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Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

Idh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateBy similarity1
Binding sitei82NADPBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Sitei139Critical for catalysisBy similarity1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1
Binding sitei260NADPBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPBy similarity3
Nucleotide bindingi310 – 315NADPBy similarity6

GO - Molecular functioni

  • isocitrate dehydrogenase (NADP+) activity Source: RGD
  • magnesium ion binding Source: UniProtKB
  • NAD binding Source: InterPro
  • NADP binding Source: RGD
  • protein homodimerization activity Source: Ensembl
  • signaling receptor binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.42 5301
ReactomeiR-RNO-389542 NADPH regeneration
R-RNO-6798695 Neutrophil degranulation
R-RNO-9033241 Peroxisomal protein import

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:Idh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2862 Idh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000835802 – 414Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei42PhosphotyrosineBy similarity1
Modified residuei81N6-acetyllysineBy similarity1
Modified residuei126N6-succinyllysineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei321N6-acetyllysineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei400N6-succinyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked.
Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP41562
PRIDEiP41562

PTM databases

iPTMnetiP41562
PhosphoSitePlusiP41562

Expressioni

Tissue specificityi

Ovary, mammary gland and liver.

Gene expression databases

BgeeiENSRNOG00000015020
GenevisibleiP41562 RN

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246640, 1 interactor
STRINGi10116.ENSRNOP00000020322

Structurei

3D structure databases

ProteinModelPortaliP41562
SMRiP41562
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526 Eukaryota
COG0538 LUCA
GeneTreeiENSGT00390000012547
HOGENOMiHOG000019858
HOVERGENiHBG006119
InParanoidiP41562
KOiK00031
OMAiAMGMYNQ
OrthoDBiEOG091G06IY
PhylomeDBiP41562

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKIHGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GSQKVTYLVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSKFE AQKIWYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFANALE EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Length:414
Mass (Da):46,734
Last modified:November 1, 1995 - v1
Checksum:iCF69EE8746DC1AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35317 mRNA Translation: AAA59356.1
PIRiA54756
RefSeqiNP_113698.1, NM_031510.1
XP_006245111.1, XM_006245049.3
XP_008765298.1, XM_008767076.2
UniGeneiRn.3561

Genome annotation databases

EnsembliENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020
GeneIDi24479
KEGGirno:24479
UCSCiRGD:2862 rat

Similar proteinsi

Entry informationi

Entry nameiIDHC_RAT
AccessioniPrimary (citable) accession number: P41562
Secondary accession number(s): P80300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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