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P41511

- TOP1_USTMA

UniProt

P41511 - TOP1_USTMA

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Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei317 – 3171Interaction with DNABy similarity
Sitei366 – 3661Interaction with DNABy similarity
Sitei397 – 3971Interaction with DNABy similarity
Sitei454 – 4541Interaction with DNABy similarity
Sitei480 – 4801Interaction with DNABy similarity
Sitei545 – 5451Interaction with DNABy similarity
Sitei613 – 6131Interaction with DNABy similarity
Sitei632 – 6321Interaction with DNABy similarity
Active sitei822 – 8221O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
ORF Names:UM05101
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
ProteomesiUP000000561: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10191019DNA topoisomerase 1PRO_0000145210Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi5270.UM05101.1.

Structurei

3D structure databases

ProteinModelPortaliP41511.
SMRiP41511. Positions 812-837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni379 – 3802Interaction with DNABy similarity
Regioni442 – 4476Interaction with DNABy similarity
Regioni556 – 5583Interaction with DNABy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 11811Poly-AspAdd
BLAST
Compositional biasi121 – 1244Poly-Asp
Compositional biasi127 – 13610Poly-Asp
Compositional biasi856 – 8594Poly-Glu

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

eggNOGiCOG3569.
InParanoidiP41511.
KOiK03163.
OrthoDBiEOG7966R0.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 3 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41511 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSIQVKNEP MLASFASTST NGKAKRSAKP SLFSGSEVSS DDDEEKPLAK
60 70 80 90 100
RPKVEDSDSD APLTSTVSSQ NGVQKRSGSS NNDDNDDDSD SDSDAPLTAL
110 120 130 140 150
VKKSNGSDDD EDDDDDDDEG DDDDEEDDDD DDDDDDKPLS KSSKSNRKPP
160 170 180 190 200
KTMSITGSGE KKWDVLIHKG PRFPDPYQPL PKDVKLKYDG RPVDLPWQTE
210 220 230 240 250
EIAMFYAVKL ETQHAQNAIF NRNFFDDFKT DLKKYPPRDG TQIKSFDKLD
260 270 280 290 300
FRDMYNYWRS LKDAELERKK ALAPSARKRE IEERKAEETK WKICLVDGVE
310 320 330 340 350
QRVGNVNVEP PGLFLGRGAH PKAGKVKRRI SPGDITINHS ADHPAPQPPA
360 370 380 390 400
GMGDWAEVVE KKDVTWLAYW KENINGQYKY VFLDATSNFK TNSDREKFEK
410 420 430 440 450
ARKLDTVVKQ IRRDVNKNLK SKVRHERQIA TIVWLIDNFS LRAGNEKGED
460 470 480 490 500
EAETYGVCSL RCEHAQIKMP DTIHLEFLGK DSMKFEEDLK ITNPDVFKNI
510 520 530 540 550
AMFLKSNGMK DKSGNYVRKK PSDPIFCAPE SGSGKMQPLQ PNSVNQFLSK
560 570 580 590 600
YMKGLSAKVF RTYNASVTFQ GLLEQTEEWL KSRPNAAERE INQTNLRLAY
610 620 630 640 650
NEANRQVAIL CNHQKTVNPM LLNRNLERTQ DKIFQIRYEI MKEQQKILTF
660 670 680 690 700
HKVSELKKEF KVKEHPFMKQ FDKIMQKQDL DAEKVKQYEE QMISDKKSKL
710 720 730 740 750
ESTFKRQQSE LQYQLEQKGL TGDDGTPKKG KKAKNVEEEV RSSIKGFKDK
760 770 780 790 800
KQVDEELKAL NETAKRLEKE RKTNKSQATS CNFVSSAKKI LSKYEMIKKQ
810 820 830 840 850
EAELVNKNNT SDVALSTSKL NYIDPRITLA WLKEWDDRLS DLGQGKAAPK
860 870 880 890 900
KKVKKEEEEN DIKPKKKDAK GAASKKRAAK TGLANSTGDS EKMELGLQVM
910 920 930 940 950
NISQFFANAL QKKFKWAASG DDGRDISAKW VFVKDAQSKM RKLDSAERKG
960 970 980 990 1000
QKGGSMAAMT DAADSKEAQP KVNCVLKKQT SADRKMSKPI KAVDKTEESD
1010
DDLSSDSSDD EDKPLAAVV
Length:1,019
Mass (Da):115,545
Last modified:December 12, 2006 - v2
Checksum:i7E89EDE7201E7FA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811P → A in AAC37415. (PubMed:7937091)Curated
Sequence conflicti197 – 1971W → C in AAC37415. (PubMed:7937091)Curated
Sequence conflicti341 – 3411A → G in AAC37415. (PubMed:7937091)Curated
Sequence conflicti434 – 4341W → C in AAC37415. (PubMed:7937091)Curated
Sequence conflicti452 – 4521A → T in AAC37415. (PubMed:7937091)Curated
Sequence conflicti1011 – 10111E → G in AAC37415. (PubMed:7937091)Curated
Sequence conflicti1017 – 10171A → S in AAC37415. (PubMed:7937091)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L32017 Genomic DNA. Translation: AAC37415.1.
AACP01000188 Genomic DNA. Translation: EAK82975.1.
PIRiS53694.
RefSeqiXP_761248.1. XM_756155.1.

Genome annotation databases

EnsemblFungiiUM05101T0; UM05101P0; UM05101.
GeneIDi3633275.
KEGGiuma:UM05101.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L32017 Genomic DNA. Translation: AAC37415.1 .
AACP01000188 Genomic DNA. Translation: EAK82975.1 .
PIRi S53694.
RefSeqi XP_761248.1. XM_756155.1.

3D structure databases

ProteinModelPortali P41511.
SMRi P41511. Positions 812-837.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5270.UM05101.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii UM05101T0 ; UM05101P0 ; UM05101 .
GeneIDi 3633275.
KEGGi uma:UM05101.1.

Phylogenomic databases

eggNOGi COG3569.
InParanoidi P41511.
KOi K03163.
OrthoDBi EOG7966R0.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 3 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype."
    Gerhold D., Thiyagarajan M., Kmiec E.B.
    Nucleic Acids Res. 22:3773-3778(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90884 / UCM005.
  2. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiTOP1_USTMA
AccessioniPrimary (citable) accession number: P41511
Secondary accession number(s): Q4P462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3