Reviewed, UniProtKB/Swiss-Prot P41561 (IDH2_COLMA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] 2
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase 2
    IDH-II
    NADP+-specific ICDH 2
    IDP-2

Gene names

Name:

icd2

Organism

Colwellia maris (Vibrio sp. (strain ABE-1))

Taxonomic identifier

77524 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the monomeric-type IDH family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 742	741	Isocitrate dehydrogenase [NADP] 2		PRO_0000083596
Regions
	Nucleotide binding	84 – 89	6	NADP By similarity
	Nucleotide binding	586 – 587	2	NADP By similarity
	Nucleotide binding	602 – 604	3	NADP By similarity
	Region	134 – 141	8	Substrate binding By similarity
Sites
	Metal binding	352	1	Magnesium or manganese By similarity
	Metal binding	550	1	Magnesium or manganese By similarity
	Binding site	137	1	NADP By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	549	1	Substrate By similarity
	Binding site	591	1	NADP By similarity
	Binding site	651	1	NADP By similarity
	Site	257	1	Critical for catalysis By similarity
	Site	422	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41561-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 63FC256EF2898583	FASTA	742	80,626
	10 20 30 40 50 60 MSTDNSKIIY TITDEAPALA TYSLLPIIQA YTASSGINVE TRDISLAGRI LANFPKYLTK 70 80 90 100 110 120 EQRIDDALAE LGELAQTPEA NIIKLPNISA SIPQLEAVIK ELQAKGYDLP HYPAEPQNEA 130 140 150 160 170 180 EESIKLTYAK ILGSAVNPVL REGNSDRRAP ASVKQYARNN PHSMGAWSKE SKSHVAHMAS 190 200 210 220 230 240 GDFYGSEKSV TIDGATSVNI EFVAKNGDVT LLKSKLPLLD KEIIDASVMS KSALVEFFET 250 260 270 280 290 300 EINKAKEEDV LLSLHLKATM MKVSDPVMFG HAVRVFYKDV FAKHAATFEQ LGVDADNGIG 310 320 330 340 350 360 DVYAKIARLP AAQKEEIEAD LQAVYATRPE MAMVDSDKGI TNLHVPSDVI IDASMPAALR 370 380 390 400 410 420 ASGMMWGPDG KQKDTKFMIP DRNYAGVFSA VVDFCRENGA FNPATMGTVP NVGLMAQKAE 430 440 450 460 470 480 EYGSHDKTFT MKAAGTVRVV NSQGERLIEQ EVAQGDIYRM CQVKDAPIQD WVKLAVTRAR 490 500 510 520 530 540 ATGTPTVFWL DENRGHDEQM IKKVNTYLAD HDTTGLDIQI LEPVKACEFT LARVAKGEDA 550 560 570 580 590 600 ISVTGNVLRD YLTDLFPILE LGTSAKMLSI VPLMNGGGLF ETGAGGSAPK HVQQFEKENH 610 620 630 640 650 660 LRWDSLGEFL ALAASLEHVA VTTGNARAQI LADTLDAATG KFLDTNKSPS RKVGELDNRV 670 680 690 700 710 720 VTSILQCIGR NVAAQTTDTE LQASFSSVAQ ALTKQEEKIV AELNAAQGPA IDLNGYYFAD 730 740 TKLAEKAMRP SETFNTILSA LL

References

[1]	"Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1." Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N. J. Bacteriol. 175:6873-6880(1993) [PubMed: 8226630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and characterization of monomeric isocitrate dehydrogenase with NADP(+)-specificity from Vibrio parahaemolyticus Y-4." Fukunaga N., Imagawa S., Sahara T., Ishii A., Suzuki M. J. Biochem. 112:849-855(1992) [PubMed: 1295895] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-39.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D14047 Genomic DNA. Translation: BAA03134.1.
PIR	A49341.
3D structure databases
HSSP	HSSP built from PDB template 1ITW based on UniProtKB P16100.
ModBase	Search...
Family and domain databases
InterPro	IPR004436. IsoCit_DHase_NAD_mono. [Graphical view]
Pfam	PF03971. IDH. 1 hit. [Graphical view]
PIRSF	PIRSF009407. IDH_monmr. 1 hit.
TIGRFAMs	TIGR00178. monomer_idh. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IDH2_COLMA

Accession

Primary (citable) accession number: P41561

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)