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Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

Idh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateBy similarity1
Binding sitei82NADPBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Sitei139Critical for catalysisBy similarity1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1
Binding sitei260NADPBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPBy similarity3
Nucleotide bindingi310 – 315NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.42. 5301.
ReactomeiR-RNO-389542. NADPH regeneration.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:Idh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2862. Idh1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: Ensembl
  • cytosol Source: Ensembl
  • extracellular exosome Source: Ensembl
  • mitochondrion Source: Ensembl
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000835802 – 414Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei42PhosphotyrosineBy similarity1
Modified residuei81N6-acetyllysineBy similarity1
Modified residuei126N6-succinyllysineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1
Modified residuei233N6-acetyllysineBy similarity1
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei321N6-acetyllysineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei400N6-succinyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked.
Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP41562.
PRIDEiP41562.

PTM databases

iPTMnetiP41562.
PhosphoSitePlusiP41562.

Expressioni

Tissue specificityi

Ovary, mammary gland and liver.

Gene expression databases

BgeeiENSRNOG00000015020.
GenevisibleiP41562. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi246640. 1 interactor.
MINTiMINT-4565540.
STRINGi10116.ENSRNOP00000020322.

Structurei

3D structure databases

ProteinModelPortaliP41562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiP41562.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG091G06IY.
PhylomeDBiP41562.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKIHGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GSQKVTYLVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSKFE AQKIWYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFANALE EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Length:414
Mass (Da):46,734
Last modified:November 1, 1995 - v1
Checksum:iCF69EE8746DC1AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35317 mRNA. Translation: AAA59356.1.
PIRiA54756.
RefSeqiNP_113698.1. NM_031510.1.
XP_006245111.1. XM_006245049.3.
XP_008765298.1. XM_008767076.2.
UniGeneiRn.3561.

Genome annotation databases

EnsembliENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020.
GeneIDi24479.
KEGGirno:24479.
UCSCiRGD:2862. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35317 mRNA. Translation: AAA59356.1.
PIRiA54756.
RefSeqiNP_113698.1. NM_031510.1.
XP_006245111.1. XM_006245049.3.
XP_008765298.1. XM_008767076.2.
UniGeneiRn.3561.

3D structure databases

ProteinModelPortaliP41562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246640. 1 interactor.
MINTiMINT-4565540.
STRINGi10116.ENSRNOP00000020322.

PTM databases

iPTMnetiP41562.
PhosphoSitePlusiP41562.

Proteomic databases

PaxDbiP41562.
PRIDEiP41562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020.
GeneIDi24479.
KEGGirno:24479.
UCSCiRGD:2862. rat.

Organism-specific databases

CTDi3417.
RGDi2862. Idh1.

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiP41562.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG091G06IY.
PhylomeDBiP41562.

Enzyme and pathway databases

BRENDAi1.1.1.42. 5301.
ReactomeiR-RNO-389542. NADPH regeneration.
R-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP41562.

Gene expression databases

BgeeiENSRNOG00000015020.
GenevisibleiP41562. RN.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDHC_RAT
AccessioniPrimary (citable) accession number: P41562
Secondary accession number(s): P80300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.