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Protein

General vesicular transport factor p115

Gene

USO1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

  • identical protein binding Source: AgBase
  • poly(A) RNA binding Source: Ensembl
  • protein transporter activity Source: AgBase

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-BTA-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:USO1
Synonyms:VDP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657731 – 961General vesicular transport factor p115Add BLAST961

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineBy similarity1
Modified residuei202N6-acetyllysineBy similarity1
Modified residuei941PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP41541.
PeptideAtlasiP41541.
PRIDEiP41541.

PTM databases

iPTMnetiP41541.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017028.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure. Interacts with MIF (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: AgBase

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022642.

Structurei

Secondary structure

1961
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 32Combined sources14
Helixi36 – 48Combined sources13
Turni49 – 55Combined sources7
Helixi56 – 60Combined sources5
Helixi62 – 70Combined sources9
Helixi76 – 90Combined sources15
Beta strandi93 – 95Combined sources3
Helixi109 – 118Combined sources10
Helixi122 – 130Combined sources9
Helixi136 – 152Combined sources17
Helixi154 – 163Combined sources10
Helixi167 – 172Combined sources6
Helixi173 – 176Combined sources4
Helixi180 – 194Combined sources15
Helixi198 – 206Combined sources9
Helixi209 – 220Combined sources12
Helixi222 – 224Combined sources3
Helixi226 – 240Combined sources15
Helixi244 – 252Combined sources9
Helixi256 – 263Combined sources8
Helixi274 – 290Combined sources17
Helixi297 – 309Combined sources13
Helixi312 – 320Combined sources9
Helixi327 – 341Combined sources15
Helixi345 – 353Combined sources9
Beta strandi355 – 360Combined sources6
Helixi364 – 372Combined sources9
Helixi379 – 393Combined sources15
Helixi397 – 405Combined sources9
Beta strandi417 – 419Combined sources3
Helixi421 – 429Combined sources9
Helixi434 – 448Combined sources15
Helixi452 – 458Combined sources7
Helixi474 – 481Combined sources8
Turni482 – 485Combined sources4
Helixi488 – 502Combined sources15
Helixi506 – 514Combined sources9
Helixi518 – 527Combined sources10
Helixi532 – 550Combined sources19
Helixi557 – 559Combined sources3
Helixi561 – 571Combined sources11
Helixi574 – 586Combined sources13
Helixi590 – 595Combined sources6
Helixi604 – 606Combined sources3
Helixi611 – 629Combined sources19
Helixi633 – 638Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GQ2X-ray2.18A/B1-651[»]
3GRLX-ray2.00A1-651[»]
ProteinModelPortaliP41541.
SMRiP41541.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41541.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 60ARM 1PROSITE-ProRule annotation1 PublicationAdd BLAST41
Repeati61 – 121ARM 2PROSITE-ProRule annotation1 PublicationAdd BLAST61
Repeati123 – 163ARM 3PROSITE-ProRule annotation1 PublicationAdd BLAST41
Repeati166 – 207ARM 4PROSITE-ProRule annotation1 PublicationAdd BLAST42
Repeati208 – 253ARM 5PROSITE-ProRule annotation1 PublicationAdd BLAST46
Repeati255 – 310ARM 6PROSITE-ProRule annotation1 PublicationAdd BLAST56
Repeati311 – 354ARM 7PROSITE-ProRule annotation1 PublicationAdd BLAST44
Repeati363 – 408ARM 8PROSITE-ProRule annotation1 PublicationAdd BLAST46
Repeati420 – 459ARM 9PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati473 – 513ARM 10PROSITE-ProRule annotation1 PublicationAdd BLAST41
Repeati518 – 571ARM 11PROSITE-ProRule annotation1 PublicationAdd BLAST54
Repeati573 – 630ARM 12PROSITE-ProRule annotation1 PublicationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 641Globular headAdd BLAST641

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili642 – 929Sequence analysisAdd BLAST288

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi934 – 961Asp/Glu-rich (acidic)Add BLAST28

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.1 Publication

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41541.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
TreeFamiTF106157.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNDEEEEVEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ QENVTLLLSL LEEFDFHVRW PGVKLLTSLL
160 170 180 190 200
KQLGPQVQQI ILVSPMGVSR LMDLLADSRE VIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIITEE GNSDGGIVVE DCLILLQNLL KNNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGDENS GWSAQKVTNL HLMLQLVRVL VSPNNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGVPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNQKGQ
410 420 430 440 450
GEIVSTLLPS TIDATGNTVS AGQLLCGGLF STDSLSNWCA AVALAHALQE
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLETYM KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDS
660 670 680 690 700
IVTHYKNMIR EQDLQLEELK QQISTLKCQN EQLQTAVTQQ VSQIQQHKDQ
710 720 730 740 750
YNLLKVQLGK DSQHQGPYTD GAQMNGVQPE EISRLREEIE ELKSNRELLQ
760 770 780 790 800
SQLAEKDSLI ENLKSSQLSP GTNEQSSATA GDSEQIAELK QELATLKSQL
810 820 830 840 850
NSQSVEITKL QTEKQELLQK TEAFAKSAPV PGESETVIAT KTTDVEGRLS
860 870 880 890 900
ALLQETKELK NEIKALSEER TAIKEQLDSS NSTIAILQNE KNKLEVDITD
910 920 930 940 950
SKKEQDDLLV LLADQDQKIF SLKNKLKELG HPVEEEDELE SGDQDDEDDE
960
DEDDGKEQGH I
Length:961
Mass (Da):107,515
Last modified:November 1, 1995 - v1
Checksum:i993FEB7F90ABC0AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335N → K in AAI08113 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14186 mRNA. Translation: AAA62631.1.
BC108112 mRNA. Translation: AAI08113.1.
RefSeqiNP_777270.2. NM_174845.2.
UniGeneiBt.5308.

Genome annotation databases

EnsembliENSBTAT00000022642; ENSBTAP00000022642; ENSBTAG00000017028.
GeneIDi317724.
KEGGibta:317724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14186 mRNA. Translation: AAA62631.1.
BC108112 mRNA. Translation: AAI08113.1.
RefSeqiNP_777270.2. NM_174845.2.
UniGeneiBt.5308.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GQ2X-ray2.18A/B1-651[»]
3GRLX-ray2.00A1-651[»]
ProteinModelPortaliP41541.
SMRiP41541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022642.

PTM databases

iPTMnetiP41541.

Proteomic databases

PaxDbiP41541.
PeptideAtlasiP41541.
PRIDEiP41541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022642; ENSBTAP00000022642; ENSBTAG00000017028.
GeneIDi317724.
KEGGibta:317724.

Organism-specific databases

CTDi8615.

Phylogenomic databases

eggNOGiKOG0946. Eukaryota.
ENOG410XRCG. LUCA.
GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiP41541.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
TreeFamiTF106157.

Enzyme and pathway databases

ReactomeiR-BTA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-BTA-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

EvolutionaryTraceiP41541.

Gene expression databases

BgeeiENSBTAG00000017028.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSO1_BOVIN
AccessioniPrimary (citable) accession number: P41541
Secondary accession number(s): Q32PH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.