Reviewed, UniProtKB/Swiss-Prot P41563 (IDH3A_BOVIN)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH
    Isocitrate dehydrogenase subunits 3/4

Gene names

Name:

IDH3A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: AgBase
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion
	Chain	28 – 366	339	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014435
Sites
	Metal binding	233	1	Magnesium or manganese By similarity
	Metal binding	257	1	Magnesium or manganese By similarity
	Metal binding	261	1	Magnesium or manganese By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity
	Site	153	1	Critical for catalysis By similarity
	Site	200	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41563-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B169D59B30E51C9F	FASTA	366	39,668
	10 20 30 40 50 60 MAGPAWISKV SRLLGAFHNQ KQVTRGFAGG VKTVTLIPGD GIGPEISAAV MKIFDAAKAP 70 80 90 100 110 120 IQWEERNVAA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK TPIAAGHPSM NLLLRKTFDL 130 140 150 160 170 180 YANVRPCVSI EGYKTPYHDV NIVTIRENTE GEYSGIEHVI VDGVVQSIKL ITEAASKRIA 190 200 210 220 230 240 EFAFEYARNN HRSNVTAVHK ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV 250 260 270 280 290 300 QDPSQFDVLV MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD 310 320 330 340 350 360 MANPTALLLS AVMMLRHMGL FDHAAKIETA CFATIKDGKS LTKDLGGNSK CSDFTEEICR RVKDLD

References

[1]	"Isocitrate dehydrogenase from bovine heart: primary structure of subunit 3/4." Zeng Y., Weiss C., Yao T.T., Huang J., Siconolfi-Baez L., Hsu P., Rushbrook J.I. Biochem. J. 310:507-516(1995) [PubMed: 7654189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Nicotinamide adenine dinucleotide dependent isocitrate dehydrogenase from beef heart: subunit heterogeneity and enzyme dissociation." Rushbrook J.I., Harvey R.A. Biochemistry 17:5339-5346(1978) [PubMed: 215197] [Abstract] Cited for: CHARACTERIZATION. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U07980 mRNA. Translation: AAC18425.1.
IPI	IPI00699016.
PIR	S58435.
RefSeq	NP_777069.1.
UniGene	Bt.3898
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000006227. Bos taurus. [Contig view]
GeneID	282446.
KEGG	bta:282446.
Phylogenomic databases
HOVERGEN	P41563.
Enzyme and pathway databases
BRENDA	1.1.1.41. 251.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3A_BOVIN

Accession

Primary (citable) accession number: P41563

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)