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Protein

Pyrimidine-specific ribonucleoside hydrolase RihA

Gene

rihA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes with equal efficiency cytidine or uridine to ribose and cytosine or uracil, respectively.

Enzyme regulationi

Subject to catabolite repression.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1

GO - Molecular functioni

  • calcium ion binding Source: EcoliWiki
  • purine nucleosidase activity Source: GO_Central
  • pyrimidine-5'-nucleotide nucleosidase activity Source: EcoliWiki
  • uridine nucleosidase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6358-MONOMER.
ECOL316407:JW0646-MONOMER.
MetaCyc:G6358-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine-specific ribonucleoside hydrolase RihA (EC:3.2.-.-)
Alternative name(s):
Cytidine/uridine-specific hydrolase
Gene namesi
Name:rihA
Synonyms:ybeK
Ordered Locus Names:b0651, JW0646
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12701. rihA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002068131 – 311Pyrimidine-specific ribonucleoside hydrolase RihAAdd BLAST311

Proteomic databases

PaxDbiP41409.
PRIDEiP41409.

Interactioni

Protein-protein interaction databases

BioGridi4261645. 12 interactors.
IntActiP41409. 15 interactors.
STRINGi511145.b0651.

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi13 – 23Combined sources11
Beta strandi28 – 35Combined sources8
Beta strandi37 – 40Combined sources4
Helixi42 – 55Combined sources14
Beta strandi63 – 65Combined sources3
Beta strandi71 – 73Combined sources3
Turni79 – 81Combined sources3
Turni83 – 85Combined sources3
Helixi104 – 114Combined sources11
Beta strandi119 – 123Combined sources5
Helixi128 – 136Combined sources9
Helixi138 – 143Combined sources6
Beta strandi144 – 150Combined sources7
Beta strandi158 – 162Combined sources5
Helixi165 – 169Combined sources5
Helixi171 – 178Combined sources8
Beta strandi180 – 182Combined sources3
Beta strandi184 – 187Combined sources4
Helixi189 – 192Combined sources4
Beta strandi195 – 197Combined sources3
Helixi199 – 208Combined sources10
Helixi211 – 224Combined sources14
Beta strandi235 – 238Combined sources4
Helixi242 – 249Combined sources8
Helixi251 – 253Combined sources3
Beta strandi254 – 259Combined sources6
Beta strandi261 – 263Combined sources3
Beta strandi274 – 276Combined sources3
Beta strandi288 – 294Combined sources7
Helixi296 – 307Combined sources12
Helixi308 – 310Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YOEX-ray1.78A2-311[»]
3G5IX-ray2.10A/B/C/D2-311[»]
ProteinModelPortaliP41409.
SMRiP41409.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41409.

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family. RihA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105DU0. Bacteria.
COG1957. LUCA.
HOGENOMiHOG000218839.
InParanoidiP41409.
KOiK01250.
OMAiFLGRHIN.
PhylomeDBiP41409.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
HAMAPiMF_01431. Pyrim_hydro_RihA. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
IPR022975. Pyrim_hydro_RihA.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPILLDCD PGHDDAIAIV LALASPELDV KAITSSAGNQ TPEKTLRNVL
60 70 80 90 100
RMLTLLNRTD IPVAGGAVKP LMRELIIADN VHGESGLDGP ALPEPTFAPQ
110 120 130 140 150
NCTAVELMAK TLRESAEPVT IVSTGPQTNV ALLLNSHPEL HSKIARIVIM
160 170 180 190 200
GGAMGLGNWT PAAEFNIYVD PEAAEIVFQS GIPVVMAGLD VTHKAQIHVE
210 220 230 240 250
DTERFRAIGN PVSTIVAELL DFFLEYHKDE KWGFVGAPLH DPCTIAWLLK
260 270 280 290 300
PELFTSVERW VGVETQGKYT QGMTVVDYYY LTGNKPNATV MVDVDRQGFV
310
DLLADRLKFY A
Length:311
Mass (Da):33,823
Last modified:November 1, 1997 - v2
Checksum:iEEB6CF8C60BABDD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40852.1.
U00096 Genomic DNA. Translation: AAC73752.1.
AP009048 Genomic DNA. Translation: BAA35303.1.
U10981 Genomic DNA. No translation available.
PIRiA64800.
RefSeqiNP_415184.1. NC_000913.3.
WP_001207520.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73752; AAC73752; b0651.
BAA35303; BAA35303; BAA35303.
GeneIDi945503.
KEGGiecj:JW0646.
eco:b0651.
PATRICi32116485. VBIEscCol129921_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40852.1.
U00096 Genomic DNA. Translation: AAC73752.1.
AP009048 Genomic DNA. Translation: BAA35303.1.
U10981 Genomic DNA. No translation available.
PIRiA64800.
RefSeqiNP_415184.1. NC_000913.3.
WP_001207520.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YOEX-ray1.78A2-311[»]
3G5IX-ray2.10A/B/C/D2-311[»]
ProteinModelPortaliP41409.
SMRiP41409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261645. 12 interactors.
IntActiP41409. 15 interactors.
STRINGi511145.b0651.

Proteomic databases

PaxDbiP41409.
PRIDEiP41409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73752; AAC73752; b0651.
BAA35303; BAA35303; BAA35303.
GeneIDi945503.
KEGGiecj:JW0646.
eco:b0651.
PATRICi32116485. VBIEscCol129921_0682.

Organism-specific databases

EchoBASEiEB2563.
EcoGeneiEG12701. rihA.

Phylogenomic databases

eggNOGiENOG4105DU0. Bacteria.
COG1957. LUCA.
HOGENOMiHOG000218839.
InParanoidiP41409.
KOiK01250.
OMAiFLGRHIN.
PhylomeDBiP41409.

Enzyme and pathway databases

BioCyciEcoCyc:G6358-MONOMER.
ECOL316407:JW0646-MONOMER.
MetaCyc:G6358-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP41409.
PROiP41409.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
HAMAPiMF_01431. Pyrim_hydro_RihA. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
IPR022975. Pyrim_hydro_RihA.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIHA_ECOLI
AccessioniPrimary (citable) accession number: P41409
Secondary accession number(s): P77738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Strictly specific for ribonucleosides.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.