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P41586 (PACR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pituitary adenylate cyclase-activating polypeptide type I receptor

Short name=PACAP type I receptor
Short name=PACAP-R-1
Short name=PACAP-R1
Gene names
Name:ADCYAP1R1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

Subunit structure

Interacts (via N-terminal extracellular domain) with ADCYAP1.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Sequence caution

The sequence BAA04466.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 468448Pituitary adenylate cyclase-activating polypeptide type I receptor
PRO_0000012841

Regions

Topological domain21 – 155135Extracellular Potential
Transmembrane156 – 17823Helical; Name=1; Potential
Topological domain179 – 1868Cytoplasmic Potential
Transmembrane187 – 20519Helical; Name=2; Potential
Topological domain206 – 22722Extracellular Potential
Transmembrane228 – 25326Helical; Name=3; Potential
Topological domain254 – 26815Cytoplasmic Potential
Transmembrane269 – 29123Helical; Name=4; Potential
Topological domain292 – 30918Extracellular Potential
Transmembrane310 – 33223Helical; Name=5; Potential
Topological domain333 – 35018Cytoplasmic Potential
Transmembrane351 – 37121Helical; Name=6; Potential
Topological domain372 – 38514Extracellular Potential
Transmembrane386 – 40520Helical; Name=7; Potential
Topological domain406 – 46863Cytoplasmic Potential
Region125 – 13915Important for ligand binding and specificity

Amino acid modifications

Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation3001N-linked (GlcNAc...) Potential
Glycosylation3751N-linked (GlcNAc...) Potential
Disulfide bond34 ↔ 63 Ref.7
Disulfide bond54 ↔ 118 Ref.7
Disulfide bond77 ↔ 134 Ref.7

Experimental info

Mutagenesis1141V → A: Reduced affinity for ADCYAP1. Ref.7
Mutagenesis1251E → R: Reduced affinity for ADCYAP1. Ref.7
Mutagenesis1281P → A: Reduced affinity for ADCYAP1. Ref.7
Mutagenesis1381E → R: Reduced affinity for ADCYAP1. Ref.7
Mutagenesis1391Y → A: Strongly reduced affinity for ADCYAP1. Ref.7

Secondary structure

........... 468
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P41586 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: BB515B84E9F28977

FASTA46853,314
        10         20         30         40         50         60 
MAGVVHVSLA ALLLLPMAPA MHSDCIFKKE QAMCLEKIQR ANELMGFNDS SPGCPGMWDN 

        70         80         90        100        110        120 
ITCWKPAHVG EMVLVSCPEL FRIFNPDQVW ETETIGESDF GDSNSLDLSD MGVVSRNCTE 

       130        140        150        160        170        180 
DGWSEPFPHY FDACGFDEYE SETGDQDYYY LSVKALYTVG YSTSLVTLTT AMVILCRFRK 

       190        200        210        220        230        240 
LHCTRNFIHM NLFVSFMLRA ISVFIKDWIL YAEQDSNHCF ISTVECKAVM VFFHYCVVSN 

       250        260        270        280        290        300 
YFWLFIEGLY LFTLLVETFF PERRYFYWYT IIGWGTPTVC VTVWATLRLY FDDTGCWDMN 

       310        320        330        340        350        360 
DSTALWWVIK GPVVGSIMVN FVLFIGIIVI LVQKLQSPDM GGNESSIYLR LARSTLLLIP 

       370        380        390        400        410        420 
LFGIHYTVFA FSPENVSKRE RLVFELGLGS FQGFVVAVLY CFLNGEVQAE IKRKWRSWKV 

       430        440        450        460 
NRYFAVDFKH RHPSLASSGV NGGTQLSILS KSSSQIRMSG LPADNLAT 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression of a cDNA encoding a human pituitary adenylate cyclase activating polypeptide receptor."
Ogi K., Miyamoto Y., Masuda Y., Habata Y., Hosoya M., Ohtaki T., Masuo Y., Onda H., Fujino M.
Biochem. Biophys. Res. Commun. 196:1511-1521(1993) [PubMed: 7902709] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
King M., Aronstam R.S., Sharma S.V.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hippocampus.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R): localization to chromosome band 7p14 and integration into the cytogenetic, physical and genetic map of chromosome 7."
Stoffel M., Espinosa R., Trabb J.B., le Beau M.M., Bell G.I.
Genomics 23:697-699(1994) [PubMed: 7851900] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-468.
Tissue: Placenta.
[7]"Solution structure and mutational analysis of pituitary adenylate cyclase-activating polypeptide binding to the extracellular domain of PAC1-RS."
Sun C., Song D., Davis-Taber R.A., Barrett L.W., Scott V.E., Richardson P.L., Pereda-Lopez A., Uchic M.E., Solomon L.R., Lake M.R., Walter K.A., Hajduk P.J., Olejniczak E.T.
Proc. Natl. Acad. Sci. U.S.A. 104:7875-7880(2007) [PubMed: 17470806] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-143 IN COMPLEX WITH ADCYAP1, MUTAGENESIS OF VAL-114; GLU-125; PRO-128; GLU-138 AND TYR-139, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17516 mRNA. Translation: BAA04466.1. Different initiation.
AB065700 Genomic DNA. Translation: BAC05923.1.
AY366498 mRNA. Translation: AAQ72806.1.
AK290046 mRNA. Translation: BAF82735.1.
CH471073 Genomic DNA. Translation: EAW93978.1.
U09216 Genomic DNA. Translation: AAA19323.1.
IPIIPI00299406.
PIRJN0902.
RefSeqNP_001109.2. NM_001118.4.
UniGeneHs.377783.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JODNMR-A22-143[»]
3N94X-ray1.80A26-140[»]
ProteinModelPortalP41586.
SMRP41586. Positions 26-140.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-1217291.
STRINGP41586.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP41586.

Polymorphism databases

DMDM1171986.

Proteomic databases

PRIDEP41586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000304166; ENSP00000306620; ENSG00000078549.
GeneID117.
KEGGhsa:117.
UCSCuc003tca.1. human.

Organism-specific databases

CTD117.
GeneCardsGC07P031058.
H-InvDBHIX0006574.
HGNCHGNC:242. ADCYAP1R1.
MIM102981. gene.
neXtProtNX_P41586.
PharmGKBPA24565.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG16465.
GeneTreeENSGT00580000081259.
HOVERGENHBG008318.
OrthoDBEOG4B5P5B.
PhylomeDBP41586.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP41586.
BgeeP41586.
CleanExHS_ADCYAP1R1.
GenevestigatorP41586.
GermOnlineENSG00000078549. Homo sapiens.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002285. GPCR_2_PACAP_1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
KOK04587.
PANTHERPTHR12011:SF30. PAC_1_rcpt. 1 hit.
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01156. PACAPRECEPTR.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio455.
SOURCESearch...

Entry information

Entry namePACR_HUMAN
AccessionPrimary (citable) accession number: P41586
Secondary accession number(s): A8K1Y1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 14, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families