Isocitrate dehydrogenase [NADP] cytoplasmic

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Reviewed, UniProtKB/Swiss-Prot P41562 (IDHC_RAT)

Last modified June 16, 2009. Version 67. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] cytoplasmic
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Cytosolic NADP-isocitrate dehydrogenase
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:

Idh1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Ovary, mammary gland and liver.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	female gonad development Ref.1 Inferred from expression pattern. Source: RGD glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to organic cyclic substance Inferred from direct assay. Source: RGD response to steroid hormone stimulus Inferred from direct assay. Source: RGD tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from direct assay. Source: HGNC soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from direct assay. Source: RGD isocitrate dehydrogenase (NADP+) activity Ref.1 Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 414

414

Isocitrate dehydrogenase [NADP] cytoplasmic

PRO_0000083580

Regions

Nucleotide binding

75 – 77

NADP By similarity

Nucleotide binding

310 – 315

NADP By similarity

Region

94 – 100

Substrate binding By similarity

Sites

Metal binding

252

Magnesium or manganese By similarity

Metal binding

275

Magnesium or manganese By similarity

Binding site

Substrate By similarity

Binding site

NADP By similarity

Binding site

109

Substrate By similarity

Binding site

132

Substrate By similarity

Binding site

260

NADP By similarity

Binding site

328

NADP; via amide nitrogen and carbonyl oxygen By similarity

Site

139

Critical for catalysis By similarity

Site

212

Critical for catalysis By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P41562-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CF69EE8746DC1AF6
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FASTA

414

46,734

        10         20         30         40         50         60 
MSRKIHGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD ATNDQVTKDA 

        70         80         90        100        110        120 
AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 

       130        140        150        160        170        180 
VTGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPKD GSQKVTYLVH DFEEGGGVAM 

       190        200        210        220        230        240 
GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSKFE 

       250        260        270        280        290        300 
AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG 

       310        320        330        340        350        360 
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT ELSFFANALE 

       370        380        390        400        410 
EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKAKLA QAKL

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References

[1]	"Cytosolic NADP(+)-dependent isocitrate dehydrogenase. Isolation of rat cDNA and study of tissue-specific and developmental expression of mRNA." Jennings G.T., Sechi S., Stevenson P.M., Tuckey R.C., Parmelee D., McAlister-Henn L. J. Biol. Chem. 269:23128-23134(1994) [PubMed: 8083215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 389-400, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
[3]	"Structural characterization of cytosolic NADP(+)-dependent isocitrate dehydrogenase from rat ovary." Sechi S., Parmelee D., Roller P.R., Jennings G.T. Enzyme Protein 48:27-36(1995) [PubMed: 7787968] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Ovary.

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Cross-references

Sequence databases
	L35317 mRNA. Translation: AAA59356.1.
IPI	IPI00194045.
PIR	A54756.
RefSeq	NP_113698.1.
UniGene	Rn.3561
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
SMR	P41562. Positions 1-414.
ModBase	Search...
Proteomic databases
PRIDE	P41562.
Genome annotation databases
Ensembl	ENSRNOG00000015020. Rattus norvegicus. [Contig view]
GeneID	24479.
KEGG	rno:24479.
Organism-specific databases
RGD	2862. Idh1.
Phylogenomic databases
HOVERGEN	P41562.
Enzyme and pathway databases
BRENDA	1.1.1.42. 248.
Gene expression databases
ArrayExpress	P41562.
GermOnline	ENSRNOG00000015020. Rattus norvegicus.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603439.

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Entry information

Entry name

IDHC_RAT

Accession

Primary (citable) accession number: P41562
Secondary accession number(s): P80300

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents