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P41390

- PUR1_SCHPO

UniProt

P41390 - PUR1_SCHPO

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Protein

Amidophosphoribosyltransferase

Gene

ade4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg(2+) ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Metal bindingi383 – 3831MagnesiumBy similarity
Metal bindingi384 – 3841MagnesiumBy similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: PomBase
  2. adenine metabolic process Source: PomBase
  3. glutamine metabolic process Source: UniProtKB-KW
  4. nucleoside metabolic process Source: InterPro
  5. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_207552. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ade4
ORF Names:SPAC4D7.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC4D7.08c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 533532AmidophosphoribosyltransferasePRO_0000139646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei506 – 5061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41390.
PaxDbiP41390.

Interactioni

Protein-protein interaction databases

BioGridi280040. 3 interactions.
MINTiMINT-4690052.
STRINGi4896.SPAC4D7.08c-1.

Structurei

3D structure databases

ProteinModelPortaliP41390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 238237Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
InParanoidiP41390.
KOiK00764.
OMAiPITHAHI.
OrthoDBiEOG74R20H.
PhylomeDBiP41390.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41390-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILALMLA DPHQQACPEI YEGLYSLQHR GQDAAGIVTA GNKGRLYQCK
60 70 80 90 100
GSGMVADVFS QHQLRQLVGS MGIGHLRYPT AGSCAHSEAQ PFYVNSPYGL
110 120 130 140 150
VLGHNGNLIN GPELRRFLDT EAHRHVNTGS DSELLLNIFA YELQRLDKFR
160 170 180 190 200
INENDIFEAL RNVYDRVNGG YACVAMIAGL GVLGFRDPNG IRPLVIGERD
210 220 230 240 250
TPEGKDYMLA SESVVLTQFG YRTFRDIRPG ECVFIRRSNR EDILAGFRGP
260 270 280 290 300
RLFSRQILPC LRFTPDIFEY VYFARPDSVI DGLSVYQSRL NMGEKLAHTI
310 320 330 340 350
MKRFGPDYME KIDAVIPVPD SARTSALALA QTAQLPYVEA FIKNRYIGRT
360 370 380 390 400
FIMPGQQIRR KSVRRKLNVQ PQEFFDKNVL IVDDSIVRGT TSREIVQMAR
410 420 430 440 450
ESGAKNVYLA SCAPMITHPH VYGIDLADCK DLIAYGKTED EVAEAISADG
460 470 480 490 500
VIYQTLEDLL DSCRTAELTE FEVGLFTGEY TTGASKEYLV HLEQMRIANN
510 520 530
RARKHSFAED EEREAPEDIS LHNTHSDVTF DFV
Length:533
Mass (Da):59,832
Last modified:November 1, 1995 - v1
Checksum:i53C7C99F30A68B57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72293 Genomic DNA. Translation: CAA51034.1.
CU329670 Genomic DNA. Translation: CAB11280.1.
PIRiS43526.
RefSeqiNP_594961.1. NM_001020392.2.

Genome annotation databases

EnsemblFungiiSPAC4D7.08c.1; SPAC4D7.08c.1:pep; SPAC4D7.08c.
GeneIDi2543626.
KEGGispo:SPAC4D7.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72293 Genomic DNA. Translation: CAA51034.1 .
CU329670 Genomic DNA. Translation: CAB11280.1 .
PIRi S43526.
RefSeqi NP_594961.1. NM_001020392.2.

3D structure databases

ProteinModelPortali P41390.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 280040. 3 interactions.
MINTi MINT-4690052.
STRINGi 4896.SPAC4D7.08c-1.

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

MaxQBi P41390.
PaxDbi P41390.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC4D7.08c.1 ; SPAC4D7.08c.1:pep ; SPAC4D7.08c .
GeneIDi 2543626.
KEGGi spo:SPAC4D7.08c.

Organism-specific databases

PomBasei SPAC4D7.08c.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033687.
InParanoidi P41390.
KOi K00764.
OMAi PITHAHI.
OrthoDBi EOG74R20H.
PhylomeDBi P41390.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
Reactomei REACT_207552. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi 20804632.
PROi P41390.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ade4 gene of Schizosaccharomyces pombe: cloning, sequence and regulation."
    Ludin K.M., Hilti N., Schweingruber M.E.
    Curr. Genet. 25:465-468(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPUR1_SCHPO
AccessioniPrimary (citable) accession number: P41390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3