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P41546 (HAC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator HAC1
Gene names
Name:HAC1
Synonyms:ERN4, IRE15, IRE2
Ordered Locus Names:YFL031W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator involved in the unfolded protein response (UPR) pathway. Recognizes and binds to the UPR element (UPRE) in the promoter of UPR-regulated genes such as KAR2, PDI1, EUG1 and FKB2. Increases the synthesis of endoplasmic reticulum-resident proteins required for protein folding as well as components of the secretory pathway.

Subunit structure

Homodimer.

Subcellular location

Nucleus Ref.12.

Induction

By the unfolded protein response pathway. Accumulation of unfolded proteins in the ER leads to activation of IRE1, which initiates splicing of the untranslated HAC1 precursor mRNA to produce the mature form. Ref.10 Ref.11

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the bZIP family.

Contains 1 bZIP (basic-leucine zipper) domain.

Sequence caution

The sequence BAA05513.1 differs from that shown. Reason: Frameshift at positions 143, 151, 165 and 182.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Splicing occurs by a non-spliceosomal, regulated splicing mechanism. When UPR is induced, the mRNA is cleaved by bifunctional transmembrane kinase/endoribonuclease IRE1 and the exons are joined by tRNA ligase TRL1.
Isoform I (identifier: P41546-2)

Also known as: Induced;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Induced and active isoform.
Isoform U (identifier: P41546-1)

Also known as: Uninduced;

The sequence of this isoform differs from the canonical sequence as follows:
     221-238: EAQSGLNSFELNDFFITS → AVITMTRKLQ
Note: Not translated. The unspliced HAC1 mRNA is stable, located in the cytoplasm, and is associated with polyribosomes, yet does not produce protein. Translational attenuation is due to basepairing of the intron with the 5'-UTR of the mRNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Transcriptional activator HAC1
PRO_0000076518

Regions

Domain39 – 10264bZIP
Region41 – 6121Basic motif By similarity
Region67 – 748Leucine-zipper By similarity

Natural variations

Alternative sequence221 – 23818EAQSG…FFITS → AVITMTRKLQ in isoform U.
VSP_020905

Experimental info

Sequence conflict1811D → Y in BAA05513. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform I (Induced) [UniParc].

Last modified October 3, 2006. Version 2.
Checksum: 81C01ACB3E2531B1

FASTA23826,905
        10         20         30         40         50         60 
MEMTDFELTS NSQSNLAIPT NFKSTLPPRK RAKTKEEKEQ RRIERILRNR RAAHQSREKK 

        70         80         90        100        110        120 
RLHLQYLERK CSLLENLLNS VNLEKLADHE DALTCSHDAF VASLDEYRDF QSTRGASLDT 

       130        140        150        160        170        180 
RASSHSSSDT FTPSPLNCTM EPATLSPKSM RDSASDQETS WELQMFKTEN VPESTTLPAV 

       190        200        210        220        230 
DNNNLFDAVA SPLADPLCDD IAGNSLPFDN SIDLDNWRNP EAQSGLNSFE LNDFFITS 

« Hide

Isoform U (Uninduced) [UniParc].

Checksum: D6CFD739C133AB4B
Show »

FASTA23026,046

References

« Hide 'large scale' references
[1]"Hac1: a novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdc10 mutant of Schizosaccharomyces pombe."
Nojima H., Leem S.-H., Araki H., Sakai A., Nakashima N., Kanaoka Y., Ono Y.
Nucleic Acids Res. 22:5279-5288(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Murakami Y.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 192-238.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Signalling from endoplasmic reticulum to nucleus: transcription factor with a basic-leucine zipper motif is required for the unfolded protein-response pathway."
Mori K., Kawahara T., Yoshida H., Yanagi H., Yura T.
Genes Cells 1:803-817(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204510 / AB320.
[6]"Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression."
Nikawa J., Akiyoshi M., Hirata S., Fukuda T.
Nucleic Acids Res. 24:4222-4226(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response."
Cox J.S., Walter P.
Cell 87:391-404(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, ALTERNATIVE SPLICING.
[8]"tRNA ligase is required for regulated mRNA splicing in the unfolded protein response."
Sidrauski C., Cox J.S., Walter P.
Cell 87:405-413(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, MRNA LIGATION BY TRL1.
[9]"The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response."
Sidrauski C., Walter P.
Cell 90:1031-1039(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, MRNA CLEAVAGE BY IRE1.
[10]"Endoplasmic reticulum stress-induced mRNA splicing permits synthesis of transcription factor Hac1p/Ern4p that activates the unfolded protein response."
Kawahara T., Yanagi H., Yura T., Mori K.
Mol. Biol. Cell 8:1845-1862(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, INDUCTION.
[11]"Block of HAC1 mRNA translation by long-range base pairing is released by cytoplasmic splicing upon induction of the unfolded protein response."
Rueegsegger U., Leber J.H., Walter P.
Cell 107:103-114(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, INDUCTION.
[12]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26506 Genomic DNA. Translation: BAA05513.1. Frameshift.
D50617 Genomic DNA. Translation: BAA24425.1.
D86413 Genomic DNA. Translation: BAA19565.1.
BK006940 Genomic DNA. Translation: DAA12409.1.
PIRS78571.
RefSeqNP_116622.1. NM_001179935.1.

3D structure databases

ProteinModelPortalP41546.
SMRP41546. Positions 41-84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31115. 297 interactions.
DIPDIP-2203N.
IntActP41546. 3 interactions.
MINTMINT-4484879.
STRING4932.YFL031W.

Proteomic databases

PaxDbP41546.
PeptideAtlasP41546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL031W; YFL031W; YFL031W. [P41546-2]
GeneID850513.
KEGGsce:YFL031W.

Organism-specific databases

CYGDYFL031w.
SGDS000001863. HAC1.

Phylogenomic databases

eggNOGNOG247265.
HOGENOMHOG000112845.
KOK16230.
OrthoDBEOG7PCJV2.

Enzyme and pathway databases

BioCycYEAST:G3O-30430-MONOMER.

Gene expression databases

GenevestigatorP41546.

Family and domain databases

InterProIPR004827. bZIP.
[Graphical view]
PfamPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966230.

Entry information

Entry nameHAC1_YEAST
AccessionPrimary (citable) accession number: P41546
Secondary accession number(s): D6VTJ9, P87040
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families