Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P41543 (OST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

EC=2.4.99.18
Alternative name(s):
Oligosaccharyl transferase 64 kDa subunit
Oligosaccharyl transferase subunit OST1
Oligosaccharyl transferase subunit alpha
Gene names
Name:OST1
Synonyms:NLT1
Ordered Locus Names:YJL002C
ORF Names:J1404
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activity

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1. Ref.5 Ref.6 Ref.12 Ref.13

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the OST1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-12651,EBI-12651
SSS1P351792EBI-12651,EBI-16406

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1
Chain23 – 476454Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
PRO_0000021960

Regions

Topological domain23 – 449427Lumenal Potential
Transmembrane450 – 47021Helical; Potential
Topological domain471 – 4766Cytoplasmic Potential

Amino acid modifications

Glycosylation181N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P41543 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: EFD989B50AAED348

FASTA47654,072
        10         20         30         40         50         60 
MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET IEITIKNIAS 

        70         80         90        100        110        120 
EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS TTAPGDDGES EIRYGIIQFP 

       130        140        150        160        170        180 
NAISPQEEVS LVIKSFYNTV GIPYPEHVGM SEEQHLLWET NRLPLSAYDT KKASFTLIGS 

       190        200        210        220        230        240 
SSFEEYHPPN DESLLGKANG NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI 

       250        260        270        280        290        300 
WLSHWASTIQ FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 

       310        320        330        340        350        360 
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH VSSGSDEKFV 

       370        380        390        400        410        420 
ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN VSIETQKSYF DLNKGHVKLT 

       430        440        450        460        470 
FSYRNLISQV ANGQVLIKYD YPKSSFFKKP LSIACYIFTA LMGVFVLKTL NMNVTN 

« Hide

References

« Hide 'large scale' references
[1]"The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I."
Silberstein S., Collins P.G., Kelleher D.J., Rapiejko P.J., Gilmore R.
J. Cell Biol. 128:525-536(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-38 AND 135-165.
[2]"The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
Pathak R., Parker C.S., Imperiali B.
FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
Kelleher D.J., Gilmore R.
J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[6]"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
Karaoglu D., Kelleher D.J., Gilmore R.
J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[7]"The oligosaccharyltransferase complex from yeast."
Knauer R., Lehle L.
Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
Schwarz M., Knauer R., Lehle L.
FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[11]"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
Yan A., Lennarz W.J.
Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[12]"Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
Chavan M., Yan A., Lennarz W.J.
J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
[13]"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
Yan A., Wu E., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46719 Genomic DNA. Translation: CAA86674.1.
U22326 Genomic DNA. Translation: AAA85158.1.
X87611 Genomic DNA. Translation: CAA60920.1.
Z49277 Genomic DNA. Translation: CAA89291.1.
BK006943 Genomic DNA. Translation: DAA08789.1.
PIRA56510.
RefSeqNP_012532.3. NM_001181436.3.

3D structure databases

ProteinModelPortalP41543.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33755. 79 interactions.
DIPDIP-2454N.
IntActP41543. 22 interactions.
MINTMINT-473095.
STRING4932.YJL002C.

Chemistry

BindingDBP41543.

Proteomic databases

PaxDbP41543.
PeptideAtlasP41543.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL002C; YJL002C; YJL002C.
GeneID853455.
KEGGsce:YJL002C.

Organism-specific databases

CYGDYJL002c.
SGDS000003539. OST1.

Phylogenomic databases

eggNOGNOG292474.
GeneTreeENSGT00390000009630.
HOGENOMHOG000093925.
KOK12666.
OMAWDGLRKP.
OrthoDBEOG7W9S43.

Enzyme and pathway databases

BioCycYEAST:YJL002C-MONOMER.
BRENDA2.4.1.119. 984.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP41543.

Family and domain databases

InterProIPR007676. Ribophorin_I.
[Graphical view]
PANTHERPTHR21049. PTHR21049. 1 hit.
PfamPF04597. Ribophorin_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974023.
PROP41543.

Entry information

Entry nameOST1_YEAST
AccessionPrimary (citable) accession number: P41543
Secondary accession number(s): D6VWH3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways