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Protein

Amidophosphoribosyltransferase

Gene

ade4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ade4)
  2. Bifunctional purine biosynthetic protein ADE1 (ade1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Metal bindingi383 – 3831MagnesiumBy similarity
Metal bindingi384 – 3841MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_341437. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ade4
ORF Names:SPAC4D7.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.08c.
PomBaseiSPAC4D7.08c. ade4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 533532AmidophosphoribosyltransferasePRO_0000139646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei506 – 5061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41390.
PaxDbiP41390.

Interactioni

Protein-protein interaction databases

BioGridi280040. 3 interactions.
MINTiMINT-4690052.
STRINGi4896.SPAC4D7.08c.1.

Structurei

3D structure databases

ProteinModelPortaliP41390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 238237Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
InParanoidiP41390.
KOiK00764.
OMAiAARVHMG.
OrthoDBiEOG74R20H.
PhylomeDBiP41390.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILALMLA DPHQQACPEI YEGLYSLQHR GQDAAGIVTA GNKGRLYQCK
60 70 80 90 100
GSGMVADVFS QHQLRQLVGS MGIGHLRYPT AGSCAHSEAQ PFYVNSPYGL
110 120 130 140 150
VLGHNGNLIN GPELRRFLDT EAHRHVNTGS DSELLLNIFA YELQRLDKFR
160 170 180 190 200
INENDIFEAL RNVYDRVNGG YACVAMIAGL GVLGFRDPNG IRPLVIGERD
210 220 230 240 250
TPEGKDYMLA SESVVLTQFG YRTFRDIRPG ECVFIRRSNR EDILAGFRGP
260 270 280 290 300
RLFSRQILPC LRFTPDIFEY VYFARPDSVI DGLSVYQSRL NMGEKLAHTI
310 320 330 340 350
MKRFGPDYME KIDAVIPVPD SARTSALALA QTAQLPYVEA FIKNRYIGRT
360 370 380 390 400
FIMPGQQIRR KSVRRKLNVQ PQEFFDKNVL IVDDSIVRGT TSREIVQMAR
410 420 430 440 450
ESGAKNVYLA SCAPMITHPH VYGIDLADCK DLIAYGKTED EVAEAISADG
460 470 480 490 500
VIYQTLEDLL DSCRTAELTE FEVGLFTGEY TTGASKEYLV HLEQMRIANN
510 520 530
RARKHSFAED EEREAPEDIS LHNTHSDVTF DFV
Length:533
Mass (Da):59,832
Last modified:November 1, 1995 - v1
Checksum:i53C7C99F30A68B57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72293 Genomic DNA. Translation: CAA51034.1.
CU329670 Genomic DNA. Translation: CAB11280.1.
PIRiS43526.
RefSeqiNP_594961.1. NM_001020392.2.

Genome annotation databases

EnsemblFungiiSPAC4D7.08c.1; SPAC4D7.08c.1:pep; SPAC4D7.08c.
GeneIDi2543626.
KEGGispo:SPAC4D7.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72293 Genomic DNA. Translation: CAA51034.1.
CU329670 Genomic DNA. Translation: CAB11280.1.
PIRiS43526.
RefSeqiNP_594961.1. NM_001020392.2.

3D structure databases

ProteinModelPortaliP41390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280040. 3 interactions.
MINTiMINT-4690052.
STRINGi4896.SPAC4D7.08c.1.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

MaxQBiP41390.
PaxDbiP41390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4D7.08c.1; SPAC4D7.08c.1:pep; SPAC4D7.08c.
GeneIDi2543626.
KEGGispo:SPAC4D7.08c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.08c.
PomBaseiSPAC4D7.08c. ade4.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
InParanoidiP41390.
KOiK00764.
OMAiAARVHMG.
OrthoDBiEOG74R20H.
PhylomeDBiP41390.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
ReactomeiREACT_341437. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi20804632.
PROiP41390.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ade4 gene of Schizosaccharomyces pombe: cloning, sequence and regulation."
    Ludin K.M., Hilti N., Schweingruber M.E.
    Curr. Genet. 25:465-468(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPUR1_SCHPO
AccessioniPrimary (citable) accession number: P41390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.