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Protein

Putative type II secretion system protein F

Gene

gspF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7705-MONOMER.
ECOL316407:JW3289-MONOMER.
MetaCyc:G7705-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type II secretion system protein F
Short name:
T2SS protein F
Alternative name(s):
General secretion pathway protein F
Protein transport protein HofF
Gene namesi
Name:gspF
Synonyms:hofF, hopF
Ordered Locus Names:b3327, JW3289
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12885. gspF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 163163PeriplasmicSequence analysisAdd
BLAST
Transmembranei164 – 18623HelicalSequence analysisAdd
BLAST
Topological domaini187 – 21933CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 23617HelicalSequence analysisAdd
BLAST
Topological domaini237 – 24812PeriplasmicSequence analysisAdd
BLAST
Transmembranei249 – 26517HelicalSequence analysisAdd
BLAST
Topological domaini266 – 35994CytoplasmicSequence analysisAdd
BLAST
Transmembranei360 – 38425HelicalSequence analysisAdd
BLAST
Topological domaini385 – 39814PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Putative type II secretion system protein FPRO_0000207831Add
BLAST

Proteomic databases

PaxDbiP41441.

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262168. 98 interactions.
STRINGi511145.b3327.

Structurei

3D structure databases

ProteinModelPortaliP41441.
SMRiP41441. Positions 56-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP F family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D7Q. Bacteria.
COG1459. LUCA.
HOGENOMiHOG000253739.
InParanoidiP41441.
KOiK02455.
OMAiFEPLMIL.
PhylomeDBiP41441.

Family and domain databases

InterProiIPR011850. GspF.
IPR003004. GspF/PilC.
IPR018076. T2SS_F.
IPR001992. Type_2_secretion_system_CS.
[Graphical view]
PfamiPF00482. T2SSF. 2 hits.
[Graphical view]
PRINTSiPR00812. BCTERIALGSPF.
TIGRFAMsiTIGR02120. GspF. 1 hit.
PROSITEiPS00874. T2SP_F. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYRYRAMTQ DGQKLQGIID ANDERQARLR LREEGLFLLD IRPQKSSGVK
60 70 80 90 100
TRRPRISHSE LTLFTRQLAT LSAAALPLEE SLAVIGQQSS NKRLGDVLNQ
110 120 130 140 150
VRSAILEGHP LSDALQHFPT LFDSLYRTLV KAGEKSGLLA PVLEKLADYN
160 170 180 190 200
ENRQKIRSKL IQSLIYPCML TTVAIGVVII LLTAVVPKIT EQFVHMKQQL
210 220 230 240 250
PLSTRILLGL SDTLQRTGPT LLATVFIVAV GFWLWLKRGN NRHRFHAMLL
260 270 280 290 300
RVALIGPLIC AINSARYLRT LSILQSSGVP LLDGMNLSTE SLNNLEIRQR
310 320 330 340 350
LANAAENVRQ GNSIHLSLEQ TAIFPPMMLY MVASGEKSGQ LGTLMVRAAD
360 370 380 390
NQETLQQNRI ALTLSIFEPA LIITMALIVL FIVVSVLQPL LQLNSMIN
Length:398
Mass (Da):44,339
Last modified:November 1, 1995 - v1
Checksum:i8A2696AC7948BC8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti270 – 2701T → P in AAA69030 (PubMed:7896718).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58124.1.
U00096 Genomic DNA. Translation: AAC76352.1.
AP009048 Genomic DNA. Translation: BAE77964.1.
U20786 Genomic DNA. Translation: AAA69030.1.
PIRiB65126.
RefSeqiNP_417786.1. NC_000913.3.
WP_001109573.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76352; AAC76352; b3327.
BAE77964; BAE77964; BAE77964.
GeneIDi947829.
KEGGiecj:JW3289.
eco:b3327.
PATRICi32122086. VBIEscCol129921_3420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58124.1.
U00096 Genomic DNA. Translation: AAC76352.1.
AP009048 Genomic DNA. Translation: BAE77964.1.
U20786 Genomic DNA. Translation: AAA69030.1.
PIRiB65126.
RefSeqiNP_417786.1. NC_000913.3.
WP_001109573.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP41441.
SMRiP41441. Positions 56-163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262168. 98 interactions.
STRINGi511145.b3327.

Proteomic databases

PaxDbiP41441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76352; AAC76352; b3327.
BAE77964; BAE77964; BAE77964.
GeneIDi947829.
KEGGiecj:JW3289.
eco:b3327.
PATRICi32122086. VBIEscCol129921_3420.

Organism-specific databases

EchoBASEiEB2722.
EcoGeneiEG12885. gspF.

Phylogenomic databases

eggNOGiENOG4105D7Q. Bacteria.
COG1459. LUCA.
HOGENOMiHOG000253739.
InParanoidiP41441.
KOiK02455.
OMAiFEPLMIL.
PhylomeDBiP41441.

Enzyme and pathway databases

BioCyciEcoCyc:G7705-MONOMER.
ECOL316407:JW3289-MONOMER.
MetaCyc:G7705-MONOMER.

Miscellaneous databases

PROiP41441.

Family and domain databases

InterProiIPR011850. GspF.
IPR003004. GspF/PilC.
IPR018076. T2SS_F.
IPR001992. Type_2_secretion_system_CS.
[Graphical view]
PfamiPF00482. T2SSF. 2 hits.
[Graphical view]
PRINTSiPR00812. BCTERIALGSPF.
TIGRFAMsiTIGR02120. GspF. 1 hit.
PROSITEiPS00874. T2SP_F. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSPF_ECOLI
AccessioniPrimary (citable) accession number: P41441
Secondary accession number(s): Q2M6Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.