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P41512

- TOP1_XENLA

UniProt

P41512 - TOP1_XENLA

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Protein
DNA topoisomerase 1
Gene
top1
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei372 – 3721Interaction with DNA By similarity
Sitei420 – 4201Interaction with DNA By similarity
Sitei468 – 4681Interaction with DNA By similarity
Sitei499 – 4991Interaction with DNA By similarity
Sitei557 – 5571Interaction with DNA By similarity
Sitei588 – 5881Interaction with DNA By similarity
Sitei630 – 6301Interaction with DNA By similarity
Sitei688 – 6881Interaction with DNA By similarity
Sitei706 – 7061Interaction with DNA By similarity
Active sitei779 – 7791O-(3'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:top1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-950006. top1.1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829DNA topoisomerase 1
PRO_0000145204Add
BLAST

Proteomic databases

PRIDEiP41512.

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP41512.
SMRiP41512. Positions 255-808.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni481 – 4822Interaction with DNA By similarity
Regioni544 – 5496Interaction with DNA By similarity
Regioni641 – 6433Interaction with DNA By similarity

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG007988.
KOiK03163.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41512-1 [UniParc]FASTAAdd to Basket

« Hide

MSEDHVQNDS QIEAVFRVND SHKHKKDKEH RHKEHKKDKD REKSKHNNSE    50
HRDPSEKKHK DKHKNNDKHR EKDGEKHRER DGEKHRDKNG EKHRDGEKHK 100
EKDIEKHKEV EKHRVKDGEK HKEKDVEKHK EKDVEKHRDG EKHKHRDKDR 150
EKKKEEKMKS SSGGVKVKKE NGFSSPVRVK DEPEDQGFYV SPKENKAMKR 200
PREDDEDYKP KKIKSEDDKK GKKRKQEEED IKPKKKSKAK GNEEGVKKKK 250
VKKEEEEKWK WWEEERHRDG IKWKFLEHKG PVFAPPYEPV PDNVKFYYDG 300
NLVKLSPKAE EVATFFAKML DHEYTTKDIF RKNFFKDWKK EMTTDERNLI 350
TNLSKCDFNA MSLYFKEQSE ARKNMTKEEK LKIKAENERL LQEYGYCIMD 400
NHKERIANFR IEPPGLFRGR GDHPKMGKLK KRIMPEDIII NCSKDSKIPV 450
APAGHKWKEV RHDGKVTWLV SWTENIQGSI KYIMLNPSSR IKGEKDWQKY 500
ETARRLKMCV EKIRNTYKED WKSKEMKVRQ RAVALYFIDK LALRAGNEKE 550
EGETADTVGC CSLRVEHINL FQELDGQEFV VEFDFPGKDS IRYYNKVPVE 600
KRVFKNLQLF MENKQPDDDL FDRLNTSILN KHLQDLMEGL TAKVFRTYNA 650
SITLQQQLDE LTNSDDNVPA KILSYNRANR AVAILCNHQR APPKTFEKSM 700
MNLQGKIDAK KDQLADARRE FKSAKADAKV RRDEKTKKLV ESKKKAVQRI 750
EEQLMKLEVQ ATDREENKQI ALGTSKLNYL DPRISVAWCK KYGVPIEKIY 800
NKTQRKNLLG PSIWQTTTSN FNAEQRCFS 829
Length:829
Mass (Da):98,231
Last modified:November 1, 1995 - v1
Checksum:i8D1FE4252A916219
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07777 mRNA. Translation: AAB36608.1.
PIRiS72366.
RefSeqiNP_001084031.1. NM_001090562.1.
UniGeneiXl.62.

Genome annotation databases

GeneIDi399263.
KEGGixla:399263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07777 mRNA. Translation: AAB36608.1 .
PIRi S72366.
RefSeqi NP_001084031.1. NM_001090562.1.
UniGenei Xl.62.

3D structure databases

ProteinModelPortali P41512.
SMRi P41512. Positions 255-808.
ModBasei Search...

Proteomic databases

PRIDEi P41512.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 399263.
KEGGi xla:399263.

Organism-specific databases

CTDi 399263.
Xenbasei XB-GENE-950006. top1.1.

Phylogenomic databases

HOVERGENi HBG007988.
KOi K03163.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the gene for the somatic form of DNA topoisomerase I from Xenopus laevis."
    Pandit S.D., Richard R.E., Sternglanz R., Bogenhagen D.F.
    Nucleic Acids Res. 24:3593-3600(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTOP1_XENLA
AccessioniPrimary (citable) accession number: P41512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3
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