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P41510 (PME_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable pectinesterase/pectinesterase inhibitor

Including the following 2 domains:

  1. Pectinesterase inhibitor
    Alternative name(s):
    Pectin methylesterase inhibitor
  2. Pectinesterase
    Short name=PE
    EC=3.1.1.11
    Alternative name(s):
    Pectin methylesterase
Gene names
Name:BP19
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall Probable.

Tissue specificity

Pollen, and at much lower levels in pistils and petals.

Developmental stage

Highly expressed during microspore development.

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Sequence similarities

In the N-terminal section; belongs to the PMEI family.

In the C-terminal section; belongs to the pectinesterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 584562Probable pectinesterase/pectinesterase inhibitor
PRO_0000023479

Regions

Region40 – 191152Pectinesterase inhibitor
Region272 – 571300Pectinesterase
Compositional bias258 – 26710Poly-Gly

Sites

Active site4021Proton donor; for pectinesterase activity By similarity
Active site4231Nucleophile; for pectinesterase activity By similarity
Binding site3491Substrate; for pectinesterase activity By similarity
Binding site3791Substrate; for pectinesterase activity By similarity
Binding site4921Substrate; for pectinesterase activity By similarity
Binding site4941Substrate; for pectinesterase activity By similarity
Site4011Transition state stabilizer By similarity

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation1051N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P41510 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 643887AA7F3AE6CE

FASTA58462,939
        10         20         30         40         50         60 
MAVGKIVISV ASMLLVVGVA IGVVTFVNKG GGAGGDKTLN SHQKAVESLC ASATDKGSCA 

        70         80         90        100        110        120 
KTLDPVKSDD PSKLIKAFML ATKDAVTKST NFTASTEEGM GKNINATSKA VLDYCKRVLM 

       130        140        150        160        170        180 
YALEDLETIV EEMGEDLQQS GSKMDQLKQW LTGVFNYQTD CIDDIEESEL RKVMGEGIAH 

       190        200        210        220        230        240 
SKILSSNAID IFHALTTAMS QMNVKVDDMK KGNLGETPAP DRDLLEDLDQ KGLPKWHSDK 

       250        260        270        280        290        300 
DRKLMAQAGR PGAPADEGIG EGGGGGGKIK PTHVVAKDGS GQFKTISEAV KACPEKNPGR 

       310        320        330        340        350        360 
CIIYIKAGVY KEQVTIPKKV NNVFMFGDGA TQTIITFDRS VGLSPGTTTS LSGTVQVESE 

       370        380        390        400        410        420 
GFMAKWIGFQ NTAGPLGHQA VAFRVNGDRA VIFNCRFDGY QDTLYVNNGR QFYRNIVVSG 

       430        440        450        460        470        480 
TVDFIFGKSA TVIQNSLILC RKGSPGQTNH VTADGNEKGK AVKIGIVLHN CRIMADKELE 

       490        500        510        520        530        540 
ADRLTVKSYL GRPWKPFATT AVIGTEIGDL IQPTGWNEWQ GEKFHLTATY VEFNNRGPGA 

       550        560        570        580 
NTAARVPWAK MAKSAAEVER FTVANWLTPA NWIQEANVPV QLGL

« Hide

References

[1]"A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5' flanking region are conserved in other pollen-specific promoters."
Albani D., Altosaar I., Arnison P.G., Fabijanski S.F.
Plant Mol. Biol. 16:501-513(1991) [PubMed: 1868195] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Westar.
Tissue: Pollen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X56195 Genomic DNA. Translation: CAA39658.1.
PIRS14952.

3D structure databases

ProteinModelPortalP41510.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
PfamPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
SSF101148. Pectinesterase_inhib. 1 hit.
TIGRFAMsTIGR01614. PME_inhib. 1 hit.
PROSITEPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePME_BRANA
AccessionPrimary (citable) accession number: P41510
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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