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Protein

Glycerol-1-phosphate phosphohydrolase 1

Gene

GPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major isoform of glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation and required for adaptation to anaerobic conditions.5 Publications

Catalytic activityi

Glycerol 1-phosphate + H2O = glycerol + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=3.1 mM for (DL)-glycerol 3-phosphate1 Publication
  1. Vmax=49 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18NucleophileBy similarity1
Metal bindingi18MagnesiumBy similarity1
Active sitei20Proton donorBy similarity1
Metal bindingi20MagnesiumBy similarity1
Metal bindingi179MagnesiumBy similarity1

GO - Molecular functioni

  • glycerol-1-phosphatase activity Source: SGD
  • magnesium ion binding Source: GO_Central

GO - Biological processi

  • cellular response to osmotic stress Source: GO_Central
  • glycerol biosynthetic process Source: SGD
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YIL053W-MONOMER.
YEAST:YIL053W-MONOMER.
BRENDAi3.1.3.21. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-1-phosphate phosphohydrolase 1Curated (EC:3.1.3.211 Publication)
Alternative name(s):
(DL)-glycerol-3-phosphatase 11 Publication
Related to HOR2 protein 21 Publication
Gene namesi
Name:GPP11 Publication
Synonyms:RHR21 Publication
Ordered Locus Names:YIL053WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL053W.
SGDiS000001315. GPP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to osmosensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000875602 – 250Glycerol-1-phosphate phosphohydrolase 1Add BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate1 Publication
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateCombined sources
Modified residuei90PhosphoserineCombined sources1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP41277.
PRIDEiP41277.
TopDownProteomicsiP41277.

PTM databases

iPTMnetiP41277.

Expressioni

Inductioni

In response to both anaerobic and osmotic stress. Expression seems to be under the control of YIG1.6 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YIG1Q089563EBI-7829,EBI-30385

Protein-protein interaction databases

BioGridi34937. 94 interactors.
DIPiDIP-4713N.
IntActiP41277. 10 interactors.
MINTiMINT-487007.

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 17Combined sources12
Turni21 – 23Combined sources3
Helixi27 – 38Combined sources12
Helixi46 – 52Combined sources7
Helixi58 – 65Combined sources8
Helixi67 – 69Combined sources3
Helixi72 – 80Combined sources9
Helixi82 – 86Combined sources5
Helixi96 – 104Combined sources9
Helixi108 – 110Combined sources3
Beta strandi111 – 114Combined sources4
Helixi119 – 129Combined sources11
Beta strandi135 – 138Combined sources4
Helixi140 – 142Combined sources3
Helixi151 – 159Combined sources9
Helixi169 – 171Combined sources3
Beta strandi174 – 180Combined sources7
Helixi181 – 189Combined sources9
Beta strandi193 – 201Combined sources9
Helixi203 – 206Combined sources4
Beta strandi212 – 217Combined sources6
Helixi218 – 220Combined sources3
Beta strandi221 – 223Combined sources3
Turni228 – 231Combined sources4
Beta strandi232 – 238Combined sources7
Beta strandi241 – 243Combined sources3
Turni245 – 248Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QLTX-ray1.60A1-250[»]
ProteinModelPortaliP41277.
SMRiP41277.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41277.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000065392.
HOGENOMiHOG000248341.
InParanoidiP41277.
KOiK06116.
OMAiEFWRDFG.
OrthoDBiEOG092C47K9.

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLTTKPLSL KINAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI
60 70 80 90 100
HISHGWRTYD AIAKFAPDFA DEEYVNKLEG EIPEKYGEHS IEVPGAVKLC
110 120 130 140 150
NALNALPKEK WAVATSGTRD MAKKWFDILK IKRPEYFITA NDVKQGKPHP
160 170 180 190 200
EPYLKGRNGL GFPINEQDPS KSKVVVFEDA PAGIAAGKAA GCKIVGIATT
210 220 230 240 250
FDLDFLKEKG CDIIVKNHES IRVGEYNAET DEVELIFDDY LYAKDDLLKW
Length:250
Mass (Da):27,947
Last modified:January 23, 2007 - v3
Checksum:i9494B158A937413C
GO

Sequence cautioni

The sequence CAA86169 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91I → IK AA sequence (PubMed:7895733).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50471 mRNA. Translation: BAA09060.1.
Z38060 Genomic DNA. Translation: CAA86169.1. Different initiation.
BK006942 Genomic DNA. Translation: DAA08494.1.
PIRiS48426.
RefSeqiNP_012211.2. NM_001179403.1.

Genome annotation databases

EnsemblFungiiYIL053W; YIL053W; YIL053W.
GeneIDi854758.
KEGGisce:YIL053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50471 mRNA. Translation: BAA09060.1.
Z38060 Genomic DNA. Translation: CAA86169.1. Different initiation.
BK006942 Genomic DNA. Translation: DAA08494.1.
PIRiS48426.
RefSeqiNP_012211.2. NM_001179403.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QLTX-ray1.60A1-250[»]
ProteinModelPortaliP41277.
SMRiP41277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34937. 94 interactors.
DIPiDIP-4713N.
IntActiP41277. 10 interactors.
MINTiMINT-487007.

PTM databases

iPTMnetiP41277.

Proteomic databases

MaxQBiP41277.
PRIDEiP41277.
TopDownProteomicsiP41277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL053W; YIL053W; YIL053W.
GeneIDi854758.
KEGGisce:YIL053W.

Organism-specific databases

EuPathDBiFungiDB:YIL053W.
SGDiS000001315. GPP1.

Phylogenomic databases

GeneTreeiENSGT00530000065392.
HOGENOMiHOG000248341.
InParanoidiP41277.
KOiK06116.
OMAiEFWRDFG.
OrthoDBiEOG092C47K9.

Enzyme and pathway databases

BioCyciMetaCyc:YIL053W-MONOMER.
YEAST:YIL053W-MONOMER.
BRENDAi3.1.3.21. 984.

Miscellaneous databases

EvolutionaryTraceiP41277.
PROiP41277.

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPP1_YEAST
AccessioniPrimary (citable) accession number: P41277
Secondary accession number(s): D6VVM8, P38012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 193000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.