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P41411

- CDC18_SCHPO

UniProt

P41411 - CDC18_SCHPO

Protein

Cell division control protein 18

Gene

cdc18

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Part of the checkpoint control that prevents mitosis from occurring until S phase is completed. Plays a key role in coupling S phase to start and mitosis. Acts at the initiation of DNA replication and plays a major role in controlling the onset of S-phase. Together with orp1, involved in the maintenance of replication forks and activation of cds1-dependent S-phase checkpoint.5 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi199 – 2068ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA clamp loader activity Source: PomBase
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. DNA replication initiation involved in nuclear cell cycle DNA replication Source: PomBase
    3. mitotic DNA replication checkpoint Source: PomBase
    4. mitotic nuclear division Source: UniProtKB-KW
    5. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: PomBase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA replication, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188629. G1/S-Specific Transcription.
    REACT_209260. CDC6 association with the ORC:origin complex.
    REACT_209856. Association of licensing factors with the pre-replicative complex.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222044. Activation of the pre-replicative complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 18
    Gene namesi
    Name:cdc18
    ORF Names:SPBC14C8.07c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC14C8.07c.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear pre-replicative complex Source: PomBase
    2. nuclear replication fork Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 577577Cell division control protein 18PRO_0000150978Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by pop1 and pop2 and targeted to the 26S proteasome for degradation.

    Keywords - PTMi

    Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with the origin recognition complex (ORC) and is recruited to the ars1 origin of replication. Interacts with cdt1, pop1 and pop2.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pop1P870603EBI-1207853,EBI-1185389
    pop2O141703EBI-1207853,EBI-1185414

    Protein-protein interaction databases

    BioGridi276557. 51 interactions.
    IntActiP41411. 3 interactions.
    STRINGi4896.SPBC14C8.07c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP41411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CDC6/cdc18 family.Curated

    Phylogenomic databases

    eggNOGiCOG1474.
    KOiK02213.
    OMAiPRELNFH.
    OrthoDBiEOG7ZWD9N.
    PhylomeDBiP41411.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR015163. Cdc6_C_dom.
    IPR016314. Cell_div_Cdc6/18.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF09079. Cdc6_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001767. Cdc6. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    SM01074. Cdc6_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P41411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCETPIGCHT PRRCNRFIDS AALIDCTNKT NQREHSPSFS IEIPTTPSRK    50
    RTLASSHFQT PTKRIKYELG ELQEEKTDLY PNFPAQLKEN KKPKLPTTPQ 100
    TPKTPKRTIQ IVTPKSLNRT CNPVPFATRL LQSTPHRQLF PPTPSTPSTP 150
    SYNSTAKLSL RKSYRSAGVV GRENEKSIVE SFFRQHLDAN AGGALYVSGA 200
    PGTGKTVLLH NVLDHVVSDY PKVNVCYINC MTINEPKAIF EKIHSKIVKE 250
    EILENEDHHI NFQCELESHF TQSANELYNP VIIVLDEMDH LIAREQQVLY 300
    TLFEWPSRPT SRLILVGIAN ALDMTDRFLP RLRTKHITPK LLSFTPYTAQ 350
    EISTIIKARL KTAATTSEKN NPFTPIKSIS EVSDDSINVV SQHADETPFI 400
    HPAAIELCAR KVAASSGDLR KALDICRHAI ELAEREWKAQ HDNTLSSVDI 450
    PRASIAHVVR ATSAMSQSAS ARLKNLGLQQ KAILCTLVVC EKTSLSVADV 500
    FEKYSSLCLR DRLIYPLTSS EFCDVANSLE TLAIIRLRTK QRNGKPQDRI 550
    ISLLVPEMDV ITAVGDIGTL KRFFDRR 577
    Length:577
    Mass (Da):64,753
    Last modified:November 1, 1995 - v1
    Checksum:i74DBE9D5CF23504D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16793 Genomic DNA. Translation: AAA02871.1.
    CU329671 Genomic DNA. Translation: CAA18425.1.
    PIRiA40726.
    RefSeqiNP_595910.1. NM_001021818.2.

    Genome annotation databases

    EnsemblFungiiSPBC14C8.07c.1; SPBC14C8.07c.1:pep; SPBC14C8.07c.
    GeneIDi2540013.
    KEGGispo:SPBC14C8.07c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16793 Genomic DNA. Translation: AAA02871.1 .
    CU329671 Genomic DNA. Translation: CAA18425.1 .
    PIRi A40726.
    RefSeqi NP_595910.1. NM_001021818.2.

    3D structure databases

    ProteinModelPortali P41411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276557. 51 interactions.
    IntActi P41411. 3 interactions.
    STRINGi 4896.SPBC14C8.07c-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC14C8.07c.1 ; SPBC14C8.07c.1:pep ; SPBC14C8.07c .
    GeneIDi 2540013.
    KEGGi spo:SPBC14C8.07c.

    Organism-specific databases

    PomBasei SPBC14C8.07c.

    Phylogenomic databases

    eggNOGi COG1474.
    KOi K02213.
    OMAi PRELNFH.
    OrthoDBi EOG7ZWD9N.
    PhylomeDBi P41411.

    Enzyme and pathway databases

    Reactomei REACT_188629. G1/S-Specific Transcription.
    REACT_209260. CDC6 association with the ORC:origin complex.
    REACT_209856. Association of licensing factors with the pre-replicative complex.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_222044. Activation of the pre-replicative complex.

    Miscellaneous databases

    NextBioi 20801156.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR015163. Cdc6_C_dom.
    IPR016314. Cell_div_Cdc6/18.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF09079. Cdc6_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001767. Cdc6. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    SM01074. Cdc6_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The fission yeast cdc18+ gene product couples S phase to START and mitosis."
      Kelly T.J., Martin G.S., Forsburg S.L., Stephen R.J., Russo A., Nurse P.
      Cell 74:371-382(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast."
      Nishitani H., Nurse P.
      Cell 83:397-405(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18."
      Kominami K., Toda T.
      Genes Dev. 11:1548-1560(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH POP1.
    5. "sud1+ targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast."
      Jallepalli P.V., Tien D., Kelly T.J.
      Proc. Natl. Acad. Sci. U.S.A. 95:8159-8164(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POP2.
    6. "F-box/WD-repeat proteins pop1p and Sud1p/Pop2p form complexes that bind and direct the proteolysis of cdc18p."
      Wolf D.A., McKeon F., Jackson P.K.
      Curr. Biol. 9:373-376(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POP1 AND POP2.
    7. "The Cdt1 protein is required to license DNA for replication in fission yeast."
      Nishitani H., Lygerou Z., Nishimoto T., Nurse P.
      Nature 404:625-628(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDT1.
    8. "Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein."
      Chuang R.-Y., Chretien L., Dai J., Kelly T.J.
      J. Biol. Chem. 277:16920-16927(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ORC.
    9. "Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p."
      Murakami H., Yanow S.K., Griffiths D., Nakanishi M., Nurse P.
      Nat. Cell Biol. 4:384-388(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCDC18_SCHPO
    AccessioniPrimary (citable) accession number: P41411
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3