P41544 (SYS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SYS1

Gene names

Name:	SYS1
Ordered Locus Names:	YJL004C
ORF Names:	J1402, YJR83.17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the targeting of ARL3 to the Golgi. Involved in protein trafficking. May serve as a receptor for acetylated ARL3.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein.
Miscellaneous	Present with 238 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SYS1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from genetic interaction PubMed 8923208. Source: SGD Golgi to plasma membrane protein transport Inferred from mutant phenotype PubMed 16926193. Source: SGD vesicle organization Inferred from physical interaction PubMed 11726510. Source: SGD
Cellular_component	integral to Golgi membrane Inferred from direct assay PubMed 11726510. Source: SGD trans-Golgi network Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Protein SYS1		PRO_0000213942
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 52	23	Helical; Potential
	Topological domain	53 – 78	26	Lumenal Potential
	Transmembrane	79 – 101	23	Helical; Potential
	Topological domain	102 – 105	4	Cytoplasmic Potential
	Transmembrane	106 – 125	20	Helical; Potential
	Topological domain	126 – 131	6	Lumenal Potential
	Transmembrane	132 – 154	23	Helical; Potential
	Topological domain	155 – 203	49	Cytoplasmic Potential
Amino acid modifications
	Modified residue	192	1	Phosphoserine Ref.11
	Cross-link	15		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
Experimental info
	Sequence conflict	149 – 203	55	GTWTT…LESQI → ALT in AAA85159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41544 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 78D398E84713732F	FASTA	203	23,716
	10 20 30 40 50 60 MVSIRRYLRV PNELKPSQIF KQDSLSPSKI GLQIVLLQIF YYTTAIVLFY CWAKLAGYDL 70 80 90 100 110 120 NIKEWLFSWE NIDFTNAYGL SISLLWLLDS LICVFFLTVI VGRSKLAWDF AITIHAINFI 130 140 150 160 170 180 VVFLYTRKFP SFSWFFLQIL SSLILIFLGT WTTRWRELRD TFFEGLVDPN EGEVGLVTPS 190 200 QQHSNHSELE QSPIQLKDLE SQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase." Pathak R., Parker C.S., Imperiali B. FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast adenylate cyclase catalytic domain is carboxy terminal." Masson P., Lenzen G., Jacquemin J.M., Danchin A. Curr. Genet. 10:343-352(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-203.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-15, MASS SPECTROMETRY.
[8]	"Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p." Behnia R., Panic B., Whyte J.R.C., Munro S. Nat. Cell Biol. 6:405-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARL3.
[9]	"Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p." Setty S.R., Strochlic T.I., Tong A.H., Boone C., Burd C.G. Nat. Cell Biol. 6:414-419(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARL3.
[10]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22326 Genomic DNA. Translation: AAA85159.1. Z49279 Genomic DNA. Translation: CAA89294.1. X87611 Genomic DNA. Translation: CAA60918.1. AY558345 Genomic DNA. Translation: AAS56671.1. X03449 Genomic DNA. Translation: CAA27176.1. BK006943 Genomic DNA. Translation: DAA08787.1.
PIR	S55184.
RefSeq	NP_012530.1. NM_001181438.1.
3D structure databases
ProteinModelPortal	P41544.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2613N.
IntAct	P41544. 36 interactions.
MINT	MINT-423473.
STRING	4932.YJL004C.
Proteomic databases
PaxDb	P41544.
Protocols and materials databases
DNASU	853453.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL004C; YJL004C; YJL004C.
GeneID	853453.
KEGG	sce:YJL004C.
Organism-specific databases
CYGD	YJL004c.
SGD	S000003541. SYS1.
Phylogenomic databases
eggNOG	NOG237580.
HOGENOM	HOG000000813.
OMA	DFAITIH.
OrthoDB	EOG4V72RZ.
Gene expression databases
Genevestigator	P41544.
GermOnline	YJL004C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019185. Integral_membrane_SYS1-rel. [Graphical view]
Pfam	PF09801. SYS1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974017.

Entry information

Entry name

SYS1_YEAST

Accession

Primary (citable) accession number: P41544
Secondary accession number(s): D6VWH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program