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Protein

DNA replication licensing factor mcm5

Gene

mcm5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Role in DNA replication and essential for viability.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi372 – 379ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • mitotic DNA replication initiation Source: PomBase
  • negative regulation of helicase activity Source: PomBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-68962 Activation of the pre-replicative complex
R-SPO-69052 Switching of origins to a post-replicative state
R-SPO-69300 Removal of licensing factors from origins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm5 (EC:3.6.4.12)
Alternative name(s):
Cell division control protein nda4
Minichromosome maintenance protein 5
Gene namesi
Name:mcm5
Synonyms:nda4
ORF Names:SPAC1B2.05, SPAC3F10.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC1B2.05 mcm5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941121 – 720DNA replication licensing factor mcm5Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350Phosphoserine1 Publication1
Modified residuei352Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41389
PaxDbiP41389
PRIDEiP41389

PTM databases

iPTMnetiP41389

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPAC1687.04O944502EBI-913821,EBI-7492115

Protein-protein interaction databases

IntActiP41389, 4 interactors
MINTiP41389
STRINGi4896.SPAC1B2.05.1

Structurei

3D structure databases

ProteinModelPortaliP41389
SMRiP41389
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini322 – 529MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi504 – 507Arginine finger4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi307 – 312Poly-Glu6

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

HOGENOMiHOG000224128
InParanoidiP41389
OrthoDBiEOG092C0VUM
PhylomeDBiP41389

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008048 MCM5
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01661 MCMPROTEIN5
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P41389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATGWERS AVYYTPVLPG EQELDSNVSH EKNFIQFIEE FVIDNDFIYR
60 70 80 90 100
TQLRDNLVVK QYMLNIDLRH LISYNEDLAH LLLSQPTDIL PLFESAVTTV
110 120 130 140 150
AKRLLYRSQE NASTNIPTCQ VTLRYDANIL PIRNLTASHI SKLVRVPGII
160 170 180 190 200
IGASTLSCRA TALHLVCRNC RATRILQISG GFSGVQLPRV CEAPVLDGEK
210 220 230 240 250
KDCPMDPFII DHSKSTFIDQ QVLKLQEAPD MVPVGELPRH ILLNADRYLT
260 270 280 290 300
NQITPGTRCV ITGIFSIFQN KSVKASGAVA IRNPYIRVVG IQMDSNDGSK
310 320 330 340 350
STPLFSEEEE EEFLEISRTP NLYDIISNSI SPAIYGNVDI KKAIACLLFS
360 370 380 390 400
GSKKILPDGM RLRGDINVLL LGDPGTAKSQ FLKFVERLAP IAVYTSGKGS
410 420 430 440 450
SAAGLTASIQ RDSVTREFYL EGGAMVLADG GIVCIDEFDK MRDEDRVAIH
460 470 480 490 500
EAMEQQTISI AKAGITTILN SRTSVLAAAN PIFGRYDDMK TPGENIDFQS
510 520 530 540 550
TILSRFDMIF IVKDEHDETK DRNIARHVIN LHTNLQESSE TLAIGEIPFD
560 570 580 590 600
KFRRYINYCR HKCAPNLDAE AAEKLSSQFV AIRKLVHQSE QDSNSRSTIP
610 620 630 640 650
ITVRQLEAII RITESLAKMS LSPIASEAHA TEAIRLFLTS TLAAATQSSP
660 670 680 690 700
EVTEEVKKIE ASLRKRLPIG FQASYRMLIR EYVNGHGYSQ HALEMALQIR
710 720
SSKETIQLRN GGQTVYRSGV
Length:720
Mass (Da):80,100
Last modified:October 1, 1996 - v2
Checksum:i52F3EA9DD127E56E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti461A → R in AAC60568 (PubMed:8298187).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68467 Genomic DNA Translation: AAC60568.1
CU329670 Genomic DNA Translation: CAB61472.1
PIRiA48723
T50141

Similar proteinsi

Entry informationi

Entry nameiMCM5_SCHPO
AccessioniPrimary (citable) accession number: P41389
Secondary accession number(s): Q9UTF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health