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P41318

- LST8_YEAST

UniProt

P41318 - LST8_YEAST

Protein

Target of rapamycin complex subunit LST8

Gene

LST8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase activator activity Source: SGD

    GO - Biological processi

    1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
    2. fungal-type cell wall organization Source: SGD
    3. mitochondria-nucleus signaling pathway Source: SGD
    4. positive regulation of protein kinase activity Source: GOC
    5. regulation of cell growth Source: SGD
    6. signal transduction Source: SGD
    7. TOR signaling Source: SGD
    8. transport Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33048-MONOMER.
    ReactomeiREACT_188481. S6K1-mediated signalling.
    REACT_188925. CD28 dependent PI3K/Akt signaling.
    REACT_188931. PIP3 activates AKT signaling.
    REACT_219346. HSF1-dependent transactivation.
    REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Target of rapamycin complex subunit LST8
    Short name:
    TORC subunit LST8
    Alternative name(s):
    Lethal with SEC13 protein 8
    Gene namesi
    Name:LST8
    Ordered Locus Names:YNL006W
    ORF Names:N2005
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL006w.
    SGDiS000004951. LST8.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Also localizes to membranous structures both proximal to, yet distinct from, the plasma membrane as well as within the cell interior, probably endosomal or Golgi membranes.

    GO - Cellular componenti

    1. endosome membrane Source: SGD
    2. extrinsic component of cytoplasmic side of plasma membrane Source: SGD
    3. Golgi membrane Source: SGD
    4. TORC1 complex Source: SGD
    5. TORC2 complex Source: SGD
    6. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi138 – 1381G → D in LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
    Mutagenesisi146 – 1461G → E in LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
    Mutagenesisi171 – 1711G → E in LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
    Mutagenesisi181 – 1811G → E in LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication
    Mutagenesisi300 – 3001L → S in LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Target of rapamycin complex subunit LST8PRO_0000051483Add
    BLAST

    Proteomic databases

    MaxQBiP41318.
    PaxDbiP41318.
    PeptideAtlasiP41318.

    Expressioni

    Gene expression databases

    GenevestigatoriP41318.

    Interactioni

    Subunit structurei

    The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. LST8 binds to the C-terminal kinase domain in TOR2.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AVO1Q082362EBI-28598,EBI-29284
    AVO2Q047492EBI-28598,EBI-28131
    KOG1P388733EBI-28598,EBI-24864
    TOR1P351695EBI-28598,EBI-19374
    TOR2P326007EBI-28598,EBI-19385
    TSC11P400612EBI-28598,EBI-22621

    Protein-protein interaction databases

    BioGridi35819. 195 interactions.
    DIPiDIP-5318N.
    IntActiP41318. 30 interactions.
    MINTiMINT-562844.
    STRINGi4932.YNL006W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41318.
    SMRiP41318. Positions 3-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 2727WD 1Add
    BLAST
    Repeati30 – 6839WD 2Add
    BLAST
    Repeati73 – 11240WD 3Add
    BLAST
    Repeati114 – 15340WD 4Add
    BLAST
    Repeati157 – 19640WD 5Add
    BLAST
    Repeati205 – 24440WD 6Add
    BLAST
    Repeati248 – 28740WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat LST8 family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00390000014795.
    HOGENOMiHOG000208395.
    KOiK08266.
    OMAiAGHHTVK.
    OrthoDBiEOG7C8GSW.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41318-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA    50
    GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRWM VTSSEDGTIK 100
    VWDVRSPSIP RNYKHNAPVN EVVIHPNQGE LISCDRDGNI RIWDLGENQC 150
    THQLTPEDDT SLQSLSMASD GSMLAAANTK GNCYVWEMPN HTDASHLKPV 200
    TKFRAHSTYI TRILLSSDVK HLATCSADHT ARVWSIDDDF KLETTLDGHQ 250
    RWVWDCAFSA DSAYLVTASS DHYVRLWDLS TREIVRQYGG HHKGAVCVAL 300
    NDV 303
    Length:303
    Mass (Da):34,034
    Last modified:February 1, 1995 - v1
    Checksum:i536AA1DB8E8151F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77114 Genomic DNA. Translation: CAA54380.1.
    Z71282 Genomic DNA. Translation: CAA95865.1.
    BK006947 Genomic DNA. Translation: DAA10537.1.
    PIRiS45461.
    RefSeqiNP_014392.3. NM_001182845.3.

    Genome annotation databases

    EnsemblFungiiYNL006W; YNL006W; YNL006W.
    GeneIDi855726.
    KEGGisce:YNL006W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77114 Genomic DNA. Translation: CAA54380.1 .
    Z71282 Genomic DNA. Translation: CAA95865.1 .
    BK006947 Genomic DNA. Translation: DAA10537.1 .
    PIRi S45461.
    RefSeqi NP_014392.3. NM_001182845.3.

    3D structure databases

    ProteinModelPortali P41318.
    SMRi P41318. Positions 3-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35819. 195 interactions.
    DIPi DIP-5318N.
    IntActi P41318. 30 interactions.
    MINTi MINT-562844.
    STRINGi 4932.YNL006W.

    Proteomic databases

    MaxQBi P41318.
    PaxDbi P41318.
    PeptideAtlasi P41318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL006W ; YNL006W ; YNL006W .
    GeneIDi 855726.
    KEGGi sce:YNL006W.

    Organism-specific databases

    CYGDi YNL006w.
    SGDi S000004951. LST8.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00390000014795.
    HOGENOMi HOG000208395.
    KOi K08266.
    OMAi AGHHTVK.
    OrthoDBi EOG7C8GSW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33048-MONOMER.
    Reactomei REACT_188481. S6K1-mediated signalling.
    REACT_188925. CD28 dependent PI3K/Akt signaling.
    REACT_188931. PIP3 activates AKT signaling.
    REACT_219346. HSF1-dependent transactivation.
    REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

    Miscellaneous databases

    NextBioi 980099.
    PROi P41318.

    Gene expression databases

    Genevestigatori P41318.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 5 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
      Verhasselt P., Aert R., Voet M., Volckaert G.
      Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8."
      Roberg K.J., Bickel S., Rowley N., Kaiser C.A.
      Genetics 147:1569-1584(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LEU-300.
    5. "RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p."
      Liu Z., Sekito T., Epstein C.B., Butow R.A.
      EMBO J. 20:7209-7219(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLY-138; GLY-146; GLY-171 AND GLY-181.
    6. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
      Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
      Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN TORC1 AND TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway."
      Chen E.J., Kaiser C.A.
      J. Cell Biol. 161:333-347(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TOR1 AND TOR2.
    8. "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
      Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
      Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH TOR1 AND TOR2.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Molecular organization of target of rapamycin complex 2."
      Wullschleger S., Loewith R., Oppliger W., Hall M.N.
      J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH TOR2.

    Entry informationi

    Entry nameiLST8_YEAST
    AccessioniPrimary (citable) accession number: P41318
    Secondary accession number(s): D6W1H1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 589 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3