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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

OST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: SGD

GO - Biological processi

  • protein N-linked glycosylation Source: SGD
  • protein N-linked glycosylation via asparagine Source: SGD

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YJL002C-MONOMER
YEAST:YJL002C-MONOMER
BRENDAi2.4.99.18 984
UniPathwayiUPA00378

Protein family/group databases

TCDBi9.B.142.3.14 the integral membrane glycosyltransferase family 39 (gt39) family

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (EC:2.4.99.18)
Alternative name(s):
Oligosaccharyl transferase 64 kDa subunit
Oligosaccharyl transferase subunit OST1
Oligosaccharyl transferase subunit alpha
Gene namesi
Name:OST1
Synonyms:NLT1
Ordered Locus Names:YJL002C
ORF Names:J1404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL002C
SGDiS000003539 OST1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 449LumenalSequence analysisAdd BLAST427
Transmembranei450 – 470HelicalSequence analysisAdd BLAST21
Topological domaini471 – 476CytoplasmicSequence analysis6

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002196023 – 476Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi18N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi336N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi400N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP41543
PaxDbiP41543
PRIDEiP41543

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33755, 189 interactors
DIPiDIP-2454N
IntActiP41543, 27 interactors
MINTiP41543
STRINGi4932.YJL002C

Chemistry databases

BindingDBiP41543

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 41Combined sources10
Beta strandi43 – 46Combined sources4
Beta strandi48 – 56Combined sources9
Beta strandi59 – 61Combined sources3
Beta strandi66 – 70Combined sources5
Turni72 – 76Combined sources5
Beta strandi77 – 84Combined sources8
Beta strandi86 – 88Combined sources3
Beta strandi93 – 96Combined sources4
Beta strandi113 – 118Combined sources6
Beta strandi128 – 138Combined sources11
Beta strandi142 – 147Combined sources6
Beta strandi150 – 152Combined sources3
Beta strandi155 – 163Combined sources9
Beta strandi170 – 172Combined sources3
Beta strandi174 – 181Combined sources8
Beta strandi183 – 187Combined sources5
Beta strandi192 – 194Combined sources3
Beta strandi197 – 199Combined sources3
Beta strandi202 – 205Combined sources4
Beta strandi209 – 211Combined sources3
Beta strandi219 – 225Combined sources7
Beta strandi231 – 243Combined sources13
Turni244 – 247Combined sources4
Beta strandi248 – 259Combined sources12
Helixi270 – 279Combined sources10
Beta strandi286 – 289Combined sources4
Beta strandi291 – 294Combined sources4
Beta strandi303 – 305Combined sources3
Beta strandi307 – 310Combined sources4
Beta strandi314 – 317Combined sources4
Beta strandi320 – 323Combined sources4
Beta strandi334 – 344Combined sources11
Helixi345 – 347Combined sources3
Beta strandi349 – 351Combined sources3
Beta strandi358 – 365Combined sources8
Beta strandi370 – 375Combined sources6
Beta strandi377 – 383Combined sources7
Beta strandi388 – 393Combined sources6
Beta strandi399 – 406Combined sources8
Beta strandi416 – 423Combined sources8
Beta strandi425 – 427Combined sources3
Turni428 – 430Combined sources3
Beta strandi434 – 440Combined sources7
Helixi443 – 446Combined sources4
Helixi448 – 466Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6C26electron microscopy3.5011-476[»]
6EZNelectron microscopy3.30A1-476[»]
ProteinModelPortaliP41543
SMRiP41543
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000009630
HOGENOMiHOG000093925
InParanoidiP41543
KOiK12666
OMAiRYPIFGG
OrthoDBiEOG092C29MC

Family and domain databases

InterProiView protein in InterPro
IPR007676 Ribophorin_I
PANTHERiPTHR21049 PTHR21049, 1 hit
PfamiView protein in Pfam
PF04597 Ribophorin_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET
60 70 80 90 100
IEITIKNIAS EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS
110 120 130 140 150
TTAPGDDGES EIRYGIIQFP NAISPQEEVS LVIKSFYNTV GIPYPEHVGM
160 170 180 190 200
SEEQHLLWET NRLPLSAYDT KKASFTLIGS SSFEEYHPPN DESLLGKANG
210 220 230 240 250
NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI WLSHWASTIQ
260 270 280 290 300
FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT
310 320 330 340 350
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH
360 370 380 390 400
VSSGSDEKFV ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN
410 420 430 440 450
VSIETQKSYF DLNKGHVKLT FSYRNLISQV ANGQVLIKYD YPKSSFFKKP
460 470
LSIACYIFTA LMGVFVLKTL NMNVTN
Length:476
Mass (Da):54,072
Last modified:November 1, 1995 - v1
Checksum:iEFD989B50AAED348
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46719 Genomic DNA Translation: CAA86674.1
U22326 Genomic DNA Translation: AAA85158.1
X87611 Genomic DNA Translation: CAA60920.1
Z49277 Genomic DNA Translation: CAA89291.1
BK006943 Genomic DNA Translation: DAA08789.1
PIRiA56510
RefSeqiNP_012532.3, NM_001181436.3

Genome annotation databases

EnsemblFungiiYJL002C; YJL002C; YJL002C
GeneIDi853455
KEGGisce:YJL002C

Entry informationi

Entry nameiOST1_YEAST
AccessioniPrimary (citable) accession number: P41543
Secondary accession number(s): D6VWH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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