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P41576 (6PGD_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
6-phosphogluconate dehydrogenase, decarboxylating
EC=1.1.1.44
Gene names
Name:gnd
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity.

Catalytic activity

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the 6-phosphogluconate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4684686-phosphogluconate dehydrogenase, decarboxylating
PRO_0000090043

Regions

Nucleotide binding10 – 156NADP By similarity
Nucleotide binding33 – 353NADP By similarity
Nucleotide binding74 – 763NADP By similarity
Region128 – 1303Substrate binding By similarity
Region186 – 1872Substrate binding By similarity

Sites

Active site1831Proton acceptor By similarity
Active site1901Proton donor By similarity
Binding site1021NADP By similarity
Binding site1021Substrate By similarity
Binding site1911Substrate By similarity
Binding site2601Substrate; via amide nitrogen By similarity
Binding site2871Substrate By similarity
Binding site4451Substrate; shared with dimeric partner By similarity
Binding site4511Substrate; shared with dimeric partner By similarity

Experimental info

Sequence conflict3161G → E in AAC43817. Ref.2
Sequence conflict4211V → F in AAC43817. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P41576 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: FF1EB5E7665FDC90

FASTA46851,328
        10         20         30         40         50         60 
MSKQQIGVVG MAVMGRNLAL NIESRGYTVS VFNRSREKTE EVIAENTGKK LVPYYTVQEF 

        70         80         90        100        110        120 
VESLETPRRI LLMVKAGAGT DSAIDSLKPY LDKGDIIIDG GNTFFQDTIR RNRELSAEGF 

       130        140        150        160        170        180 
NFIGTGVSGG EEGALKGPSI MPGGQKEAYE LVAPILKQIA AVAEDGEPCV TYIGADGAGH 

       190        200        210        220        230        240 
YVKMVHNGIE YGDMQLIAEA YALLKGGLAL SNEELAQTFT EWNEGELSSY LIDITKDIFT 

       250        260        270        280        290        300 
KKDEEGKYLV DVILDEAANK GTGKWTSQSS LDLGEPLSLI TESVFARYIS SLKDQRVAAS 

       310        320        330        340        350        360 
KVLSGPQAQP VGDKAGFIEK VRRALYLGKI VSYAQGFSQL RAASDEYNWD LNYGEIAKIF 

       370        380        390        400        410        420 
RAGCIIRAQF LQKITDAYAQ NAGIANLLLA PYFKQIADDY QQALRDVVAY AVQNGIPVPT 

       430        440        450        460 
VSAAIAYYDS YRSAVLPANL IQAQRDYFGA HTYKRTDKEG VFHTEWLE

« Hide

References

[1]"Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid."
Arakawa Y., Wacharotayankun R., Nagatsuka T., Ito H., Kato N., Ohta M.
J. Bacteriol. 177:1788-1796(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Chedid.
[2]"Intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene (gnd) in enteric bacteria."
Nelson K., Selander R.K.
Proc. Natl. Acad. Sci. U.S.A. 91:10227-10231(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-456.
Strain: CW 7380.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D21242 Genomic DNA. Translation: BAA04786.1.
U14471 Genomic DNA. Translation: AAC43817.1.
PIRD56146.

3D structure databases

ProteinModelPortalP41576.
SMRP41576. Positions 1-466.
ModBaseSearch...

Proteomic databases

PRIDEP41576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00115; UER00410.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFPIRSF000109. 6PGD. 1 hit.
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMsTIGR00873. gnd. 1 hit.
PROSITEPS00461. 6PGD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry name6PGD_KLEPN
AccessionPrimary (citable) accession number: P41576
Secondary accession number(s): Q48461
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: April 3, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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