Reviewed, UniProtKB/Swiss-Prot P41560 (IDH1_COLMA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] 1
    EC=1.1.1.42
Alternative name(s):
    IDH-I
    Oxalosuccinate decarboxylase 1
    NADP(+)-specific ICDH 1
    IDP-1

Gene names

Name:

icdI

Organism

Colwellia maris (Vibrio sp. (strain ABE-1))

Taxonomic identifier

77524 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer.
Induction	By acetate.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 415	414	Isocitrate dehydrogenase [NADP] 1		PRO_0000083550
Regions
	Nucleotide binding	339 – 345	7	NADP By similarity
Sites
	Metal binding	307	1	Magnesium or manganese By similarity
	Binding site	104	1	NADP By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	352	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	390	1	NADP By similarity
	Binding site	394	1	NADP By similarity
	Site	160	1	Critical for catalysis By similarity
	Site	230	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	113	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	26	1	N → G AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41560-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 353EFF202824696E	FASTA	415	45,145
	10 20 30 40 50 60 MTNKIIIPTT GDKITFIDGK LSVPNNPIIP YIEGDGIGVD VTPPMLKVVN AAVAKAYGGD 70 80 90 100 110 120 RKIEWLEVYA GEKATKMYDS ETWLPEETLN ILQEYKVSIK GPLTTPVGGG MSSLNVAIRQ 130 140 150 160 170 180 MLDLYVCQRP VQWFTGVPSP VKRPSEVDMV IFRENTEDIY AGIEYKAGSD KAKSVIKFLI 190 200 210 220 230 240 EEMGASNIRF TENCGIGIKP VSKEGSQRLV RQAIQYAIDN NKDSVTLVHK GNIMKFTEGA 250 260 270 280 290 300 FKDWGYELAI EEFGASLLHG GPWCSLKNPN TGKEIIIKDV IADAMLQQVL LRPAEYSVIA 310 320 330 340 350 360 TLNLNGDYLS DALAAQVGGI GIAPGANLGD EVAVFEATHG TAPKYAGKNK VNPGSVILSA 370 380 390 400 410 EMMLRHMGWL EADLLLKGMS GAIQAKTVTY DFERLMDDAT LVSCSAFGDC IIDHM

References

[1]	"Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1." Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N. J. Bacteriol. 175:6873-6880(1993) [PubMed: 8226630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and characterization of monomeric isocitrate dehydrogenase with NADP(+)-specificity from Vibrio parahaemolyticus Y-4." Fukunaga N., Imagawa S., Sahara T., Ishii A., Suzuki M. J. Biochem. 112:849-855(1992) [PubMed: 1295895] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-41.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D14047 Genomic DNA. Translation: BAA03135.1.
PIR	B49341.
3D structure databases
HSSP	HSSP built from PDB template 1PB1 based on UniProtKB P08200.
SMR	P41560. Positions 1-415.
ModBase	Search...
Family and domain databases
InterPro	IPR004439. IsoCit_DHase_NADP_prok. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH1_COLMA

Accession

Primary (citable) accession number: P41560

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)