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Protein

Acetyl-CoA acetyltransferase

Gene

ERG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetoacetyl-CoA in the biosynthesis of mevalonate, an intermediate required for the biosynthesis of sterols and nonsterol isoprenoids.

Miscellaneous

Present with 60895 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 acetyl-CoA = CoA + acetoacetyl-CoA.PROSITE-ProRule annotation

Kineticsi

  1. KM=0.16 mM for CoA1 Publication
  2. KM=0.35 mM for acetoacetyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.8.1 Publication

    Pathwayi: (R)-mevalonate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Acetyl-CoA acetyltransferase (ERG10)
    2. Hydroxymethylglutaryl-CoA synthase (ERG13)
    3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (HMG2), 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1)
    This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei91Acyl-thioester intermediateBy similarity1
    Metal bindingi186PotassiumBy similarity1
    Binding sitei186Coenzyme ABy similarity1
    Binding sitei231Coenzyme ABy similarity1
    Metal bindingi248Potassium; via carbonyl oxygenBy similarity1
    Metal bindingi249Potassium; via carbonyl oxygenBy similarity1
    Metal bindingi251Potassium; via carbonyl oxygenBy similarity1
    Binding sitei252Coenzyme ABy similarity1
    Metal bindingi350Potassium; via carbonyl oxygenBy similarity1
    Active sitei354Proton acceptorPROSITE-ProRule annotation1
    Active sitei384Proton acceptorPROSITE-ProRule annotation1

    GO - Molecular functioni

    • acetyl-CoA C-acetyltransferase activity Source: SGD
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • ergosterol biosynthetic process Source: SGD
    • fatty acid beta-oxidation Source: GO_Central

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    LigandMetal-binding, Potassium

    Enzyme and pathway databases

    BioCyciMetaCyc:YPL028W-MONOMER
    YEAST:YPL028W-MONOMER
    ReactomeiR-SCE-191273 Cholesterol biosynthesis
    R-SCE-70895 Branched-chain amino acid catabolism
    R-SCE-77108 Utilization of Ketone Bodies
    R-SCE-77111 Synthesis of Ketone Bodies
    R-SCE-77289 Mitochondrial Fatty Acid Beta-Oxidation
    UniPathwayiUPA00058; UER00101

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-CoA acetyltransferase (EC:2.3.1.9)
    Alternative name(s):
    Acetoacetyl-CoA thiolase
    Ergosterol biosynthesis protein 10
    Gene namesi
    Name:ERG10
    Ordered Locus Names:YPL028W
    ORF Names:LPB3
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XVI

    Organism-specific databases

    EuPathDBiFungiDB:YPL028W
    SGDiS000005949 ERG10

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources1 Publication
    ChainiPRO_00002064162 – 398Acetyl-CoA acetyltransferaseAdd BLAST397

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP41338
    PaxDbiP41338
    PRIDEiP41338

    PTM databases

    iPTMnetiP41338

    Expressioni

    Inductioni

    Induced by low intracellular sterol levels.1 Publication

    Interactioni

    Subunit structurei

    Multimeric.

    Protein-protein interaction databases

    BioGridi36150, 258 interactors
    DIPiDIP-6396N
    IntActiP41338, 9 interactors
    MINTiP41338
    STRINGi4932.YPL028W

    Structurei

    3D structure databases

    ProteinModelPortaliP41338
    SMRiP41338
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiolase family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000009412
    HOGENOMiHOG000012238
    InParanoidiP41338
    KOiK00626
    OMAiFWEREIT
    OrthoDBiEOG092C2K2G

    Family and domain databases

    CDDicd00751 thiolase, 1 hit
    Gene3Di3.40.47.10, 1 hit
    InterProiView protein in InterPro
    IPR002155 Thiolase
    IPR016039 Thiolase-like
    IPR020615 Thiolase_acyl_enz_int_AS
    IPR020610 Thiolase_AS
    IPR020617 Thiolase_C
    IPR020613 Thiolase_CS
    IPR020616 Thiolase_N
    PfamiView protein in Pfam
    PF02803 Thiolase_C, 1 hit
    PF00108 Thiolase_N, 1 hit
    PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits
    TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
    PROSITEiView protein in PROSITE
    PS00098 THIOLASE_1, 1 hit
    PS00737 THIOLASE_2, 1 hit
    PS00099 THIOLASE_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41338-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSQNVYIVST ARTPIGSFQG SLSSKTAVEL GAVALKGALA KVPELDASKD
    60 70 80 90 100
    FDEIIFGNVL SANLGQAPAR QVALAAGLSN HIVASTVNKV CASAMKAIIL
    110 120 130 140 150
    GAQSIKCGNA DVVVAGGCES MTNAPYYMPA ARAGAKFGQT VLVDGVERDG
    160 170 180 190 200
    LNDAYDGLAM GVHAEKCARD WDITREQQDN FAIESYQKSQ KSQKEGKFDN
    210 220 230 240 250
    EIVPVTIKGF RGKPDTQVTK DEEPARLHVE KLRSARTVFQ KENGTVTAAN
    260 270 280 290 300
    ASPINDGAAA VILVSEKVLK EKNLKPLAII KGWGEAAHQP ADFTWAPSLA
    310 320 330 340 350
    VPKALKHAGI EDINSVDYFE FNEAFSVVGL VNTKILKLDP SKVNVYGGAV
    360 370 380 390
    ALGHPLGCSG ARVVVTLLSI LQQEGGKIGV AAICNGGGGA SSIVIEKI
    Length:398
    Mass (Da):41,729
    Last modified:January 23, 2007 - v3
    Checksum:iE835A8E040629A5C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L20428 Genomic DNA Translation: AAA62378.1
    U36624 Genomic DNA Translation: AAB68159.1
    BK006949 Genomic DNA Translation: DAA11401.1
    PIRiA55654
    RefSeqiNP_015297.1, NM_001183842.1

    Genome annotation databases

    EnsemblFungiiYPL028W; YPL028W; YPL028W
    GeneIDi856079
    KEGGisce:YPL028W

    Similar proteinsi

    Entry informationi

    Entry nameiTHIL_YEAST
    AccessioniPrimary (citable) accession number: P41338
    Secondary accession number(s): D6W3Y5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: May 23, 2018
    This is version 169 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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