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P41560

- IDH1_COLMA

UniProt

P41560 - IDH1_COLMA

Protein

Isocitrate dehydrogenase [NADP] 1

Gene

icdI

Organism
Colwellia maris (Vibrio sp. (strain ABE-1))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041NADPBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei115 – 1151SubstrateBy similarity
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei129 – 1291SubstrateBy similarity
    Binding sitei153 – 1531SubstrateBy similarity
    Sitei160 – 1601Critical for catalysisBy similarity
    Sitei230 – 2301Critical for catalysisBy similarity
    Metal bindingi307 – 3071Magnesium or manganeseBy similarity
    Binding sitei352 – 3521NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei390 – 3901NADPBy similarity
    Binding sitei394 – 3941NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi339 – 3457NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] 1 (EC:1.1.1.42)
    Alternative name(s):
    IDH-I
    IDP-1
    NADP(+)-specific ICDH 1
    Oxalosuccinate decarboxylase 1
    Gene namesi
    Name:icdI
    OrganismiColwellia maris (Vibrio sp. (strain ABE-1))
    Taxonomic identifieri77524 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeColwellia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 415414Isocitrate dehydrogenase [NADP] 1PRO_0000083550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei113 – 1131PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP41560.

    Expressioni

    Inductioni

    By acetate.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP41560.
    SMRiP41560. Positions 3-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004439. Isocitrate_DH_NADP_dimer_prok.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNKIIIPTT GDKITFIDGK LSVPNNPIIP YIEGDGIGVD VTPPMLKVVN    50
    AAVAKAYGGD RKIEWLEVYA GEKATKMYDS ETWLPEETLN ILQEYKVSIK 100
    GPLTTPVGGG MSSLNVAIRQ MLDLYVCQRP VQWFTGVPSP VKRPSEVDMV 150
    IFRENTEDIY AGIEYKAGSD KAKSVIKFLI EEMGASNIRF TENCGIGIKP 200
    VSKEGSQRLV RQAIQYAIDN NKDSVTLVHK GNIMKFTEGA FKDWGYELAI 250
    EEFGASLLHG GPWCSLKNPN TGKEIIIKDV IADAMLQQVL LRPAEYSVIA 300
    TLNLNGDYLS DALAAQVGGI GIAPGANLGD EVAVFEATHG TAPKYAGKNK 350
    VNPGSVILSA EMMLRHMGWL EADLLLKGMS GAIQAKTVTY DFERLMDDAT 400
    LVSCSAFGDC IIDHM 415
    Length:415
    Mass (Da):45,145
    Last modified:January 23, 2007 - v2
    Checksum:i353EFF202824696E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261N → G AA sequence (PubMed:1295895)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14047 Genomic DNA. Translation: BAA03135.1.
    PIRiB49341.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14047 Genomic DNA. Translation: BAA03135.1 .
    PIRi B49341.

    3D structure databases

    ProteinModelPortali P41560.
    SMRi P41560. Positions 3-415.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P41560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004439. Isocitrate_DH_NADP_dimer_prok.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00183. prok_nadp_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1."
      Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N.
      J. Bacteriol. 175:6873-6880(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Purification and characterization of monomeric isocitrate dehydrogenase with NADP(+)-specificity from Vibrio parahaemolyticus Y-4."
      Fukunaga N., Imagawa S., Sahara T., Ishii A., Suzuki M.
      J. Biochem. 112:849-855(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-41.

    Entry informationi

    Entry nameiIDH1_COLMA
    AccessioniPrimary (citable) accession number: P41560
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3