Reviewed, UniProtKB/Swiss-Prot P41403 (AK_MYCSM)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase
      Short name=ASK

Gene names

Name:

ask

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Feedback inhibition by lysine and threonine By similarity.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P41403-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P41403-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Aspartokinase		PRO_0000002383
Regions
	Domain	266 – 341	76	ACT 1
	Domain	348 – 413	66	ACT 2
Natural variations
	Alternative sequence	1 – 249	249	Missing in isoform Beta.		VSP_018662

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 926B9FC8625E20CD	FASTA	421	44,458
	10 20 30 40 50 60 MALVVQKYGG SSVADAERIR RVAERIVETK KAGNDVVVVV SAMGDTTDDL LDLARQVSPA 70 80 90 100 110 120 PPPREMDMLL TAGERISNAL VAMAIESLGA QARSFTGSQA GVITTGTHGN AKIIDVTPGR 130 140 150 160 170 180 LRDALDEGQI VLVAGFQGVS QDSKDVTTLG RGGSDTTAVA VAAALDADVC EIYTDVDGIF 190 200 210 220 230 240 TADPRIVPNA RHLDTVSFEE MLEMAACGAK VLMLRCVEYA RRYNVPIHVR SSYSDKPGTI 250 260 270 280 290 300 VKGSIEDIPM EDAILTGVAH DRSEAKVTVV GLPDVPGYAA KVFRAVAEAD VNIDMVLQNI 310 320 330 340 350 360 SKIEDGKTDI TFTCARDNGP RAVEKLSALK SEIGFSQVLY DDHIGKVSLI GAGMRSHPGV 370 380 390 400 410 420 TATFCEALAE AGINIDLIST SEIRISVLIK DTELDKAVSA LHEAFGLGGD DEAVVYAGTG R
	Isoform Beta (Aspartokinase subunit beta) [UniParc]. Checksum: 97B198209C02FF5C		172	18,131
	10 20 30 40 50 60 MEDAILTGVA HDRSEAKVTV VGLPDVPGYA AKVFRAVAEA DVNIDMVLQN ISKIEDGKTD 70 80 90 100 110 120 ITFTCARDNG PRAVEKLSAL KSEIGFSQVL YDDHIGKVSL IGAGMRSHPG VTATFCEALA 130 140 150 160 170 EAGINIDLIS TSEIRISVLI KDTELDKAVS ALHEAFGLGG DDEAVVYAGT GR

References

[1]

"Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria." Cirillo J.D., Weisbrod T.R., Pascopella L., Bloom B.R., Jacobs W.R. Jr. Mol. Microbiol. 11:629-639(1994) [PubMed: 7910936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 607 / DSM 43465 / mc(2)6 / NCTC 7017 / NRRL B-692.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z17372 Genomic DNA. Translation: CAA78984.1. Z17372 Genomic DNA. Translation: CAA78985.1. Different initiation.
PIR	S42422.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kin_reg. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_MYCSM

Accession

Primary (citable) accession number: P41403

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)