Reviewed, UniProtKB/Swiss-Prot P41542 (USO1_RAT)

Names and origin

Protein names

Recommended name:
    General vesicular transport factor p115
Alternative name(s):
    Protein USO1 homolog
    Transcytosis-associated protein
      Short name=TAP
    Vesicle-docking protein

Gene names

Name:	Uso1
Synonyms:	Vdp

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Subunit structure	Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF By similarity.
Subcellular location	Cytoplasm › cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
Domain	Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational modification	Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation By similarity. Ref.3
Sequence similarities	Belongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Domain	Coiled coil Repeat
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	intra-Golgi vesicle-mediated transport Ref.2 Traceable author statement. Source: RGD transcytosis Ref.2 Inferred from direct assay. Source: RGD vesicle fusion with Golgi apparatus Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 959	959	General vesicular transport factor p115		PRO_0000065776
Regions
	Repeat	48 – 91	44	ARM 1
	Repeat	112 – 151	40	ARM 2
	Repeat	154 – 194	41	ARM 3
	Repeat	196 – 241	46	ARM 4
	Repeat	242 – 290	49	ARM 5
	Repeat	292 – 341	50	ARM 6
	Repeat	342 – 393	52	ARM 7
	Repeat	394 – 448	55	ARM 8
	Repeat	450 – 501	52	ARM 9
	Repeat	503 – 548	46	ARM 10
	Region	1 – 637	637	Globular head
	Coiled coil	638 – 930	293	Potential
	Compositional bias	935 – 959	25	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	940	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	591	1	S → P in AAC52151. Ref.2
	Sequence conflict	658	1	M → V in AAC52151. Ref.2
	Sequence conflict	816	1	S → R in AAC52151. Ref.2
	Sequence conflict	873	1	A → S in AAC52151. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41542-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 356394B48C7E003B	FASTA	959	107,162
	10 20 30 40 50 60 MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 70 80 90 100 110 120 AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEEVEE NSTRQSEDLG SQFTEIFIKQ 130 140 150 160 170 180 PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSK LMDLLADSRE 190 200 210 220 230 240 IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 250 260 270 280 290 300 KNNNSNQNFF KEGSYIQRMK AWFEVGDENP GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 310 320 330 340 350 360 SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 370 380 390 400 410 420 PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 430 440 450 460 470 480 AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 490 500 510 520 530 540 ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 550 560 570 580 590 600 CALLLGISIY FNDNSLENYT KEKLKQLIEK RIGKENYIEK LGFISKHELY SRASQKPQPN 610 620 630 640 650 660 FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 670 680 690 700 710 720 EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ YNLLKVQLGK DNHHQGSHSD 730 740 750 760 770 780 GAQVNGIQPE EISRLREEIE ELRSHQVLLQ SQLAEKDTVI ENLRSSQVSG MSEQALATCS 790 800 810 820 830 840 PRDAEQVAEL KQELSALKSQ LCSQSLEITR LQTENSELQQ RAETLAKSVP VEGESELVTA 850 860 870 880 890 900 AKTTDVEGRL SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLYLEVT 910 920 930 940 950 DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEL DDGDRDQDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p." Sapperstein S.K., Walter D.M., Grosvenor A.R., Heuser J.E., Waters M.G. Proc. Natl. Acad. Sci. U.S.A. 92:522-526(1995) [PubMed: 7831323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes." Barroso M., Nelson D.S., Sztul E. Proc. Natl. Acad. Sci. U.S.A. 92:527-531(1995) [PubMed: 7831324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[3]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, MASS SPECTROMETRY. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14192 mRNA. Translation: AAA62632.1. U15589 mRNA. Translation: AAC52151.1.
IPI	IPI00324618.
PIR	A55913.
RefSeq	NP_062252.1.
UniGene	Rn.4746
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P41542.
PTM databases
PhosphoSite	P41542.
Proteomic databases
PRIDE	P41542.
Genome annotation databases
Ensembl	ENSRNOT00000003277; ENSRNOP00000003277; ENSRNOG00000002301; Rattus norvegicus. [Genome view]
GeneID	56042.
KEGG	rno:56042.
UCSC	NM_019379. rat.
Organism-specific databases
CTD	56042.
RGD	621604. Uso1.
Phylogenomic databases
eggNOG	roNOG06411.
HOVERGEN	P41542.
InParanoid	P41542.
PhylomeDB	P41542.
Gene expression databases
ArrayExpress	P41542.
Genevestigator	P41542.
GermOnline	ENSRNOG00000002301. Rattus norvegicus.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR006955. Uso1_p115_C. IPR006953. Uso1_p115_head. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF04871. Uso1_p115_C. 1 hit. PF04869. Uso1_p115_head. 1 hit. [Graphical view]
PROSITE	PS50176. ARM_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611086.

Entry information

Entry name

USO1_RAT

Accession

Primary (citable) accession number: P41542

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)