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Protein

General vesicular transport factor p115

Gene

Uso1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane (PubMed:7831323, PubMed:7831324, PubMed:10679020). May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (PubMed:7831323, PubMed:7831324, PubMed:10679020).3 Publications

GO - Molecular functioni

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi vesicle docking Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: RGD
  • membrane fusion Source: RGD
  • transcytosis Source: RGD
  • vesicle fusion with Golgi apparatus Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p1151 Publication
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein1 Publication
Short name:
TAP1 Publication
Vesicle-docking protein
Gene namesi
Name:Uso1
Synonyms:Vdp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi621604. Uso1.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Golgi apparatus membrane 1 Publication; Peripheral membrane protein By similarity

  • Note: Recycles between the cytosol and the Golgi apparatus during interphase.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959General vesicular transport factor p115PRO_0000065776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei202 – 2021N6-acetyllysineBy similarity
Modified residuei940 – 9401PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP41542.
PRIDEiP41542.

PTM databases

iPTMnetiP41542.
PhosphoSiteiP41542.

Expressioni

Gene expression databases

GenevisibleiP41542. RN.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (PubMed:7831323). Interacts with MIF (By similarity). Interacts with GM130/GOLGA2; interaction is disrupted upon phosphorylation of GM130/GOLGA2 by CDK1 at the onset of mitosis (PubMed:9150144, PubMed:9753325, PubMed:10744704, PubMed:10769027).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gbf1Q6DFZ13EBI-4423297,EBI-761491From a different organism.
Gosr1Q6293110EBI-4423297,EBI-7837133
Stx5Q088518EBI-4423297,EBI-2028244

Protein-protein interaction databases

IntActiP41542. 15 interactions.
MINTiMINT-4565194.
STRINGi10116.ENSRNOP00000003277.

Structurei

3D structure databases

ProteinModelPortaliP41542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati20 – 6041ARM 1Add
BLAST
Repeati61 – 12161ARM 2Add
BLAST
Repeati123 – 16341ARM 3Add
BLAST
Repeati166 – 20742ARM 4Add
BLAST
Repeati208 – 25346ARM 5Add
BLAST
Repeati255 – 31056ARM 6Add
BLAST
Repeati311 – 35444ARM 7Add
BLAST
Repeati363 – 40846ARM 8Add
BLAST
Repeati420 – 45940ARM 9Add
BLAST
Repeati473 – 51341ARM 10Add
BLAST
Repeati518 – 57154ARM 11Add
BLAST
Repeati573 – 63058ARM 12Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 637637Globular headAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili638 – 930293Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi935 – 95925Asp/Glu-rich (acidic)Add
BLAST

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.1 Publication

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41542.
OMAiYQVNQDY.
OrthoDBiEOG7K0ZBS.
PhylomeDBiP41542.
TreeFamiTF106157.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEEVEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ PENVTLLLSL LEEFDFHVRW PGVRLLTSLL
160 170 180 190 200
KQLGPPVQQI ILVSPMGVSK LMDLLADSRE IIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL KNNNSNQNFF
260 270 280 290 300
KEGSYIQRMK AWFEVGDENP GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ
410 420 430 440 450
GEIVATLLPS TIDATGNSVS AGQLLCGGLF STDSLSNWCA AVALAHALQG
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLENYT KEKLKQLIEK RIGKENYIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN
660 670 680 690 700
IVTHYKNMIR EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ
710 720 730 740 750
YNLLKVQLGK DNHHQGSHSD GAQVNGIQPE EISRLREEIE ELRSHQVLLQ
760 770 780 790 800
SQLAEKDTVI ENLRSSQVSG MSEQALATCS PRDAEQVAEL KQELSALKSQ
810 820 830 840 850
LCSQSLEITR LQTENSELQQ RAETLAKSVP VEGESELVTA AKTTDVEGRL
860 870 880 890 900
SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLYLEVT
910 920 930 940 950
DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEL

DDGDRDQDI
Length:959
Mass (Da):107,162
Last modified:November 1, 1995 - v1
Checksum:i356394B48C7E003B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti591 – 5911S → P in AAC52151 (PubMed:7831324).Curated
Sequence conflicti658 – 6581M → V in AAC52151 (PubMed:7831324).Curated
Sequence conflicti816 – 8161S → R in AAC52151 (PubMed:7831324).Curated
Sequence conflicti873 – 8731A → S in AAC52151 (PubMed:7831324).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14192 mRNA. Translation: AAA62632.1.
U15589 mRNA. Translation: AAC52151.1.
PIRiA55913.
RefSeqiNP_062252.1. NM_019379.1.
UniGeneiRn.4746.

Genome annotation databases

EnsembliENSRNOT00000003277; ENSRNOP00000003277; ENSRNOG00000002301.
GeneIDi56042.
KEGGirno:56042.
UCSCiRGD:621604. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14192 mRNA. Translation: AAA62632.1.
U15589 mRNA. Translation: AAC52151.1.
PIRiA55913.
RefSeqiNP_062252.1. NM_019379.1.
UniGeneiRn.4746.

3D structure databases

ProteinModelPortaliP41542.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP41542. 15 interactions.
MINTiMINT-4565194.
STRINGi10116.ENSRNOP00000003277.

PTM databases

iPTMnetiP41542.
PhosphoSiteiP41542.

Proteomic databases

PaxDbiP41542.
PRIDEiP41542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003277; ENSRNOP00000003277; ENSRNOG00000002301.
GeneIDi56042.
KEGGirno:56042.
UCSCiRGD:621604. rat.

Organism-specific databases

CTDi8615.
RGDi621604. Uso1.

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41542.
OMAiYQVNQDY.
OrthoDBiEOG7K0ZBS.
PhylomeDBiP41542.
TreeFamiTF106157.

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

PROiP41542.

Gene expression databases

GenevisibleiP41542. RN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p."
    Sapperstein S.K., Walter D.M., Grosvenor A.R., Heuser J.E., Waters M.G.
    Proc. Natl. Acad. Sci. U.S.A. 92:522-526(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    Tissue: Liver.
  2. "Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes."
    Barroso M., Nelson D.S., Sztul E.
    Proc. Natl. Acad. Sci. U.S.A. 92:527-531(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION.
    Tissue: Liver.
  3. "The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner."
    Nakamura N., Lowe M., Levine T.P., Rabouille C., Warren G.
    Cell 89:445-455(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOLGA2.
  4. "Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis."
    Lowe M., Rabouille C., Nakamura N., Watson R., Jackman M., Jamsa E., Rahman D., Pappin D.J.C., Warren G.
    Cell 94:783-793(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOLGA2.
  5. "Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus."
    Linstedt A.D., Jesch S.A., Mehta A., Lee T.H., Garcia-Mata R., Nelson D.S., Sztul E.
    J. Biol. Chem. 275:10196-10201(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOLGA2.
  6. "The mitotic phosphorylation cycle of the cis-Golgi matrix protein GM130."
    Lowe M., Gonatas N.K., Warren G.
    J. Cell Biol. 149:341-356(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GOLGA2.
  7. "The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo."
    Seemann J., Jokitalo E.J., Warren G.
    Mol. Biol. Cell 11:635-645(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUSO1_RAT
AccessioniPrimary (citable) accession number: P41542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.