Reviewed, UniProtKB/Swiss-Prot P41566 (IDH3G_PIG)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

IDH3G

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	106 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.
Enzyme regulation	Activated by increasing ADP/ATP ratios and by Ca(2+).
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	isocitrate metabolic process Ref.2 Inferred from mutant phenotype. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Ref.2 Inferred from direct assay. Source: UniProtKB
Molecular function	isocitrate dehydrogenase (NAD+) activity Ref.2 Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›106	›106	Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial		PRO_0000083592
Experimental info
	Non-adjacent residues	62 – 63	2
	Non-adjacent residues	75 – 76	2
	Non-adjacent residues	86 – 87	2
	Non-terminal residue	106	1

Sequences

	Sequence		Length	Mass (Da)
	P41566-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AB0A66AB845C49AB	FASTA	106	11,334
	10 20 30 40 50 60 FSQQTIPPSA KYGGILTVTM SPGDGDGPEL MLTVXXXXXS ACVPVDFEEV VVSSNADEED 70 80 90 100 IRTSLDLYAN VIHCKLGDGL FLQCCKNIAN PTATLLASCM MLDHLK

References

[1]	"Subunit location and sequences of the cysteinyl peptides of pig heart NAD-dependent isocitrate dehydrogenase." Huang Y.C., Colman R.F. Biochemistry 29:8266-8273(1990) [PubMed: 2252888] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Heart.
[2]	"Cysteinyl peptide labeled by 3-bromo-2-ketoglutarate in the active site of pig heart NAD+-dependent isocitrate dehydrogenase." Saha A., Huang Y.C., Colman R.F. Biochemistry 28:8425-8431(1989) [PubMed: 2605193] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-62. Tissue: Heart.
[3]	"Aspartyl peptide labeled by 2-(4-bromo-2,3-dioxobutylthio)adenosine 5'-diphosphate in the allosteric ADP site of pig heart NAD+-dependent isocitrate dehydrogenase." Huang Y.C., Colman R.F. J. Biol. Chem. 264:12208-12214(1989) [PubMed: 2745437] [Abstract] Cited for: PROTEIN SEQUENCE OF 76-86. Tissue: Heart.

Cross-references

Sequence databases
PIR	C35834.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P41566.
Family and domain databases
InterPro	IPR001804. IsoCit_IM_DHase. [Graphical view]
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
PROSITE	PS00470. IDH_IMDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3G_PIG

Accession

Primary (citable) accession number: P41566

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)