P41273 (TNFL9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor ligand superfamily member 9

Alternative name(s):
4-1BB ligand
Short name=4-1BBL

Gene names

Name:

TNFSF9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Ref.3
Subunit structure	Homotrimer. Ref.3
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed in brain, placenta, lung, skeletal muscle and kidney.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement Ref.1. Source: ProtInc cell proliferation Traceable author statement Ref.1. Source: ProtInc cell-cell signaling Traceable author statement Ref.1. Source: ProtInc immune response Inferred from electronic annotation. Source: InterPro regulation of cell proliferation Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	receptor binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 254

254

Tumor necrosis factor ligand superfamily member 9

PRO_0000185501

Regions

Topological domain

1 – 28

28

Cytoplasmic Potential

Transmembrane

29 – 49

21

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

50 – 254

205

Extracellular Potential

Compositional bias

35 – 41

7

Poly-Leu

Natural variations

Natural variant

17

1

P → A.
Corresponds to variant rs442511 [ dbSNP | Ensembl ].

VAR_011928

Secondary structure

1

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254

Helix Strand Turn

Details...

Beta strand

83 – 85

3

Beta strand

92 – 97

6

Beta strand

104 – 106

3

Beta strand

115 – 117

3

Beta strand

121 – 123

3

Beta strand

133 – 135

3

Beta strand

139 – 151

13

Beta strand

159 – 168

10

Beta strand

178 – 185

8

Beta strand

195 – 206

12

Beta strand

210 – 217

8

Helix

220 – 223

4

Beta strand

226 – 228

3

Beta strand

234 – 239

6

Sequences

	Sequence		Length	Mass (Da)
	P41273 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 827551F34563E508	FASTA	254	26,625
	10 20 30 40 50 60 MEYASDASLD PEAPWPPAPR ARACRVLPWA LVAGLLLLLL LAAACAVFLA CPWAVSGARA 70 80 90 100 110 120 SPGSAASPRL REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL 130 140 150 160 170 180 TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA 190 200 210 220 230 240 LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV 250 TPEIPAGLPS PRSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biological characterization of human 4-1BB and its ligand." Alderson M.R., Smith C.A., Tough T.W., Davis-Smith T., Armitage R.J., Falk B., Roux E., Baker E., Sutherland G.R., Din W.S., Goodwin R.G. Eur. J. Immunol. 24:2219-2227(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"The structure of the trimer of human 4-1BB ligand is unique among members of the tumor necrosis factor superfamily." Won E.Y., Cha K., Byun J.S., Kim D.U., Shin S., Ahn B., Kim Y.H., Rice A.J., Walz T., Kwon B.S., Cho H.S. J. Biol. Chem. 285:9202-9210(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 80-246, FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U03398 mRNA. Translation: AAA53134.1.
BC104805 mRNA. Translation: AAI04806.1.
BC104807 mRNA. Translation: AAI04808.1.

IPI

IPI00013301.

PIR

I38427.

RefSeq

NP_003802.1. NM_003811.3.

UniGene

Hs.1524.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2X29	X-ray	2.30	A	80-246	[»]

ProteinModelPortal

P41273.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3020N.

STRING

9606.ENSP00000245817.

PTM databases

PhosphoSite

P41273.

Polymorphism databases

DMDM

728739.

Proteomic databases

PaxDb

P41273.

PRIDE

P41273.

Protocols and materials databases

DNASU

8744.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000245817; ENSP00000245817; ENSG00000125657.

GeneID

8744.

KEGG

hsa:8744.

UCSC

uc002mfh.2. human.

Organism-specific databases

CTD

8744.

GeneCards

GC19P006531.

HGNC

HGNC:11939. TNFSF9.

HPA

HPA041376.

MIM

606182. gene.

neXtProt

NX_P41273.

PharmGKB

PA36629.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41045.

HOGENOM

HOG000154658.

HOVERGEN

HBG079273.

InParanoid

P41273.

KO

K05472.

OMA

WQLTQGA.

OrthoDB

EOG4MGS8J.

PhylomeDB

P41273.

Gene expression databases

Bgee

P41273.

CleanEx

HS_TNFSF9.

Genevestigator

P41273.

GermOnline

ENSG00000125657. Homo sapiens.

Family and domain databases

Gene3D

2.60.120.40. 1 hit.

InterPro

IPR006052. TNF.
IPR021184. TNF_CS.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]

Pfam

PF00229. TNF. 1 hit.
[Graphical view]

SMART

SM00207. TNF. 1 hit.
[Graphical view]

SUPFAM

SSF49842. TNF_like. 1 hit.

PROSITE

PS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TNFSF9. human.

EvolutionaryTrace

P41273.

GenomeRNAi

8744.

NextBio

32809.

SOURCE

Search...

Entry information

Entry name

TNFL9_HUMAN

Accession

Primary (citable) accession number: P41273
Secondary accession number(s): Q2M3S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.