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Protein

Mannose-binding protein C

Gene

Mbl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • complement activation, classical pathway Source: UniProtKB-KW
  • complement activation, lectin pathway Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • innate immune response Source: MGI
  • killing by host of symbiont cells Source: MGI
  • negative regulation of growth of symbiont in host Source: MGI
  • negative regulation of viral process Source: MGI
  • positive regulation of phagocytosis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Complement activation lectin pathway, Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Lectin, Mannose-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-binding protein C
Short name:
MBP-C
Alternative name(s):
Mannan-binding protein
RA-reactive factor P28A subunit
Short name:
RARF/P28A
Gene namesi
Name:Mbl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96924. Mbl2.

Subcellular locationi

GO - Cellular componenti

  • collagen trimer Source: UniProtKB-KW
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 244226Mannose-binding protein CPRO_0000017405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 – 29InterchainPROSITE-ProRule annotation
Disulfide bondi34 – 34InterchainPROSITE-ProRule annotation
Modified residuei43 – 4314-hydroxyprolineSequence Analysis
Modified residuei58 – 5814-hydroxyprolineSequence Analysis
Modified residuei69 – 6914-hydroxyprolineSequence Analysis
Modified residuei78 – 7814-hydroxyprolineSequence Analysis
Modified residuei81 – 8114-hydroxyprolineSequence Analysis
Disulfide bondi151 ↔ 240PROSITE-ProRule annotation
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi218 ↔ 232PROSITE-ProRule annotation

Post-translational modificationi

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

MaxQBiP41317.
PaxDbiP41317.
PRIDEiP41317.

Expressioni

Gene expression databases

BgeeiP41317.
CleanExiMM_MBL2.
ExpressionAtlasiP41317. baseline and differential.
GenevisibleiP41317. MM.

Interactioni

Subunit structurei

Oligomeric complex of 3 or more homotrimers. Interacts with MASP1 and MASP2 (By similarity). Interacts with MEP1A and MEP1B and may inhibit their catalytic activity (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025797.

Structurei

3D structure databases

ProteinModelPortaliP41317.
SMRiP41317. Positions 106-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 9659Collagen-likeAdd
BLAST
Domaini129 – 241113C-type lectinPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili108 – 12619By similarityAdd
BLAST

Domaini

The coiled-coil domain mediates trimerization.By similarity

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Coiled coil, Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG265984.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP41317.
KOiK03991.
OMAiCSAPAVN.
OrthoDBiEOG7VTDPW.
PhylomeDBiP41317.
TreeFamiTF330481.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41317-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIFTSFLLL CVVTVVYAET LTEGVQNSCP VVTCSSPGLN GFPGKDGRDG
60 70 80 90 100
AKGEKGEPGQ GLRGLQGPPG KVGPTGPPGN PGLKGAVGPK GDRGDRAEFD
110 120 130 140 150
TSEIDSEIAA LRSELRALRN WVLFSLSEKV GKKYFVSSVK KMSLDRVKAL
160 170 180 190 200
CSEFQGSVAT PRNAEENSAI QKVAKDIAYL GITDVRVEGS FEDLTGNRVR
210 220 230 240
YTNWNDGEPN NTGDGEDCVV ILGNGKWNDV PCSDSFLAIC EFSD
Length:244
Mass (Da):25,957
Last modified:February 1, 1996 - v2
Checksum:i49AE84E2290DEB0A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31I → L in AAB19343 (PubMed:1712818).Curated
Sequence conflicti15 – 151V → A in AAB19343 (PubMed:1712818).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42294 mRNA. Translation: AAB19343.1.
U09016
, U09013, U09014, U09015 Genomic DNA. Translation: AAA82010.1.
D11440 mRNA. Translation: BAA02005.1.
BC010760 mRNA. Translation: AAH10760.1.
CCDSiCCDS29742.1.
PIRiI48651. LNMSMC.
RefSeqiNP_034906.1. NM_010776.1.
XP_006526793.1. XM_006526730.1.
UniGeneiMm.30045.

Genome annotation databases

EnsembliENSMUST00000025797; ENSMUSP00000025797; ENSMUSG00000024863.
GeneIDi17195.
KEGGimmu:17195.
UCSCiuc008hel.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Mannose-binding protein C

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42294 mRNA. Translation: AAB19343.1.
U09016
, U09013, U09014, U09015 Genomic DNA. Translation: AAA82010.1.
D11440 mRNA. Translation: BAA02005.1.
BC010760 mRNA. Translation: AAH10760.1.
CCDSiCCDS29742.1.
PIRiI48651. LNMSMC.
RefSeqiNP_034906.1. NM_010776.1.
XP_006526793.1. XM_006526730.1.
UniGeneiMm.30045.

3D structure databases

ProteinModelPortaliP41317.
SMRiP41317. Positions 106-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025797.

Proteomic databases

MaxQBiP41317.
PaxDbiP41317.
PRIDEiP41317.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025797; ENSMUSP00000025797; ENSMUSG00000024863.
GeneIDi17195.
KEGGimmu:17195.
UCSCiuc008hel.1. mouse.

Organism-specific databases

CTDi4153.
MGIiMGI:96924. Mbl2.

Phylogenomic databases

eggNOGiNOG265984.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP41317.
KOiK03991.
OMAiCSAPAVN.
OrthoDBiEOG7VTDPW.
PhylomeDBiP41317.
TreeFamiTF330481.

Miscellaneous databases

NextBioi291550.
PROiP41317.
SOURCEiSearch...

Gene expression databases

BgeeiP41317.
CleanExiMM_MBL2.
ExpressionAtlasiP41317. baseline and differential.
GenevisibleiP41317. MM.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the mouse mannose-binding proteins. The mannose-binding protein A but not C is an acute phase reactant."
    Sastry K., Zahedi K., Lelias J.M., Whitehead A.S., Ezekowitz R.A.
    J. Immunol. 147:692-697(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CBA/J.
    Tissue: Liver.
  2. "Characterization of murine mannose-binding protein genes Mbl1 and Mbl2 reveals features common to other collectin genes."
    Sastry R., Wang J.S., Brown D.C., Ezekowitz R.A., Tauber A.I., Sastry K.N.
    Mamm. Genome 6:103-110(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
  3. Kuge S., Ihara S., Watanabe E., Watanabe M., Takishima K., Suga T., Mamaiya G., Kawakami M.
    Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiMBL2_MOUSE
AccessioniPrimary (citable) accession number: P41317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.