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Protein

Target of rapamycin complex subunit LST8

Gene

LST8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle-dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity.3 Publications

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein kinase activator activity Source: SGD

GO - Biological processi

  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • fungal-type cell wall organization Source: SGD
  • mitochondria-nucleus signaling pathway Source: SGD
  • positive regulation of TOR signaling Source: InterPro
  • regulation of actin cytoskeleton organization Source: GO_Central
  • regulation of cell growth Source: SGD
  • signal transduction Source: SGD
  • TOR signaling Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-33048-MONOMER
ReactomeiR-SCE-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex subunit LST8
Short name:
TORC subunit LST8
Alternative name(s):
Lethal with SEC13 protein 8
Gene namesi
Name:LST8
Ordered Locus Names:YNL006W
ORF Names:N2005
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL006W
SGDiS000004951 LST8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138G → D in LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication1
Mutagenesisi146G → E in LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication1
Mutagenesisi171G → E in LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication1
Mutagenesisi181G → E in LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression. 1 Publication1
Mutagenesisi300L → S in LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514831 – 303Target of rapamycin complex subunit LST8Add BLAST303

Proteomic databases

MaxQBiP41318
PaxDbiP41318
PRIDEiP41318

Interactioni

Subunit structurei

The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. LST8 binds to the C-terminal kinase domain in TOR2.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35819, 710 interactors
DIPiDIP-5318N
IntActiP41318, 39 interactors
MINTiP41318
STRINGi4932.YNL006W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6EMKelectron microscopy8.00B/D1-303[»]
ProteinModelPortaliP41318
SMRiP41318
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 27WD 1Add BLAST27
Repeati30 – 68WD 2Add BLAST39
Repeati73 – 112WD 3Add BLAST40
Repeati114 – 153WD 4Add BLAST40
Repeati157 – 196WD 5Add BLAST40
Repeati205 – 244WD 6Add BLAST40
Repeati248 – 287WD 7Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat LST8 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000014795
HOGENOMiHOG000208395
InParanoidiP41318
KOiK08266
OMAiLISCDQA
OrthoDBiEOG092C10HM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR037588 MLST8
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19842 PTHR19842, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P41318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA
60 70 80 90 100
GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRWM VTSSEDGTIK
110 120 130 140 150
VWDVRSPSIP RNYKHNAPVN EVVIHPNQGE LISCDRDGNI RIWDLGENQC
160 170 180 190 200
THQLTPEDDT SLQSLSMASD GSMLAAANTK GNCYVWEMPN HTDASHLKPV
210 220 230 240 250
TKFRAHSTYI TRILLSSDVK HLATCSADHT ARVWSIDDDF KLETTLDGHQ
260 270 280 290 300
RWVWDCAFSA DSAYLVTASS DHYVRLWDLS TREIVRQYGG HHKGAVCVAL

NDV
Length:303
Mass (Da):34,034
Last modified:February 1, 1995 - v1
Checksum:i536AA1DB8E8151F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77114 Genomic DNA Translation: CAA54380.1
Z71282 Genomic DNA Translation: CAA95865.1
BK006947 Genomic DNA Translation: DAA10537.1
PIRiS45461
RefSeqiNP_014392.3, NM_001182845.3

Genome annotation databases

EnsemblFungiiYNL006W; YNL006W; YNL006W
GeneIDi855726
KEGGisce:YNL006W

Similar proteinsi

Entry informationi

Entry nameiLST8_YEAST
AccessioniPrimary (citable) accession number: P41318
Secondary accession number(s): D6W1H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

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