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P41543

- OST1_YEAST

UniProt

P41543 - OST1_YEAST

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

OST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: SGD
    2. identical protein binding Source: IntAct
    3. protein binding Source: IntAct

    GO - Biological processi

    1. protein N-linked glycosylation Source: SGD
    2. protein N-linked glycosylation via asparagine Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YJL002C-MONOMER.
    BRENDAi2.4.1.119. 984.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (EC:2.4.99.18)
    Alternative name(s):
    Oligosaccharyl transferase 64 kDa subunit
    Oligosaccharyl transferase subunit OST1
    Oligosaccharyl transferase subunit alpha
    Gene namesi
    Name:OST1
    Synonyms:NLT1
    Ordered Locus Names:YJL002C
    ORF Names:J1404
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL002c.
    SGDiS000003539. OST1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. oligosaccharyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22221 PublicationAdd
    BLAST
    Chaini23 – 476454Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1PRO_0000021960Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi18 – 181N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP41543.
    PaxDbiP41543.
    PeptideAtlasiP41543.

    Expressioni

    Gene expression databases

    GenevestigatoriP41543.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-12651,EBI-12651
    SSS1P351792EBI-12651,EBI-16406

    Protein-protein interaction databases

    BioGridi33755. 81 interactions.
    DIPiDIP-2454N.
    IntActiP41543. 22 interactions.
    MINTiMINT-473095.
    STRINGi4932.YJL002C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41543.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 449427LumenalSequence AnalysisAdd
    BLAST
    Topological domaini471 – 4766CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei450 – 47021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the OST1 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG292474.
    GeneTreeiENSGT00390000009630.
    HOGENOMiHOG000093925.
    KOiK12666.
    OMAiFRIGWND.
    OrthoDBiEOG7W9S43.

    Family and domain databases

    InterProiIPR007676. Ribophorin_I.
    [Graphical view]
    PANTHERiPTHR21049. PTHR21049. 1 hit.
    PfamiPF04597. Ribophorin_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41543-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET    50
    IEITIKNIAS EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS 100
    TTAPGDDGES EIRYGIIQFP NAISPQEEVS LVIKSFYNTV GIPYPEHVGM 150
    SEEQHLLWET NRLPLSAYDT KKASFTLIGS SSFEEYHPPN DESLLGKANG 200
    NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI WLSHWASTIQ 250
    FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 300
    DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH 350
    VSSGSDEKFV ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN 400
    VSIETQKSYF DLNKGHVKLT FSYRNLISQV ANGQVLIKYD YPKSSFFKKP 450
    LSIACYIFTA LMGVFVLKTL NMNVTN 476
    Length:476
    Mass (Da):54,072
    Last modified:November 1, 1995 - v1
    Checksum:iEFD989B50AAED348
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46719 Genomic DNA. Translation: CAA86674.1.
    U22326 Genomic DNA. Translation: AAA85158.1.
    X87611 Genomic DNA. Translation: CAA60920.1.
    Z49277 Genomic DNA. Translation: CAA89291.1.
    BK006943 Genomic DNA. Translation: DAA08789.1.
    PIRiA56510.
    RefSeqiNP_012532.3. NM_001181436.3.

    Genome annotation databases

    EnsemblFungiiYJL002C; YJL002C; YJL002C.
    GeneIDi853455.
    KEGGisce:YJL002C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46719 Genomic DNA. Translation: CAA86674.1 .
    U22326 Genomic DNA. Translation: AAA85158.1 .
    X87611 Genomic DNA. Translation: CAA60920.1 .
    Z49277 Genomic DNA. Translation: CAA89291.1 .
    BK006943 Genomic DNA. Translation: DAA08789.1 .
    PIRi A56510.
    RefSeqi NP_012532.3. NM_001181436.3.

    3D structure databases

    ProteinModelPortali P41543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33755. 81 interactions.
    DIPi DIP-2454N.
    IntActi P41543. 22 interactions.
    MINTi MINT-473095.
    STRINGi 4932.YJL002C.

    Chemistry

    BindingDBi P41543.

    Proteomic databases

    MaxQBi P41543.
    PaxDbi P41543.
    PeptideAtlasi P41543.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL002C ; YJL002C ; YJL002C .
    GeneIDi 853455.
    KEGGi sce:YJL002C.

    Organism-specific databases

    CYGDi YJL002c.
    SGDi S000003539. OST1.

    Phylogenomic databases

    eggNOGi NOG292474.
    GeneTreei ENSGT00390000009630.
    HOGENOMi HOG000093925.
    KOi K12666.
    OMAi FRIGWND.
    OrthoDBi EOG7W9S43.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:YJL002C-MONOMER.
    BRENDAi 2.4.1.119. 984.

    Miscellaneous databases

    NextBioi 974023.
    PROi P41543.

    Gene expression databases

    Genevestigatori P41543.

    Family and domain databases

    InterProi IPR007676. Ribophorin_I.
    [Graphical view ]
    PANTHERi PTHR21049. PTHR21049. 1 hit.
    Pfami PF04597. Ribophorin_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I."
      Silberstein S., Collins P.G., Kelleher D.J., Rapiejko P.J., Gilmore R.
      J. Cell Biol. 128:525-536(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-38 AND 135-165.
    2. "The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase."
      Pathak R., Parker C.S., Imperiali B.
      FEBS Lett. 362:229-234(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
      Kelleher D.J., Gilmore R.
      J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
      Karaoglu D., Kelleher D.J., Gilmore R.
      J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    7. "The oligosaccharyltransferase complex from yeast."
      Knauer R., Lehle L.
      Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
      Schwarz M., Knauer R., Lehle L.
      FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
      Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
      Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
      Yan A., Lennarz W.J.
      Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
      Chavan M., Yan A., Lennarz W.J.
      J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
    13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
      Yan A., Wu E., Lennarz W.J.
      Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.

    Entry informationi

    Entry nameiOST1_YEAST
    AccessioniPrimary (citable) accession number: P41543
    Secondary accession number(s): D6VWH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3