length:496
Mass (Da):54,621
Checksum:i5D4D70DF42D627F1
GO
Isoform C (identifier: P41596-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     133-147: Missing.

Note: No experimental confirmation available.
Show »
Length:481
Mass (Da):52,894
Checksum:i86189AA2DBA4EDF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109G → V in ABP87886 (Ref. 4) Curated1
Sequence conflicti171 – 172ER → DG in CAA54451 (PubMed:7953290).Curated2
Sequence conflicti175R → P in CAA54451 (PubMed:7953290).Curated1
Sequence conflicti208Y → D in ABP87886 (Ref. 4) Curated1
Sequence conflicti354A → T in ABP87886 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti60I → L in strain: Canton-S. 1 Publication1
Natural varianti69T → I in strain: Canton-S. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0536451 – 15Missing in isoform C. CuratedAdd BLAST15
Alternative sequenceiVSP_037471133 – 147Missing in isoform B and isoform C. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77234 mRNA. Translation: CAA54451.1.
AE014297 Genomic DNA. Translation: AAF55030.3.
AE014297 Genomic DNA. Translation: ACZ94904.1.
BT030444 mRNA. Translation: ABP87886.1.
BT150144 mRNA. Translation: AGP25446.1.
U22106 mRNA. Translation: AAA85716.1.
PIRiS44275.
S68780.
RefSeqiNP_001163607.1. NM_001170136.2. [P41596-3]
NP_001247092.1. NM_001260163.1. [P41596-1]
NP_477007.2. NM_057659.4.
UniGeneiDm.3077.

Genome annotation databases

EnsemblMetazoaiFBtr0301351; FBpp0290565; FBgn0011582. [P41596-3]
FBtr0308191; FBpp0300511; FBgn0011582. [P41596-1]
GeneIDi41726.
KEGGidme:Dmel_CG9652.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDOPR1_DROME
AccessioniPrimary (citable) accession number: P41596
Secondary accession number(s): A4VCK3
, E1JIK5, Q24038, Q9VFM1, S4X4E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 23, 2004
Last modified: August 30, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. SIMILARITY comments
    Index of protein domains and families