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Protein

Cell division control protein 18

Gene

cdc18

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the checkpoint control that prevents mitosis from occurring until S phase is completed. Plays a key role in coupling S phase to start and mitosis. Acts at the initiation of DNA replication and plays a major role in controlling the onset of S-phase. Together with orp1, involved in the maintenance of replication forks and activation of cds1-dependent S-phase checkpoint.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi199 – 2068ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA clamp loader activity Source: PomBase

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic DNA replication checkpoint Source: PomBase
  3. mitotic nuclear division Source: UniProtKB-KW
  4. mitotic pre-replicative complex assembly Source: PomBase
  5. positive regulation of DNA replication initiation Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA replication, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 18
Gene namesi
Name:cdc18
ORF Names:SPBC14C8.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

PomBaseiSPBC14C8.07c.

Subcellular locationi

GO - Cellular componenti

  1. nuclear chromatin Source: PomBase
  2. nuclear pre-replicative complex Source: PomBase
  3. nuclear replication fork Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Cell division control protein 18PRO_0000150978Add
BLAST

Post-translational modificationi

Ubiquitinated by pop1 and pop2 and targeted to the 26S proteasome for degradation.

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Interacts with the origin recognition complex (ORC) and is recruited to the ars1 origin of replication. Interacts with cdt1, pop1 and pop2.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pop1P870603EBI-1207853,EBI-1185389
pop2O141703EBI-1207853,EBI-1185414

Protein-protein interaction databases

BioGridi276557. 50 interactions.
IntActiP41411. 3 interactions.
STRINGi4896.SPBC14C8.07c-1.

Structurei

3D structure databases

ProteinModelPortaliP41411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC6/cdc18 family.Curated

Phylogenomic databases

eggNOGiCOG1474.
InParanoidiP41411.
KOiK02213.
OMAiPRELNFH.
OrthoDBiEOG7ZWD9N.
PhylomeDBiP41411.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR015163. Cdc6_C_dom.
IPR016314. Cell_div_Cdc6/18.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001767. Cdc6. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P41411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCETPIGCHT PRRCNRFIDS AALIDCTNKT NQREHSPSFS IEIPTTPSRK
60 70 80 90 100
RTLASSHFQT PTKRIKYELG ELQEEKTDLY PNFPAQLKEN KKPKLPTTPQ
110 120 130 140 150
TPKTPKRTIQ IVTPKSLNRT CNPVPFATRL LQSTPHRQLF PPTPSTPSTP
160 170 180 190 200
SYNSTAKLSL RKSYRSAGVV GRENEKSIVE SFFRQHLDAN AGGALYVSGA
210 220 230 240 250
PGTGKTVLLH NVLDHVVSDY PKVNVCYINC MTINEPKAIF EKIHSKIVKE
260 270 280 290 300
EILENEDHHI NFQCELESHF TQSANELYNP VIIVLDEMDH LIAREQQVLY
310 320 330 340 350
TLFEWPSRPT SRLILVGIAN ALDMTDRFLP RLRTKHITPK LLSFTPYTAQ
360 370 380 390 400
EISTIIKARL KTAATTSEKN NPFTPIKSIS EVSDDSINVV SQHADETPFI
410 420 430 440 450
HPAAIELCAR KVAASSGDLR KALDICRHAI ELAEREWKAQ HDNTLSSVDI
460 470 480 490 500
PRASIAHVVR ATSAMSQSAS ARLKNLGLQQ KAILCTLVVC EKTSLSVADV
510 520 530 540 550
FEKYSSLCLR DRLIYPLTSS EFCDVANSLE TLAIIRLRTK QRNGKPQDRI
560 570
ISLLVPEMDV ITAVGDIGTL KRFFDRR
Length:577
Mass (Da):64,753
Last modified:November 1, 1995 - v1
Checksum:i74DBE9D5CF23504D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16793 Genomic DNA. Translation: AAA02871.1.
CU329671 Genomic DNA. Translation: CAA18425.1.
PIRiA40726.
RefSeqiNP_595910.1. NM_001021818.2.

Genome annotation databases

EnsemblFungiiSPBC14C8.07c.1; SPBC14C8.07c.1:pep; SPBC14C8.07c.
GeneIDi2540013.
KEGGispo:SPBC14C8.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16793 Genomic DNA. Translation: AAA02871.1.
CU329671 Genomic DNA. Translation: CAA18425.1.
PIRiA40726.
RefSeqiNP_595910.1. NM_001021818.2.

3D structure databases

ProteinModelPortaliP41411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276557. 50 interactions.
IntActiP41411. 3 interactions.
STRINGi4896.SPBC14C8.07c-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14C8.07c.1; SPBC14C8.07c.1:pep; SPBC14C8.07c.
GeneIDi2540013.
KEGGispo:SPBC14C8.07c.

Organism-specific databases

PomBaseiSPBC14C8.07c.

Phylogenomic databases

eggNOGiCOG1474.
InParanoidiP41411.
KOiK02213.
OMAiPRELNFH.
OrthoDBiEOG7ZWD9N.
PhylomeDBiP41411.

Miscellaneous databases

NextBioi20801156.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR015163. Cdc6_C_dom.
IPR016314. Cell_div_Cdc6/18.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09079. Cdc6_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001767. Cdc6. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM01074. Cdc6_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The fission yeast cdc18+ gene product couples S phase to START and mitosis."
    Kelly T.J., Martin G.S., Forsburg S.L., Stephen R.J., Russo A., Nurse P.
    Cell 74:371-382(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast."
    Nishitani H., Nurse P.
    Cell 83:397-405(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18."
    Kominami K., Toda T.
    Genes Dev. 11:1548-1560(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POP1.
  5. "sud1+ targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast."
    Jallepalli P.V., Tien D., Kelly T.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:8159-8164(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POP2.
  6. "F-box/WD-repeat proteins pop1p and Sud1p/Pop2p form complexes that bind and direct the proteolysis of cdc18p."
    Wolf D.A., McKeon F., Jackson P.K.
    Curr. Biol. 9:373-376(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POP1 AND POP2.
  7. "The Cdt1 protein is required to license DNA for replication in fission yeast."
    Nishitani H., Lygerou Z., Nishimoto T., Nurse P.
    Nature 404:625-628(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDT1.
  8. "Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein."
    Chuang R.-Y., Chretien L., Dai J., Kelly T.J.
    J. Biol. Chem. 277:16920-16927(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ORC.
  9. "Maintenance of replication forks and the S-phase checkpoint by Cdc18p and Orp1p."
    Murakami H., Yanow S.K., Griffiths D., Nakanishi M., Nurse P.
    Nat. Cell Biol. 4:384-388(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCDC18_SCHPO
AccessioniPrimary (citable) accession number: P41411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.