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Reviewed, UniProtKB/Swiss-Prot P41543 (OST1_YEAST)

Last modified January 19, 2010. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
    EC=2.4.1.119
Alternative name(s):
    Oligosaccharyl transferase subunit OST1
    Oligosaccharyl transferase subunit alpha
    Oligosaccharyl transferase 64 kDa subunit
Gene names
Name: OST1
Synonyms: NLT1
Ordered Locus Names: YJL002C
ORF Names: J1404
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Component of the oligosaccharyl transferase (OST) complex, which apears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1. Ref.11 Ref.12

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the OST1 family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-12651,EBI-12651
P360761EBI-12651,EBI-26778
P402071EBI-12651,EBI-27272
P436131EBI-12651,EBI-23020
P538721EBI-12651,EBI-29075
AKR1P390101EBI-12651,EBI-2421
ALG11P539541EBI-12651,EBI-2497
ARF1P110761EBI-12651,EBI-2816
ATG27P469891EBI-12651,EBI-26164
BAP2P380841EBI-12651,EBI-3414
CAN1P048171EBI-12651,EBI-3993
CBR1P386261EBI-12651,EBI-2048224
CCW12Q121271EBI-12651,EBI-5303
CHO1P084561EBI-12651,EBI-14055
CHS7P388431EBI-12651,EBI-24756
COS1P538221EBI-12651,EBI-4959
COS2P383631EBI-12651,EBI-4963
COS4P435421EBI-12651,EBI-2050225
COS8P387231EBI-12651,EBI-4985
CPR4P253341EBI-12651,EBI-5468
CPT1P178981EBI-12651,EBI-2050738
CSG2P352061EBI-12651,EBI-2051140
CTR2P388651EBI-12651,EBI-5268
DAL5P153651EBI-12651,EBI-5527
DCW1P360911EBI-12651,EBI-2052781
DFG5Q050311EBI-12651,EBI-27512
DIP5P533881EBI-12651,EBI-5904
DPP1Q055211EBI-12651,EBI-2054360
EMP47P435551EBI-12651,EBI-6439
EPT1P221401EBI-12651,EBI-6494
ERG11P106141EBI-12651,EBI-5127
ERG24P324621EBI-12651,EBI-6502
ERG25P530451EBI-12651,EBI-6506
ERG26P531991EBI-12651,EBI-6514
ERG3P323531EBI-12651,EBI-6554
ERG4P253401EBI-12651,EBI-6558
ERG5P547811EBI-12651,EBI-6563
ERP1Q053591EBI-12651,EBI-6581
ERP4Q124501EBI-12651,EBI-6598
ERP5P388191EBI-12651,EBI-6603
ERP6P531981EBI-12651,EBI-2058104
ERV25P548371EBI-12651,EBI-6642
ERV29P533371EBI-12651,EBI-23662
ESBP6P539181EBI-12651,EBI-28895
FAA3P390021EBI-12651,EBI-10089
FET3P389931EBI-12651,EBI-6876
FET5P435611EBI-12651,EBI-6891
GAP1P191451EBI-12651,EBI-7314
GNP1P488131EBI-12651,EBI-7758
GOT1Q035541EBI-12651,EBI-2057821
GPT2P361481EBI-12651,EBI-26471
GTT1P405821EBI-12651,EBI-7941
HIP1P067751EBI-12651,EBI-8308
ILM1P471551EBI-12651,EBI-2051644
ITR1P306051EBI-12651,EBI-2050956
KRE1P172601EBI-12651,EBI-2068892
KRE27P405401EBI-12651,EBI-24977
LAC1P284961EBI-12651,EBI-26585
MSC7P386941EBI-12651,EBI-24511
NKP2Q061621EBI-12651,EBI-34256
OLE1P211471EBI-12651,EBI-2098
ORM2Q061441EBI-12651,EBI-34916
OSH1P358451EBI-12651,EBI-12611
OSH2Q124511EBI-12651,EBI-12621
OSH7P387551EBI-12651,EBI-12641
OST6Q037231EBI-12651,EBI-12696
PEM1P053741EBI-12651,EBI-2049142
PHO84P252971EBI-12651,EBI-13320
PHO86P469561EBI-12651,EBI-13337
PHO88P382641EBI-12651,EBI-13350
PIS1P061971EBI-12651,EBI-13458
PKR1Q038801EBI-12651,EBI-27252
PPZ1P265701EBI-12651,EBI-13807
PRM8P531741EBI-12651,EBI-6200
QDR2P404741EBI-12651,EBI-25203
SAC1P323681EBI-12651,EBI-16210
SBH1P528701EBI-12651,EBI-16410
SCS7Q035291EBI-12651,EBI-16747
SEC4P075601EBI-12651,EBI-16858
SEC61P329152EBI-12651,EBI-16400
SEY1Q992871EBI-12651,EBI-37523
SHR3Q027741EBI-12651,EBI-17099
SIS2P360241EBI-12651,EBI-17250
SLC1P333331EBI-12651,EBI-13494
SNL1P405481EBI-12651,EBI-24950
SNQ2P325681EBI-12651,EBI-17590
SPC1P469651EBI-12651,EBI-17823
SPC2Q049691EBI-12651,EBI-27827
SPF1P399861EBI-12651,EBI-3128
SSH1P383531EBI-12651,EBI-18175
SSS1P351791EBI-12651,EBI-16406
STE24P471541EBI-12651,EBI-18298
SUR2P389921EBI-12651,EBI-18574
SWP1Q027951EBI-12651,EBI-12666
TGL4P361651EBI-12651,EBI-26531
UIP4Q089261EBI-12651,EBI-2044051
VHS3Q084381EBI-12651,EBI-30482
VMA11P328421EBI-12651,EBI-20311
VMA21P418061EBI-12651,EBI-20323
WBP1P337671EBI-12651,EBI-12658
YDC1Q028961EBI-12651,EBI-22203
YOP1Q124021EBI-12651,EBI-37092
YPC1P382981EBI-12651,EBI-2103141
YPS3Q123031EBI-12651,EBI-27104
YPT1P011231EBI-12651,EBI-29496

