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P41546

- HAC1_YEAST

UniProt

P41546 - HAC1_YEAST

Protein

Transcriptional activator HAC1

Gene

HAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Transcriptional activator involved in the unfolded protein response (UPR) pathway. Recognizes and binds to the UPR element (UPRE) in the promoter of UPR-regulated genes such as KAR2, PDI1, EUG1 and FKB2. Increases the synthesis of endoplasmic reticulum-resident proteins required for protein folding as well as components of the secretory pathway.

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: SGD

    GO - Biological processi

    1. endoplasmic reticulum unfolded protein response Source: SGD
    2. negative regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Source: SGD
    5. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Unfolded protein response

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30430-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator HAC1
    Gene namesi
    Name:HAC1
    Synonyms:ERN4, IRE15, IRE2
    Ordered Locus Names:YFL031W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL031w.
    SGDiS000001863. HAC1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Transcriptional activator HAC1PRO_0000076518Add
    BLAST

    Proteomic databases

    PaxDbiP41546.
    PeptideAtlasiP41546.

    Expressioni

    Inductioni

    By the unfolded protein response pathway. Accumulation of unfolded proteins in the ER leads to activation of IRE1, which initiates splicing of the untranslated HAC1 precursor mRNA to produce the mature form.2 Publications

    Gene expression databases

    GenevestigatoriP41546.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi31115. 297 interactions.
    DIPiDIP-2203N.
    IntActiP41546. 3 interactions.
    MINTiMINT-4484879.
    STRINGi4932.YFL031W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41546.
    SMRiP41546. Positions 47-84.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 10264bZIPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 6121Basic motifBy similarityAdd
    BLAST
    Regioni67 – 748Leucine-zipperBy similarity

    Sequence similaritiesi

    Belongs to the bZIP family.Curated

    Phylogenomic databases

    eggNOGiNOG247265.
    HOGENOMiHOG000112845.
    KOiK16230.
    OrthoDBiEOG7PCJV2.

    Family and domain databases

    InterProiIPR004827. bZIP.
    [Graphical view]
    PfamiPF07716. bZIP_2. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Splicing occurs by a non-spliceosomal, regulated splicing mechanism. When UPR is induced, the mRNA is cleaved by bifunctional transmembrane kinase/endoribonuclease IRE1 and the exons are joined by tRNA ligase TRL1.

    Isoform I (identifier: P41546-2) [UniParc]FASTAAdd to Basket

    Also known as: Induced

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEMTDFELTS NSQSNLAIPT NFKSTLPPRK RAKTKEEKEQ RRIERILRNR    50
    RAAHQSREKK RLHLQYLERK CSLLENLLNS VNLEKLADHE DALTCSHDAF 100
    VASLDEYRDF QSTRGASLDT RASSHSSSDT FTPSPLNCTM EPATLSPKSM 150
    RDSASDQETS WELQMFKTEN VPESTTLPAV DNNNLFDAVA SPLADPLCDD 200
    IAGNSLPFDN SIDLDNWRNP EAQSGLNSFE LNDFFITS 238

    Note: Induced and active isoform.

    Length:238
    Mass (Da):26,905
    Last modified:October 3, 2006 - v2
    Checksum:i81C01ACB3E2531B1
    GO
    Isoform U (identifier: P41546-1) [UniParc]FASTAAdd to Basket

    Also known as: Uninduced

    The sequence of this isoform differs from the canonical sequence as follows:
         221-238: EAQSGLNSFELNDFFITS → AVITMTRKLQ

    Note: Not translated. The unspliced HAC1 mRNA is stable, located in the cytoplasm, and is associated with polyribosomes, yet does not produce protein. Translational attenuation is due to basepairing of the intron with the 5'-UTR of the mRNA.

