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Protein

Isocitrate lyase

Gene

icl

Organism
Rhodococcus fascians
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumBy similarity
Active sitei191 – 1911Proton acceptorBy similarity
Binding sitei228 – 2281SubstrateBy similarity
Binding sitei347 – 3471SubstrateBy similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)
Short name:
ICL1 Publication
Alternative name(s):
IsocitraseBy similarity
IsocitrataseBy similarity
Gene namesi
Name:icl1 Publication
OrganismiRhodococcus fascians
Taxonomic identifieri1828 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Isocitrate lyasePRO_0000068781Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP41554.
SMRiP41554. Positions 2-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Substrate bindingBy similarity
Regioni192 – 1932Substrate bindingBy similarity
Regioni313 – 3175Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTGTPKTT AEIQQDWDTN PRWKGVTRNF TAQQVSDLQG TVVEEATLAR
60 70 80 90 100
RGSEILWDLV NNEDYINSLG ALTGNQAVQQ IRAGLQAIYL SGWQVAGDAN
110 120 130 140 150
LSGHTYPDQS LYPANSVPSV VRRINNALLR ADEIAKIEGD TSVKNWVAPI
160 170 180 190 200
VADAEAGFGG ALNAYELQKA MIVAGAAGVH WEDQLASEKK CGHLGGKVLI
210 220 230 240 250
PTQQHIRTLT SARLASDVAD VPSVIIARTD AEAATLITSD VDERDREFLD
260 270 280 290 300
GTRTAEGFFG VKNGIEPCIA RAKAYAPYAD LIWMETGVPD LEVAKKFSES
310 320 330 340 350
VRSEFPDQLL AYNWSPSFNW KAHLDDATIA KFQKELGAMG FKFQFITLAG
360 370 380 390 400
FHSLNYGMFD LAHGYAREGM TAFVDLQERE FKAAEERGFT AIKHQREVGA
410 420
GYFDRIATTV DPNTSTAALK GSTEEGQFH
Length:429
Mass (Da):46,900
Last modified:October 31, 1995 - v1
Checksum:iEDBD9D5A2FF6BA0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29367 Genomic DNA. Translation: CAA82555.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29367 Genomic DNA. Translation: CAA82555.1.

3D structure databases

ProteinModelPortaliP41554.
SMRiP41554. Positions 2-428.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of the gene encoding isocitrate lyase from Rhodococcus fascians."
    Vereecke D., Villarroel R., van Montagu M., Desomer J.
    Gene 145:109-114(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D188.

Entry informationi

Entry nameiACEA_RHOFA
AccessioniPrimary (citable) accession number: P41554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: March 31, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.