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P41318 (LST8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Target of rapamycin complex subunit LST8

Short name=TORC subunit LST8
Alternative name(s):
Lethal with SEC13 protein 8
Gene names
Name:LST8
Ordered Locus Names:YNL006W
ORF Names:N2005
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity. Ref.4 Ref.5 Ref.7

Subunit structure

The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. LST8 binds to the C-terminal kinase domain in TOR2. Ref.6 Ref.8 Ref.10

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side. Note: Also localizes to membranous structures both proximal to, yet distinct from, the plasma membrane as well as within the cell interior, probably endosomal or Golgi membranes. Ref.7 Ref.8

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat LST8 family.

Contains 7 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Target of rapamycin complex subunit LST8
PRO_0000051483

Regions

Repeat1 – 2727WD 1
Repeat30 – 6839WD 2
Repeat73 – 11240WD 3
Repeat114 – 15340WD 4
Repeat157 – 19640WD 5
Repeat205 – 24440WD 6
Repeat248 – 28740WD 7

Experimental info

Mutagenesis1381G → D in LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression. Ref.5
Mutagenesis1461G → E in LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression. Ref.5
Mutagenesis1711G → E in LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression. Ref.5
Mutagenesis1811G → E in LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression. Ref.5
Mutagenesis3001L → S in LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P41318 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 536AA1DB8E8151F0

FASTA30334,034
        10         20         30         40         50         60 
MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA GHQNVRLYDI 

        70         80         90        100        110        120 
RTTNPNPVAS FEGHRGNVTS VSFQQDNRWM VTSSEDGTIK VWDVRSPSIP RNYKHNAPVN 

       130        140        150        160        170        180 
EVVIHPNQGE LISCDRDGNI RIWDLGENQC THQLTPEDDT SLQSLSMASD GSMLAAANTK 

       190        200        210        220        230        240 
GNCYVWEMPN HTDASHLKPV TKFRAHSTYI TRILLSSDVK HLATCSADHT ARVWSIDDDF 

       250        260        270        280        290        300 
KLETTLDGHQ RWVWDCAFSA DSAYLVTASS DHYVRLWDLS TREIVRQYGG HHKGAVCVAL 


NDV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
Verhasselt P., Aert R., Voet M., Volckaert G.
Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8."
Roberg K.J., Bickel S., Rowley N., Kaiser C.A.
Genetics 147:1569-1584(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-300.
[5]"RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p."
Liu Z., Sekito T., Epstein C.B., Butow R.A.
EMBO J. 20:7209-7219(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-138; GLY-146; GLY-171 AND GLY-181.
[6]"Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN TORC1 AND TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway."
Chen E.J., Kaiser C.A.
J. Cell Biol. 161:333-347(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TOR1 AND TOR2.
[8]"Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH TOR1 AND TOR2.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Molecular organization of target of rapamycin complex 2."
Wullschleger S., Loewith R., Oppliger W., Hall M.N.
J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH TOR2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77114 Genomic DNA. Translation: CAA54380.1.
Z71282 Genomic DNA. Translation: CAA95865.1.
BK006947 Genomic DNA. Translation: DAA10537.1.
PIRS45461.
RefSeqNP_014392.3. NM_001182845.3.

3D structure databases

ProteinModelPortalP41318.
SMRP41318. Positions 3-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35819. 195 interactions.
DIPDIP-5318N.
IntActP41318. 30 interactions.
MINTMINT-562844.
STRING4932.YNL006W.

Proteomic databases

PaxDbP41318.
PeptideAtlasP41318.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL006W; YNL006W; YNL006W.
GeneID855726.
KEGGsce:YNL006W.

Organism-specific databases

CYGDYNL006w.
SGDS000004951. LST8.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00390000014795.
HOGENOMHOG000208395.
KOK08266.
OMAAGHHTVK.
OrthoDBEOG7C8GSW.

Enzyme and pathway databases

BioCycYEAST:G3O-33048-MONOMER.

Gene expression databases

GenevestigatorP41318.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 5 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980099.
PROP41318.

Entry information

Entry nameLST8_YEAST
AccessionPrimary (citable) accession number: P41318
Secondary accession number(s): D6W1H1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families