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1
Chain23 – 476454Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
PRO_0000021960

Regions

Topological domain23 – 449427Lumenal Potential
Transmembrane450 – 47021 Potential
Topological domain471 – 4766Cytoplasmic Potential

Amino acid modifications

Glycosylation181N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P41543-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: EFD989B50AAED348

FASTA47654,072
        10         20         30         40         50         60 
MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET IEITIKNIAS 

        70         80         90        100        110        120 
EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS TTAPGDDGES EIRYGIIQFP 

       130        140        150        160        170        180 
NAISPQEEVS LVIKSFYNTV GIPYPEHVGM SEEQHLLWET NRLPLSAYDT KKASFTLIGS 

       190        200        210        220        230        240 
SSFEEYHPPN DESLLGKANG NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI 

       250        260        270        280        290        300 
WLSHWASTIQ FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 

       310        320        330        340        350        360 
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH VSSGSDEKFV 

       370        380        390        400        410        420 
ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN VSIETQKSYF DLNKGHVKLT 

       430        440        450        460        470 
FSYRNLISQV ANGQVLIKYD YPKSSFFKKP LSIACYIFTA LMGVFVLKTL NMNVTN

« Hide

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References

« Hide 'large scale' references
[1]"The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I."
Silberstein S., Collins P.G., Kelleher D.J., Rapiejko P.J., Gilmore R.
J. Cell Biol. 128:525-536(1995) [PubMed: 7860628] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-38 AND 135-165.
[2]"The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
Pathak R., Parker C.S., Imperiali B.
FEBS Lett. 362:229-234(1995) [PubMed: 7720878] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
Kelleher D.J., Gilmore R.
J. Biol. Chem. 269:12908-12917(1994) [PubMed: 8175708] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[5]"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
Karaoglu D., Kelleher D.J., Gilmore R.
J. Biol. Chem. 272:32513-32520(1997) [PubMed: 9405463] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[6]"The oligosaccharyltransferase complex from yeast."
Knauer R., Lehle L.
Biochim. Biophys. Acta 1426:259-273(1999) [PubMed: 9878773] [Abstract]
Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
Schwarz M., Knauer R., Lehle L.
FEBS Lett. 579:6564-6568(2005) [PubMed: 16297388] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[9]"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
Glycobiology 15:1396-1406(2005) [PubMed: 16096346] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
Yan A., Lennarz W.J.
Glycobiology 15:1407-1415(2005) [PubMed: 16096345] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[11]"Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
Chavan M., Yan A., Lennarz W.J.
J. Biol. Chem. 280:22917-22924(2005) [PubMed: 15831493] [Abstract]
Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
[12]"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
Yan A., Wu E., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed: 15886282] [Abstract]
Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z46719 Genomic DNA. Translation: CAA86674.1.
U22326 Genomic DNA. Translation: AAA85158.1.
X87611 Genomic DNA. Translation: CAA60920.1.
Z49277 Genomic DNA. Translation: CAA89291.1.
PIRA56510.
RefSeqNP_012532.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2454N.
IntActP41543. 135 interactions.
STRINGP41543.

Proteomic databases

PeptideAtlasP41543.

Genome annotation databases

EnsemblYJL002C; YJL002C; YJL002C; Saccharomyces cerevisiae. [Genome view]
GeneID853455.
KEGGsce:YJL002C.
NMPDRfig|4932.3.peg.3505.

Organism-specific databases

CYGDYJL002c.
SGDS000003539. OST1.

Phylogenomic databases

eggNOGfuNOG05907.
HOGENOMHBG396766.
OMAFLNITFG.
OrthoDBEOG9FBK99.
PhylomeDBP41543.

Enzyme and pathway databases

BRENDA2.4.1.119. 250.

Gene expression databases

ArrayExpressP41543.
GenevestigatorP41543.
GermOnlineYJL002C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR007676. Ribophorin_I.
[Graphical view]
PANTHERPTHR21049. Ribophorin_I. 1 hit.
PfamPF04597. Ribophorin_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974023.

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Entry information

Entry nameOST1_YEAST
AccessionPrimary (citable) accession number: P41543
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 19, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents