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P41512 (TOP1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:top1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Subcellular location

Nucleus By similarity.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829DNA topoisomerase 1
PRO_0000145204

Regions

Region481 – 4822Interaction with DNA By similarity
Region544 – 5496Interaction with DNA By similarity
Region641 – 6433Interaction with DNA By similarity

Sites

Active site7791O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site3721Interaction with DNA By similarity
Site4201Interaction with DNA By similarity
Site4681Interaction with DNA By similarity
Site4991Interaction with DNA By similarity
Site5571Interaction with DNA By similarity
Site5881Interaction with DNA By similarity
Site6301Interaction with DNA By similarity
Site6881Interaction with DNA By similarity
Site7061Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P41512 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8D1FE4252A916219

FASTA82998,231
        10         20         30         40         50         60 
MSEDHVQNDS QIEAVFRVND SHKHKKDKEH RHKEHKKDKD REKSKHNNSE HRDPSEKKHK 

        70         80         90        100        110        120 
DKHKNNDKHR EKDGEKHRER DGEKHRDKNG EKHRDGEKHK EKDIEKHKEV EKHRVKDGEK 

       130        140        150        160        170        180 
HKEKDVEKHK EKDVEKHRDG EKHKHRDKDR EKKKEEKMKS SSGGVKVKKE NGFSSPVRVK 

       190        200        210        220        230        240 
DEPEDQGFYV SPKENKAMKR PREDDEDYKP KKIKSEDDKK GKKRKQEEED IKPKKKSKAK 

       250        260        270        280        290        300 
GNEEGVKKKK VKKEEEEKWK WWEEERHRDG IKWKFLEHKG PVFAPPYEPV PDNVKFYYDG 

       310        320        330        340        350        360 
NLVKLSPKAE EVATFFAKML DHEYTTKDIF RKNFFKDWKK EMTTDERNLI TNLSKCDFNA 

       370        380        390        400        410        420 
MSLYFKEQSE ARKNMTKEEK LKIKAENERL LQEYGYCIMD NHKERIANFR IEPPGLFRGR 

       430        440        450        460        470        480 
GDHPKMGKLK KRIMPEDIII NCSKDSKIPV APAGHKWKEV RHDGKVTWLV SWTENIQGSI 

       490        500        510        520        530        540 
KYIMLNPSSR IKGEKDWQKY ETARRLKMCV EKIRNTYKED WKSKEMKVRQ RAVALYFIDK 

       550        560        570        580        590        600 
LALRAGNEKE EGETADTVGC CSLRVEHINL FQELDGQEFV VEFDFPGKDS IRYYNKVPVE 

       610        620        630        640        650        660 
KRVFKNLQLF MENKQPDDDL FDRLNTSILN KHLQDLMEGL TAKVFRTYNA SITLQQQLDE 

       670        680        690        700        710        720 
LTNSDDNVPA KILSYNRANR AVAILCNHQR APPKTFEKSM MNLQGKIDAK KDQLADARRE 

       730        740        750        760        770        780 
FKSAKADAKV RRDEKTKKLV ESKKKAVQRI EEQLMKLEVQ ATDREENKQI ALGTSKLNYL 

       790        800        810        820 
DPRISVAWCK KYGVPIEKIY NKTQRKNLLG PSIWQTTTSN FNAEQRCFS 

« Hide

References

[1]"Cloning and characterization of the gene for the somatic form of DNA topoisomerase I from Xenopus laevis."
Pandit S.D., Richard R.E., Sternglanz R., Bogenhagen D.F.
Nucleic Acids Res. 24:3593-3600(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07777 mRNA. Translation: AAB36608.1.
PIRS72366.
RefSeqNP_001084031.1. NM_001090562.1.
UniGeneXl.62.

3D structure databases

ProteinModelPortalP41512.
SMRP41512. Positions 255-808.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP41512.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399263.
KEGGxla:399263.

Organism-specific databases

CTD399263.
XenbaseXB-GENE-950006. top1.1.

Phylogenomic databases

HOVERGENHBG007988.
KOK03163.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_XENLA
AccessionPrimary (citable) accession number: P41512
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families