P41500 (ACYP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acylphosphatase-1
EC=3.6.1.7

Alternative name(s):
Acylphosphatase, erythrocyte/testis isozyme
Acylphosphatase, organ-common type isozyme
Acylphosphate phosphohydrolase 1

Gene names

Name:	ACYP1
Synonyms:	ACYPE

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	An acylphosphate + H2O = a carboxylate + phosphate.
Tissue specificity	Organ-common type isozyme is found in many different tissues.
Sequence similarities	Belongs to the acylphosphatase family. Contains 1 acylphosphatase-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Molecular function	Hydrolase
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular_function	acylphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Note: It is uncertain whether isoform ACY1 is produced by alternative initiation or another biological event.

Isoform ACY2 (identifier: P41500-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform ACY1 (identifier: P41500-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; in isoform ACY2 Ref.2 Ref.3

Chain

2 – 101

100

Acylphosphatase-1

PRO_0000158534

Regions

Domain

11 – 101

91

Acylphosphatase-like

Sites

Active site

26

1

Potential

Active site

44

1

Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine; in isoform ACY2 Ref.2

Natural variations

Alternative sequence

1 – 2

2

Missing in isoform ACY1.

VSP_026023

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Beta strand

9 – 19

11

Beta strand

21 – 24

4

Helix

25 – 35

11

Beta strand

39 – 44

6

Beta strand

50 – 57

8

Helix

58 – 70

13

Beta strand

77 – 88

12

Beta strand

95 – 100

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform ACY2 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 92DC05E7BF0D86B7	FASTA	101	11,432
	10 20 30 40 50 60 MSMAEGDTLI SVDYEIFGKV QGVFFRKYTQ AEGKKLGLVG WVQNTDQGTV QGQLQGPASK 70 80 90 100 VRHMQEWLET KGSPKSHIDR ASFHNEKVIV KLDYTDFQIV K
	Isoform ACY1 [UniParc]. Checksum: 73DBBF3C27569C06	FASTA	99	11,214
	10 20 30 40 50 60 MAEGDTLISV DYEIFGKVQG VFFRKYTQAE GKKLGLVGWV QNTDQGTVQG QLQGPASKVR 70 80 90 HMQEWLETKG SPKSHIDRAS FHNEKVIVKL DYTDFQIVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ACY2). Strain: Hereford. Tissue: Fetal pons.
[2]	"Purification, kinetic properties and primary structure of bovine erythrocyte acylphosphatase." Pazzagli L., Ikram U.K., Liguri G., Taddei N., Gentilini A., Cecchi C., Manao G., Cappugi G. Ital. J. Biochem. 42:233-245(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), ACETYLATION AT SER-2. Tissue: Erythrocyte.
[3]	"Bovine testis acylphosphatase: purification and amino acid sequence." Pazzagli L., Cappugi G., Camici G., Manao G., Ramponi G. J. Protein Chem. 12:593-601(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), PROTEIN SEQUENCE OF 4-101 (ISOFORM ACY1), ACETYLATION OF N-TERMINUS. Tissue: Testis.
[4]	"Crystal structure of common type acylphosphatase from bovine testis." Thunnissen M.M.G.M., Taddei N., Liguri G., Ramponi G., Nordlund P. Structure 5:69-79(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 4-101 (ISOFORM ACY1). Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC114901 mRNA. Translation: AAI14902.1.

IPI

IPI00708030.
IPI00852566.

PIR

A61449.

RefSeq

NP_001039447.1. NM_001045982.2.

UniGene

Bt.33942.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ACY	X-ray	1.80	A	4-101	[»]

ProteinModelPortal

P41500.

SMR

P41500. Positions 4-101.

ModBase

Search...

Protein-protein interaction databases

STRING

9913.ENSBTAP00000018595.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000018595; ENSBTAP00000018595; ENSBTAG00000013992.

GeneID

507844.

KEGG

bta:507844.

Organism-specific databases

CTD

97.

Phylogenomic databases

eggNOG

NOG289322.

GeneTree

ENSGT00390000011103.

HOGENOM

HOG000292688.

HOVERGEN

HBG050454.

InParanoid

P41500.

KO

K01512.

OMA

WLETKGS.

OrthoDB

EOG4XD3SN.

Enzyme and pathway databases

SABIO-RK

P41500.

Family and domain databases

InterPro

IPR020456. Acylphosphatase.
IPR001792. Acylphosphatase-like.
IPR017968. Acylphosphatase_CS.
[Graphical view]

Pfam

PF00708. Acylphosphatase. 1 hit.
[Graphical view]

PRINTS

PR00112. ACYLPHPHTASE.

SUPFAM

SSF54975. Acylphosphatase. 1 hit.

PROSITE

PS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P41500.

NextBio

20868244.

Entry information

Entry name

ACYP1_BOVIN

Accession

Primary (citable) accession number: P41500
Secondary accession number(s): Q1RMH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program