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Protein

Acylphosphatase-1

Gene

ACYP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An acylphosphate + H2O = a carboxylate + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261PROSITE-ProRule annotation
Active sitei44 – 441PROSITE-ProRule annotation

GO - Molecular functioni

  1. acylphosphatase activity Source: GO_Central

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

SABIO-RKP41500.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylphosphatase-1 (EC:3.6.1.7)
Alternative name(s):
Acylphosphatase, erythrocyte/testis isozyme
Acylphosphatase, organ-common type isozyme
Acylphosphate phosphohydrolase 1
Gene namesi
Name:ACYP1
Synonyms:ACYPE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 10

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; in isoform ACY21 Publication
Chaini2 – 101100Acylphosphatase-1PRO_0000158534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine; in isoform ACY21 Publication

Keywords - PTMi

Acetylation

Expressioni

Tissue specificityi

Organ-common type isozyme is found in many different tissues.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018595.

Structurei

Secondary structure

1
101
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 1911Combined sources
Beta strandi21 – 244Combined sources
Helixi25 – 3511Combined sources
Beta strandi39 – 446Combined sources
Beta strandi50 – 578Combined sources
Helixi58 – 7013Combined sources
Beta strandi77 – 8812Combined sources
Beta strandi95 – 1006Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ACYX-ray1.80A4-101[»]
ProteinModelPortaliP41500.
SMRiP41500. Positions 4-101.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41500.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 10191Acylphosphatase-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the acylphosphatase family.Curated
Contains 1 acylphosphatase-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG289322.
GeneTreeiENSGT00390000011103.
HOGENOMiHOG000292688.
HOVERGENiHBG050454.
InParanoidiP41500.
KOiK01512.
OMAiVIVKLDY.
OrthoDBiEOG7ZGX55.
TreeFamiTF300288.

Family and domain databases

InterProiIPR020456. Acylphosphatase.
IPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
[Graphical view]
PRINTSiPR00112. ACYLPHPHTASE.
SUPFAMiSSF54975. SSF54975. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Note: It is uncertain whether isoform ACY1 is produced by alternative initiation or another biological event.

Isoform ACY2 (identifier: P41500-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSMAEGDTLI SVDYEIFGKV QGVFFRKYTQ AEGKKLGLVG WVQNTDQGTV
60 70 80 90 100
QGQLQGPASK VRHMQEWLET KGSPKSHIDR ASFHNEKVIV KLDYTDFQIV

K
Length:101
Mass (Da):11,432
Last modified:January 23, 2007 - v2
Checksum:i92DC05E7BF0D86B7
GO
Isoform ACY1 (identifier: P41500-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2.

Show »
Length:99
Mass (Da):11,214
Checksum:i73DBBF3C27569C06
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 22Missing in isoform ACY1. CuratedVSP_026023

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114901 mRNA. Translation: AAI14902.1.
PIRiA61449.
RefSeqiNP_001039447.1. NM_001045982.2. [P41500-1]
XP_005212005.1. XM_005211948.1. [P41500-1]
UniGeneiBt.33942.

Genome annotation databases

EnsembliENSBTAT00000018595; ENSBTAP00000018595; ENSBTAG00000013992. [P41500-1]
GeneIDi507844.
KEGGibta:507844.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC114901 mRNA. Translation: AAI14902.1.
PIRiA61449.
RefSeqiNP_001039447.1. NM_001045982.2. [P41500-1]
XP_005212005.1. XM_005211948.1. [P41500-1]
UniGeneiBt.33942.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ACYX-ray1.80A4-101[»]
ProteinModelPortaliP41500.
SMRiP41500. Positions 4-101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000018595; ENSBTAP00000018595; ENSBTAG00000013992. [P41500-1]
GeneIDi507844.
KEGGibta:507844.

Organism-specific databases

CTDi97.

Phylogenomic databases

eggNOGiNOG289322.
GeneTreeiENSGT00390000011103.
HOGENOMiHOG000292688.
HOVERGENiHBG050454.
InParanoidiP41500.
KOiK01512.
OMAiVIVKLDY.
OrthoDBiEOG7ZGX55.
TreeFamiTF300288.

Enzyme and pathway databases

SABIO-RKP41500.

Miscellaneous databases

EvolutionaryTraceiP41500.
NextBioi20868244.

Family and domain databases

InterProiIPR020456. Acylphosphatase.
IPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
[Graphical view]
PRINTSiPR00112. ACYLPHPHTASE.
SUPFAMiSSF54975. SSF54975. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ACY2).
    Strain: Hereford.
    Tissue: Fetal pons.
  2. "Purification, kinetic properties and primary structure of bovine erythrocyte acylphosphatase."
    Pazzagli L., Ikram U.K., Liguri G., Taddei N., Gentilini A., Cecchi C., Manao G., Cappugi G.
    Ital. J. Biochem. 42:233-245(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), ACETYLATION AT SER-2.
    Tissue: Erythrocyte.
  3. "Bovine testis acylphosphatase: purification and amino acid sequence."
    Pazzagli L., Cappugi G., Camici G., Manao G., Ramponi G.
    J. Protein Chem. 12:593-601(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-101 (ISOFORM ACY2), PROTEIN SEQUENCE OF 4-101 (ISOFORM ACY1), ACETYLATION OF N-TERMINUS.
    Tissue: Testis.
  4. "Crystal structure of common type acylphosphatase from bovine testis."
    Thunnissen M.M.G.M., Taddei N., Liguri G., Ramponi G., Nordlund P.
    Structure 5:69-79(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 4-101 (ISOFORM ACY1).
    Tissue: Testis.

Entry informationi

Entry nameiACYP1_BOVIN
AccessioniPrimary (citable) accession number: P41500
Secondary accession number(s): Q1RMH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.