Reviewed,
UniProtKB/Swiss-Prot P41368 (SYI2_STAAU)
Last modified
June 16, 2009.
Version 62.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Isoleucyl-tRNA synthetase EC=6.1.1.5 Alternative name(s): Isoleucine--tRNA ligase Short name=IleRS | ||||
| Gene names |
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| Encoded on | Plasmid | ||||
| Organism | Staphylococcus aureus | ||||
| Taxonomic identifier | 1280 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Staphylococcus |
Protein attributes
| Sequence length | 1024 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. Confers high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis. HAMAP MF_02003 |
| Catalytic activity | ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003 |
| Cofactor | Zinc By similarity. |
| Subunit structure | Monomer By similarity. |
| Subcellular location | |
| Domain | IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. |
| Sequence similarities | Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antibiotic resistance Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Metal-binding Nucleotide-binding Zinc |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| Technical term | Plasmid |
| Gene Ontology (GO) | |
| Biological process | isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP response to antibioticInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activityInferred from electronic annotation. Source: HAMAP zinc ion bindingInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1024 | 1024 | Isoleucyl-tRNA synthetase HAMAP MF_02003 | PRO_0000098562 | |||||
Regions | |||||||||
| Motif | 42 – 52 | 11 | "HIGH" region HAMAP MF_02003 | ||||||
| Motif | 585 – 589 | 5 | "KMSKS" region HAMAP MF_02003 | ||||||
Sites | |||||||||
| Binding site | 588 | 1 | ATP By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 519 | 1 | P → R Ref.2 | ||||||
| Sequence conflict | 591 – 593 | 3 | GNV → ETF in CAA42080. Ref.2 | ||||||
Sequences
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References
| [1] | "Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870." Hodgson J.E., Curnock S.P., Dyke K.G.H., Morris R., Sylvester D.R., Gross M.S. Antimicrob. Agents Chemother. 38:1205-1208(1994) [PubMed: 8067768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: J2870. |
| [2] | "Cloning of the gene conferring resistance to mupirocin in Staphylococcus aureus." Dyke K.G.H., Curnock S.P., Golding M., Noble W.C. FEMS Microbiol. Lett. 61:195-198(1991) [PubMed: 1903747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 504-519 AND 550-609. Strain: J2870. |
Cross-references
Sequence databases | |
|---|---|
| X75439 Genomic DNA. Translation: CAA53189.1. X59478 Genomic DNA. Translation: CAA42080.1. X59477 Genomic DNA. Translation: CAA42079.1. | |
| RefSeq | YP_001653102.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ILE based on UniProtKB P56690. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 5857592. |
Enzyme and pathway databases | |
| BRENDA | 6.1.1.5. 95. |
Family and domain databases | |
| HAMAP | MF_02003. [Tree] |
| InterPro | IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. |
| PANTHER | PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit. |
| Pfam | PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view] |
| PRINTS | PR00984. TRNASYNTHILE. |
| TIGRFAMs | TIGR00392. ileS. 1 hit. |
| PROSITE | PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SYI2_STAAU | ||||||||
| Accession | Primary (citable) accession number: P41368 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


