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P41409 (RIHA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyrimidine-specific ribonucleoside hydrolase RihA
EC=3.2.-.-
Alternative name(s):
Cytidine/uridine-specific hydrolase
Gene names
Name:rihA
Synonyms:ybeK
Ordered Locus Names:b0651, JW0646
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes with equal efficiency cytidine or uridine to ribose and cytosine or uracil, respectively. HAMAP-Rule MF_01431

Enzyme regulation

Subject to catabolite repression. HAMAP-Rule MF_01431

Miscellaneous

Strictly specific for ribonucleosides. HAMAP-Rule MF_01431

Sequence similarities

Belongs to the IUNH family. RihA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Pyrimidine-specific ribonucleoside hydrolase RihA HAMAP-Rule MF_01431
PRO_0000206813

Sites

Active site2401 By similarity

Secondary structure

1 .............................................................. 311
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P41409 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: EEB6CF8C60BABDD4

FASTA31133,823
        10         20         30         40         50         60 
MALPILLDCD PGHDDAIAIV LALASPELDV KAITSSAGNQ TPEKTLRNVL RMLTLLNRTD 

        70         80         90        100        110        120 
IPVAGGAVKP LMRELIIADN VHGESGLDGP ALPEPTFAPQ NCTAVELMAK TLRESAEPVT 

       130        140        150        160        170        180 
IVSTGPQTNV ALLLNSHPEL HSKIARIVIM GGAMGLGNWT PAAEFNIYVD PEAAEIVFQS 

       190        200        210        220        230        240 
GIPVVMAGLD VTHKAQIHVE DTERFRAIGN PVSTIVAELL DFFLEYHKDE KWGFVGAPLH 

       250        260        270        280        290        300 
DPCTIAWLLK PELFTSVERW VGVETQGKYT QGMTVVDYYY LTGNKPNATV MVDVDRQGFV 

       310 
DLLADRLKFY A

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]Lum D., Wallace B.J.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-123.
Strain: K12 / BK9MDG.
[6]"The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation."
Petersen C., Moeller L.B.
J. Biol. Chem. 276:884-894(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U82598 Genomic DNA. Translation: AAB40852.1.
U00096 Genomic DNA. Translation: AAC73752.1.
AP009048 Genomic DNA. Translation: BAA35303.1.
U10981 Genomic DNA. No translation available.
PIRA64800.
RefSeqNP_415184.1. NC_000913.2.
YP_488942.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YOEX-ray1.78A2-311[»]
3G5IX-ray2.10A/B/C/D2-311[»]
ProteinModelPortalP41409.
SMRP41409. Positions 2-311.
ModBaseSearch...

Protein-protein interaction databases

IntActP41409. 15 interactions.
STRING511145.b0651.

Proteomic databases

PaxDbP41409.
PRIDEP41409.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73752; AAC73752; b0651.
BAA35303; BAA35303; BAA35303.
GeneID12932651.
945503.
KEGGecj:Y75_p0641.
eco:b0651.
PATRIC32116485. VBIEscCol129921_0682.

Organism-specific databases

EchoBASEEB2563.
EcoGeneEG12701. rihA.

Phylogenomic databases

eggNOGCOG1957.
HOGENOMHOG000218839.
KOK01250.
OMAYLIRTLR.
ProtClustDBPRK10443.

Enzyme and pathway databases

BioCycEcoCyc:G6358-MONOMER.
ECOL316407:JW0646-MONOMER.
MetaCyc:G6358-MONOMER.

Gene expression databases

GenevestigatorP41409.

Family and domain databases

Gene3D3.90.245.10. 1 hit.
HAMAPMF_01431. Pyrim_hydro_RihA.
InterProIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
IPR022975. Pyrim_hydro_RihA.
[Graphical view]
PANTHERPTHR12304. PTHR12304. 1 hit.
PfamPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMSSF53590. I/U_nuclsd_hydro. 1 hit.
PROSITEPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP41409.

Entry information

Entry nameRIHA_ECOLI
AccessionPrimary (citable) accession number: P41409
Secondary accession number(s): P77738
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents