Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P41438 (S19A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Folate transporter 1
Alternative name(s):
Intestinal folate carrier 1
Short name=IFC-1
Reduced folate carrier 1
Short name=RFC-1
Short name=RFC1
Solute carrier family 19 member 1
Gene names
Name:Slc19a1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter for the intake of folate, reduced folates and methotrexate.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P41438-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P41438-2)

The sequence of this isoform differs from the canonical sequence as follows:
     273-313: WWVFNSSGYYLITYYVHVLWRSTDSSLSYNGAVDAASTLLS → C
Isoform 3 (identifier: P41438-3)

The sequence of this isoform differs from the canonical sequence as follows:
     378-379: FQ → PS
     380-512: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Folate transporter 1
PRO_0000178661

Regions

Transmembrane28 – 4821Helical; Potential
Transmembrane65 – 8521Helical; Potential
Transmembrane92 – 11221Helical; Potential
Transmembrane122 – 14221Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane181 – 20121Helical; Potential
Transmembrane272 – 29221Helical; Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane330 – 35021Helical; Potential
Transmembrane355 – 37521Helical; Potential
Transmembrane391 – 41121Helical; Potential
Transmembrane427 – 44721Helical; Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue4671Phosphoserine Ref.6
Modified residue4771Phosphoserine Ref.6
Glycosylation2111N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence273 – 31341WWVFN…STLLS → C in isoform 2.
VSP_006125
Alternative sequence378 – 3792FQ → PS in isoform 3.
VSP_006126
Alternative sequence380 – 512133Missing in isoform 3.
VSP_006127

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 640CB7AD2624BF67

FASTA51258,150
        10         20         30         40         50         60 
MVPTGQVAEK QAYEEPRQDH ELKSWRCLVF YLCFFGFMAQ LRPGESFITP FLLERKFTKE 

        70         80         90        100        110        120 
QVTNEIIPML PYSHLAVLVP VFLLTDYLRY KPVLVLQCLS FVCVWLLLLL GTSVVHMQLM 

       130        140        150        160        170        180 
EVFYSVTMAA RIAYSSYIFS LVHPSRYQRM ASYSRAAVLL GVFISSVLGQ ALVTVGHIST 

       190        200        210        220        230        240 
YTLNCVSLGF ILFSLVLSLF LKRPKRSLFF NRSTLARGAL PCELDQMHPG PDRPETRKLD 

       250        260        270        280        290        300 
RMLGTCRDSF LVRMLSELVE NARQPQLRLW CLWWVFNSSG YYLITYYVHV LWRSTDSSLS 

       310        320        330        340        350        360 
YNGAVDAAST LLSAITSFSA GFLSIRWTLW SKLVIAGVIA IQASLVFCMF QIRDIWVCYV 

       370        380        390        400        410        420 
TFVLFRGAYQ FLVPIATFQI ASSLSKELCA LVFGINTFLA TALKTCITLV VSDKRGLGLQ 

       430        440        450        460        470        480 
VRDQFRIYFI YFLMLSITCF AWAGLDGLRY CQRGRHQPLA QAQELRSPLE TSVQAISLQD 

       490        500        510 
GDLRGPQPSA PQLLSEDGME DDRGDLRVEA KA 

« Hide

Isoform 2 [UniParc].

Checksum: 4BBD933AD971CE1D
Show »

FASTA47253,577
Isoform 3 [UniParc].

Checksum: 898147C34EB427B5
Show »

FASTA37943,371

References

« Hide 'large scale' references
[1]"A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells."
Dixon K.H., Lanpher B.C., Chiu J., Kelly K., Cowan K.H.
J. Biol. Chem. 269:17-20(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA.
[2]"Intestinal folate transport: identification of a cDNA involved in folate transport and the functional expression and distribution of its mRNA."
Said H.M., Nguyen T.T., Dyer D.L., Cowan K.H., Rubin S.A.
Biochim. Biophys. Acta 1281:164-172(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA/2.
Tissue: Intestine.
[3]"Characterization of a mutation in the reduced folate carrier in a transport defective L1210 murine leukemia cell line."
Brigle K.E., Spinella M.J., Sierra E.E., Goldman I.D.
J. Biol. Chem. 270:22974-22979(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: DBA/2.
[4]"Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter."
Tolner B.M., Roy K., Sirotnak F.M.
Gene 189:1-7(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 3).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467 AND SER-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36539 mRNA. Translation: AAB38483.1.
L23755 mRNA. Translation: AAA39738.1.
U32469 mRNA. Translation: AAC52258.1.
U66103 mRNA. Translation: AAC53287.1.
U57784 expand/collapse EMBL AC list , U57781, U57782, U57783 Genomic DNA. Translation: AAC53288.1.
U57785 expand/collapse EMBL AC list , U57781, U57782, U57783 Genomic DNA. Translation: AAC53289.1.
U57785 expand/collapse EMBL AC list , U57781, U57782, U57783, U57784 Genomic DNA. Translation: AAC53290.1.
U57785 expand/collapse EMBL AC list , U57781, U57782, U57783 Genomic DNA. Translation: AAC53291.1.
BC015263 mRNA. Translation: AAH15263.1.
PIRA53092.
RefSeqNP_001186200.1. NM_001199271.1.
NP_112473.1. NM_031196.3.
XP_006513477.1. XM_006513414.1.
XP_006513478.1. XM_006513415.1.
XP_006513479.1. XM_006513416.1.
XP_006513480.1. XM_006513417.1.
XP_006513481.1. XM_006513418.1.
UniGeneMm.265060.

3D structure databases

ProteinModelPortalP41438.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP41438.

Proteomic databases

PRIDEP41438.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000105410; ENSMUSP00000101050; ENSMUSG00000001436. [P41438-1]
ENSMUST00000144234; ENSMUSP00000116784; ENSMUSG00000001436. [P41438-1]
GeneID20509.
KEGGmmu:20509.
UCSCuc007fvc.2. mouse. [P41438-1]

Organism-specific databases

CTD6573.
MGIMGI:103182. Slc19a1.

Phylogenomic databases

eggNOGNOG241030.
HOGENOMHOG000001583.
HOVERGENHBG054198.
InParanoidP41438.
KOK14609.
PhylomeDBP41438.
TreeFamTF313684.

Gene expression databases

ArrayExpressP41438.
BgeeP41438.
GenevestigatorP41438.

Family and domain databases

InterProIPR002666. Folate_carrier.
IPR016196. MFS_dom_general_subst_transpt.
IPR028339. RFC.
[Graphical view]
PANTHERPTHR10686. PTHR10686. 1 hit.
PfamPF01770. Folate_carrier. 1 hit.
[Graphical view]
PIRSFPIRSF028739. Folate_carrier. 1 hit.
PIRSF500793. Folate_transporter_1. 1 hit.
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00806. rfc. 1 hit.
ProtoNetSearch...

Other

NextBio298695.
PROP41438.
SOURCESearch...

Entry information

Entry nameS19A1_MOUSE
AccessionPrimary (citable) accession number: P41438
Secondary accession number(s): Q62450, Q62451
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot