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P41543

- OST1_YEAST

UniProt

P41543 - OST1_YEAST

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Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Gene
OST1, NLT1, YJL002C, J1404
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: SGD
  2. identical protein binding Source: IntAct
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. protein N-linked glycosylation Source: SGD
  2. protein N-linked glycosylation via asparagine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YJL002C-MONOMER.
BRENDAi2.4.1.119. 984.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (EC:2.4.99.18)
Alternative name(s):
Oligosaccharyl transferase 64 kDa subunit
Oligosaccharyl transferase subunit OST1
Oligosaccharyl transferase subunit alpha
Gene namesi
Name:OST1
Synonyms:NLT1
Ordered Locus Names:YJL002C
ORF Names:J1404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL002c.
SGDiS000003539. OST1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 449427Lumenal Reviewed prediction
Add
BLAST
Transmembranei450 – 47021Helical; Reviewed prediction
Add
BLAST
Topological domaini471 – 4766Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. oligosaccharyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 Publication
Add
BLAST
Chaini23 – 476454Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
PRO_0000021960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi18 – 181N-linked (GlcNAc...) Reviewed prediction
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi217 – 2171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi336 – 3361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi400 – 4001N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP41543.
PaxDbiP41543.
PeptideAtlasiP41543.

Expressioni

Gene expression databases

GenevestigatoriP41543.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-12651,EBI-12651
SSS1P351792EBI-12651,EBI-16406

Protein-protein interaction databases

BioGridi33755. 79 interactions.
DIPiDIP-2454N.
IntActiP41543. 22 interactions.
MINTiMINT-473095.
STRINGi4932.YJL002C.

Structurei

3D structure databases

ProteinModelPortaliP41543.

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292474.
GeneTreeiENSGT00390000009630.
HOGENOMiHOG000093925.
KOiK12666.
OMAiFRIGWND.
OrthoDBiEOG7W9S43.

Family and domain databases

InterProiIPR007676. Ribophorin_I.
[Graphical view]
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiPF04597. Ribophorin_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41543-1 [UniParc]FASTAAdd to Basket

« Hide

MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET    50
IEITIKNIAS EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS 100
TTAPGDDGES EIRYGIIQFP NAISPQEEVS LVIKSFYNTV GIPYPEHVGM 150
SEEQHLLWET NRLPLSAYDT KKASFTLIGS SSFEEYHPPN DESLLGKANG 200
NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI WLSHWASTIQ 250
FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 300
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH 350
VSSGSDEKFV ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN 400
VSIETQKSYF DLNKGHVKLT FSYRNLISQV ANGQVLIKYD YPKSSFFKKP 450
LSIACYIFTA LMGVFVLKTL NMNVTN 476
Length:476
Mass (Da):54,072
Last modified:November 1, 1995 - v1
Checksum:iEFD989B50AAED348
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46719 Genomic DNA. Translation: CAA86674.1.
U22326 Genomic DNA. Translation: AAA85158.1.
X87611 Genomic DNA. Translation: CAA60920.1.
Z49277 Genomic DNA. Translation: CAA89291.1.
BK006943 Genomic DNA. Translation: DAA08789.1.
PIRiA56510.
RefSeqiNP_012532.3. NM_001181436.3.

Genome annotation databases

EnsemblFungiiYJL002C; YJL002C; YJL002C.
GeneIDi853455.
KEGGisce:YJL002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46719 Genomic DNA. Translation: CAA86674.1 .
U22326 Genomic DNA. Translation: AAA85158.1 .
X87611 Genomic DNA. Translation: CAA60920.1 .
Z49277 Genomic DNA. Translation: CAA89291.1 .
BK006943 Genomic DNA. Translation: DAA08789.1 .
PIRi A56510.
RefSeqi NP_012532.3. NM_001181436.3.

3D structure databases

ProteinModelPortali P41543.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33755. 79 interactions.
DIPi DIP-2454N.
IntActi P41543. 22 interactions.
MINTi MINT-473095.
STRINGi 4932.YJL002C.

Chemistry

BindingDBi P41543.

Proteomic databases

MaxQBi P41543.
PaxDbi P41543.
PeptideAtlasi P41543.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL002C ; YJL002C ; YJL002C .
GeneIDi 853455.
KEGGi sce:YJL002C.

Organism-specific databases

CYGDi YJL002c.
SGDi S000003539. OST1.

Phylogenomic databases

eggNOGi NOG292474.
GeneTreei ENSGT00390000009630.
HOGENOMi HOG000093925.
KOi K12666.
OMAi FRIGWND.
OrthoDBi EOG7W9S43.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:YJL002C-MONOMER.
BRENDAi 2.4.1.119. 984.

Miscellaneous databases

NextBioi 974023.
PROi P41543.

Gene expression databases

Genevestigatori P41543.

Family and domain databases

InterProi IPR007676. Ribophorin_I.
[Graphical view ]
PANTHERi PTHR21049. PTHR21049. 1 hit.
Pfami PF04597. Ribophorin_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I."
    Silberstein S., Collins P.G., Kelleher D.J., Rapiejko P.J., Gilmore R.
    J. Cell Biol. 128:525-536(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-38 AND 135-165.
  2. "The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
    Pathak R., Parker C.S., Imperiali B.
    FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
    Kelleher D.J., Gilmore R.
    J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
    Karaoglu D., Kelleher D.J., Gilmore R.
    J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  7. "The oligosaccharyltransferase complex from yeast."
    Knauer R., Lehle L.
    Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
    Schwarz M., Knauer R., Lehle L.
    FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
    Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
    Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
    Yan A., Lennarz W.J.
    Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
    Chavan M., Yan A., Lennarz W.J.
    J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
  13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
    Yan A., Wu E., Lennarz W.J.
    Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.

Entry informationi

Entry nameiOST1_YEAST
AccessioniPrimary (citable) accession number: P41543
Secondary accession number(s): D6VWH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11900 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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