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P41511

- TOP1_USTMA

UniProt

P41511 - TOP1_USTMA

Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei317 – 3171Interaction with DNABy similarity
    Sitei366 – 3661Interaction with DNABy similarity
    Sitei397 – 3971Interaction with DNABy similarity
    Sitei454 – 4541Interaction with DNABy similarity
    Sitei480 – 4801Interaction with DNABy similarity
    Sitei545 – 5451Interaction with DNABy similarity
    Sitei613 – 6131Interaction with DNABy similarity
    Sitei632 – 6321Interaction with DNABy similarity
    Active sitei822 – 8221O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Gene namesi
    Name:TOP1
    ORF Names:UM05101
    OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
    Taxonomic identifieri237631 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
    ProteomesiUP000000561: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10191019DNA topoisomerase 1PRO_0000145210Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi5270.UM05101.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP41511.
    SMRiP41511. Positions 812-837.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni379 – 3802Interaction with DNABy similarity
    Regioni442 – 4476Interaction with DNABy similarity
    Regioni556 – 5583Interaction with DNABy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi108 – 11811Poly-AspAdd
    BLAST
    Compositional biasi121 – 1244Poly-Asp
    Compositional biasi127 – 13610Poly-Asp
    Compositional biasi856 – 8594Poly-Glu

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Phylogenomic databases

    eggNOGiCOG3569.
    KOiK03163.
    OrthoDBiEOG7966R0.

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 3 hits.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41511-1 [UniParc]FASTAAdd to Basket

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    MNSIQVKNEP MLASFASTST NGKAKRSAKP SLFSGSEVSS DDDEEKPLAK     50
    RPKVEDSDSD APLTSTVSSQ NGVQKRSGSS NNDDNDDDSD SDSDAPLTAL 100
    VKKSNGSDDD EDDDDDDDEG DDDDEEDDDD DDDDDDKPLS KSSKSNRKPP 150
    KTMSITGSGE KKWDVLIHKG PRFPDPYQPL PKDVKLKYDG RPVDLPWQTE 200
    EIAMFYAVKL ETQHAQNAIF NRNFFDDFKT DLKKYPPRDG TQIKSFDKLD 250
    FRDMYNYWRS LKDAELERKK ALAPSARKRE IEERKAEETK WKICLVDGVE 300
    QRVGNVNVEP PGLFLGRGAH PKAGKVKRRI SPGDITINHS ADHPAPQPPA 350
    GMGDWAEVVE KKDVTWLAYW KENINGQYKY VFLDATSNFK TNSDREKFEK 400
    ARKLDTVVKQ IRRDVNKNLK SKVRHERQIA TIVWLIDNFS LRAGNEKGED 450
    EAETYGVCSL RCEHAQIKMP DTIHLEFLGK DSMKFEEDLK ITNPDVFKNI 500
    AMFLKSNGMK DKSGNYVRKK PSDPIFCAPE SGSGKMQPLQ PNSVNQFLSK 550
    YMKGLSAKVF RTYNASVTFQ GLLEQTEEWL KSRPNAAERE INQTNLRLAY 600
    NEANRQVAIL CNHQKTVNPM LLNRNLERTQ DKIFQIRYEI MKEQQKILTF 650
    HKVSELKKEF KVKEHPFMKQ FDKIMQKQDL DAEKVKQYEE QMISDKKSKL 700
    ESTFKRQQSE LQYQLEQKGL TGDDGTPKKG KKAKNVEEEV RSSIKGFKDK 750
    KQVDEELKAL NETAKRLEKE RKTNKSQATS CNFVSSAKKI LSKYEMIKKQ 800
    EAELVNKNNT SDVALSTSKL NYIDPRITLA WLKEWDDRLS DLGQGKAAPK 850
    KKVKKEEEEN DIKPKKKDAK GAASKKRAAK TGLANSTGDS EKMELGLQVM 900
    NISQFFANAL QKKFKWAASG DDGRDISAKW VFVKDAQSKM RKLDSAERKG 950
    QKGGSMAAMT DAADSKEAQP KVNCVLKKQT SADRKMSKPI KAVDKTEESD 1000
    DDLSSDSSDD EDKPLAAVV 1019
    Length:1,019
    Mass (Da):115,545
    Last modified:December 12, 2006 - v2
    Checksum:i7E89EDE7201E7FA2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811P → A in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti197 – 1971W → C in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti341 – 3411A → G in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti434 – 4341W → C in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti452 – 4521A → T in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti1011 – 10111E → G in AAC37415. (PubMed:7937091)Curated
    Sequence conflicti1017 – 10171A → S in AAC37415. (PubMed:7937091)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32017 Genomic DNA. Translation: AAC37415.1.
    AACP01000188 Genomic DNA. Translation: EAK82975.1.
    PIRiS53694.
    RefSeqiXP_761248.1. XM_756155.1.

    Genome annotation databases

    EnsemblFungiiUM05101T0; UM05101P0; UM05101.
    GeneIDi3633275.
    KEGGiuma:UM05101.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32017 Genomic DNA. Translation: AAC37415.1 .
    AACP01000188 Genomic DNA. Translation: EAK82975.1 .
    PIRi S53694.
    RefSeqi XP_761248.1. XM_756155.1.

    3D structure databases

    ProteinModelPortali P41511.
    SMRi P41511. Positions 812-837.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5270.UM05101.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii UM05101T0 ; UM05101P0 ; UM05101 .
    GeneIDi 3633275.
    KEGGi uma:UM05101.1.

    Phylogenomic databases

    eggNOGi COG3569.
    KOi K03163.
    OrthoDBi EOG7966R0.

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 3 hits.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype."
      Gerhold D., Thiyagarajan M., Kmiec E.B.
      Nucleic Acids Res. 22:3773-3778(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 90884 / UCM005.
    2. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
      Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
      , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
      Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 521 / FGSC 9021.

    Entry informationi

    Entry nameiTOP1_USTMA
    AccessioniPrimary (citable) accession number: P41511
    Secondary accession number(s): Q4P462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3