Folate transporter 1 - Homo sapiens (Human)

Contribute

Send feedback

Reviewed, UniProtKB/Swiss-Prot P41440 (S19A1_HUMAN)

Last modified November 25, 2008. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Folate transporter 1
      Short name=FOLT
Alternative name(s):
    Reduced folate carrier protein
      Short name=RFC
    Intestinal folate carrier
    IFC-1
    Placental folate transporter
    Solute carrier family 19 member 1

Gene names

Name:	SLC19A1
Synonyms:	FLOT1, RFC1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Placenta, liver, and to a much smaller extent, in lung.
Post-translational modification	Heavily glycosylated.
Sequence similarities	Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family. [View classification]

Hide | Top

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	Folate-binding
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	folic acid transport Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Traceable author statement. Source: ProtInc
Molecular function	folic acid binding Inferred from electronic annotation. Source: InterPro folic acid transporter activity Ref.1 Traceable author statement. Source: ProtInc methotrexate transporter activity Traceable author statement. Source: ProtInc reduced folate carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 591

591

Folate transporter 1

PRO_0000178660

Regions

Topological domain

1 – 23

Cytoplasmic Probable

Transmembrane

24 – 44

Probable

Topological domain

45 – 70

Extracellular Probable

Transmembrane

71 – 89

Probable

Topological domain

90 – 95

Cytoplasmic Probable

Transmembrane

96 – 116

Probable

Topological domain

117 – 123

Extracellular Probable

Transmembrane

124 – 144

Probable

Topological domain

145 – 159

Cytoplasmic Probable

Transmembrane

160 – 177

Probable

Topological domain

178 – 186

Extracellular Probable

Transmembrane

187 – 203

Probable

Topological domain

204 – 259

Cytoplasmic Probable

Transmembrane

260 – 287

Probable

Topological domain

288 – 309

Extracellular Probable

Transmembrane

310 – 327

Probable

Topological domain

328 – 333

Cytoplasmic Probable

Transmembrane

334 – 354

Probable

Topological domain

355 – 360

Extracellular Probable

Transmembrane

361 – 378

Probable

Topological domain

379 – 394

Cytoplasmic Probable

Transmembrane

395 – 419

Probable

Topological domain

420 – 433

Extracellular Probable

Transmembrane

434 – 454

Probable

Topological domain

455 – 591

137

Cytoplasmic Probable

Amino acid modifications

Modified residue

583

Phosphoserine

Glycosylation

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

H → R: dbSNP rs1051266.

VAR_020210

Experimental info

Sequence conflict

62 – 63

EQ → DE Ref.1 Ref.4

Sequence conflict

268

R → P in AAA98442. Ref.1

Sequence conflict

457

H → D in AAB35058. Ref.4

Sequence conflict

483

A → R in AAB35058. Ref.4

Sequence conflict

549

C → S Ref.1 Ref.4 Ref.5 Ref.6

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P41440-1 [UniParc].

Last modified December 1, 2000. Version 3.
Checksum: 0437B1615F5517EB
Show »

FASTA

591

64,868

        10         20         30         40         50         60 
MVPSSPAVEK QVPVEPGPDP ELRSWRHLVC YLCFYGFMAQ IRPGESFITP YLLGPDKNFT 

        70         80         90        100        110        120 
REQVTNEITP VLSYSYLAVL VPVFLLTDYL RYTPVLLLQG LSFVSVWLLL LLGHSVAHMQ 

       130        140        150        160        170        180 
LMELFYSVTM AARIAYSSYI FSLVRPARYQ RVAGYSRAAV LLGVFTSSVL GQLLVTVGRV 

       190        200        210        220        230        240 
SFSTLNYISL AFLTFSVVLA LFLKRPKRSL FFNRDDRGRC ETSASELERM NPGPGGKLGH 

       250        260        270        280        290        300 
ALRVACGDSV LARMLRELGD SLRRPQLRLW SLWWVFNSAG YYLVVYYVHI LWNEVDPTTN 

       310        320        330        340        350        360 
SARVYNGAAD AASTLLGAIT SFAAGFVKIR WARWSKLLIA GVTATQAGLV FLLAHTRHPS 

