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P41544

- SYS1_YEAST

UniProt

P41544 - SYS1_YEAST

Protein

Protein SYS1

Gene

SYS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Necessary for the targeting of ARL3 to the Golgi. Involved in protein trafficking. May serve as a receptor for acetylated ARL3.

    GO - Biological processi

    1. Golgi to endosome transport Source: SGD
    2. Golgi to plasma membrane protein transport Source: SGD
    3. protein targeting to Golgi Source: SGD
    4. vesicle organization Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31483-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SYS1
    Gene namesi
    Name:SYS1
    Ordered Locus Names:YJL004C
    ORF Names:J1402, YJR83.17
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL004c.
    SGDiS000003541. SYS1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of Golgi membrane Source: SGD
    2. trans-Golgi network Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 203203Protein SYS1PRO_0000213942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki15 – 15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei192 – 1921Phosphoserine1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP41544.

    Expressioni

    Gene expression databases

    GenevestigatoriP41544.

    Interactioni

    Protein-protein interaction databases

    BioGridi33753. 225 interactions.
    DIPiDIP-2613N.
    IntActiP41544. 39 interactions.
    MINTiMINT-423473.
    STRINGi4932.YJL004C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41544.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini53 – 7826LumenalSequence AnalysisAdd
    BLAST
    Topological domaini102 – 1054CytoplasmicSequence Analysis
    Topological domaini126 – 1316LumenalSequence Analysis
    Topological domaini155 – 20349CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei79 – 10123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei106 – 12520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei132 – 15423HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SYS1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG237580.
    HOGENOMiHOG000000813.
    OMAiYSTRWKE.
    OrthoDBiEOG72G1KR.

    Family and domain databases

    InterProiIPR019185. Integral_membrane_SYS1-rel.
    [Graphical view]
    PfamiPF09801. SYS1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41544-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSIRRYLRV PNELKPSQIF KQDSLSPSKI GLQIVLLQIF YYTTAIVLFY    50
    CWAKLAGYDL NIKEWLFSWE NIDFTNAYGL SISLLWLLDS LICVFFLTVI 100
    VGRSKLAWDF AITIHAINFI VVFLYTRKFP SFSWFFLQIL SSLILIFLGT 150
    WTTRWRELRD TFFEGLVDPN EGEVGLVTPS QQHSNHSELE QSPIQLKDLE 200
    SQI 203
    Length:203
    Mass (Da):23,716
    Last modified:November 1, 1995 - v1
    Checksum:i78D398E84713732F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti149 – 20355GTWTT…LESQI → ALT in AAA85159. (PubMed:7720878)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22326 Genomic DNA. Translation: AAA85159.1.
    Z49279 Genomic DNA. Translation: CAA89294.1.
    X87611 Genomic DNA. Translation: CAA60918.1.
    AY558345 Genomic DNA. Translation: AAS56671.1.
    X03449 Genomic DNA. Translation: CAA27176.1.
    BK006943 Genomic DNA. Translation: DAA08787.1.
    PIRiS55184.
    RefSeqiNP_012530.1. NM_001181438.1.

    Genome annotation databases

    EnsemblFungiiYJL004C; YJL004C; YJL004C.
    GeneIDi853453.
    KEGGisce:YJL004C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22326 Genomic DNA. Translation: AAA85159.1 .
    Z49279 Genomic DNA. Translation: CAA89294.1 .
    X87611 Genomic DNA. Translation: CAA60918.1 .
    AY558345 Genomic DNA. Translation: AAS56671.1 .
    X03449 Genomic DNA. Translation: CAA27176.1 .
    BK006943 Genomic DNA. Translation: DAA08787.1 .
    PIRi S55184.
    RefSeqi NP_012530.1. NM_001181438.1.

    3D structure databases

    ProteinModelPortali P41544.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33753. 225 interactions.
    DIPi DIP-2613N.
    IntActi P41544. 39 interactions.
    MINTi MINT-423473.
    STRINGi 4932.YJL004C.

    Proteomic databases

    PaxDbi P41544.

    Protocols and materials databases

    DNASUi 853453.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL004C ; YJL004C ; YJL004C .
    GeneIDi 853453.
    KEGGi sce:YJL004C.

    Organism-specific databases

    CYGDi YJL004c.
    SGDi S000003541. SYS1.

    Phylogenomic databases

    eggNOGi NOG237580.
    HOGENOMi HOG000000813.
    OMAi YSTRWKE.
    OrthoDBi EOG72G1KR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31483-MONOMER.

    Miscellaneous databases

    NextBioi 974017.
    PROi P41544.

    Gene expression databases

    Genevestigatori P41544.

    Family and domain databases

    InterProi IPR019185. Integral_membrane_SYS1-rel.
    [Graphical view ]
    Pfami PF09801. SYS1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
      Pathak R., Parker C.S., Imperiali B.
      FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Yeast adenylate cyclase catalytic domain is carboxy terminal."
      Masson P., Lenzen G., Jacquemin J.M., Danchin A.
      Curr. Genet. 10:343-352(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-203.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
      Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
      Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-15.
    8. "Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p."
      Behnia R., Panic B., Whyte J.R.C., Munro S.
      Nat. Cell Biol. 6:405-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARL3.
    9. "Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p."
      Setty S.R., Strochlic T.I., Tong A.H., Boone C., Burd C.G.
      Nat. Cell Biol. 6:414-419(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARL3.
    10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYS1_YEAST
    AccessioniPrimary (citable) accession number: P41544
    Secondary accession number(s): D6VWH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 238 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3