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P41318

- LST8_YEAST

UniProt

P41318 - LST8_YEAST

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Protein

Target of rapamycin complex subunit LST8

Gene
LST8, YNL006W, N2005
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity.3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein kinase activator activity Source: SGD

GO - Biological processi

  1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
  2. fungal-type cell wall organization Source: SGD
  3. mitochondria-nucleus signaling pathway Source: SGD
  4. positive regulation of protein kinase activity Source: GOC
  5. regulation of cell growth Source: SGD
  6. signal transduction Source: SGD
  7. TOR signaling Source: SGD
  8. transport Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33048-MONOMER.
ReactomeiREACT_188481. S6K1-mediated signalling.
REACT_188925. CD28 dependent PI3K/Akt signaling.
REACT_188931. PIP3 activates AKT signaling.
REACT_219346. HSF1-dependent transactivation.
REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex subunit LST8
Short name:
TORC subunit LST8
Alternative name(s):
Lethal with SEC13 protein 8
Gene namesi
Name:LST8
Ordered Locus Names:YNL006W
ORF Names:N2005
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL006w.
SGDiS000004951. LST8.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side
Note: Also localizes to membranous structures both proximal to, yet distinct from, the plasma membrane as well as within the cell interior, probably endosomal or Golgi membranes.2 Publications

GO - Cellular componenti

  1. endosome membrane Source: SGD
  2. extrinsic component of cytoplasmic side of plasma membrane Source: SGD
  3. Golgi membrane Source: SGD
  4. TORC1 complex Source: SGD
  5. TORC2 complex Source: SGD
  6. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi138 – 1381G → D in LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
Mutagenesisi146 – 1461G → E in LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
Mutagenesisi171 – 1711G → E in LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
Mutagenesisi181 – 1811G → E in LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
Mutagenesisi300 – 3001L → S in LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Target of rapamycin complex subunit LST8PRO_0000051483Add
BLAST

Proteomic databases

MaxQBiP41318.
PaxDbiP41318.
PeptideAtlasiP41318.

Expressioni

Gene expression databases

GenevestigatoriP41318.

Interactioni

Subunit structurei

The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. LST8 binds to the C-terminal kinase domain in TOR2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AVO1Q082362EBI-28598,EBI-29284
AVO2Q047492EBI-28598,EBI-28131
KOG1P388733EBI-28598,EBI-24864
TOR1P351695EBI-28598,EBI-19374
TOR2P326007EBI-28598,EBI-19385
TSC11P400612EBI-28598,EBI-22621

Protein-protein interaction databases

BioGridi35819. 195 interactions.
DIPiDIP-5318N.
IntActiP41318. 30 interactions.
MINTiMINT-562844.
STRINGi4932.YNL006W.

Structurei

3D structure databases

ProteinModelPortaliP41318.
SMRiP41318. Positions 3-302.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 2727WD 1Add
BLAST
Repeati30 – 6839WD 2Add
BLAST
Repeati73 – 11240WD 3Add
BLAST
Repeati114 – 15340WD 4Add
BLAST
Repeati157 – 19640WD 5Add
BLAST
Repeati205 – 24440WD 6Add
BLAST
Repeati248 – 28740WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat LST8 family.
Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000014795.
HOGENOMiHOG000208395.
KOiK08266.
OMAiAGHHTVK.
OrthoDBiEOG7C8GSW.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41318-1 [UniParc]FASTAAdd to Basket

« Hide

MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA    50
GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRWM VTSSEDGTIK 100
VWDVRSPSIP RNYKHNAPVN EVVIHPNQGE LISCDRDGNI RIWDLGENQC 150
THQLTPEDDT SLQSLSMASD GSMLAAANTK GNCYVWEMPN HTDASHLKPV 200
TKFRAHSTYI TRILLSSDVK HLATCSADHT ARVWSIDDDF KLETTLDGHQ 250
RWVWDCAFSA DSAYLVTASS DHYVRLWDLS TREIVRQYGG HHKGAVCVAL 300
NDV 303
Length:303
Mass (Da):34,034
Last modified:February 1, 1995 - v1
Checksum:i536AA1DB8E8151F0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77114 Genomic DNA. Translation: CAA54380.1.
Z71282 Genomic DNA. Translation: CAA95865.1.
BK006947 Genomic DNA. Translation: DAA10537.1.
PIRiS45461.
RefSeqiNP_014392.3. NM_001182845.3.

Genome annotation databases

EnsemblFungiiYNL006W; YNL006W; YNL006W.
GeneIDi855726.
KEGGisce:YNL006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77114 Genomic DNA. Translation: CAA54380.1 .
Z71282 Genomic DNA. Translation: CAA95865.1 .
BK006947 Genomic DNA. Translation: DAA10537.1 .
PIRi S45461.
RefSeqi NP_014392.3. NM_001182845.3.

3D structure databases

ProteinModelPortali P41318.
SMRi P41318. Positions 3-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35819. 195 interactions.
DIPi DIP-5318N.
IntActi P41318. 30 interactions.
MINTi MINT-562844.
STRINGi 4932.YNL006W.

Proteomic databases

MaxQBi P41318.
PaxDbi P41318.
PeptideAtlasi P41318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL006W ; YNL006W ; YNL006W .
GeneIDi 855726.
KEGGi sce:YNL006W.

Organism-specific databases

CYGDi YNL006w.
SGDi S000004951. LST8.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00390000014795.
HOGENOMi HOG000208395.
KOi K08266.
OMAi AGHHTVK.
OrthoDBi EOG7C8GSW.

Enzyme and pathway databases

BioCyci YEAST:G3O-33048-MONOMER.
Reactomei REACT_188481. S6K1-mediated signalling.
REACT_188925. CD28 dependent PI3K/Akt signaling.
REACT_188931. PIP3 activates AKT signaling.
REACT_219346. HSF1-dependent transactivation.
REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

Miscellaneous databases

NextBioi 980099.
PROi P41318.

Gene expression databases

Genevestigatori P41318.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 5 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 6 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8."
    Roberg K.J., Bickel S., Rowley N., Kaiser C.A.
    Genetics 147:1569-1584(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LEU-300.
  5. "RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p."
    Liu Z., Sekito T., Epstein C.B., Butow R.A.
    EMBO J. 20:7209-7219(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-138; GLY-146; GLY-171 AND GLY-181.
  6. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
    Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
    Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TORC1 AND TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway."
    Chen E.J., Kaiser C.A.
    J. Cell Biol. 161:333-347(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TOR1 AND TOR2.
  8. "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
    Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
    Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH TOR1 AND TOR2.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Molecular organization of target of rapamycin complex 2."
    Wullschleger S., Loewith R., Oppliger W., Hall M.N.
    J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH TOR2.

Entry informationi

Entry nameiLST8_YEAST
AccessioniPrimary (citable) accession number: P41318
Secondary accession number(s): D6W1H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi