P41561 (IDH2_COLMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] 2
EC=1.1.1.42

Alternative name(s):
IDH-II
IDP-2
NADP(+)-specific ICDH 2
Oxalosuccinate decarboxylase 2

Gene names

Name:

icd2

Organism

Colwellia maris (Vibrio sp. (strain ABE-1))

Taxonomic identifier

77524 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the monomeric-type IDH family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 743	742	Isocitrate dehydrogenase [NADP] 2		PRO_0000083596
Regions
	Nucleotide binding	84 – 89	6	NADP By similarity
	Nucleotide binding	586 – 587	2	NADP By similarity
	Nucleotide binding	602 – 604	3	NADP By similarity
	Region	134 – 141	8	Substrate binding By similarity
Sites
	Metal binding	352	1	Magnesium or manganese By similarity
	Metal binding	550	1	Magnesium or manganese By similarity
	Metal binding	554	1	Magnesium or manganese By similarity
	Binding site	137	1	NADP By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	549	1	Substrate By similarity
	Binding site	591	1	NADP By similarity
	Binding site	651	1	NADP By similarity
	Site	257	1	Critical for catalysis By similarity
	Site	422	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41561 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: DE1E71FF4B5A6E06	FASTA	743	80,882
	10 20 30 40 50 60 MSTDNSKIIY TITDEAPALA TYSLLPIIQA YTASSGINVE TRDISLAGRI LANFPKYLTK 70 80 90 100 110 120 EQRIDDALAE LGELAQTPEA NIIKLPNISA SIPQLEAVIK ELQAKGYDLP HYPAEPQNEA 130 140 150 160 170 180 EESIKLTYAK ILGSAVNPVL REGNSDRRAP ASVKQYARNN PHSMGAWSKE SKSHVAHMAS 190 200 210 220 230 240 GDFYGSEKSV TIDGATSVNI EFVAKNGDVT LLKSKLPLLD KEIIDASVMS KSALVEFFET 250 260 270 280 290 300 EINKAKEEDV LLSLHLKATM MKVSDPVMFG HAVRVFYKDV FAKHAATFEQ LGVDADNGIG 310 320 330 340 350 360 DVYAKIARLP AAQKEEIEAD LQAVYATRPE MAMVDSDKGI TNLHVPSDVI IDASMPAALR 370 380 390 400 410 420 ASGMMWGPDG KQKDTKFMIP DRNYAGVFSA VVDFCRENGA FNPATMGTVP NVGLMAQKAE 430 440 450 460 470 480 EYGSHDKTFT MKAAGTVRVV NSQGERLIEQ EVAQGDIYRM CQVKDAPIQD WVKLAVTRAR 490 500 510 520 530 540 ATGTPTVFWL DENRGHDEQM IKKVNTYLAD HDTTGLDIQI LEPVKACEFT LARVAKGEDA 550 560 570 580 590 600 ISVTGNVLRD YLTDLFPILE LGTSAKMLSI VPLMNGGGLF ETGAGGSAPK HVQQFEKENH 610 620 630 640 650 660 LRWDSLGEFL ALAASLEHVA VTTGNARAQI LADTLDAATG KFLDTNKSPS RKVGELDNRG 670 680 690 700 710 720 SHFYLAMYWA QALAAQTTDT ELQASFSSVA QALTKQEEKI VAELNAAQGP AIDLNGYYFA 730 740 DTKLAEKAMR PSETFNTILS ALL

References

[1]	"Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1." Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N. J. Bacteriol. 175:6873-6880(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 660-673.
[3]	"Purification and characterization of monomeric isocitrate dehydrogenase with NADP(+)-specificity from Vibrio parahaemolyticus Y-4." Fukunaga N., Imagawa S., Sahara T., Ishii A., Suzuki M. J. Biochem. 112:849-855(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-39.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14047 Genomic DNA. Translation: BAA03134.2.
PIR	A49341.
3D structure databases
ProteinModelPortal	P41561.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.718.10. 3 hits.
InterPro	IPR004436. Isocitrate_DH_NADP_mono. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
Pfam	PF03971. IDH. 1 hit. [Graphical view]
PIRSF	PIRSF009407. IDH_monmr. 1 hit.
TIGRFAMs	TIGR00178. monomer_idh. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IDH2_COLMA

Accession

Primary (citable) accession number: P41561

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 14, 2009
Last modified:	April 3, 2013
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program