Reviewed, UniProtKB/Swiss-Prot P41398 (AK_CORFL)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Name:	lysC
Synonyms:	ask

Organism

Corynebacterium flavum

Taxonomic identifier

28028 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Low feedback inhibition by lysine and threonine. The decreased sensitivity of C.flavum, as compared to C.glutamicum, is probably due to the Asp-345-Gly sequence difference.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P41398-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P41398-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.
     250-250: V → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Aspartokinase		PRO_0000002377
Regions
	Domain	266 – 339	74	ACT 1
	Domain	348 – 413	66	ACT 2
Natural variations
	Alternative sequence	1 – 249	249	Missing in isoform Beta.		VSP_018657
	Alternative sequence	250	1	V → M in isoform Beta.		VSP_018658
Experimental info
	Mutagenesis	345	1	D → G: Decreased sensitivity of AK activity to concerted feedback inhibition by lysine and threonine.

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4B37B0B6597F665E	FASTA	421	44,797
	10 20 30 40 50 60 MALVVQKYGG SSLESAERIR NVAERIVATK KAGNDVVVVC SAMGDTTDEL LELAAAVNPV 70 80 90 100 110 120 PPAREMDMLL TAGERISNAL VAMAIESLGA EAQSFTGSQA GVLTTERHGN ARIVDVTPGR 130 140 150 160 170 180 VREALDEGKI CIVAGFQGVN KETRDVTTLG RGGSDTTAVA LAAALNADVC EIYSDVDGVY 190 200 210 220 230 240 TADPRIVPNA QKLEKLSFEE MLELAAVGSK ILVLRSVEYA RAFNVPLRVR SSYSNDPGTL 250 260 270 280 290 300 IAGSMEDIPV EEAVLTGVAT DKSEAKVTVL GISDKPGEAA KVFRALADAE INIDMVLQNV 310 320 330 340 350 360 SSVEDGTTDI TFTCPRADGR RAMEILKKLQ VQGNWTNVLY DDQVDKVSLV GAGMKSHPGV 370 380 390 400 410 420 TAEFMEALRD VNVNIELIST SEIRISVLIR EDDLDAAARA LHEQFQLGGE DEAVVYAGTG R
	Isoform Beta (Aspartokinase subunit beta). Checksum: BC5043A4B488F0AF	FASTA	172	18,579
	10 20 30 40 50 60 MEEAVLTGVA TDKSEAKVTV LGISDKPGEA AKVFRALADA EINIDMVLQN VSSVEDGTTD 70 80 90 100 110 120 ITFTCPRADG RRAMEILKKL QVQGNWTNVL YDDQVDKVSL VGAGMKSHPG VTAEFMEALR 130 140 150 160 170 DVNVNIELIS TSEIRISVLI REDDLDAAAR ALHEQFQLGG EDEAVVYAGT GR

References

[1]	"Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon." Follettie M.T., Peoples O.P., Agoropoulou C., Sinskey A.J. J. Bacteriol. 175:4096-4103(1993) [PubMed: 8100567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: N13.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L16848 Genomic DNA. Translation: AAA23293.1.
3D structure databases
SMR	P41398. Positions 253-416.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.4. 270601.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_CORFL

Accession

Primary (citable) accession number: P41398

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)