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P41562 (IDHC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic
Short name=IDH
EC=1.1.1.42
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:Idh1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Tissue specificity

Ovary, mammary gland and liver.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Isocitrate dehydrogenase [NADP] cytoplasmic
PRO_0000083580

Regions

Nucleotide binding75 – 773NADP By similarity
Nucleotide binding310 – 3156NADP By similarity
Region94 – 1007Substrate binding By similarity

Sites

Metal binding2521Magnesium or manganese By similarity
Metal binding2751Magnesium or manganese By similarity
Binding site771Substrate By similarity
Binding site821NADP By similarity
Binding site1091Substrate By similarity
Binding site1321Substrate By similarity
Binding site2601NADP By similarity
Binding site3281NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1391Critical for catalysis By similarity
Site2121Critical for catalysis By similarity

Amino acid modifications

Modified residue811N6-acetyllysine By similarity
Modified residue2241N6-acetyllysine By similarity
Modified residue3211N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P41562 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CF69EE8746DC1AF6

FASTA41446,734
        10         20         30         40         50         60 
MSRKIHGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD ATNDQVTKDA 

        70         80         90        100        110        120 
AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 

       130        140        150        160        170        180 
VTGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPKD GSQKVTYLVH DFEEGGGVAM 

       190        200        210        220        230        240 
GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSKFE 

       250        260        270        280        290        300 
AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG 

       310        320        330        340        350        360 
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT ELSFFANALE 

       370        380        390        400        410 
EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKAKLA QAKL

« Hide

References

[1]"Cytosolic NADP(+)-dependent isocitrate dehydrogenase. Isolation of rat cDNA and study of tissue-specific and developmental expression of mRNA."
Jennings G.T., Sechi S., Stevenson P.M., Tuckey R.C., Parmelee D., McAlister-Henn L.
J. Biol. Chem. 269:23128-23134(1994) [PubMed: 8083215] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]Lubec G., Afjehi-Sadat L.
Submitted (NOV-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 389-400, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Spinal cord.
[3]"Structural characterization of cytosolic NADP(+)-dependent isocitrate dehydrogenase from rat ovary."
Sechi S., Parmelee D., Roller P.R., Jennings G.T.
Enzyme Protein 48:27-36(1994) [PubMed: 7787968] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L35317 mRNA. Translation: AAA59356.1.
IPIIPI00194045.
PIRA54756.
RefSeqNP_113698.1. NM_031510.1.
UniGeneRn.3561.

3D structure databases

ProteinModelPortalP41562.
SMRP41562. Positions 4-413.
ModBaseSearch...

Protein-protein interaction databases

STRINGP41562.

PTM databases

PhosphoSiteP41562.

Proteomic databases

PRIDEP41562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020.
GeneID24479.
KEGGrno:24479.
UCSCNM_031510. rat.

Organism-specific databases

CTD3417.
RGD2862. Idh1.

Phylogenomic databases

eggNOGroNOG04762.
HOVERGENHBG006119.
OrthoDBEOG47M1Z0.
PhylomeDBP41562.

Enzyme and pathway databases

BRENDA1.1.1.42. 5301.

Gene expression databases

ArrayExpressP41562.
GenevestigatorP41562.
GermOnlineENSRNOG00000015020. Rattus norvegicus.

Family and domain databases

InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP_euk-typ.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00031.
PANTHERPTHR11822. IDH_NADP_euk. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. Nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603439.

Entry information

Entry nameIDHC_RAT
AccessionPrimary (citable) accession number: P41562
Secondary accession number(s): P80300
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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