General vesicular transport factor p115

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P41542 (USO1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
General vesicular transport factor p115

Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name=TAP
Vesicle-docking protein

Gene names

Name:	Uso1
Synonyms:	Vdp

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Subunit structure	Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF By similarity.
Subcellular location	Cytoplasm › cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase.
Domain	Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational modification	Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promostes dissociation By similarity. Ref.3
Sequence similarities	Belongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Domain	Coiled coil Repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	intra-Golgi vesicle-mediated transport Traceable author statement Ref.2. Source: RGD transcytosis Inferred from direct assay Ref.2. Source: RGD vesicle fusion with Golgi apparatus Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 959

959

General vesicular transport factor p115

PRO_0000065776

Regions

Repeat

48 – 91

ARM 1

Repeat

112 – 151

ARM 2

Repeat

154 – 194

ARM 3

Repeat

196 – 241

ARM 4

Repeat

242 – 290

ARM 5

Repeat

292 – 341

ARM 6

Repeat

342 – 393

ARM 7

Repeat

394 – 448

ARM 8

Repeat

450 – 501

ARM 9

Repeat

503 – 548

ARM 10

Region

1 – 637

637

Globular head

Coiled coil

638 – 930

293

Potential

Compositional bias

935 – 959

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

202

N6-acetyllysine By similarity

Modified residue

940

Phosphoserine Ref.3

Experimental info

Sequence conflict

591

S → P in AAC52151. Ref.2

Sequence conflict

658

M → V in AAC52151. Ref.2

Sequence conflict

816

S → R in AAC52151. Ref.2

Sequence conflict

873

A → S in AAC52151. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P41542 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 356394B48C7E003B
Show »

FASTA

959

107,162

        10         20         30         40         50         60 
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 

        70         80         90        100        110        120 
AMEHLIHVLQ TDRSDSEIIA YALDTLYNII SNDEEEEVEE NSTRQSEDLG SQFTEIFIKQ 

       130        140        150        160        170        180 
PENVTLLLSL LEEFDFHVRW PGVRLLTSLL KQLGPPVQQI ILVSPMGVSK LMDLLADSRE 

       190        200        210        220        230        240 
IIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL 

       250        260        270        280        290        300 
KNNNSNQNFF KEGSYIQRMK AWFEVGDENP GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 

       310        320        330        340        350        360 
SSCQKAMFQC GLLQQLCTIL MATGIPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 

       370        380        390        400        410        420 
PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNEKGQ GEIVATLLPS TIDATGNSVS 

       430        440        450        460        470        480 
AGQLLCGGLF STDSLSNWCA AVALAHALQG NATQKEQLLR VQLATSIGNP PVSLLQQCTN 

       490        500        510        520        530        540 
ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 

       550        560        570        580        590        600 
CALLLGISIY FNDNSLENYT KEKLKQLIEK RIGKENYIEK LGFISKHELY SRASQKPQPN 

       610        620        630        640        650        660 
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 

       670        680        690        700        710        720 
EQDLQLEELK QQVSTLKCQN EQLQTAVTQQ ASQIQQHKDQ YNLLKVQLGK DNHHQGSHSD 

       730        740        750        760        770        780 
GAQVNGIQPE EISRLREEIE ELRSHQVLLQ SQLAEKDTVI ENLRSSQVSG MSEQALATCS 

       790        800        810        820        830        840 
PRDAEQVAEL KQELSALKSQ LCSQSLEITR LQTENSELQQ RAETLAKSVP VEGESELVTA 

       850        860        870        880        890        900 
AKTTDVEGRL SALLQETKEL KNEIKALSEE RTAIQKQLDS SNSTIAILQT EKDKLYLEVT 

       910        920        930        940        950 
DSKKEQDDLL VLLADQDQKI LSLKSKLKDL GHPVEEEDES GDQEDDDDEL DDGDRDQDI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p." Sapperstein S.K., Walter D.M., Grosvenor A.R., Heuser J.E., Waters M.G. Proc. Natl. Acad. Sci. U.S.A. 92:522-526(1995) [PubMed: 7831323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes." Barroso M., Nelson D.S., Sztul E. Proc. Natl. Acad. Sci. U.S.A. 92:527-531(1995) [PubMed: 7831324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[3]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940, MASS SPECTROMETRY. Tissue: Renal collecting duct.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14192 mRNA. Translation: AAA62632.1. U15589 mRNA. Translation: AAC52151.1.
IPI	IPI00324618.
PIR	A55913.
RefSeq	NP_062252.1. NM_019379.1.
UniGene	Rn.4746.
3D structure databases
ProteinModelPortal	P41542.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41542. 2 interactions.
STRING	P41542.
PTM databases
PhosphoSite	P41542.
Proteomic databases
PRIDE	P41542.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003277; ENSRNOP00000003277; ENSRNOG00000002301.
GeneID	56042.
KEGG	rno:56042.
UCSC	NM_019379. rat.
Organism-specific databases
CTD	8615.
RGD	621604. Uso1.
Phylogenomic databases
eggNOG	roNOG06411.
GeneTree	ENSGT00390000017018.
HOVERGEN	HBG018067.
InParanoid	P41542.
OrthoDB	EOG4PC9RK.
PhylomeDB	P41542.
Gene expression databases
ArrayExpress	P41542.
Genevestigator	P41542.
GermOnline	ENSRNOG00000002301. Rattus norvegicus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR006955. Uso1_p115_C. IPR024095. Vesicle_P115-like. IPR006953. Vesicle_Uso1_P115_head. [Graphical view]
PANTHER	PTHR10013. PTHR10013. 1 hit.
Pfam	PF04871. Uso1_p115_C. 1 hit. PF04869. Uso1_p115_head. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 2 hits.
PROSITE	PS50176. ARM_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611086.

Entry information

Entry name

USO1_RAT

Accession

Primary (citable) accession number: P41542

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents