Reviewed, UniProtKB/Swiss-Prot P41498 (PPAC_RAT)

Names and origin

Protein names

Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase
      Short name=LMW-PTPase
      Short name=LMW-PTP
    EC=3.1.3.48
Alternative name(s):
    Low molecular weight cytosolic acid phosphatase
    EC=3.1.3.2

Gene names

Name:

Acp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphate monoester + H2O = an alcohol + phosphate.
Subunit structure	Isoform 1 interacts with the SH3 domain of SPTAN1. Ref.1
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro synaptic transmission Inferred from expression pattern. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from direct assay. Source: RGD
Molecular function	SH3 domain binding Ref.1 Inferred from direct assay. Source: RGD acid phosphatase activity Inferred from direct assay. Source: RGD non-membrane spanning protein tyrosine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P41498-1)

Also known as: ACP1; A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P41498-2)

Also known as: ACP2; B;

The sequence of this isoform differs from the canonical sequence as follows:
     41-74: RIDSAATSTYEVGNPPDYRGQNCMKKHGIHMQHI → AIDSSAVSDWNVGRPPDPRAVNCLRNHGISTAHK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 158	157	Low molecular weight phosphotyrosine protein phosphatase		PRO_0000046561
Sites
	Active site	13	1	Nucleophile By similarity
	Active site	19	1	By similarity
	Active site	130	1	Proton donor By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
	Modified residue	132	1	Phosphotyrosine By similarity
	Modified residue	133	1	Phosphotyrosine By similarity
	Modified residue	156	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	41 – 74	34	RIDSA…HMQHI → AIDSSAVSDWNVGRPPDPRA VNCLRNHGISTAHK in isoform 2.		VSP_004705
Experimental info
	Sequence conflict	2	1	A → ACA AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ACP1) (A) [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5C454D4DA82FB094	FASTA	158	18,152
	10 20 30 40 50 60 MAEVGSKSVL FVCLGNICRS PIAEAVFRKL VTDENVSDNW RIDSAATSTY EVGNPPDYRG 70 80 90 100 110 120 QNCMKKHGIH MQHIARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY 130 140 150 DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH
	Isoform 2 (ACP2) (B). Checksum: 9BA17AFBC38AC8F6	FASTA	158	17,937
	10 20 30 40 50 60 MAEVGSKSVL FVCLGNICRS PIAEAVFRKL VTDENVSDNW AIDSSAVSDW NVGRPPDPRA 70 80 90 100 110 120 VNCLRNHGIS TAHKARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY 130 140 150 DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." Nicolas G., Fournier C.M., Galand C., Malbert-Colas L., Bournier O., Kroviarski Y., Bourgeois M., Camonis J.H., Dhermy D., Grandchamp B., Lecomte M.-C. Mol. Cell. Biol. 22:3527-3536(2002) [PubMed: 11971983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SPTAN1. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"Rat liver low M(r) phosphotyrosine protein phosphatase isoenzymes: purification and amino acid sequences." Manao G., Pazzagli L., Cirri P., Caselli A., Camici G., Cappugi G., Saeed A., Ramponi G. J. Protein Chem. 11:333-345(1992) [PubMed: 1388675] [Abstract] Cited for: PROTEIN SEQUENCE (ISOFORMS 1 AND 2). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary.
[4]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 20-28; 42-59 AND 114-124, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF171072 mRNA. Translation: AAD50990.1. BC062229 mRNA. Translation: AAH62229.1.
IPI	IPI00206664. IPI00231705.
PIR	A53874.
RefSeq	NP_067085.1.
UniGene	Rn.108187 Rn.202830
3D structure databases
SMR	P41498. Positions 2-158.
ModBase	Search...
Protein-protein interaction databases
STRING	P41498.
PTM databases
PhosphoSite	P41498.
Genome annotation databases
Ensembl	ENSRNOT00000007287; ENSRNOP00000007287; ENSRNOG00000005260; Rattus norvegicus. [Genome view]
GeneID	24161.
Organism-specific databases
CTD	24161.
RGD	2020. Acp1.
Phylogenomic databases
eggNOG	maNOG05136.
HOVERGEN	P41498.
InParanoid	P41498.
OMA	GAHTPDV.
OrthoDB	EOG9SJ80C.
PhylomeDB	P41498.
Enzyme and pathway databases
BRENDA	3.1.3.2. 248. 3.1.3.48. 248.
Gene expression databases
Genevestigator	P41498.
GermOnline	ENSRNOG00000005260. Rattus norvegicus.
Family and domain databases
InterPro	IPR002115. Tyr_Pase_low_mol_wt_mml. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE. PR00720. MAMMALPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602457.

Entry information

Entry name

PPAC_RAT

Accession

Primary (citable) accession number: P41498
Secondary accession number(s): Q9R138

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)