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P41512

- TOP1_XENLA

UniProt

P41512 - TOP1_XENLA

Protein

DNA topoisomerase 1

Gene

top1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei372 – 3721Interaction with DNABy similarity
    Sitei420 – 4201Interaction with DNABy similarity
    Sitei468 – 4681Interaction with DNABy similarity
    Sitei499 – 4991Interaction with DNABy similarity
    Sitei557 – 5571Interaction with DNABy similarity
    Sitei588 – 5881Interaction with DNABy similarity
    Sitei630 – 6301Interaction with DNABy similarity
    Sitei688 – 6881Interaction with DNABy similarity
    Sitei706 – 7061Interaction with DNABy similarity
    Active sitei779 – 7791O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Gene namesi
    Name:top1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-950006. top1.1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 829829DNA topoisomerase 1PRO_0000145204Add
    BLAST

    Proteomic databases

    PRIDEiP41512.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP41512.
    SMRiP41512. Positions 255-808.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni481 – 4822Interaction with DNABy similarity
    Regioni544 – 5496Interaction with DNABy similarity
    Regioni641 – 6433Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Phylogenomic databases

    HOVERGENiHBG007988.
    KOiK03163.

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41512-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEDHVQNDS QIEAVFRVND SHKHKKDKEH RHKEHKKDKD REKSKHNNSE    50
    HRDPSEKKHK DKHKNNDKHR EKDGEKHRER DGEKHRDKNG EKHRDGEKHK 100
    EKDIEKHKEV EKHRVKDGEK HKEKDVEKHK EKDVEKHRDG EKHKHRDKDR 150
    EKKKEEKMKS SSGGVKVKKE NGFSSPVRVK DEPEDQGFYV SPKENKAMKR 200
    PREDDEDYKP KKIKSEDDKK GKKRKQEEED IKPKKKSKAK GNEEGVKKKK 250
    VKKEEEEKWK WWEEERHRDG IKWKFLEHKG PVFAPPYEPV PDNVKFYYDG 300
    NLVKLSPKAE EVATFFAKML DHEYTTKDIF RKNFFKDWKK EMTTDERNLI 350
    TNLSKCDFNA MSLYFKEQSE ARKNMTKEEK LKIKAENERL LQEYGYCIMD 400
    NHKERIANFR IEPPGLFRGR GDHPKMGKLK KRIMPEDIII NCSKDSKIPV 450
    APAGHKWKEV RHDGKVTWLV SWTENIQGSI KYIMLNPSSR IKGEKDWQKY 500
    ETARRLKMCV EKIRNTYKED WKSKEMKVRQ RAVALYFIDK LALRAGNEKE 550
    EGETADTVGC CSLRVEHINL FQELDGQEFV VEFDFPGKDS IRYYNKVPVE 600
    KRVFKNLQLF MENKQPDDDL FDRLNTSILN KHLQDLMEGL TAKVFRTYNA 650
    SITLQQQLDE LTNSDDNVPA KILSYNRANR AVAILCNHQR APPKTFEKSM 700
    MNLQGKIDAK KDQLADARRE FKSAKADAKV RRDEKTKKLV ESKKKAVQRI 750
    EEQLMKLEVQ ATDREENKQI ALGTSKLNYL DPRISVAWCK KYGVPIEKIY 800
    NKTQRKNLLG PSIWQTTTSN FNAEQRCFS 829
    Length:829
    Mass (Da):98,231
    Last modified:November 1, 1995 - v1
    Checksum:i8D1FE4252A916219
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07777 mRNA. Translation: AAB36608.1.
    PIRiS72366.
    RefSeqiNP_001084031.1. NM_001090562.1.
    UniGeneiXl.62.

    Genome annotation databases

    GeneIDi399263.
    KEGGixla:399263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07777 mRNA. Translation: AAB36608.1 .
    PIRi S72366.
    RefSeqi NP_001084031.1. NM_001090562.1.
    UniGenei Xl.62.

    3D structure databases

    ProteinModelPortali P41512.
    SMRi P41512. Positions 255-808.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P41512.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399263.
    KEGGi xla:399263.

    Organism-specific databases

    CTDi 399263.
    Xenbasei XB-GENE-950006. top1.1.

    Phylogenomic databases

    HOVERGENi HBG007988.
    KOi K03163.

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the gene for the somatic form of DNA topoisomerase I from Xenopus laevis."
      Pandit S.D., Richard R.E., Sternglanz R., Bogenhagen D.F.
      Nucleic Acids Res. 24:3593-3600(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiTOP1_XENLA
    AccessioniPrimary (citable) accession number: P41512
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3