    Show »
    Length:230
    Mass (Da):26,046
    Checksum:iD6CFD739C133AB4B
    GO

    Sequence cautioni

    The sequence BAA05513.1 differs from that shown. Reason: Frameshift at positions 143, 151, 165 and 182.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811D → Y in BAA05513. (PubMed:7816617)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei221 – 23818EAQSG…FFITS → AVITMTRKLQ in isoform U. CuratedVSP_020905Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26506 Genomic DNA. Translation: BAA05513.1. Frameshift.
    D50617 Genomic DNA. Translation: BAA24425.1.
    D86413 Genomic DNA. Translation: BAA19565.1.
    BK006940 Genomic DNA. Translation: DAA12409.1.
    PIRiS78571.
    RefSeqiNP_116622.1. NM_001179935.1. [P41546-2]

    Genome annotation databases

    EnsemblFungiiYFL031W; YFL031W; YFL031W. [P41546-2]
    GeneIDi850513.
    KEGGisce:YFL031W.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26506 Genomic DNA. Translation: BAA05513.1 . Frameshift.
    D50617 Genomic DNA. Translation: BAA24425.1 .
    D86413 Genomic DNA. Translation: BAA19565.1 .
    BK006940 Genomic DNA. Translation: DAA12409.1 .
    PIRi S78571.
    RefSeqi NP_116622.1. NM_001179935.1. [P41546-2 ]

    3D structure databases

    ProteinModelPortali P41546.
    SMRi P41546. Positions 47-84.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31115. 297 interactions.
    DIPi DIP-2203N.
    IntActi P41546. 3 interactions.
    MINTi MINT-4484879.
    STRINGi 4932.YFL031W.

    Proteomic databases

    PaxDbi P41546.
    PeptideAtlasi P41546.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL031W ; YFL031W ; YFL031W . [P41546-2 ]
    GeneIDi 850513.
    KEGGi sce:YFL031W.

    Organism-specific databases

    CYGDi YFL031w.
    SGDi S000001863. HAC1.

    Phylogenomic databases

    eggNOGi NOG247265.
    HOGENOMi HOG000112845.
    KOi K16230.
    OrthoDBi EOG7PCJV2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30430-MONOMER.

    Miscellaneous databases

    NextBioi 966230.

    Gene expression databases

    Genevestigatori P41546.

    Family and domain databases

    InterProi IPR004827. bZIP.
    [Graphical view ]
    Pfami PF07716. bZIP_2. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Hac1: a novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdc10 mutant of Schizosaccharomyces pombe."
      Nojima H., Leem S.-H., Araki H., Sakai A., Nakashima N., Kanaoka Y., Ono Y.
      Nucleic Acids Res. 22:5279-5288(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Murakami Y.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 192-238.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Signalling from endoplasmic reticulum to nucleus: transcription factor with a basic-leucine zipper motif is required for the unfolded protein-response pathway."
      Mori K., Kawahara T., Yoshida H., Yanagi H., Yura T.
      Genes Cells 1:803-817(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204510 / AB320.
    6. "Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression."
      Nikawa J., Akiyoshi M., Hirata S., Fukuda T.
      Nucleic Acids Res. 24:4222-4226(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response."
      Cox J.S., Walter P.
      Cell 87:391-404(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, ALTERNATIVE SPLICING.
    8. "tRNA ligase is required for regulated mRNA splicing in the unfolded protein response."
      Sidrauski C., Cox J.S., Walter P.
      Cell 87:405-413(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, MRNA LIGATION BY TRL1.
    9. "The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response."
      Sidrauski C., Walter P.
      Cell 90:1031-1039(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, MRNA CLEAVAGE BY IRE1.
    10. "Endoplasmic reticulum stress-induced mRNA splicing permits synthesis of transcription factor Hac1p/Ern4p that activates the unfolded protein response."
      Kawahara T., Yanagi H., Yura T., Mori K.
      Mol. Biol. Cell 8:1845-1862(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, INDUCTION.
    11. "Block of HAC1 mRNA translation by long-range base pairing is released by cytoplasmic splicing upon induction of the unfolded protein response."
      Rueegsegger U., Leber J.H., Walter P.
      Cell 107:103-114(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, INDUCTION.
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHAC1_YEAST
    AccessioniPrimary (citable) accession number: P41546
    Secondary accession number(s): D6VTJ9, P87040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3