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Protein

General vesicular transport factor p115

Gene

USO1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

  • identical protein binding Source: AgBase
  • poly(A) RNA binding Source: Ensembl
  • protein transporter activity Source: AgBase

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-BTA-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:USO1
Synonyms:VDP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961General vesicular transport factor p115PRO_0000065773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei202 – 2021N6-acetyllysineBy similarity
Modified residuei941 – 9411PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP41541.
PeptideAtlasiP41541.
PRIDEiP41541.

PTM databases

iPTMnetiP41541.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017028.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: AgBase

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022642.

Structurei

Secondary structure

1
961
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 3214Combined sources
Helixi36 – 4813Combined sources
Turni49 – 557Combined sources
Helixi56 – 605Combined sources
Helixi62 – 709Combined sources
Helixi76 – 9015Combined sources
Beta strandi93 – 953Combined sources
Helixi109 – 11810Combined sources
Helixi122 – 1309Combined sources
Helixi136 – 15217Combined sources
Helixi154 – 16310Combined sources
Helixi167 – 1726Combined sources
Helixi173 – 1764Combined sources
Helixi180 – 19415Combined sources
Helixi198 – 2069Combined sources
Helixi209 – 22012Combined sources
Helixi222 – 2243Combined sources
Helixi226 – 24015Combined sources
Helixi244 – 2529Combined sources
Helixi256 – 2638Combined sources
Helixi274 – 29017Combined sources
Helixi297 – 30913Combined sources
Helixi312 – 3209Combined sources
Helixi327 – 34115Combined sources
Helixi345 – 3539Combined sources
Beta strandi355 – 3606Combined sources
Helixi364 – 3729Combined sources
Helixi379 – 39315Combined sources
Helixi397 – 4059Combined sources
Beta strandi417 – 4193Combined sources
Helixi421 – 4299Combined sources
Helixi434 – 44815Combined sources
Helixi452 – 4587Combined sources
Helixi474 – 4818Combined sources
Turni482 – 4854Combined sources
Helixi488 – 50215Combined sources
Helixi506 – 5149Combined sources
Helixi518 – 52710Combined sources
Helixi532 – 55019Combined sources
Helixi557 – 5593Combined sources
Helixi561 – 57111Combined sources
Helixi574 – 58613Combined sources
Helixi590 – 5956Combined sources
Helixi604 – 6063Combined sources
Helixi611 – 62919Combined sources
Helixi633 – 6386Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GQ2X-ray2.18A/B1-651[»]
3GRLX-ray2.00A1-651[»]
ProteinModelPortaliP41541.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41541.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati20 – 6041ARM 1PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati61 – 12161ARM 2PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati123 – 16341ARM 3PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati166 – 20742ARM 4PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati208 – 25346ARM 5PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati255 – 31056ARM 6PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati311 – 35444ARM 7PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati363 – 40846ARM 8PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati420 – 45940ARM 9PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati473 – 51341ARM 10PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati518 – 57154ARM 11PROSITE-ProRule annotation1 PublicationAdd
BLAST
Repeati573 – 63058ARM 12PROSITE-ProRule annotation1 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 641641Globular headAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili642 – 929288Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi934 – 96128Asp/Glu-rich (acidic)Add
BLAST

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.1 Publication

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41541.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
TreeFamiTF106157.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNDEEEEVEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ QENVTLLLSL LEEFDFHVRW PGVKLLTSLL
160 170 180 190 200
KQLGPQVQQI ILVSPMGVSR LMDLLADSRE VIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL KNNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGDENS GWSAQKVTNL HLMLQLVRVL VSPNNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGVPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNQKGQ
410 420 430 440 450
GEIVSTLLPS TIDATGNTVS AGQLLCGGLF STDSLSNWCA AVALAHALQE
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLETYM KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDS
660 670 680 690 700
IVTHYKNMIR EQDLQLEELK QQISTLKCQN EQLQTAVTQQ VSQIQQHKDQ
710 720 730 740 750
YNLLKVQLGK DSQHQGPYTD GAQMNGVQPE EISRLREEIE ELKSNRELLQ
760 770 780 790 800
SQLAEKDSLI ENLKSSQLSP GTNEQSSATA GDSEQIAELK QELATLKSQL
810 820 830 840 850
NSQSVEITKL QTEKQELLQK TEAFAKSAPV PGESETVIAT KTTDVEGRLS
860 870 880 890 900
ALLQETKELK NEIKALSEER TAIKEQLDSS NSTIAILQNE KNKLEVDITD
910 920 930 940 950
SKKEQDDLLV LLADQDQKIF SLKNKLKELG HPVEEEDELE SGDQDDEDDE
960
DEDDGKEQGH I
Length:961
Mass (Da):107,515
Last modified:November 1, 1995 - v1
Checksum:i993FEB7F90ABC0AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti335 – 3351N → K in AAI08113 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14186 mRNA. Translation: AAA62631.1.
BC108112 mRNA. Translation: AAI08113.1.
RefSeqiNP_777270.2. NM_174845.2.
UniGeneiBt.5308.

Genome annotation databases

EnsembliENSBTAT00000022642; ENSBTAP00000022642; ENSBTAG00000017028.
GeneIDi317724.
KEGGibta:317724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14186 mRNA. Translation: AAA62631.1.
BC108112 mRNA. Translation: AAI08113.1.
RefSeqiNP_777270.2. NM_174845.2.
UniGeneiBt.5308.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GQ2X-ray2.18A/B1-651[»]
3GRLX-ray2.00A1-651[»]
ProteinModelPortaliP41541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022642.

PTM databases

iPTMnetiP41541.

Proteomic databases

PaxDbiP41541.
PeptideAtlasiP41541.
PRIDEiP41541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022642; ENSBTAP00000022642; ENSBTAG00000017028.
GeneIDi317724.
KEGGibta:317724.

Organism-specific databases

CTDi8615.

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41541.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
TreeFamiTF106157.

Enzyme and pathway databases

ReactomeiR-BTA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-BTA-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

EvolutionaryTraceiP41541.

Gene expression databases

BgeeiENSBTAG00000017028.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSO1_BOVIN
AccessioniPrimary (citable) accession number: P41541
Secondary accession number(s): Q32PH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.