P41513 (GYRA_ERWCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Erwinia carotovora

Taxonomic identifier

554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145233
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41513 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6F7D181BADA617E3	FASTA	878	97,589
	10 20 30 40 50 60 MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMSVLGNDWN 70 80 90 100 110 120 KPYKKSARVV GDVIGKYHPH GDSAVYETIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 130 140 150 160 170 180 RYTEIRMSKI AHELLADLEK ETVDFVPNYD GTEQIPDVMP TRIPNLLVNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLTEV VNGCLAYIDD ENISLEGLME HIKGPDFPTA AIINGRRGIE EAYRTGRGKI 250 260 270 280 290 300 YIRARAEVEA DAKTGRETII VHEIPYQVNK ARLIEKIAEL VKDKRIEGIS ALRDESDKDG 310 320 330 340 350 360 MRIVIEIKRD AVGEVVLNNL YSQTQLQTSF GINMVALHQG QPKIMPLKDI LVAFVRHRRE 370 380 390 400 410 420 VVTRRTIFEL RKARDRAHIL EGLAIALANI DPIIELIRRA ASPAEAKASL IAQAWELGSV 430 440 450 460 470 480 ATMLERAGDD AARPEWLEPE FGIRDGRYYL TEQQAQAILD LRLQKLTGME HEKLLDEYKE 490 500 510 520 530 540 LLAEIAELLY ILNSPERLME VIREELEAFK TQYSDERRTE ITANTADINI EDLINQEDVV 550 560 570 580 590 600 VTLSHQGYVK YQPLSDYEAQ RRGGKGKSAA RIKEEDFIDR LLVANTHDTI LCFSSRGRLY 610 620 630 640 650 660 WMKVYQLPEA SRGARGRPIV NLLPLEADER ITAILPVREY EEGRHIFMAT ASGTVKKTAL 670 680 690 700 710 720 TEFSRHVSGI IAVNLNEGDE LIGVDLTDGS DEAMLFSAEG KVVRFSEQAV RSMGRTATGV 730 740 750 760 770 780 RGINLQGEDR VVSLIIPRGE GDILTVTQNG FGKRTAVSEY PTKSRATKGV ISIKVSERNG 790 800 810 820 830 840 KVVGAVQVDA ADQIMMITDA GTLVRTRVSE VSIVGRNTQG VTLIRTAEDE HVVGLQRVAE 850 860 870 PVEDEELDGV VKVEGEVAED DDAIDDIDGD DDIAEDDE

References

[1]	"Cloning and sequencing of the gyrA gene from the plant pathogen Erwinia carotovora." Rosanas A., Barbe J., Gibert I. Gene 161:11-14(1995) [PubMed: 7642123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80798 Genomic DNA. Translation: CAA56777.1.
PIR	S47332.
3D structure databases
ProteinModelPortal	P41513.
SMR	P41513. Positions 30-522, 536-840.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_ERWCA

Accession

Primary (citable) accession number: P41513

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program