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P41511 (TOP1_USTMA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:TOP1
ORF Names:UM05101
OrganismUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) [Reference proteome]
Taxonomic identifier237631 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

Protein attributes

Sequence length1019 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10191019DNA topoisomerase 1
PRO_0000145210

Regions

Region379 – 3802Interaction with DNA By similarity
Region442 – 4476Interaction with DNA By similarity
Region556 – 5583Interaction with DNA By similarity
Compositional bias108 – 11811Poly-Asp
Compositional bias121 – 1244Poly-Asp
Compositional bias127 – 13610Poly-Asp
Compositional bias856 – 8594Poly-Glu

Sites

Active site8221O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site3171Interaction with DNA By similarity
Site3661Interaction with DNA By similarity
Site3971Interaction with DNA By similarity
Site4541Interaction with DNA By similarity
Site4801Interaction with DNA By similarity
Site5451Interaction with DNA By similarity
Site6131Interaction with DNA By similarity
Site6321Interaction with DNA By similarity

Experimental info

Sequence conflict1811P → A in AAC37415. Ref.1
Sequence conflict1971W → C in AAC37415. Ref.1
Sequence conflict3411A → G in AAC37415. Ref.1
Sequence conflict4341W → C in AAC37415. Ref.1
Sequence conflict4521A → T in AAC37415. Ref.1
Sequence conflict10111E → G in AAC37415. Ref.1
Sequence conflict10171A → S in AAC37415. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41511 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 7E89EDE7201E7FA2

FASTA1,019115,545
        10         20         30         40         50         60 
MNSIQVKNEP MLASFASTST NGKAKRSAKP SLFSGSEVSS DDDEEKPLAK RPKVEDSDSD 

        70         80         90        100        110        120 
APLTSTVSSQ NGVQKRSGSS NNDDNDDDSD SDSDAPLTAL VKKSNGSDDD EDDDDDDDEG 

       130        140        150        160        170        180 
DDDDEEDDDD DDDDDDKPLS KSSKSNRKPP KTMSITGSGE KKWDVLIHKG PRFPDPYQPL 

       190        200        210        220        230        240 
PKDVKLKYDG RPVDLPWQTE EIAMFYAVKL ETQHAQNAIF NRNFFDDFKT DLKKYPPRDG 

       250        260        270        280        290        300 
TQIKSFDKLD FRDMYNYWRS LKDAELERKK ALAPSARKRE IEERKAEETK WKICLVDGVE 

       310        320        330        340        350        360 
QRVGNVNVEP PGLFLGRGAH PKAGKVKRRI SPGDITINHS ADHPAPQPPA GMGDWAEVVE 

       370        380        390        400        410        420 
KKDVTWLAYW KENINGQYKY VFLDATSNFK TNSDREKFEK ARKLDTVVKQ IRRDVNKNLK 

       430        440        450        460        470        480 
SKVRHERQIA TIVWLIDNFS LRAGNEKGED EAETYGVCSL RCEHAQIKMP DTIHLEFLGK 

       490        500        510        520        530        540 
DSMKFEEDLK ITNPDVFKNI AMFLKSNGMK DKSGNYVRKK PSDPIFCAPE SGSGKMQPLQ 

       550        560        570        580        590        600 
PNSVNQFLSK YMKGLSAKVF RTYNASVTFQ GLLEQTEEWL KSRPNAAERE INQTNLRLAY 

       610        620        630        640        650        660 
NEANRQVAIL CNHQKTVNPM LLNRNLERTQ DKIFQIRYEI MKEQQKILTF HKVSELKKEF 

       670        680        690        700        710        720 
KVKEHPFMKQ FDKIMQKQDL DAEKVKQYEE QMISDKKSKL ESTFKRQQSE LQYQLEQKGL 

       730        740        750        760        770        780 
TGDDGTPKKG KKAKNVEEEV RSSIKGFKDK KQVDEELKAL NETAKRLEKE RKTNKSQATS 

       790        800        810        820        830        840 
CNFVSSAKKI LSKYEMIKKQ EAELVNKNNT SDVALSTSKL NYIDPRITLA WLKEWDDRLS 

       850        860        870        880        890        900 
DLGQGKAAPK KKVKKEEEEN DIKPKKKDAK GAASKKRAAK TGLANSTGDS EKMELGLQVM 

       910        920        930        940        950        960 
NISQFFANAL QKKFKWAASG DDGRDISAKW VFVKDAQSKM RKLDSAERKG QKGGSMAAMT 

       970        980        990       1000       1010 
DAADSKEAQP KVNCVLKKQT SADRKMSKPI KAVDKTEESD DDLSSDSSDD EDKPLAAVV 

« Hide

References

« Hide 'large scale' references
[1]"The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype."
Gerhold D., Thiyagarajan M., Kmiec E.B.
Nucleic Acids Res. 22:3773-3778(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 90884 / UCM005.
[2]"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. expand/collapse author list , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 521 / FGSC 9021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L32017 Genomic DNA. Translation: AAC37415.1.
AACP01000188 Genomic DNA. Translation: EAK82975.1.
PIRS53694.
RefSeqXP_761248.1. XM_756155.1.

3D structure databases

ProteinModelPortalP41511.
SMRP41511. Positions 812-837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5270.UM05101.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiUM05101T0; UM05101P0; UM05101.
GeneID3633275.
KEGGuma:UM05101.1.

Phylogenomic databases

eggNOGCOG3569.
KOK03163.
OrthoDBEOG7966R0.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 3 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_USTMA
AccessionPrimary (citable) accession number: P41511
Secondary accession number(s): Q4P462
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 12, 2006
Last modified: July 9, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families