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P41544 (SYS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SYS1
Gene names
Name:SYS1
Ordered Locus Names:YJL004C
ORF Names:J1402, YJR83.17
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for the targeting of ARL3 to the Golgi. Involved in protein trafficking. May serve as a receptor for acetylated ARL3.

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein.

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SYS1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203Protein SYS1
PRO_0000213942

Regions

Topological domain1 – 2929Cytoplasmic Potential
Transmembrane30 – 5223Helical; Potential
Topological domain53 – 7826Lumenal Potential
Transmembrane79 – 10123Helical; Potential
Topological domain102 – 1054Cytoplasmic Potential
Transmembrane106 – 12520Helical; Potential
Topological domain126 – 1316Lumenal Potential
Transmembrane132 – 15423Helical; Potential
Topological domain155 – 20349Cytoplasmic Potential

Amino acid modifications

Modified residue1921Phosphoserine Ref.11
Cross-link15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Experimental info

Sequence conflict149 – 20355GTWTT…LESQI → ALT in AAA85159. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41544 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 78D398E84713732F

FASTA20323,716
        10         20         30         40         50         60 
MVSIRRYLRV PNELKPSQIF KQDSLSPSKI GLQIVLLQIF YYTTAIVLFY CWAKLAGYDL 

        70         80         90        100        110        120 
NIKEWLFSWE NIDFTNAYGL SISLLWLLDS LICVFFLTVI VGRSKLAWDF AITIHAINFI 

       130        140        150        160        170        180 
VVFLYTRKFP SFSWFFLQIL SSLILIFLGT WTTRWRELRD TFFEGLVDPN EGEVGLVTPS 

       190        200 
QQHSNHSELE QSPIQLKDLE SQI 

« Hide

References

« Hide 'large scale' references
[1]"The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
Pathak R., Parker C.S., Imperiali B.
FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Yeast adenylate cyclase catalytic domain is carboxy terminal."
Masson P., Lenzen G., Jacquemin J.M., Danchin A.
Curr. Genet. 10:343-352(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-203.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-15.
[8]"Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p."
Behnia R., Panic B., Whyte J.R.C., Munro S.
Nat. Cell Biol. 6:405-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL3.
[9]"Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p."
Setty S.R., Strochlic T.I., Tong A.H., Boone C., Burd C.G.
Nat. Cell Biol. 6:414-419(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL3.
[10]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22326 Genomic DNA. Translation: AAA85159.1.
Z49279 Genomic DNA. Translation: CAA89294.1.
X87611 Genomic DNA. Translation: CAA60918.1.
AY558345 Genomic DNA. Translation: AAS56671.1.
X03449 Genomic DNA. Translation: CAA27176.1.
BK006943 Genomic DNA. Translation: DAA08787.1.
PIRS55184.
RefSeqNP_012530.1. NM_001181438.1.

3D structure databases

ProteinModelPortalP41544.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33753. 225 interactions.
DIPDIP-2613N.
IntActP41544. 39 interactions.
MINTMINT-423473.
STRING4932.YJL004C.

Proteomic databases

PaxDbP41544.

Protocols and materials databases

DNASU853453.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL004C; YJL004C; YJL004C.
GeneID853453.
KEGGsce:YJL004C.

Organism-specific databases

CYGDYJL004c.
SGDS000003541. SYS1.

Phylogenomic databases

eggNOGNOG237580.
HOGENOMHOG000000813.
OMAYSTRWKE.
OrthoDBEOG72G1KR.

Enzyme and pathway databases

BioCycYEAST:G3O-31483-MONOMER.

Gene expression databases

GenevestigatorP41544.

Family and domain databases

InterProIPR019185. Integral_membrane_SYS1-rel.
[Graphical view]
PfamPF09801. SYS1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974017.
PROP41544.

Entry information

Entry nameSYS1_YEAST
AccessionPrimary (citable) accession number: P41544
Secondary accession number(s): D6VWH1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families