P41498 (PPAC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Low molecular weight phosphotyrosine protein phosphatase
Short name=LMW-PTP
Short name=LMW-PTPase
EC=3.1.3.48

Alternative name(s):
Low molecular weight cytosolic acid phosphatase
EC=3.1.3.2

Gene names

Name:

Acp1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphate monoester + H2O = an alcohol + phosphate.
Subunit structure	Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1 By similarity. Isoform 1 interacts with the SH3 domain of SPTAN1. Ref.1
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Inferred from expression pattern PubMed 10480315. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membrane Inferred from electronic annotation. Source: Compara neuron projection Inferred from direct assay PubMed 10480315. Source: RGD nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	SH3 domain binding Inferred from direct assay Ref.1. Source: RGD acid phosphatase activity Inferred from direct assay PubMed 7827101. Source: RGD non-membrane spanning protein tyrosine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from direct assay PubMed 7686862. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P41498-1)

Also known as: ACP1; A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P41498-2)

Also known as: ACP2; B;

The sequence of this isoform differs from the canonical sequence as follows:
     41-74: RIDSAATSTYEVGNPPDYRGQNCMKKHGIHMQHI → AIDSSAVSDWNVGRPPDPRAVNCLRNHGISTAHK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 158	157	Low molecular weight phosphotyrosine protein phosphatase		PRO_0000046561
Sites
	Active site	13	1	Nucleophile By similarity
	Active site	19	1	By similarity
	Active site	130	1	Proton donor By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
	Modified residue	132	1	Phosphotyrosine By similarity
	Modified residue	133	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	41 – 74	34	RIDSA…HMQHI → AIDSSAVSDWNVGRPPDPRA VNCLRNHGISTAHK in isoform 2.		VSP_004705
Experimental info
	Sequence conflict	2	1	A → ACA AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ACP1) (A) [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5C454D4DA82FB094	FASTA	158	18,152
	10 20 30 40 50 60 MAEVGSKSVL FVCLGNICRS PIAEAVFRKL VTDENVSDNW RIDSAATSTY EVGNPPDYRG 70 80 90 100 110 120 QNCMKKHGIH MQHIARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY 130 140 150 DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH
	Isoform 2 (ACP2) (B) [UniParc]. Checksum: 9BA17AFBC38AC8F6	FASTA	158	17,937
	10 20 30 40 50 60 MAEVGSKSVL FVCLGNICRS PIAEAVFRKL VTDENVSDNW AIDSSAVSDW NVGRPPDPRA 70 80 90 100 110 120 VNCLRNHGIS TAHKARQITR EDFATFDYIL CMDESNLRDL NRKSNQVKNC KAKIELLGSY 130 140 150 DPQKQLIIED PYYGNDSDFE VVYQQCLRCC KAFLEKTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." Nicolas G., Fournier C.M., Galand C., Malbert-Colas L., Bournier O., Kroviarski Y., Bourgeois M., Camonis J.H., Dhermy D., Grandchamp B., Lecomte M.-C. Mol. Cell. Biol. 22:3527-3536(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH SPTAN1. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"Rat liver low M(r) phosphotyrosine protein phosphatase isoenzymes: purification and amino acid sequences." Manao G., Pazzagli L., Cirri P., Caselli A., Camici G., Cappugi G., Saeed A., Ramponi G. J. Protein Chem. 11:333-345(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE (ISOFORMS 1 AND 2). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary.
[4]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 20-28; 42-59 AND 114-124, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF171072 mRNA. Translation: AAD50990.1. BC062229 mRNA. Translation: AAH62229.1.
IPI	IPI00206664. IPI00231705.
PIR	A53874.
RefSeq	NP_067085.1. NM_021262.2.
UniGene	Rn.108187. Rn.202830. Rn.220315.
3D structure databases
ProteinModelPortal	P41498.
SMR	P41498. Positions 2-158.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007287.
PTM databases
PhosphoSite	P41498.
Proteomic databases
PaxDb	P41498.
PRIDE	P41498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24161.
KEGG	rno:24161.
UCSC	RGD:2020. rat.
Organism-specific databases
CTD	52.
RGD	2020. Acp1.
Phylogenomic databases
eggNOG	COG0394.
HOGENOM	HOG000273094.
HOVERGEN	HBG007540.
InParanoid	P41498.
KO	K14394.
OMA	YEIGSPP.
OrthoDB	EOG4DR9DN.
Gene expression databases
Genevestigator	P41498.
GermOnline	ENSRNOG00000005260. Rattus norvegicus.
Family and domain databases
InterPro	IPR023485. Ptyr_pPase_SF. IPR002115. Tyr_Pase_low_mol_wt_mml. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. PTHR11717. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE. PR00720. MAMMALPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
SUPFAM	SSF52788. Tyr_Pase_low_mol_wt. 1 hit.
ProtoNet	Search...
Other
NextBio	602457.

Entry information

Entry name

PPAC_RAT

Accession

Primary (citable) accession number: P41498
Secondary accession number(s): Q9R138

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program