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Protein

Transcriptional activator HAC1

Gene

HAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator involved in the unfolded protein response (UPR) pathway. Recognizes and binds to the UPR element (UPRE) in the promoter of UPR-regulated genes such as KAR2, PDI1, EUG1 and FKB2. Increases the synthesis of endoplasmic reticulum-resident proteins required for protein folding as well as components of the secretory pathway.

GO - Molecular functioni

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator HAC1
Gene namesi
Name:HAC1
Synonyms:ERN4, IRE15, IRE2
Ordered Locus Names:YFL031W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

CYGDiYFL031w.
EuPathDBiFungiDB:YFL031W.
SGDiS000001863. HAC1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Transcriptional activator HAC1PRO_0000076518Add
BLAST

Proteomic databases

PaxDbiP41546.
PeptideAtlasiP41546.

Expressioni

Inductioni

By the unfolded protein response pathway. Accumulation of unfolded proteins in the ER leads to activation of IRE1, which initiates splicing of the untranslated HAC1 precursor mRNA to produce the mature form.2 Publications

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31115. 300 interactions.
DIPiDIP-2203N.
IntActiP41546. 3 interactions.
MINTiMINT-4484879.

Structurei

3D structure databases

ProteinModelPortaliP41546.
SMRiP41546. Positions 47-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 10264bZIPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 6121Basic motifBy similarityAdd
BLAST
Regioni67 – 748Leucine-zipperBy similarity

Sequence similaritiesi

Belongs to the bZIP family.Curated

Phylogenomic databases

eggNOGiNOG247265.
HOGENOMiHOG000112845.
InParanoidiP41546.
KOiK16230.
OrthoDBiEOG7PCJV2.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Splicing occurs by a non-spliceosomal, regulated splicing mechanism. When UPR is induced, the mRNA is cleaved by bifunctional transmembrane kinase/endoribonuclease IRE1 and the exons are joined by tRNA ligase TRL1.

Isoform I (identifier: P41546-2) [UniParc]FASTAAdd to basket

Also known as: Induced

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMTDFELTS NSQSNLAIPT NFKSTLPPRK RAKTKEEKEQ RRIERILRNR
60 70 80 90 100
RAAHQSREKK RLHLQYLERK CSLLENLLNS VNLEKLADHE DALTCSHDAF
110 120 130 140 150
VASLDEYRDF QSTRGASLDT RASSHSSSDT FTPSPLNCTM EPATLSPKSM
160 170 180 190 200
RDSASDQETS WELQMFKTEN VPESTTLPAV DNNNLFDAVA SPLADPLCDD
210 220 230
IAGNSLPFDN SIDLDNWRNP EAQSGLNSFE LNDFFITS
Note: Induced and active isoform.
Length:238
Mass (Da):26,905
Last modified:October 3, 2006 - v2
Checksum:i81C01ACB3E2531B1
GO
Isoform U (identifier: P41546-1) [UniParc]FASTAAdd to basket

Also known as: Uninduced

The sequence of this isoform differs from the canonical sequence as follows:
     221-238: EAQSGLNSFELNDFFITS → AVITMTRKLQ

Note: Not translated. The unspliced HAC1 mRNA is stable, located in the cytoplasm, and is associated with polyribosomes, yet does not produce protein. Translational attenuation is due to basepairing of the intron with the 5'-UTR of the mRNA.
Show »
Length:230
Mass (Da):26,046
Checksum:iD6CFD739C133AB4B
GO

Sequence cautioni

The sequence BAA05513.1 differs from that shown. Reason: Frameshift at positions 143, 151, 165 and 182. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811D → Y in BAA05513 (PubMed:7816617).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei221 – 23818EAQSG…FFITS → AVITMTRKLQ in isoform U. CuratedVSP_020905Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26506 Genomic DNA. Translation: BAA05513.1. Frameshift.
D50617 Genomic DNA. Translation: BAA24425.1.
D86413 Genomic DNA. Translation: BAA19565.1.
BK006940 Genomic DNA. Translation: DAA12409.1.
PIRiS78571.
RefSeqiNP_116622.1. NM_001179935.1. [P41546-2]

Genome annotation databases

EnsemblFungiiYFL031W; YFL031W; YFL031W. [P41546-2]
GeneIDi850513.
KEGGisce:YFL031W.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26506 Genomic DNA. Translation: BAA05513.1. Frameshift.
D50617 Genomic DNA. Translation: BAA24425.1.
D86413 Genomic DNA. Translation: BAA19565.1.
BK006940 Genomic DNA. Translation: DAA12409.1.
PIRiS78571.
RefSeqiNP_116622.1. NM_001179935.1. [P41546-2]

3D structure databases

ProteinModelPortaliP41546.
SMRiP41546. Positions 47-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31115. 300 interactions.
DIPiDIP-2203N.
IntActiP41546. 3 interactions.
MINTiMINT-4484879.

Proteomic databases

PaxDbiP41546.
PeptideAtlasiP41546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFL031W; YFL031W; YFL031W. [P41546-2]
GeneIDi850513.
KEGGisce:YFL031W.

Organism-specific databases

CYGDiYFL031w.
EuPathDBiFungiDB:YFL031W.
SGDiS000001863. HAC1.

Phylogenomic databases

eggNOGiNOG247265.
HOGENOMiHOG000112845.
InParanoidiP41546.
KOiK16230.
OrthoDBiEOG7PCJV2.

Enzyme and pathway databases

BioCyciYEAST:G3O-30430-MONOMER.

Miscellaneous databases

NextBioi966230.
PROiP41546.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hac1: a novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdc10 mutant of Schizosaccharomyces pombe."
    Nojima H., Leem S.-H., Araki H., Sakai A., Nakashima N., Kanaoka Y., Ono Y.
    Nucleic Acids Res. 22:5279-5288(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Murakami Y.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 192-238.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Signalling from endoplasmic reticulum to nucleus: transcription factor with a basic-leucine zipper motif is required for the unfolded protein-response pathway."
    Mori K., Kawahara T., Yoshida H., Yanagi H., Yura T.
    Genes Cells 1:803-817(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  6. "Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression."
    Nikawa J., Akiyoshi M., Hirata S., Fukuda T.
    Nucleic Acids Res. 24:4222-4226(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response."
    Cox J.S., Walter P.
    Cell 87:391-404(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, ALTERNATIVE SPLICING.
  8. "tRNA ligase is required for regulated mRNA splicing in the unfolded protein response."
    Sidrauski C., Cox J.S., Walter P.
    Cell 87:405-413(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, MRNA LIGATION BY TRL1.
  9. "The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response."
    Sidrauski C., Walter P.
    Cell 90:1031-1039(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, MRNA CLEAVAGE BY IRE1.
  10. "Endoplasmic reticulum stress-induced mRNA splicing permits synthesis of transcription factor Hac1p/Ern4p that activates the unfolded protein response."
    Kawahara T., Yanagi H., Yura T., Mori K.
    Mol. Biol. Cell 8:1845-1862(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, INDUCTION.
  11. "Block of HAC1 mRNA translation by long-range base pairing is released by cytoplasmic splicing upon induction of the unfolded protein response."
    Rueegsegger U., Leber J.H., Walter P.
    Cell 107:103-114(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, INDUCTION.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHAC1_YEAST
AccessioniPrimary (citable) accession number: P41546
Secondary accession number(s): D6VTJ9, P87040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 3, 2006
Last modified: July 22, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.