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P41368

- SYI2_STAAU

UniProt

P41368 - SYI2_STAAU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).By similarity
Confers high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis.

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zinc.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei588 – 5881ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Antibiotic resistance, Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Synonyms:mupR
Encoded oniPlasmid0 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Isoleucine--tRNA ligasePRO_0000098562Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP41368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi585 – 5895"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41368-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKKYLNTQN EISAFWNTQK IFKKSIDNRK GQESFVFYDG PPTANGLPHA
60 70 80 90 100
GHVLGRVIKD LVARLKTMQG FYVERKAGWD THGLPVELEV EKKIGIKGKQ
110 120 130 140 150
DIEKYGIENF INECKKSVFN YEKEWRDFSK DLGYWVDMDS PYITLENNYI
160 170 180 190 200
ESVWNILSTF HKKGLLYKGH KVTPYCTHDQ TALSSHEVAQ GYKNVKDLSA
210 220 230 240 250
VVKFQLTNSK DTYFLSWTTT PWTLPANVAL AINKDLNYSK IRVENEYYIL
260 270 280 290 300
ATDLINSIIT EKYEIIDTFS GSNLINLKYI PPFESDGLVN AYYVVDGEFV
310 320 330 340 350
TNSEGTGIVH IAPAHGEDDY QLVLERDLDF LNVITREGVY NDRFPELVGN
360 370 380 390 400
KAKNSDIEII KLLSKKQLLY KKQKYEHNYP HCWRCGNPLI YYAMEGWFIK
410 420 430 440 450
TTNFKNEIIN NNNNIEWFPS HIKEGRMGNF LENMVDWNIG RNRYWGTPLN
460 470 480 490 500
VWICNDCNHE YAPSSIKDLQ NNSINKIDED IELHRPYVDN ITLSCPKCNG
510 520 530 540 550
KMSRVEEVID VWFDSGSMPF AQHHYPFDNQ KIFNQHFPAD FIAEGVDQTR
560 570 580 590 600
GWFYSLLVIS TILKGKSSYK RALSLGHILD SNGKKMSKSK GNVINPTELI
610 620 630 640 650
NKYGADSLRW ALISDSAPWN NKRFSENIVA QTKSKFIDTL DNIYKFYNMY
660 670 680 690 700
NKIDHYNPNN EITKSRNTLD NWALSRLNTL IKESNIYVNN YDFTSAARLI
710 720 730 740 750
NEYTNTISNW YIGDSRGRFW EQGISNDKKD AYNTLYEILT TLSRLVAPFV
760 770 780 790 800
PFISEKIHYN LTGKSVHLQD YPQYKESFIN QALEDEMHTV IKIVELSRQA
810 820 830 840 850
RKNADLKIKQ PLSKMVIKPN SQLNLSFLPN YYSIIKDELN IKNIELTDNI
860 870 880 890 900
NDYITYELKL NFSSVGPKLG NKTKNIQTLI DSLSEYDKKS LIESNNFKSL
910 920 930 940 950
SSDAELTKDD FIIKTLPKDS YQLSEDNDCV ILLDKNLSPE LIREGHAREL
960 970 980 990 1000
IRLIQQLRKK KNLPINQRID IYIGVTGELL ESIKTNKNMF KENFVIKNIH
1010 1020
LNVIDEYENT IHFNNKEIKI SLLY
Length:1,024
Mass (Da):118,876
Last modified:November 1, 1995 - v1
Checksum:i56ECD232CA0C8430
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti519 – 5191P → R(PubMed:1903747)Curated
Sequence conflicti591 – 5933GNV → ETF in CAA42080. (PubMed:1903747)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75439 Genomic DNA. Translation: CAA53189.1.
X59478 Genomic DNA. Translation: CAA42080.1.
X59477 Genomic DNA. Translation: CAA42079.1.
RefSeqiWP_012263521.1. NC_010279.1.
YP_001653102.1. NC_010279.1.

Genome annotation databases

GeneIDi5857592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75439 Genomic DNA. Translation: CAA53189.1 .
X59478 Genomic DNA. Translation: CAA42080.1 .
X59477 Genomic DNA. Translation: CAA42079.1 .
RefSeqi WP_012263521.1. NC_010279.1.
YP_001653102.1. NC_010279.1.

3D structure databases

ProteinModelPortali P41368.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5857592.

Phylogenomic databases

eggNOGi COG0060.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870."
    Hodgson J.E., Curnock S.P., Dyke K.G.H., Morris R., Sylvester D.R., Gross M.S.
    Antimicrob. Agents Chemother. 38:1205-1208(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: J2870.
  2. "Cloning of the gene conferring resistance to mupirocin in Staphylococcus aureus."
    Dyke K.G.H., Curnock S.P., Golding M., Noble W.C.
    FEMS Microbiol. Lett. 61:195-198(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 504-519 AND 550-609.
    Strain: J2870.

Entry informationi

Entry nameiSYI2_STAAU
AccessioniPrimary (citable) accession number: P41368
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3