       370        380        390        400        410        420 
SIWLCYAAFV LFRGSYQFLV PIATFQIASS LSKELCALVF GVNTFFATIV KTIITFIVSD 

       430        440        450        460        470        480 
VRGLGLPVRK QFQLYSVYFL ILSIIYFLGA MLDGLRHCQR GHHPRQPPAQ GLRSAAEEKA 

       490        500        510        520        530        540 
AQALSVQDKG LGGLQPAQSP PLSPEDSLGA VGPASLEQRQ SDPYLAQAPA PQAAEFLSPV 

       550        560        570        580        590 
TTPSPCTLCS AQASGPEAAD ETCPQLAVHP PGVSKLGLQC LPSDGVQNVN Q

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human placental folate transporter." Prasad P.D., Ramamoorthy S., Leibach F.H., Ganapathy V. Biochem. Biophys. Res. Commun. 206:681-687(1995) [PubMed: 7826387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-27. Tissue: Placenta.
[2]	Prasad P.D. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 490-527.
[3]	"Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells." Wong S.C., Proefke A., Bhushan A., Matherly L.H. J. Biol. Chem. 270:17468-17475(1995) [PubMed: 7615551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-27.
[4]	"Isolation of a gene encoding a human reduced folate carrier (RFC1) and analysis of its expression in transport-deficient, methotrexate-resistant human breast cancer cells." Moscow J.A., Gong M., He R., Sgagias M.K., Dixon K.H., Anzick S.L., Meltzer P.S., Cowan K.H. Cancer Res. 55:3790-3794(1995) [PubMed: 7641195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-27. Tissue: Testis.
[5]	"Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake." Williams F.M., Flintoff W.F. J. Biol. Chem. 270:2987-2992(1995) [PubMed: 7852378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma.
[6]	"Structural analysis of the human RFC1 gene encoding a folate transporter and alternatively spliced transcripts with 5' end heterogeneity." Tolner B.M., Roy K., Sirotnak F.M. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE, VARIANT ARG-27. Tissue: Liver.
[7]	"Human intestinal folate transport: cloning, expression, and distribution of complementary RNA." Nguyen T.T., Dyer D.L., Dunning D.D., Rubin S.A., Grant K.E., Said H.M. Gastroenterology 112:783-791(1997) [PubMed: 9041240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.
[8]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion." Ferguson P.L., Flintoff W.F. J. Biol. Chem. 274:16269-16278(1999) [PubMed: 10347183] [Abstract] Cited for: TOPOLOGY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-583, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	U15939 mRNA. Translation: AAA98442.1. U19720 mRNA. Translation: AAC50180.1. S78996 mRNA. Translation: AAB35058.1. U17566 mRNA. Translation: AAA74914.1. U92873 , U92869, U92870, U92871, U92872 Genomic DNA. Translation: AAC26162.1. AF004354 mRNA. Translation: AAB61417.1. AL163302 Genomic DNA. Translation: CAB90483.1. Sequence problems.
PIR	I38924. I52728.
RefSeq	NP_919231.1.
UniGene	Hs.84190
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P41440.
Genome annotation databases
Ensembl	ENSG00000173638. Homo sapiens. [Contig view]
GeneID	6573.
KEGG	hsa:6573.
Organism-specific databases
H-InvDB	HIX0016185.
HGNC	HGNC:10937. SLC19A1.
MIM	600424. gene.
PharmGKB	PA327.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P41440.
Enzyme and pathway databases
Reactome	REACT_11127. Metabolism of vitamins and cofactors.
Gene expression databases
ArrayExpress	P41440.
CleanEx	HS_FLOT1. HS_RFC1. HS_SLC19A1.
GermOnline	ENSG00000173638. Homo sapiens.
Family and domain databases
InterPro	IPR002666. Folate_carrier. [Graphical view]
PANTHER	PTHR10686. Folate_carrier. 1 hit.
Pfam	PF01770. Folate_carrier. 1 hit. [Graphical view]
PIRSF	PIRSF028739. Folate_carrier. 1 hit.
TIGRFAMs	TIGR00806. rfc. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	25575.
SOURCE	Search...

Hide | Top

Entry information

Entry name

S19A1_HUMAN

Accession

Primary (citable) accession number: P41440
Secondary accession number(s): O00553, O60227, Q13026

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents