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P41390

- PUR1_SCHPO

UniProt

P41390 - PUR1_SCHPO

Protein

Amidophosphoribosyltransferase

Gene

ade4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Metal bindingi383 – 3831MagnesiumBy similarity
    Metal bindingi384 – 3841MagnesiumBy similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: PomBase
    2. adenine metabolic process Source: PomBase
    3. glutamine metabolic process Source: UniProtKB-KW
    4. nucleoside metabolic process Source: InterPro
    5. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_207552. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Gene namesi
    Name:ade4
    ORF Names:SPAC4D7.08c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC4D7.08c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 533532AmidophosphoribosyltransferasePRO_0000139646Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei506 – 5061Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP41390.
    PaxDbiP41390.

    Interactioni

    Protein-protein interaction databases

    BioGridi280040. 3 interactions.
    MINTiMINT-4690052.
    STRINGi4896.SPAC4D7.08c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP41390.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 238237Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033687.
    KOiK00764.
    OMAiPITHAHI.
    OrthoDBiEOG74R20H.
    PhylomeDBiP41390.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41390-1 [UniParc]FASTAAdd to Basket

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    MCGILALMLA DPHQQACPEI YEGLYSLQHR GQDAAGIVTA GNKGRLYQCK    50
    GSGMVADVFS QHQLRQLVGS MGIGHLRYPT AGSCAHSEAQ PFYVNSPYGL 100
    VLGHNGNLIN GPELRRFLDT EAHRHVNTGS DSELLLNIFA YELQRLDKFR 150
    INENDIFEAL RNVYDRVNGG YACVAMIAGL GVLGFRDPNG IRPLVIGERD 200
    TPEGKDYMLA SESVVLTQFG YRTFRDIRPG ECVFIRRSNR EDILAGFRGP 250
    RLFSRQILPC LRFTPDIFEY VYFARPDSVI DGLSVYQSRL NMGEKLAHTI 300
    MKRFGPDYME KIDAVIPVPD SARTSALALA QTAQLPYVEA FIKNRYIGRT 350
    FIMPGQQIRR KSVRRKLNVQ PQEFFDKNVL IVDDSIVRGT TSREIVQMAR 400
    ESGAKNVYLA SCAPMITHPH VYGIDLADCK DLIAYGKTED EVAEAISADG 450
    VIYQTLEDLL DSCRTAELTE FEVGLFTGEY TTGASKEYLV HLEQMRIANN 500
    RARKHSFAED EEREAPEDIS LHNTHSDVTF DFV 533
    Length:533
    Mass (Da):59,832
    Last modified:November 1, 1995 - v1
    Checksum:i53C7C99F30A68B57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72293 Genomic DNA. Translation: CAA51034.1.
    CU329670 Genomic DNA. Translation: CAB11280.1.
    PIRiS43526.
    RefSeqiNP_594961.1. NM_001020392.2.

    Genome annotation databases

    EnsemblFungiiSPAC4D7.08c.1; SPAC4D7.08c.1:pep; SPAC4D7.08c.
    GeneIDi2543626.
    KEGGispo:SPAC4D7.08c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72293 Genomic DNA. Translation: CAA51034.1 .
    CU329670 Genomic DNA. Translation: CAB11280.1 .
    PIRi S43526.
    RefSeqi NP_594961.1. NM_001020392.2.

    3D structure databases

    ProteinModelPortali P41390.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 280040. 3 interactions.
    MINTi MINT-4690052.
    STRINGi 4896.SPAC4D7.08c-1.

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    MaxQBi P41390.
    PaxDbi P41390.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC4D7.08c.1 ; SPAC4D7.08c.1:pep ; SPAC4D7.08c .
    GeneIDi 2543626.
    KEGGi spo:SPAC4D7.08c.

    Organism-specific databases

    PomBasei SPAC4D7.08c.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033687.
    KOi K00764.
    OMAi PITHAHI.
    OrthoDBi EOG74R20H.
    PhylomeDBi P41390.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    Reactomei REACT_207552. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    NextBioi 20804632.
    PROi P41390.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ade4 gene of Schizosaccharomyces pombe: cloning, sequence and regulation."
      Ludin K.M., Hilti N., Schweingruber M.E.
      Curr. Genet. 25:465-468(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPUR1_SCHPO
    AccessioniPrimary (citable) accession number: P41390
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3