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P41320

- GLNA_SQUAC

UniProt

P41320 - GLNA_SQUAC

Protein

Glutamine synthetase, mitochondrial

Gene
N/A
Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    In liver it is the primary ammonia-fixing/detoxifying enzyme in the mitochondrial matrix and glutamine formed is utilized by carbamoylphosphate synthetase-III which functions to provide urea for osmotic purposes. In kidney; it functions for glutamine synthesis in a substrate cycle between glutamate and glutamine which may be involved in regulation of ammoniagenesis. In spleen the cytosolic enzyme is thought to function in pyrimidine biosynthesis by providing glutamine for carbamoylphosphate synthetase-II. In brain, retina, heart, gill and rectal gland it functions in a biosynthetic capacity.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase, mitochondrial (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    OrganismiSqualus acanthias (Spiny dogfish)
    Taxonomic identifieri7797 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
    BLAST
    Chaini15 – 403389Glutamine synthetase, mitochondrialPRO_0000011171Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP41320.
    SMRiP41320. Positions 32-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG005847.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Mitochondrial (identifier: P41320-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRICRSFLFL VKKCGNITPT IWRNQHTYKM ATSASANLSK IVKKNYMELP    50
    QDGKVQAMYI WIDGTGEAVR CKTRTLDNEP KSIAELPEWN FDGSSTYQSE 100
    GSNSDMYLVP SAMFRDPFRR DPNKLVLCEV LKYNRKPAES NLRHSCQKIM 150
    SMIANEYPWF GMEQEYTLLG TDGHPFGWPS NCFPGPQGPY YCGVGADKAY 200
    GRDIVEAHYR ACLYAGIELS GTNAEVMAAQ WEYQVGPCEG IQMGDHLWIS 250
    RFILHRVCED FGIIASFDPK PIPGNWNGAG CHTNFSTKAM RDDGGLKYIE 300
    DSIEKLGKRH QYHIRAYDPK GGLDNARALT GHHETSNINE FSAGVANRGA 350
    SIRIPRSVGQ DKKGYFEDRR PSANCDPYAV TEALVRTCLL DESGDKPIEY 400
    NKN 403
    Length:403
    Mass (Da):45,409
    Last modified:February 1, 1995 - v1
    Checksum:iE003D3F2A33E240B
    GO
    Isoform Cytoplasmic (identifier: P41320-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.

    Show »
    Length:374
    Mass (Da):41,872
    Checksum:i57476E1C876FCC80
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2929Missing in isoform Cytoplasmic. CuratedVSP_018735Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04617 mRNA. Translation: AAA61871.1.
    PIRiI51326.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04617 mRNA. Translation: AAA61871.1 .
    PIRi I51326.

    3D structure databases

    ProteinModelPortali P41320.
    SMRi P41320. Positions 32-400.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG005847.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic basis for tissue isozymes of glutamine synthetase in elasmobranchs."
      Laud P.R., Campbell J.W.
      J. Mol. Evol. 39:93-100(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiGLNA_SQUAC
    AccessioniPrimary (citable) accession number: P41320
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3