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P41320 (GLNA_SQUAC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase, mitochondrial

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
OrganismSqualus acanthias (Spiny dogfish)
Taxonomic identifier7797 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In liver it is the primary ammonia-fixing/detoxifying enzyme in the mitochondrial matrix and glutamine formed is utilized by carbamoylphosphate synthetase-III which functions to provide urea for osmotic purposes. In kidney; it functions for glutamine synthesis in a substrate cycle between glutamate and glutamine which may be involved in regulation of ammoniagenesis. In spleen the cytosolic enzyme is thought to function in pyrimidine biosynthesis by providing glutamine for carbamoylphosphate synthetase-II. In brain, retina, heart, gill and rectal gland it functions in a biosynthetic capacity.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Mitochondrion.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative initiation
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: P41320-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic (identifier: P41320-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1414Mitochondrion Potential
Chain15 – 403389Glutamine synthetase, mitochondrial
PRO_0000011171

Natural variations

Alternative sequence1 – 2929Missing in isoform Cytoplasmic.
VSP_018735

Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: E003D3F2A33E240B

FASTA40345,409
        10         20         30         40         50         60 
MRICRSFLFL VKKCGNITPT IWRNQHTYKM ATSASANLSK IVKKNYMELP QDGKVQAMYI 

        70         80         90        100        110        120 
WIDGTGEAVR CKTRTLDNEP KSIAELPEWN FDGSSTYQSE GSNSDMYLVP SAMFRDPFRR 

       130        140        150        160        170        180 
DPNKLVLCEV LKYNRKPAES NLRHSCQKIM SMIANEYPWF GMEQEYTLLG TDGHPFGWPS 

       190        200        210        220        230        240 
NCFPGPQGPY YCGVGADKAY GRDIVEAHYR ACLYAGIELS GTNAEVMAAQ WEYQVGPCEG 

       250        260        270        280        290        300 
IQMGDHLWIS RFILHRVCED FGIIASFDPK PIPGNWNGAG CHTNFSTKAM RDDGGLKYIE 

       310        320        330        340        350        360 
DSIEKLGKRH QYHIRAYDPK GGLDNARALT GHHETSNINE FSAGVANRGA SIRIPRSVGQ 

       370        380        390        400 
DKKGYFEDRR PSANCDPYAV TEALVRTCLL DESGDKPIEY NKN 

« Hide

Isoform Cytoplasmic [UniParc].

Checksum: 57476E1C876FCC80
Show »

FASTA37441,872

References

[1]"Genetic basis for tissue isozymes of glutamine synthetase in elasmobranchs."
Laud P.R., Campbell J.W.
J. Mol. Evol. 39:93-100(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04617 mRNA. Translation: AAA61871.1.
PIRI51326.

3D structure databases

ProteinModelPortalP41320.
SMRP41320. Positions 32-400.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG005847.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_SQUAC
AccessionPrimary (citable) accession number: P41320
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 16, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families