Reviewed, UniProtKB/Swiss-Prot P41565 (IDH3G_RAT)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

Idh3g

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Enzyme regulation	Activated by increasing ADP/ATP ratios and by Ca(2+).
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Manganese Metal-binding NAD Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	isocitrate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isocitrate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 393	354	Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial		PRO_0000014452
Regions
	Nucleotide binding	56 – 84	29	NAD Potential
	Nucleotide binding	309 – 316	8	ATP Potential
Sites
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Site	174	1	Critical for catalysis By similarity
	Site	221	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	363	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	7	1	I → V in CAA52225. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41565-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 890B1A4BE3543AE4	FASTA	393	42,851
	10 20 30 40 50 60 MALKVAIAAG SAAKAIFKPA LLCRPWEVLA AHEAPRRSIS SQQTIPPSAK YGGRHTVTMI 70 80 90 100 110 120 PGDGIGPELM LHVKSVFRHA CVPVDFEEVH VSSNADEEDI RNAIMAIRRN RVALKGNIET 130 140 150 160 170 180 NHDLPPSHKS RNNILRTSLD LYANVIHCKS LPGVVTRHKD IDILIVRENT EGEYSSLEHE 190 200 210 220 230 240 SVAGVVESLK IITKAKSLRI AEYAFKLAQE SGRKKVTAVH KANIMKLGDG LFLQCCREVA 250 260 270 280 290 300 ARYPQITFDS MIVDNTTMQL VSRPQQFDVM VMPNLYGNIV NNVCAGLVGG PGLVAGANYG 310 320 330 340 350 360 HVYAVFETAT RNTGKSIANK NIANPTATLL ASCMMLDHLK LHSYATSIRK AVLASMDNEN 370 380 390 MHTPDIGGQG TTSQAIQDII RHIRIINGRA VEA

References

[1]	"Genomic organization of two novel genes on human Xq28: compact head to head arrangement of IDH gamma and TRAP delta is conserved in rat and mouse." Brenner V., Nyakatura G., Rosenthal A., Platzer M. Genomics 44:8-14(1997) [PubMed: 9286695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24. Strain: HAN/WIST.
[2]	"Molecular cloning and deduced amino acid sequences of the gamma-subunits of rat and monkey NAD(+)-isocitrate dehydrogenases." Nichols B.J., Hall L., Perry A.C.F., Denton R.M. Biochem. J. 295:347-350(1993) [PubMed: 8240232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-393. Strain: Wistar. Tissue: Epididymis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63009 Genomic DNA. Translation: AAC53341.1. X74125 mRNA. Translation: CAA52225.1.
PIR	S39064.
UniGene	Rn.2837
3D structure databases
HSSP	HSSP built from PDB template 1OSJ based on UniProtKB P00351.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000037284. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	2863. Idh3g.
Phylogenomic databases
HOVERGEN	P41565.
Gene expression databases
ArrayExpress	P41565.
GermOnline	ENSRNOG00000037284. Rattus norvegicus.
Family and domain databases
InterPro	IPR004434. IsoCit_DHase_NAD_mit. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3G_RAT

Accession

Primary (citable) accession number: P41565
Secondary accession number(s): P70577

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 15, 1998
Last modified:	November 25, 2008
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)