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Protein

Glutamine synthetase, mitochondrial

Gene
N/A
Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

In liver it is the primary ammonia-fixing/detoxifying enzyme in the mitochondrial matrix and glutamine formed is utilized by carbamoylphosphate synthetase-III which functions to provide urea for osmotic purposes. In kidney; it functions for glutamine synthesis in a substrate cycle between glutamate and glutamine which may be involved in regulation of ammoniagenesis. In spleen the cytosolic enzyme is thought to function in pyrimidine biosynthesis by providing glutamine for carbamoylphosphate synthetase-II. In brain, retina, heart, gill and rectal gland it functions in a biosynthetic capacity.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, mitochondrial (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
OrganismiSqualus acanthias (Spiny dogfish)
Taxonomic identifieri7797 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
BLAST
Chaini15 – 403389Glutamine synthetase, mitochondrialPRO_0000011171Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP41320.
SMRiP41320. Positions 32-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG005847.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Mitochondrial (identifier: P41320-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRICRSFLFL VKKCGNITPT IWRNQHTYKM ATSASANLSK IVKKNYMELP
60 70 80 90 100
QDGKVQAMYI WIDGTGEAVR CKTRTLDNEP KSIAELPEWN FDGSSTYQSE
110 120 130 140 150
GSNSDMYLVP SAMFRDPFRR DPNKLVLCEV LKYNRKPAES NLRHSCQKIM
160 170 180 190 200
SMIANEYPWF GMEQEYTLLG TDGHPFGWPS NCFPGPQGPY YCGVGADKAY
210 220 230 240 250
GRDIVEAHYR ACLYAGIELS GTNAEVMAAQ WEYQVGPCEG IQMGDHLWIS
260 270 280 290 300
RFILHRVCED FGIIASFDPK PIPGNWNGAG CHTNFSTKAM RDDGGLKYIE
310 320 330 340 350
DSIEKLGKRH QYHIRAYDPK GGLDNARALT GHHETSNINE FSAGVANRGA
360 370 380 390 400
SIRIPRSVGQ DKKGYFEDRR PSANCDPYAV TEALVRTCLL DESGDKPIEY

NKN
Length:403
Mass (Da):45,409
Last modified:February 1, 1995 - v1
Checksum:iE003D3F2A33E240B
GO
Isoform Cytoplasmic (identifier: P41320-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Show »
Length:374
Mass (Da):41,872
Checksum:i57476E1C876FCC80
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform Cytoplasmic. CuratedVSP_018735Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04617 mRNA. Translation: AAA61871.1.
PIRiI51326.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04617 mRNA. Translation: AAA61871.1.
PIRiI51326.

3D structure databases

ProteinModelPortaliP41320.
SMRiP41320. Positions 32-400.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005847.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic basis for tissue isozymes of glutamine synthetase in elasmobranchs."
    Laud P.R., Campbell J.W.
    J. Mol. Evol. 39:93-100(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGLNA_SQUAC
AccessioniPrimary (citable) accession number: P41320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.