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P41368

- SYI2_STAAU

UniProt

P41368 - SYI2_STAAU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.By similarity
    Confers high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis.

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei588 – 5881ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Antibiotic resistance, Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ileS
    Synonyms:mupR
    Encoded oniPlasmid0 Publication
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10241024Isoleucine--tRNA ligasePRO_0000098562Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP41368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi585 – 5895"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41368-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKKYLNTQN EISAFWNTQK IFKKSIDNRK GQESFVFYDG PPTANGLPHA     50
    GHVLGRVIKD LVARLKTMQG FYVERKAGWD THGLPVELEV EKKIGIKGKQ 100
    DIEKYGIENF INECKKSVFN YEKEWRDFSK DLGYWVDMDS PYITLENNYI 150
    ESVWNILSTF HKKGLLYKGH KVTPYCTHDQ TALSSHEVAQ GYKNVKDLSA 200
    VVKFQLTNSK DTYFLSWTTT PWTLPANVAL AINKDLNYSK IRVENEYYIL 250
    ATDLINSIIT EKYEIIDTFS GSNLINLKYI PPFESDGLVN AYYVVDGEFV 300
    TNSEGTGIVH IAPAHGEDDY QLVLERDLDF LNVITREGVY NDRFPELVGN 350
    KAKNSDIEII KLLSKKQLLY KKQKYEHNYP HCWRCGNPLI YYAMEGWFIK 400
    TTNFKNEIIN NNNNIEWFPS HIKEGRMGNF LENMVDWNIG RNRYWGTPLN 450
    VWICNDCNHE YAPSSIKDLQ NNSINKIDED IELHRPYVDN ITLSCPKCNG 500
    KMSRVEEVID VWFDSGSMPF AQHHYPFDNQ KIFNQHFPAD FIAEGVDQTR 550
    GWFYSLLVIS TILKGKSSYK RALSLGHILD SNGKKMSKSK GNVINPTELI 600
    NKYGADSLRW ALISDSAPWN NKRFSENIVA QTKSKFIDTL DNIYKFYNMY 650
    NKIDHYNPNN EITKSRNTLD NWALSRLNTL IKESNIYVNN YDFTSAARLI 700
    NEYTNTISNW YIGDSRGRFW EQGISNDKKD AYNTLYEILT TLSRLVAPFV 750
    PFISEKIHYN LTGKSVHLQD YPQYKESFIN QALEDEMHTV IKIVELSRQA 800
    RKNADLKIKQ PLSKMVIKPN SQLNLSFLPN YYSIIKDELN IKNIELTDNI 850
    NDYITYELKL NFSSVGPKLG NKTKNIQTLI DSLSEYDKKS LIESNNFKSL 900
    SSDAELTKDD FIIKTLPKDS YQLSEDNDCV ILLDKNLSPE LIREGHAREL 950
    IRLIQQLRKK KNLPINQRID IYIGVTGELL ESIKTNKNMF KENFVIKNIH 1000
    LNVIDEYENT IHFNNKEIKI SLLY 1024
    Length:1,024
    Mass (Da):118,876
    Last modified:November 1, 1995 - v1
    Checksum:i56ECD232CA0C8430
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti519 – 5191P → R(PubMed:1903747)Curated
    Sequence conflicti591 – 5933GNV → ETF in CAA42080. (PubMed:1903747)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75439 Genomic DNA. Translation: CAA53189.1.
    X59478 Genomic DNA. Translation: CAA42080.1.
    X59477 Genomic DNA. Translation: CAA42079.1.
    RefSeqiWP_012263521.1. NC_010279.1.
    YP_001653102.1. NC_010279.1.

    Genome annotation databases

    GeneIDi5857592.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75439 Genomic DNA. Translation: CAA53189.1 .
    X59478 Genomic DNA. Translation: CAA42080.1 .
    X59477 Genomic DNA. Translation: CAA42079.1 .
    RefSeqi WP_012263521.1. NC_010279.1.
    YP_001653102.1. NC_010279.1.

    3D structure databases

    ProteinModelPortali P41368.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5857592.

    Phylogenomic databases

    eggNOGi COG0060.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870."
      Hodgson J.E., Curnock S.P., Dyke K.G.H., Morris R., Sylvester D.R., Gross M.S.
      Antimicrob. Agents Chemother. 38:1205-1208(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: J2870.
    2. "Cloning of the gene conferring resistance to mupirocin in Staphylococcus aureus."
      Dyke K.G.H., Curnock S.P., Golding M., Noble W.C.
      FEMS Microbiol. Lett. 61:195-198(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 504-519 AND 550-609.
      Strain: J2870.

    Entry informationi

    Entry nameiSYI2_STAAU
    AccessioniPrimary (citable) accession number: P41368
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3