Reviewed, UniProtKB/Swiss-Prot P41310 (COX1_DIDMA)

Names and origin

Protein names

Recommended name:
    Cytochrome c oxidase subunit 1
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I

Gene names

Name:	MT-CO1
Synonyms:	COI, COXI, MTCO1

Encoded on

Mitochondrion

Organism

Didelphis marsupialis virginiana (North American opossum)

Taxonomic identifier

9267 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Didelphimorphia › Didelphidae › Didelphis

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Pathway	Energy metabolism; oxidative phosphorylation.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the heme-copper respiratory oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Cytochrome c oxidase subunit 1		PRO_0000183322
Regions
	Transmembrane	17 – 37	21	Potential
	Transmembrane	56 – 76	21	Potential
	Transmembrane	102 – 122	21	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	183 – 203	21	Potential
	Transmembrane	234 – 254	21	Potential
	Transmembrane	268 – 288	21	Potential
	Transmembrane	310 – 330	21	Potential
	Transmembrane	338 – 358	21	Potential
	Transmembrane	380 – 400	21	Potential
	Transmembrane	412 – 432	21	Potential
	Transmembrane	456 – 476	21	Potential
Sites
	Metal binding	61	1	Iron (heme A axial ligand) Probable
	Metal binding	240	1	Copper B Probable
	Metal binding	244	1	Copper B Probable
	Metal binding	290	1	Copper B Probable
	Metal binding	291	1	Copper B Probable
	Metal binding	376	1	Iron (heme A3 axial ligand) Probable
	Metal binding	378	1	Iron (heme A axial ligand) Probable
Amino acid modifications
	Cross-link	240 ↔ 244		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41310-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: FFDAF8036663E8C0	FASTA	513	57,002
	10 20 30 40 50 60 MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSLLIRAE LGQPGTLIGD DQIYNVIVTA 70 80 90 100 110 120 HAFIMIFFMV MPIMIGGFGN WLVPLMIGAP DMAFPRMNNM SFWLLPPSFL LLLASSTIEA 130 140 150 160 170 180 GAGTGWTVYP PLAGNLAHAG ASVDLAIFSL HLAGISSILG AINFITTIIN MKPPAMSQYQ 190 200 210 220 230 240 TPLFVWSVMI TAVLLLLSLP VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH 250 260 270 280 290 300 PEVYILILPG FGMISHIVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGLDVD 310 320 330 340 350 360 TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMLW ALGFIFLFTI GGLTGIVLAN 370 380 390 400 410 420 SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM GGFVHWFPLF TGYMLNDMWA KIHFFIMFVG 430 440 450 460 470 480 VNLTFFPQHF LGLSGMPRRY SDYPDAYTMW NVVSSIGSFI SLTAVILMVF IIWEAFASKR 490 500 510 EVLDVELTTT NIEWLYGCPP PYHTFEQPVF IKA

References

[1]	"The marsupial mitochondrial genome and the evolution of placental mammals." Janke A., Feldmaier-Fuchs G., Thomas K., von Haeseler A., Paabo S. Genetics 137:243-256(1994) [PubMed: 8056314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z29573 Genomic DNA. Translation: CAA82679.1.
PIR	S47872.
RefSeq	NP_007097.1.
3D structure databases
SMR	P41310. Positions 1-511.
ModBase	Search...
Genome annotation databases
GeneID	807779.
Organism-specific databases
CTD	807779.
Phylogenomic databases
HOVERGEN	P41310.
Enzyme and pathway databases
BRENDA	1.9.3.1. 281319.
Family and domain databases
InterPro	IPR000883. Cyt_c_oxidase_su1. [Graphical view]
Gene3D	G3DSA:1.20.210.10. COX1. 1 hit.
PANTHER	PTHR10422. COX1. 1 hit.
Pfam	PF00115. COX1. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
PROSITE	PS50855. COX1. 1 hit. PS00077. COX1_CUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1_DIDMA

Accession

Primary (citable) accession number: P41310

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)